9:30-10:45 AM Tuesdays & Thursdays, Angell B203, August 27 - December 6, 2007.
Text:
No single text has been written that really covers mass spectrometry, but some texts are better than others. One problem is that published material becomes old within about 5 years of publication. Another problem is that most books are shallow in key areas. They are good surveys, but do not cover the details of the field in adequate depth for this course.
One book that is a reasonable introductory source I have found and is the book that is useful to have is Edmond De Hoffmann & Vincent Stroobant, Mass Spectrometry: Principles and Applications,, 3rd ed., John Wiley, 2007
De Hoffmann is available on-line in paperback from a variety of sources including Amazon.Com
Other introductory sources are
C. Dass, Fundamentals of Contemporary Mass Spectrometry, Wiley-Interscience, 2007. New, but shallow and expensive. Available from Amazon.Com
J.Throck Watson & O. David Sparkman, Introduction to Mass Spectrometry: Instrumentation, Applications, and Strategies for Data Interpretation, 4th ed., John Wiley, 2007. The older 3rd edition is in the Cook Library (QP519.9.M3 W37 1997). Available from Amazon.Com.
D.C. Liebler, Introduction to Proteomics: Tools for the New Biology, 2nd ed., Humana Press, 2007. This new 2nd ed available in paperback from Amazon.Com (don't buy the 1st edition if the 2nd is about to be released).
Liebler is a good introduction to proteomics methods by mass spectrometry.
The 2nd edition is due to be shipped by the end of October – before the time we will be getting to proteomics material
For the price, get this book along with the DeHoffmann book
G. Siuzdak, The Expanding Role of Mass Spectrometry in Biotechnology, MCC Press, 2003. Available in paperback from Amazon.Com
Other sources of lecture information during the semester:
Joseph B. Lambert, H.F. Shurvell, D.A. Lightner, & R. G. Cooks, Organic Structural Spectroscopy, Prentice Hall, 1998. Part IV: Mass Spectrometry. In the Cook Library (QD272.S6 O74 1998).
Methods in Enzymology: Mass Spectrometry, edited by James McCloskey, Academic Press. vol. 193, 1990. In the Dana Library under periodicals (shelved by title) and in Bailey/Howe (QP601.M49). Although an older book, it contains a range of basic material by different authors.
See the link on the left to texts on mass spectrometry at UVM.
See the link on the left to the journal club for a current list of journals publishing articles on mass spectrometry that are on-line at UVM.
Instructor:
Dwight Matthews 656-8114 dwight.matthews@uvm.edu
Cook Building - Room A204 Office hours:
Available almost anytime; drop by or call first and/or make an appointment.
How the course grade is determined:
Test 1
25%
Test 2
25%
Student journal club presentation
20%
Journal club participation
5%
Final (covers material from journal club)
25%
Course format:
The 1st part of the course covers introductory basic material about different types of mass spectrometers and their components. There is no such thing as a single mass spectrometer to do mass spectrometry. There is a wide range of different instruments with different methods of producing ions, separating ions, and measuring ions. The hope is that every student taking the course will have a good working knowledge of the fundamentals of mass spectrometry instrumentation when these lectures are complete. This portion of the course is directed towards satisfying analytical chemistry graduate student requirements.
Lectures in the 2nd part of the course will be directed towards proteomics and biological applications of mass spectrometry. This part of the course is directed towards graduate students in the biomedical sciences. However, because the biomedical applications of mass spectrometry are probably the most important applications, this information is also important to analytical chemistry students.
Each day's lecture notes will be available on line as a
PDF file via the link shown on the left
.
In addition, I will have journal articles and other materials available to download that are tied to the lectures. Again see the link on the left.
The 3rd part of the course is a journal club on mass spectrometry
(see link on left)
.
The purpose of the journal club is to cover the most recent mass spectrometry literature relevant to problems and topics of the students in the class. Each of you will select an article from the literature concerning mass spectrometry and present the article to the class.
The presentation should provide background as to why the article is important and what problem it addresses. You will walk the class through the methods used, provide any supplementary information needed to understand the article, discuss the results, and provide your opinion of the strength/weaknesses of the article.
It is assumed that all members of the class will participate in the discussion.
Journal club articles will be approved by me before presentation.
We will have two presentations per 75 min class period (30 min per presentation).
Although the final exam is scheduled by UVM for 8 am Thursday, 12/13/07, the final (like the other exams) will be a take-home exam addressing material from the journal club presentations.
Each student may submit to me 2 written questions as a Word Doc file based upon their journal club presentation as exam questions
Each question needs to be accompanied by a worked answer.
I will select from these questions the material that will go on the final.
The final will be handed out by the afternoon of 12/7/07. It is due back by 5 pm 12/13/07.
See the "Mass spec links" on the left for useful web site links about mass spectrometry.
Any student who finds either a web site that I do not have listed that is useful (and is not a commercial site pushing the benefits of a commercial product) or a relevant book at UVM that I don't have listed will receive 5 exam points added to their grade as a bonus for each web site or book that they turn in and is approved.
