Proposed mechanism for MhuD-catalyzed heme oxygenation

One of the major challenges for understanding metalloenzyme function is the role of electronic structure. Transition metals have the unique ability to adopt numerous oxidation, spin, and configurational states under ambient conditions and each of these electronic states has unique reactivity. In other words, simply knowing that a metalloprotein contains iron is not enough to predict its chemical reactivity! The Liptak group has developed diverse biochemical (recombinant protein expression, purification, and air-sensitive sample handling), spectroscopic (fluorescence, magnetic circular dichroism, and nuclear magnetic resonance), as well as computational (density functional theory, time-dependent density functional theory, and multi-reference perturbation theory) expertise to address these complex problems.

Proposed mechanism for CfbA-catalyzed nickel insertion

Researchers in the Liptak group learn a variety of biochemical, spectroscopic, and computational skills in addition to management of a research project from the first experiments to journal publication. As a result, graduate student alumni have secured jobs in both academia and industry. The majority of undergraduate alumni have moved on to graduate programs in chemistry.