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Invasion 1
(2.3 mb) |
Invasion 4
(4.4 mb) |
Invasion 5
(4.1 mb) |
Invasion of Toxoplasma tachyzoites
into human foreskin fibroblasts (Kim Carey and Gary Ward).
Note the constriction in the parasite's plasma membrane that moves
from anterior to posterior as the parasite penetrates past the host
plasma membrane and into the developing parasitophorous vacuole. Real
time; filmed at 35oC using a Dage VE1000SIT
camera mounted on a Nikon Eclipse E400 microscope (Nomarski optics).
The parasites are ~7um long. |
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Escape 2
(2.8 mb) |
Escape 3
(2.0 mb) |
Escape 5
(2.5 mb) |
Ionophore-induced escape of Toxoplasma tachyzoites
from an infected human foreskin fibroblast (Carolyn Donahue).
Perfusion of 4uM ionomycin across an infected monolayer leads to a
synchronous activation of parasite motility and escape from the parasitophorous
vacuole. Real time; filmed at 35oC using
a Dage VE1000SIT camera mounted on a Nikon Eclipse E400 microscope
(Nomarski optics). The parasites are ~7um long. |
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