FastQCFastQC Report
Mon 14 Sep 2015
poplar_reads1_25000.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamepoplar_reads1_25000.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25000
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCAAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTAT450.18Illumina Paired End PCR Primer 2 (100% over 46bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGGA253.65706E-798.308491
TGGCACA309.313226E-998.308491
GTCGCCT253.65706E-798.308491
CTCTCGC201.4421061E-598.308491
CGTGGAC201.4421061E-598.308491
GCACGAT253.65706E-798.308491
GCGCCGT500.098.308491
GCGCTCA155.707055E-498.308491
GGCTTAT309.313226E-998.308491
TATTCGC155.707055E-498.308491
GCGTCCT253.65706E-798.308491
GCGTACA201.4421061E-598.308491
ATATCGC450.098.308491
CAGTGCC155.707055E-498.308491
CTCGTCG253.65706E-798.308491
CGTCGCC650.098.308481
ACGCGCG650.098.308481
GGCACAG301.1859811E-894.9662
TTCGCCA156.551113E-494.9662
TTCGCAT156.551113E-494.9663
Produced by FastQC (version 0.11.3)