Wolfgang Dostmann

Home Wolfgang Dostmann Research People Recent Publications Links Contact Us Material Requests University of Vermont College of Medicine Department of Pharmacology 149 Beaumont Avenue HSRF 340 Burlington, VT 05405 USA Phone: (802) 656-5712 Fax: (802) 656-4523	Wolfgang Dostmann
			Wolfgang Dostmann, Ph.D Professor of Pharmacology University of Vermont College of Medicine Health Science Research Facility 330 149 Beaumont Ave Burlington, VT 05405 Vox: (802) 656-0381 Fax: (802) 656-4523 wolfgang.dostmann@uvm.edu
	Academic History:
	INSTITUTION AND LOCATION	DEGREE	YEAR	FIELD OF STUDY
	University of Bremen, Germany	Dipl.Chem. (M.S.)	1983	Chemistry
	University of Bremen, Germany	Dr.rer.nat. (Ph.D.)	1987	Bio-Organic Chemistry
	University of California at San Diego	Post-Doc	1987-1991	Biochemistry & Biophysics
	University of Technology Munich, Germany	Dr.med.habil (M.D.)	1996	Pharmacology & Toxicology

	Full Curriculum Vitae

	Background My research interests focus on the topic of ‘second messenger signaling’ since graduate school in 1982 in Bremen, Germany. First as a chemist, I was involved in the synthesis, rational design, mapping studies, and theoretical aspects of cyclic nucleotides. During graduate school, I also conducted research at Sandoz, Inc (Novartis), New Jersey. As a post-doc at the University of California at San Diego, I concentrated on a wide variety of techniques in molecular biology, protein-biochemistry, and biophysics/crystallography to study the major cyclic nucleotide receptor proteins, namely the cAMP-dependent protein kinase (PKA). After establishing my own laboratory in Munich, Germany, I have expanded my studies to the cGMP-dependent protein kinase (PKG) and its role in disease. At the Technical University of Munich, the pharmacology of cGMP and PKG has become an increasing part of my research interests. Since I have joined the faculty of the Department of Pharmacology at the University of Vermont, my research has continued to develop methods and tools that allow us to study the cGMP-PKG pathway in vitro and in vivo in ever-increasing detail. My research has also allowed me to collaborate with the Pharmaceutical Industry in the development of novel drug targets involving cGMP signaling. Although my research interests are highly focused, my research projects have been and will continue to be stimulated and integrated through the use of a large variety of methods and techniques ranging from organic chemistry, biochemistry, molecular biology, cell biology, biophysics, physiology and pharmacology.
	Selected publications:
	Probing the cyclic nucleotide binding sites of cAMP-dependent protein kinases I and II with analogs of adenosine cyclic-3',5' phosphorothioates Dostmann WRG, Taylor SS, Genieser HG, Jastorff B, Døskeland S and Øgreid D (1990), J Biol Chem, 265: 10484-10491
	Regulatory subunit of protein kinase A: structure of deletion mutant with cAMP binding domains Su Y, Dostmann WRG, Herberg FW, Durick K, Xuong NH, Ten Eyck L, Taylor SS and Varughese KI Science, 269: 807-813, (1995)
	Active site mutations define the pathway for the cooperative activation of cAMP-dependent protein kinase Herberg FW, Taylor SS and Dostmann WRG Biochemistry, 35: 2934-2942, (1996)
	Highly specific, membrane permeant peptide blockers of cGMP-dependent protein kinase Ia inhibit NO-induced cerebral dilation Dostmann WRG, Nickl CK, Taylor MS, Brayden JE, Frank R and Tegge WJ Proc Natl Acad Sci USA, 97: 14772-14777, (2000)
	Spatiotemporal dynamics of guanosine 3',5'-cyclic monophosphate revealed by a genetically encoded, fluorescent indicator Honda A, Adams SR Sawyer CL, Lev-Ram V, Tsien RY and Dostmann WRG Proc Natl Acad Sci USA, 98: 2437-2442, (2001)
	A new class of cGMP-dependent protein kinase peptide inhibitors and their role in elucidating vasomotor mechanisms Dostmann WRG, Nickl, CK, Taylor MS, Brayden JE, Frank R and Tegge WJ Pharm & Ther, 93: 203-215, (2002)
	Unique inhibitory properties of the cell-permeable cGMP-dependent protein kinase inhibitor DT-2 reveal a novel mechanism of vasoregulation Taylor, MS, Okwuchukwuasanya, C, Nickl, CK, Brayden, JE and Dostmann, WRG Mol Pharm, 65: 1111-1119, (2004)
	Cygnets: Rapid cellular internalization of fluorescent cGMP-indicators Honda A, Moosmeier M, and Dostmann WR Front Biosci, 10: 1290-1301, (2005)
	Culture conditions influence uptake and intracellular localization of the membrane permeable cGMP-dependent protein kinase peptide inhibitor DT-2 Foley KF, De Frutos S, Laskovski KE, Tegge W and Dostmann WR Front Biosci 10: 1302-1312, (2005)
	Inhibition of cGMP-dependent protein kinase-I attenuates contractile protein expression and contractile morphology in cultured rat aortic smooth muscle cells Dey N, Foley KF, Lincoln TM and Dostmann WR J Cardiovasc Pharm, 45: 404-413, (2005)
	Compartmentalized phosphodiesterase-2 activity blunts beta-adrenergic cardiac inotropy via an NO/cGMP-dependent pathway Mongillo M, Tocchetti CG, Terrin A, Lissandron V, Cheung YF, Dostmann WR, Pozzan T, Kass DA, Paolocci N, Houslay MD, Zaccolo M Circ Res, 98: 226-234, (2006)
	Dostmann WR. Nitric oxide-evoked transient kinetics of cyclic GMP in vascular smooth muscle cells Cawley SM, Sawyer CL, Brunelle KF, van der Vliet A Cell Signal, 19: 1023-1033, (2007)
	The hinge region operates as a stability switch in cGMP dependent protein kinase I Scholten A, Fuss H, Heck AJ, Dostmann WR FEBS J, 274:2274-2286, (2007)
	Differential patterning of cGMP in vascular smooth muscle cells revealed by single GFP-linked biosensors Nausch L, Ledoux J, Bonev A, Nelson MT, Dostmann WR Proc Natl Acad Sci USA, 105: 365-370 (2008)
	(D)-amino acid analogues of DT-2 as highly selective and superior inhibitors of cGMP-dependent protein kinase I Nickl CK, Raidas SK, Zhao H, Sausbier M, Ruth P, Tegge W, Brayden JE, and Dostmann WR Biochim Biophys Acta, 1804:524-32, (2010)
	Adenosine analogue-oligo-arginine conjugates (ARCs) serve as high-affinity inhibitors and fluorescence probes of type I cGMP-dependent protein kinase (PKGIalpha) Lavogina D, Nickl CK, Enkvist E, Raidaru G, Lust M, Vaasa A, Uri A, Dostmann WR Biochim Biophys Acta. 1804:1857-1868, (2010)
	Exquisite sensitivity to subsecond, picomolar nitric oxide transients conferred on cells byguanylyl cyclase-coupled receptors Batchelor AM, Bartus K, Reynell C, Constantinou S, Halvey EJ, Held KF, Dostmann WR, Vernon J, Garthwaite J Proc Natl Acad Sci U S A. 2010 Dec 21;107(51):22060-5. Epub 2010 Dec 6.
	Crystal structure of cGMP-dependent protein kinase reveals novel site of interchain communication. Osborne BW, Wu J, McFarland CJ, Nickl CK, Sankaran B, Casteel DE, Woods VL Jr, Kornev AP, Taylor SS, Dostmann WR. Structure. 2011 Sep 7;19(9):1317-27.

Academic History:

Background

Selected publications: