Arabidopsis DNA micro-preps for PCR
From Klimyuk et al (1993), Plant Journal 3, 493.
Reagents:
Procedure:
Clip a small piece of leaf tissue (2mm x 2mm) into an eppendorf tube containing 50 ul of 0.25 M NaOH and macerate in NaOH.
DNA can be stored short-term @ 4C, but should be boiled 2 min before using again.