FastQCFastQC Report
Mon 21 Sep 2015
WA_AD4_M2_GTCCGC_L004_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameWA_AD4_M2_GTCCGC_L004_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12401335
Sequences flagged as poor quality0
Sequence length100
%GC39

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTGATCGTGCAAAGGTAGCATAATAATTAGTTTTTTAATTGAGAACTG379390.3059267409516798No Hit
CAGCGTAATTTTTTTTGAGAGTTCTTATCGAAAAAAAAGTTTGCGACCTC302090.24359474201769404No Hit
CCTCGATGTTGGATTAAAATTAACTTTTGGTGTAGAAGCTAAAATTGTTA263610.21256582456646803No Hit
TTTTAATTGAGAACTGGAATGAAAGGTCGAACGAGAAAAAAACTGTCTCG258490.20843723679748993No Hit
CTGGAATGAAAGGTCGAACGAGAAAAAAACTGTCTCGATTTAAATTTAAT243970.19672881992140362No Hit
ATTAAATGGCCGCGATAATTTGATCGTGCAAAGGTAGCATAATAATTAGT238780.19254378661652152No Hit
CTCGGCAAATTTTTTTTCCGCCTGTTTATTAAAAACATGTCTTTTTGATT169640.13679172443934465No Hit
CTCGATGTTGGATTAAAATTAACTTTTGGTGTAGAAGCTAAAATTGTTAG161270.13004245107482382No Hit
AAAAAATTTAATAAACTTTTTTTAATAATAAATCATTGATTTATGATTAA161080.1298892417630844No Hit
ATTTTTTTTGAGAGTTCTTATCGAAAAAAAAGTTTGCGACCTCGATGTTG151920.12250294020764702No Hit
GCGTAATTTTTTTTGAGAGTTCTTATCGAAAAAAAAGTTTGCGACCTCGA146930.11847917986249061No Hit
GTTGGATTAAAATTAACTTTTGGTGTAGAAGCTAAAATTGTTAGGTCTGT138510.11168958825803835No Hit
AAAACATGTCTTTTTGATTATAATTTAAAGTCTGACCTGCCCTATGATAG138380.11158476083421665No Hit
TTTAAAGTCTGACCTGCCCTATGATAGAATTTATTAAATGGCCGCGATAA137300.11071388685169783No Hit
ATTAAATTACCTTAGGGATAACAGCGTAATTTTTTTTGAGAGTTCTTATC133640.10776259168871738No Hit
ATTTTTGAAAGGTTAAGTTAAGGAACTCGGCAAATTTTTTTTCCGCCTGT127930.10315824868854845No Hit
CTTAGGGATAACAGCGTAATTTTTTTTGAGAGTTCTTATCGAAAAAAAAG126840.10227931105804335No Hit
ATTACCTTAGGGATAACAGCGTAATTTTTTTTGAGAGTTCTTATCGAAAA126420.10194063784261936No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTGT13150.025.0807131
GGCTAGT15600.019.9533259
CTGTCAT23100.019.9081331
GACGAGA108650.019.8393894
GTCCCCT24650.019.2242017
AGGCAAG31350.019.1169666
GAGTCCC27500.018.5827225
CTGCGAG27500.018.2430881
AGTCCCC27150.017.9674456
TGGCTAG18900.017.2068488
ACACGCG44950.017.1570858
GTGGGTC16600.016.7922279
CACGCGA49600.016.1106059
GGCAAGT35200.016.1020897
GTGAGCA28650.016.0537197
CGAGCCT24400.015.8018463
CGAGTCC32050.015.7994074
AGTGAGC30600.015.6380526
GAGCCTA24350.015.262774
GTCTAGC6900.014.8108811
Produced by FastQC (version 0.11.3)