Chem 223 - Lectures

Spring 2016

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  • Introduction to course & mass spectrometry
Dass Chap 1 Intro articles
  • Mass spectrometry - the physics of ions in space
    • definition of mass spectrometry and a mass spectrometer
    • formation & acceleration of ions – basic equations
    • dispersion of ions in a magnetic field – basic equations
    • elementary focusing of beams
Watson Chap 2.II.5.A  
  • Double focusing
    • electric fields and electrostatic focussing
    • combination of electrostatic and magnetic fields
    • mass resolution
de Hoffmann Chap 2.5
Watson Chap 2.III.5.B
McCloskey Chap 2: pp 37-47, Chap 6, & Appendix 1
Ion optics
  • Isotopes and ion abundances
    • exact mass calculations
    • calculating theoretical isotope distributions
    • calculating isotopomer distributions
de Hoffmann Chap 6.3
McCloskey Appendix 4
Isotopes
  • Time of flight (TOF)
de Hoffmann Chap 2.4
Watson 2.III.1
TOF
  • Quadrupole rod mass analyzer
de Hoffmann Chap 2.1
Watson Chap 2.III.2
Quads & ion traps
  • Electron impact (EI) ionization
    • ion source design
    • interpreting EI fragmentation spectra
de Hoffmann Chap 1.1
Watson Chap 6
McCloskey Chap 1: pp 1-10
 
  • Chemical ionization (CI)
    • mechanism
    • source design
    • negative chemical ionization (NCI)
de Hoffmann Chap 1.2
Watson Chap 7
McCloskey Chap 1: pp 10-20
CI
  • Vacuum systems
    • Vacuum pumps
    • Vacuum gauges
Watson Chap 2.VI
McCloskey Chap 3
Vacuum
  • Equations of vacuum: "the arithmetic of emptiness"
  • Simple sample introduction
    • gas & liquid "leaks", solid probes
  Vacuum
  • GCMS interfaces & rudimentary LCMS interfaces
de Hoffmann Chap 5.1
Watson Chap 4, 10, & 11.II
McCloskey Chap 5
 
  • GC &LC atmospheric pressure CI interfaces
de Hoffmann Chap 1.12
Watson Chap 4
APCI
  • Electrospray ionization (ESI)
  • Deconvolution of multiply charged envelopes
de Hoffmann Chap 1.11
Watson Chap 8
ESI
  • Surface ionization
    • fast atom bombardment (FAB)
    • matrix assisted laser desorption ionization (MALDI)
de Hoffmann Chap 1.4
Watson Chap 9
McCloskey Chap 1: pp 21-37, Chap 8, Chap 9, & Appendix 3
FAB
MALDI
  • Detectors
    • Faraday cup
    • Electron multipliers & conversion dynodes
    • Multichannel plates
Watson Chap 2.V
McCloskey Chap 3
 
  • Tandem mass spectrometry - MS/MS
    • Metastable ions & double focusing BE systems
    • post source decay (PSD)
    • CID
      • quadrupole triple-sector instruments
      • hybrids (QTOF)
      • ion trap & MSn
      • FTMS
      • TOF & TOF-TOF
de Hoffmann Chap 4
Watson Chap 3
McCloskey Chap 6 & Chap 10
MS/MS
  • 3D ion trap
de Hoffmann Chap 2.1-2.2.1
Watson Chap 2.III.2.A
Quads & ion traps
  • Linear ion traps (LIT)
de Hoffmann Chap 2.2.2
Watson Chap 2.III.2.B
Quads & ion traps
  • Fourier transform (FT) ion cyclotron resonance (ICR) MS
de Hoffmann Chap 2.6
Watson Chap 2.III.6
FTMS
  • The Orbitrap MS
de Hoffmann Chap 2.3
Watson Chap 2.III.3
Orbitrap
  • Proteins, peptide sequencing & proteomics
    • MALDI/TOF
    • ESI
    • database searching
de Hoffmann Chap 8.2
Watson Chap 12.1-3
McCloskey Chap 12
Proteomics
  • Electron capture dissociation (ECD) & electron transfer dissociation (ETD)
Watson Chap 12.II.5 ECD
ETD