Mass Specs at UVM

Mass Spectrometry at the University of Vermont

In Prof. Matthews' Laboratory, Dept. of Chemistry, Cook Physical Sciences Building

Thermo-Finnigan LCQ Deca XP Ion Trap Mass Spectrometer LCMS

  • Mass separation by a quadrupole ion trap; m/z = 50-2000 & 100-4000 mass ranges
  • ESI and nanospray-ESI interfaces for liquid sample introduction (nanospray is shown in the photo)
  • Thermo-Finnigan Surveyor HPLC system with autosampler and with two quarternary pumps
  • Controlled by the Finnigan XCALIBUR data system on a PC
  • The ion trap can perform MSn CID experiments
  • The system processes peptide data using Thermo-Finnigan SEQUEST as part of the Bioworks package
Finnigan LCQ Deca XP Plus

Thermo-Finnigan MAT DeltaPlus Gas Chromatograph - Combustion - Isotope Ratio Mass Spectrometer (GC-C-IRMS)

  • Mass separation by electromagnet; m/z = 0-70 mass range at 3 kV
  • Triple Faraday-cup collector system for measuring the isotopes of 13C, 18O, or 15N from CO2 or N2 gases
  • Samples injected into the HP6890 GC (with CTC A200S autosampler) then into the Finnigan GCC-III combustion/reduction interface (oxidation oven at 960 °C shown on top of GC)
  • Controlled by Finnigan Isodat data system on a PC
  • Purpose: to measure 13C and 15N isotope ratios from organic compounds eluting from the GC.
  • Precision in the GC continuous-flow mode: <0.5 ‰ for 13C and 15N for <1 and 2 nmol of CO2 and N2 respectively.
Finnigan Delta

Europa Hydra 20/20 Gas Chromatograph - Pyrolysis - Isotope Ratio Mass Spectrometer (GC-P-IRMS)

  • With a HP6890 GC and HP7683 autosampler attached (on the left)
  • Electromagnet analyzer; mass range is m/z = 1-70 at 3 kV
  • Triple Faraday collector system for measuring the isotopes of 13C, 18O, or 15N from CO2, CO, or N2 gases
  • With a Hydra flight tube equipped with a special spur to collect m/z=3 of H2 for 2H measurement in a Faraday cup, while collecting m/z=2 in the regular Faraday cup array. This scheme allows large separation of m/z = 2 & 3 and large separation of m/z = 3 from 4 (from the He carrier gas).
  • Two different pyrolysis interfaces between the GC and IRMS to provide sensitive measurement of 2H and 18O:
    • One to reduce injected water or urine samples to H2 and CO
    • Another to convert organic compounds eluting from the GC to H2 and CO
  • An ABCA-G gas inlet system (not shown to the right) with auto-injector is also installed to process H2 or CO2 gas samples from Exatainer tubes for measurement of 2H, 13C or 18O
Europa 20/20 Hydra

Hewlett Packard HP-5973 Gas Chromatograph - Mass Spectrometer (GCMS)

  • HP6890 GC with an HP7683 autosampler
  • Quadrupole mass analyzer; m/z = 0-800 mass range
  • Electron impact (EI) ionization
  • Negative (NCI) and positive chemical ionization (PCI)
  • Used for measuring stable isotopic enrichments in metabolites of biological samples in the range of 0.5 -10 mole % excess (mpe) tracer by selected ion monitoring (SIM).
HP5973 GCMS

Hewlett Packard HP-5971 GCMS

  • HP5890 GC with an HP7673B autosampler
  • Quadrupole mass analyzer; m/z = 0-650 mass range
  • Electron impact (EI) ionization
  • Used for measuring stable isotopic enrichments in metabolites of biological samples by SIM
HP5971 GCMS

Also in the Dept. of Chemistry, Cook Physical Science Building

Applied Biosystems 4000QTrap Pro

  • High performance hybrid triple-quadrupole/linear ion trap mass spectrometer with collision induced dissociation (CID) capability for MS/MS and for MSn using the ion trap. The ABI 4000QTrap has both the scanning functions of a triple quadrupole with the scanning functions of a linear ion trap that can perform simultaneously in both modes within a single injection/analysis automatically through the software.
  • Operation in both negative and positive ion mode
  • mass range of m/z = 5 to 2,800 in Q1 and Q3 in RF/DC mode and m/z = 70 to 2,800 in linear ion trap mode
  • maximum scan speed of 2,400 amu/sec in RF/DC mode and user-settable scan speeds of 250 amu/sec, 1000 amu/sec and 4,000 amu/sec in linear ion trap mode
  • A number of operational modes are available, including product ion scanning for structural elucidation and precursor ion and neutral loss scanning
  • Three modes of LC spray ionization: (i) atmospheric pressure chemical ionization (APCI), (ii) "Turbo IonSpray", i.e. ESI, and (iii) Nanospray ESI
  • The LC interfaced to the 4000QTrap a Shimadzu with nano-LC capability
  • A full suite of instrument control and data processing software are available
ABI 4000 Qtrap LCMS

Varian Saturn 2100T GCMS

  • Varian 3900 GC and CP-8400 autosampler
  • Ion trap with a m/z = 10-650 mass range
  • Operates in electron impact ionization (EI) and postive chemical ionization (PCI) modes
  • Especially useful in PCI mode is the collisionally induced dissociation (CID) option to acquire MS/MS spectra
Varian Saturn 2100T ion trap

In the Vermont Genetics Network (VGN) Proteomics Facility

Two Thermo-Finnigan LTQ Linear Quadrupole Ion Trap Mass Spectrometers

  • Mass separation by a linear quadrupole ion trap; m/z = 50-2000 & 50-4000 mass ranges
  • ESI & nanospray-ESI interfaces for liquid sample introduction
  • Controlled by the Finnigan XCALIBUR data system
  • The linear ion trap can perform MSn collision induced dissociation (CID) experiments
  • The system processes peptide data using Thermo-Finnigan SEQUEST as part of the Bioworks package
  • Shown in photo: our home-made nanospray stage and microcapillary HPLC column
  • Shimadzu Prominence HPLC system with autosampler and two pumps
Finnigan LTQ LCMS
  • Thermo-Finnigan Surveyor HPLC system with autosampler and quarternary pump
Finnigan LTQ LCMS

Thermo-Finnigan LTQ Orbitrap Discovery Mass Spectrometer LCMS

  • Mass separation by a linear quadrupole ion trap; m/z = 50-2000 & 50-4000 mass ranges
  • and high resolution mass separation by the Orbitrap; resolution 7500-30,000
  • ESI & nanospray-ESI interfaces for liquid sample introduction
  • Controlled by the Finnigan XCALIBUR data system
  • The linear ion trap can perform MSn CID experiments
  • The system processes peptide data using Thermo-Finnigan SEQUEST as part of the Bioworks package
  • Thermo-Finnigan Surveyor HPLC system with autosampler and quarternary pump
Finnigan LTQ-Orbitrap LCMS

In Biochemistry, in the Given Building

Applied Biosystems Voyager-DE Pro Biospectrometry Workstation Matrix-Assisted Laser Desorption-Time of Flight (MALDI-TOF) Mass Spectrometer

  • With a 337-nm N2 3-ns pulse UV laser and delayed extraction after photoionization
  • 1.3-m linear flight tube with a ~300-kDa mass range and a resolution of >2000 for small proteins (m/z <20 kDa) and ~50 for large proteins (m/z ~100 kDa)
  • 2.0-m flight tube in “reflectron” mode with resolution of >7,000 at m/z <6 kDa
  • Positive and negative ion measurement
  • “Post-source decay” capability
MALDI-TOF Under the hood of the MALDI-TOF
Any photo of the MALDI-TOF looks like a boring box, so we have also included an "under the hood" photo the ABI MALDI-TOF DE-Pro with the cover off.