Now lets look at a flat file for delta globin and answer the following questions.

Go to the “Gene” data base, and search for “delta globin”. Choose the HBD link for human delta globin. Find the accession number NM_000519.3 under mRNA and protein. This will bring you to the mRNA flat file for delta globin.  Using this file answer the questions below.

Copy the following questions and paste into a Microsoft Word document. Type the answers in bold after each question. When you have answered each of the questions, print the document and hand it in to your Instructor.

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EXERCISE 1: Genbank. NAME: __________________________________

1. What is the GenBank Accession Number for this gene?

2. What is the gi number of this record?

3. What kind of sequence is this?

4. How many bases are there in the sequence?

5. Who are the authors of the 5^th reference?

6. What journal was the next to the last paper in the reference list published in?

7. What is the abbreviation for the beta globin gene?

8. What is the abbreviation for the delta globin gene?

9. What type of hemoglobin incorporates delta globin chains?

10. What genetic disease is associated with delta globin?

11. What chromosome is this gene located on?

12. Is the gene on the short or long arm of this chromosome?

13. What is the first base of the coding sequence?

14. What is the second base of the third codon?

15. What is the 5^th codon?

16. What is the 6^th amino acid?

17. There are three stop codons. Which one occurs in the gene for delta globin?

18. Where does the poly-A region begin?

19. How many bases are present in this gene?

20. How many bases are present in the coding sequence of this gene?

21. How many amino acids are present in the coding sequence of this gene?

22. The gene begins with base 1, but the CDS does not. EXPLAIN!