Notes on our work with PGIc exon 14-exon16.
We have ten sequences for Polystichum species for Phosphoglucoisomerase from the cytosol (PGIc), using primers that we received from Ishikawa in Japan, who used them for Dryopteris. These primers yield a partial sequence (part of exon 14, all of intron 14, exon 15, intron 15, and part of exon 16). For Polystichum species, this gene has clear, unambiguous sequence, and the data yield enough characters to be useful. At the moment, we are attempting to retrieve multiple clones of the gene with the idea of identifying contributor genomes to hybrids and allopolyploids.
We find PCR conditions to be critical: perhaps because it is a single-copy gene, MgCl2 concentration, quality of storage of plant material, and PCR temperatures are all more importantto control than usual. Here are links to some of our results:
Comparison of the aligned P. concinnum and P. speciosissimum sequences
