Request for proposals 2013-2014
The Center of Biomedical Research Excellence (COBRE) in Neuroscience is pleased to announce the availability of funds to support new initiatives in neuroscience research at the University of Vermont. The funds are available to support innovative, new research projects from junior and mid-career investigators that require use of one or both of the Neuroscience COBRE multi-user research cores: the Imaging/Physiology Core or the Cellular/Molecular Core.
The focus of the project must align with one of the following strategic research areas supported by the Neuroscience COBRE: 1) Stroke and neurovascular interactions and 2) Neural regulation of autonomic nervous system development, function and disorders.
Junior and mid-career investigators (assistant/associate professor, any track) are eligible to apply; the deadline for receipt of applications is February 22, 2013. Funding is for one year beginning July 1, 2013 and ending June 30, 2014.
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Pilot Projects - 2012 to 2013
Pilot Project 3
“Determinants of multipotency and neurogenesis from reactive astrocytes." Jeffrey L. Spees, Ph.D., Associate Professor of Medicine, Director, Stem Cell Core. Specific Aims: Improved understanding of the mechanisms controlling rad-NSCs and their formation from reactive astrocytes may identify ways to promote multipotency and neurogenesis in situ from reactive astrocytes of peri-infarct tissues after stroke.
Our central hypothesis is that Notch1/HIF1 alpha/EGFR-mediated signals interact to promote Rad-NSC formation from reactive astrocytes by altering promoter methylation status for genes involved in NSC self-renewal, multipotency, and neurogenesis.
Our Specific Aims are:
1. To determine whether EGFR signatling and HIF1 alpha regulate the formation and self renewal of reactive astrocyte-derived Neural Stem Cells (Rad-NCSs). At present, the literature contains no reports of signaling mechanisms that control Rad-NSCs. This Specific Aim seeks to build upon our preliminary data for regulation of Rad-NSCs by Notch1 (Shimada et al., 2012; Accepted with revision).
2. To identify epigenetic targets that change in methylation status in response to Notch1 and EGFR signaling and that may control multipotency and neurogenesis for Rad-NSCs.Whereas epigenetic modifications due to bFGF signaling have been reported for E18 cortical astrocytes (Song and Ghosh, 2004), there are currently no reports in regard to epigenetic changes after Notch1 or EGFR signaling in astrocytes, Rad-NSCs, or SVZ-NSCs.
Last modified January 02 2013 08:45 AM