FastQCFastQC Report
Mon 21 Sep 2015
WA_AD4_M2_GTCCGC_L004_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameWA_AD4_M2_GTCCGC_L004_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12401335
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGCTTACACCGGTTTAAACTCAGATCATGTAAAATTTTAAAGGTCGAA765810.6175222264377181No Hit
CTCGTCTTTTAAAAATATTTAAGCTTTTTTACTTAAAAATAAAATTCTAA430490.3471319821615979No Hit
CTAAAATATTTTTATTTTTTAAAGATAGAAACCAACCTGGCTTACACCGG430350.34701909108978995No Hit
TTTCATTCCAGTTCTCAATTAAAAAACTAATTATTATGCTACCTTTGCAC391500.31569181866307133No Hit
CAAAAGTTAATTTTAATCCAACATCGAGGTCGCAAACTTTTTTTTCGATA327650.2642054262706394No Hit
GTTTTTTTCTCGTTCGACCTTTCATTCCAGTTCTCAATTAAAAAACTAAT293750.23686965959713208No Hit
CCAAAAGTTAATTTTAATCCAACATCGAGGTCGCAAACTTTTTTTTCGAT286370.2309186873832535No Hit
GTTTAAACTCAGATCATGTAAAATTTTAAAGGTCGAACAGACCTAACAAT264590.21335606206912403No Hit
GCTACCTTTGCACGATCAAATTATCGCGGCCATTTAATAAATTCTATCAT248610.20047035258704002No Hit
CTCAGATCATGTAAAATTTTAAAGGTCGAACAGACCTAACAATTTTAGCT243580.1964143376499385No Hit
GTCTTCTCGTCTTTTAAAAATATTTAAGCTTTTTTACTTAAAAATAAAAT241760.19494675371643455No Hit
CCGGTTTAAACTCAGATCATGTAAAATTTTAAAGGTCGAACAGACCTAAC226470.18261743594540425No Hit
CTTCTCGTCTTTTAAAAATATTTAAGCTTTTTTACTTAAAAATAAAATTC220570.17785988363349592No Hit
CGACCTTTCATTCCAGTTCTCAATTAAAAAACTAATTATTATGCTACCTT214440.172916867417903No Hit
CAACTAAATACTTTTAATTAAATTTTAAAATTAAATTCTTAAACTAAAAA210000.1693366077119923No Hit
CCAACATCGAGGTCGCAAACTTTTTTTTCGATAAGAACTCTCAAAAAAAA205410.16563539328628732No Hit
CTCAATTAAAAAACTAATTATTATGCTACCTTTGCACGATCAAATTATCG194370.1567331259094283No Hit
TTTCGTACTAAAATATTTTTATTTTTTAAAGATAGAAACCAACCTGGCTT180290.14537950954473855No Hit
CTACACCAAAAGTTAATTTTAATCCAACATCGAGGTCGCAAACTTTTTTT155550.12543004442666858No Hit
AAAATATTTAAGCTTTTTTACTTAAAAATAAAATTCTAATTAAATTTAAA147280.11876140754201059No Hit
CTTACACCGGTTTAAACTCAGATCATGTAAAATTTTAAAGGTCGAACAGA136320.10992364934904186No Hit
CGGGAAGCGACTTAGAAGCACTTGCGGTGGACAATGCCTGGGCCGGATTC133860.10793999194441567No Hit
GGGTCTTCTCGTCTTTTAAAAATATTTAAGCTTTTTTACTTAAAAATAAA131540.10606922561159746No Hit
CGTCTTTTAAAAATATTTAAGCTTTTTTACTTAAAAATAAAATTCTAATT129880.10473066004587409No Hit
CGAGACAGTTTTTTTCTCGTTCGACCTTTCATTCCAGTTCTCAATTAAAA124510.10040048107723887No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGTCT33850.028.177761
GACCTAT31450.027.9347329
ATAGACC31200.027.8567336
TAGACCT35400.024.9503367
CCTGGTC42550.023.849327
CTGGTCG42750.023.5180458
CGGGAAG58300.022.6468011
GCTTACA374100.021.7130244
GGCTTAC372100.021.3503023
CTTACAC396450.020.9155525
CTGGCTT387150.020.8017861
TGGCTTA375350.020.652262
TACACCG407300.020.531827
ACACCGG408900.020.4056038
CGACTTA68200.020.1839798
CACCGGT412450.020.1160629
TTACACC419350.019.9638426
ATCCTGG52750.019.9498025
GTCAGTA39450.019.771071
TAATGGA102700.019.39638194
Produced by FastQC (version 0.11.3)