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Wolfgang Dostmann

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University of Vermont
College of Medicine
Department of Pharmacology
149 Beaumont Avenue
HSRF 340
Burlington, VT 05405
USA

Phone: (802) 656-5712
Fax: (802) 656-4523

Wolfgang Dostmann

Wolfgang Dostmann, Ph.D
Professor of Pharmacology

University of Vermont
College of Medicine

Health Science Research Facility 330
149 Beaumont Ave
Burlington, VT 05405

Vox: (802) 656-0381
Fax: (802) 656-4523

wolfgang.dostmann@uvm.edu

Academic History:

INSTITUTION AND LOCATIONDEGREEYEARFIELD OF STUDY
University of Bremen, GermanyDipl.Chem. (M.S.)1983Chemistry
University of Bremen, GermanyDr.rer.nat. (Ph.D.)1987Bio-Organic Chemistry
University of California at San DiegoPost-Doc1987-1991Biochemistry & Biophysics
University of Technology Munich, GermanyDr.med.habil (M.D.)1996Pharmacology & Toxicology


Full Curriculum Vitae

Background

My research interests focus on the topic of ‘second messenger signaling’ since graduate school in 1982 in Bremen, Germany. First as a chemist, I was involved in the synthesis, rational design, mapping studies, and theoretical aspects of cyclic nucleotides. During graduate school, I also conducted research at Sandoz, Inc (Novartis), New Jersey.

As a post-doc at the University of California at San Diego, I concentrated on a wide variety of techniques in molecular biology, protein-biochemistry, and biophysics/crystallography to study the major cyclic nucleotide receptor proteins, namely the cAMP-dependent protein kinase (PKA).

After establishing my own laboratory in Munich, Germany, I have expanded my studies to the cGMP-dependent protein kinase (PKG) and its role in disease. At the Technical University of Munich, the pharmacology of cGMP and PKG has become an increasing part of my research interests. Since I have joined the faculty of the Department of Pharmacology at the University of Vermont, my research has continued to develop methods and tools that allow us to study the cGMP-PKG pathway in vitro and in vivo in ever-increasing detail. My research has also allowed me to collaborate with the Pharmaceutical Industry in the development of novel drug targets involving cGMP signaling.

Although my research interests are highly focused, my research projects have been and will continue to be stimulated and integrated through the use of a large variety of methods and techniques ranging from organic chemistry, biochemistry, molecular biology, cell biology, biophysics, physiology and pharmacology.

Selected publications:

Probing the cyclic nucleotide binding sites of cAMP-dependent protein kinases I and II with analogs of adenosine cyclic-3',5' phosphorothioates

Dostmann WRG, Taylor SS, Genieser HG, Jastorff B, Døskeland S and Øgreid D
(1990), J Biol Chem, 265: 10484-10491

Regulatory subunit of protein kinase A: structure of deletion mutant with cAMP binding domains

Su Y, Dostmann WRG, Herberg FW, Durick K, Xuong NH, Ten Eyck L, Taylor SS and Varughese KI
Science, 269: 807-813, (1995)

Active site mutations define the pathway for the cooperative activation of cAMP-dependent protein kinase

Herberg FW, Taylor SS and Dostmann WRG
Biochemistry, 35: 2934-2942, (1996)

Highly specific, membrane permeant peptide blockers of cGMP-dependent protein kinase Ia inhibit NO-induced cerebral dilation

Dostmann WRG, Nickl CK, Taylor MS, Brayden JE, Frank R and Tegge WJ
Proc Natl Acad Sci USA, 97: 14772-14777, (2000)

Spatiotemporal dynamics of guanosine 3',5'-cyclic monophosphate revealed by a genetically encoded, fluorescent indicator

Honda A, Adams SR Sawyer CL, Lev-Ram V, Tsien RY and Dostmann WRG
Proc Natl Acad Sci USA, 98: 2437-2442, (2001)

A new class of cGMP-dependent protein kinase peptide inhibitors and their role in elucidating vasomotor mechanisms

Dostmann WRG, Nickl, CK, Taylor MS, Brayden JE, Frank R and Tegge WJ
Pharm & Ther, 93: 203-215, (2002)

Unique inhibitory properties of the cell-permeable cGMP-dependent protein kinase inhibitor DT-2 reveal a novel mechanism of vasoregulation

Taylor, MS, Okwuchukwuasanya, C, Nickl, CK, Brayden, JE and Dostmann, WRG
Mol Pharm, 65: 1111-1119, (2004)

Cygnets: Rapid cellular internalization of fluorescent cGMP-indicators

Honda A, Moosmeier M, and Dostmann WR
Front Biosci, 10: 1290-1301, (2005)

Culture conditions influence uptake and intracellular localization of the membrane permeable cGMP-dependent protein kinase peptide inhibitor DT-2

Foley KF, De Frutos S, Laskovski KE, Tegge W and Dostmann WR
Front Biosci 10: 1302-1312, (2005)

Inhibition of cGMP-dependent protein kinase-I attenuates contractile protein expression and contractile morphology in cultured rat aortic smooth muscle cells

Dey N, Foley KF, Lincoln TM and Dostmann WR
J Cardiovasc Pharm, 45: 404-413, (2005)

Compartmentalized phosphodiesterase-2 activity blunts beta-adrenergic cardiac inotropy via an NO/cGMP-dependent pathway

Mongillo M, Tocchetti CG, Terrin A, Lissandron V, Cheung YF, Dostmann WR, Pozzan T, Kass DA, Paolocci N, Houslay MD, Zaccolo M
Circ Res, 98: 226-234, (2006)

Dostmann WR. Nitric oxide-evoked transient kinetics of cyclic GMP in vascular smooth muscle cells

Cawley SM, Sawyer CL, Brunelle KF, van der Vliet A
Cell Signal, 19: 1023-1033, (2007)

The hinge region operates as a stability switch in cGMP dependent protein kinase I

Scholten A, Fuss H, Heck AJ, Dostmann WR
FEBS J, 274:2274-2286, (2007)

Differential patterning of cGMP in vascular smooth muscle cells revealed by single GFP-linked biosensors

Nausch L, Ledoux J, Bonev A, Nelson MT, Dostmann WR
Proc Natl Acad Sci USA, 105: 365-370 (2008)

(D)-amino acid analogues of DT-2 as highly selective and superior inhibitors of cGMP-dependent protein kinase I

Nickl CK, Raidas SK, Zhao H, Sausbier M, Ruth P, Tegge W, Brayden JE, and Dostmann WR
Biochim Biophys Acta, 1804:524-32, (2010)

Adenosine analogue-oligo-arginine conjugates (ARCs) serve as high-affinity inhibitors and fluorescence probes of type I cGMP-dependent protein kinase (PKGIalpha)

Lavogina D, Nickl CK, Enkvist E, Raidaru G, Lust M, Vaasa A, Uri A, Dostmann WR
Biochim Biophys Acta. 1804:1857-1868, (2010)

Exquisite sensitivity to subsecond, picomolar nitric oxide transients conferred on cells byguanylyl cyclase-coupled receptors

Batchelor AM, Bartus K, Reynell C, Constantinou S, Halvey EJ, Held KF, Dostmann WR, Vernon J, Garthwaite J
Proc Natl Acad Sci U S A. 2010 Dec 21;107(51):22060-5. Epub 2010 Dec 6.

Crystal structure of cGMP-dependent protein kinase reveals novel site of interchain communication.

Osborne BW, Wu J, McFarland CJ, Nickl CK, Sankaran B, Casteel DE, Woods VL Jr, Kornev AP, Taylor SS, Dostmann WR.
Structure. 2011 Sep 7;19(9):1317-27.