FY2010 Annual Report Project Narratives

FY2010 Annual Report Project Narratives

AGRICULTURE

Vermont Sustainable Jobs Fund
Vermont Biofuels Initiative - Grass Biomass Energy .

Principal Investigator: S. Bosworth

Accomplishments & Outputs:
Perennial grasses that have traditionally been used for hay, pasture or range have the potential to provide biomass feedstock for energy utilization on marginal land while still protecting soil resources and enhancing wildlife; yet, there is very little information on the productivity and management of perennial grasses grown for biomass feedstock in Vermont. The first objective was to evaluate various native and non-native grasses for biomass production potential on different soils in Vermont. In 2009, two warm-season grass (WSG) and cool season grass (CSG) trials made up of multiple varieties and species were seeded at two locations, South Burlington and Shelburne. In 2010, two additional WSG trials were planted in Randolph Center and Alburgh. Species being evaluated across various trials include Switchgrass, Big bluestem, Indiangrass, Miscanthus x giganteous, Intermediate wheatgrass, Tall wheatgrass, and Reed canarygrass. Most of the 2010 WSG harvests were made late in the fall after this report was submitted; however, from the earliest harvested study, second year stands of Switchgrass averaged 3.2 tons per acre. It usually takes three years for WS grasses to fully mature so yield data will continue to be collected over the next two years or more. Biomass yields of the CSG were collected in late July after they were fully mature. Yields ranged from 1.7 to 2.4 tons of dry matter per acre with Intermediate wheatgrass yielding the highest although there were no statistical differences amongst the treatments. Many of the potential grasses, such as Switchgrass, can be very challenging to establish since their seed can often vary in quality. A second objective was to evaluate a Switchgrass vigor test developed at the University of Massachusetts as a practical method that could be conducted by a producer. The results of this greenhouse method were compared to the results of a seeding rate field trial conducted on a sandy and clay soil, respectively. Based on results so far, we have not found a good relationship between this test and achieving an optimum stand population in the field. By the end of the second year, there was no difference in yield regardless of seeding rate and the studies confirm the recommended seeding rate of 8 to 10 lbs of pure live seed per acre. A third objective was to evaluate the effects of N fertility on the biomass yield of established stands of Reed canarygrass and Switchgrass. Treatments included three levels of N fertility, 0, 50 and 100 lbs of N per acre applied when the grass was about 3 to 4 inches tall. Biomass harvests were made after grasses were fully mature. Results for Reed canarygrass have shown that 50 lbs of N was all that was need and increased the grass biomass by about one ton per acre; however, in 2010, additional N fertilizer did cause some lodging of the stand. Preliminary results so far show Switchgrass has not responded to additional nitrogen fertilizer, which may be explained by the fact that this grass is more efficient at utilizing N from the soil compared to cool season grasses.

Outcomes & Impacts:
The information gained from these field trials will be useful to farmers and land owners who are interested in growing biomass crops, and communities that are developing feasibility studies to evaluate their energy options for the future. What we have learned so far is that with proper field preparation and optimum seeding time, Switchgrass and other warm season grasses can be successfully established and even provide some biomass yield in the first year of production although very low yields. Once established, the costs to produce grass biomass will be relatively low with harvesting and transportation costs making up the greatest proportion of that cost. The major challenge now are the development of a market and continued improvements in boiler technology that can handle the high ash content of grasses.


Hatch
Enhancing farmers capacity to produce high quality organic bread wheat in Vermont.

Principal Investigator: H. Darby

Accomplishments & Outputs:
The demand for local organic food is increasing throughout Vermont. Vermont millers and bakers cannot find enough locally grown organic wheat to meet their increasing demand, and that which is available often does not meet the higher quality standards for bread production. We aim to enhance the capacity of farmers to produce high quality organic wheat to meet the increasing demand from local millers, bakers, and ultimately consumers. The goal is to explore the affects of planting dates on disease and weed incidence as well as wheat yield and flour quality. In addition, a variety of outreach materials and programs will be developed to transfer new knowledge to producers. This project will increase farmers ability to produce bread wheat for an expanding high-value market. In doing so, it will contribute to the long-term profitability and sustainability of organic agriculture and farms in areas like Vermont where farmers do not have the land base to compete on the commodity scale In the spring of 2010 a wheat planting date and harvest date study were established on certified organic land at Borderview Farm in Alburgh, Vermont. The previous crop history for this field site will be organic soybeans in 2009 and hay in 2008. Six planting dates and four spring wheat cultivars were evaluated for their impact on weed and disease incidence as well as yield and quality. The experimental design was a randomized complete block in a split plot arrangement with four replications. Main plots consisted of planting dates and subplots consisted of cultivars. The cultivars were AC McKenzie, AC Superb, Ladoga, and RB07. Planting dates began on April 13th and continued at 1 week intervals until May 21st. Weed biomass and disease incidence data was collected at harvest. Plots were harvested on August 13, 2010. Baking quality data is currently being assessed at the UVM Cereal Quality Lab. Four harvest dates and 4 cultivars were evaluated for their impact on wheat yield and quality. The experimental design was a randomized complete block in a split plot arrangement with four replications. Main plots consisted of variety and subplots consisted of harvest date. The cultivars were AC Bordon, AC Warthog, Harvard, and Arapahoe. Harvest dates began on July 7th and occurred every week until the end of July. Yield, grain moisture, and test weight were recorded at harvest. Baking quality data is currently being assessed at the UVM Cereal Quality Lab. Since this is the first year of the wheat research project there has been little data shared with the agricultural community. However, the UVM Northwest Crops and Soils Team field day held in early August highlighted the project to 225 attendees. The results of the project will be shared this winter to over 150 attendees at the annual UVM Grain Growers Conference. Results will also be posted at www.uvm.edu/extension/cropsoil/. The research will be repeated in 2011.

Outcomes & Impacts:
Preliminary results indicated that the highest yields will be obtained if wheat is planted in April. The May 21st planting date was not harvestable due to significantly high weed biomass. There was a significant difference among the varieties. Interestingly the taller stature varieties yielded significantly higher than shorter stature varieties. Plots with taller varieties also had less weed biomass indicating their ability to compete with the weed population. These results indicate that organic farmers should select taller wheat varieties to have the best chance of higher yielding crops. Since one of the primary production limitations for organic wheat producers these are significant results that will improve farm viability. Farmers can use UVM wheat variety trial results to select taller varieties that perform well in our region. Preliminary results from the harvest date study indicate that farmers harvesting wheat at excessive moistures (greater than 20%) are sacrificing yields. In addition, farmers that leave wheat in the field to dry to storage moistures (12% or less) are also losing yields. Ideally based on the data farmers should be harvesting when the wheat is between 15 and 17% moisture. However, the baking quality data will further indicate proper harvesting conditions.


Hatch/Multistate
Soil Organic Matter: Formation, Function and Management (new project) .

Principal Investigator: J. Gorres

Accomplishments & Outputs:
The purpose of this project is to see how earthworms modify the the amount of organic matter and the structure of soil aggregates (clumps of soils that combine many soil particles in a larger coherent unit) as habitat of other beneficial soil organisms. A greenhouse experiment was conducted in which three earthworm treatments and one control treatment were analyzed for leached nutrients. Analyses of aggregates from these treatments are still to be carried out pending the installation of an instrument that measures the pore structure in individual soil aggregates. Important questions that remain is how organic matter is distributed among aggregates of different sizes and whether that organic matter makes pore structure more inhabitable for soil organisms involved in nutrient cycling.

Outcomes & Impacts:
In a lab experiment, the common night crawler (Lumbricus terrestris) significantly reduces the amount of calcium leached (an element which is an essential element of lime often added to improve soil fertility). The amount of calcium leached from a treatment with a more aggressively invading worm, Amynthas agrestis, on the other hand maintains calcium losses at the level of the control. Lumbricus species, including L terrestris, do not have lungs and carbon dioxide needs to be disposed of in a different form of respiration. Lumbricus species have the ability to fix calcium using a calciferous gland that combines calcium and carbon dioxide to make calcite, a calcium carbonate mineral that is not easily dissolved and leached. This sequesters carbon dioxide from the atmosphere into the soil. How this process changes organic matter in the soil is unknown. Further tests are to be conducted next year. These tests will include measurements of carbon dioxide and methane gases from soils with and without L. terrestris. The outcome of these new experiments may impact the discussion on carbon sequestration with respect to climate change as well as agronomists concerned with calcium availability in soils.


Hatch
Earthworm Mediated Losses of N from Dairy and Maple Production Systems in Vermont .

Principal Investigator: J. Gorres

Accomplishments & Outputs:
Soil fertility and plant nutrition may be affected by the action of earthworms. These organisms are traditionally regarded as positive, but have recently been branded invasive earthworms effecting considerable ecosystem changes in the northeastern United States. Our hypothesis was that earthworms upset the synchronicity of nutrient cycles and plant demand. This project is to evaluate Earthworm communities in maple forests and dairy pastures;how earthworms affect nutrient availability in maple forests; nutrient losses from maple forests and dairy pasture. Significant achievements this year were finishing the first year of sampling sugar bush in Cabot. We also began collecting baseline data on earthworm communities in rotationally grazed land. A graduate student was recruited to conduct field and laboratory experiments regarding nutrient cycling and physical structure of pasture soils. Next year, we shall continue research on earthworm effects in forests and pastures by working on particular findings regarding calcium and nitrogen availability for two kinds of earthworms: those that fix calcium into calcite crystals (limestone) and those that do not. The fixing of calcium may have a feedback on both the productivity and nutrient retention in the ecosystem as well as on the emission of green house gases. In addition, we will collect maple sap from trees in areas affected by earthworms and in areas not affected to test the hypotheses that earthworms are affecting tree physiology. In compacted rotational pastures we will evaluate whether subsoil tillage increases the leaching of nutrients from the rootzone.

Outcomes & Impacts:
To be productive, sugar maples require that nutrients are available in the soil in sufficient amounts. In most organically managed forests, nutrient availability depends on two sources: the natural ability of soils generated from soil minerals and recycling from leaf litter. Nutrient availability has to be balanced so that nutrients are available in appropriate ratios. Earthworms, all invasive species in Vermont, can change the balance and the timing of the release of nutrients. In a maple sugar production forest near Cabot Vermont, we found that the balance of nutrients is distorted when earthworms are present with much more calcium and nitrate, and less potassium available than in a similar area without earthworms. The effect of this distortion on sugar production is as yet unknown as it may take many years for these changes to impact tree species. While, at first sight, greater nitrate and calcium availability may be positive for the nutrition of maples it may also render these ions susceptible to loss by leaching and thus the potential for fertility deficits. In particular, during times before leaf out and after leaf fall, nitrogen concentrations in earthworm soils exceed the values for soils without worms. During these times plants do not pick up nitrogen from the soil and the excess is likely to leach. We found that forests affected by earthworms had less understory plants than those not invaded which is in agreement with observations made in other states. This is usually attributed to the loss of the top soil layer which is a seed bank and regeneration medium for the forest flora. The absence of understory plants may also indicate a tendency of the forest ecosystem to leach excess nutrients. As part of this project we also located three forest sites that have been invaded by the Asian earthworm, Amynthas agrestis, a new threat to Vermont forests hitherto not reported in Vermont. The three sites are in Quechee, South Burlington and Shelburne. This is a significant finding since these worms were thought to require warmer temperatures than those found in Vermont. Amynthas is several times more efficient in using forest resources with observable severe damage done to the forest ecosystem potentially representing a greater threat to biodiversity than European invasive earthworms. We also found Amynthas in several home and institutional gardens to where these worms may have been transported with landscaping materials such as mulch and compost. In an initial survey of rotational pastures deep burrowing earthworms such as the common night crawler (Lumbricus terrestris) were not very abundant. However, species that live in the top 10 cm of the soil, such as pink worm (Apporectodea species) and redworm (Lumbricus rubellus), were found at densities of >200 individuals per square meter. It is mostly deep burrowing worms that affect the loss of nutrients to below the plant root zone. Ultimate beneficiaries of this research are maple syrup producers, dairy farmers and land managers, and potentially policy makers at the national level (climate change, greenhouse gases), and local policy makers (control of invasive earthworms).


Vermont Agency of Agriculture, Food, and Markets
Researching the Taste of Place in Vermont .

Principal Investigator: J. Kolodinsky

Accomplishments & Outputs:
This project is intended to develop producer-driven recommendations for a geographical indication and place-based marketing system for Vermont products. Establishing a system for identifying place-based foods could be a powerful tool for agricultural regions to differentiate themselves in the marketplace. These systems can also help keep food traditions active by adding economic value, fostering communities' pride in their local food culture, and preserving the working landscape. Countries and states around the world have decided that the taste of place is a key strategy for the survival of their small-scale producers. UVM has been working with the Vermont Agency of Agriculture on various aspects of Taste of Place, including a conference with experts from France, Quebec, and Vermont; research visits to Quebec and France; technical sessions with producers in Hardwick; market research studies; and a series of working sessions for producers and other stakeholders. Details and reports on the working sessions are shared with communities of interest on the UVM Food System Research Collaborative website at www.foodsystemresearch.net/taste-of-place/

Outcomes & Impacts:
The working sessions are continuing through the fall. The findings so far indicate a need for: 1) More producers to be part of the conversation; 2) Solidarity within and among the producer organizations; 3) More information about the costs and benefits of a designation system; and 4) A road map of next steps for the process. Upcoming working sessions will address these issues and help the producers take the next steps toward developing recommendations. The recommendations will ultimately be articulated as a systemic method for promoting and preserving Vermont's working landscape, engaging Vermonters and visitors in a meaningful way, and making better use of natural resources. The recommendations will be developed in concert with other agricultural marketing, working landscape, and rural economic development initiatives and be in line with the Farm to Plate recommendations developed by the Vermont Sustainable Jobs Fund. Vermont producers will benefit from the project outcome.


Green Mountain Coffee Roasters, Inc.
Graduate Fellowships for Sustainable Livelihoods in Coffee Producing Regions .

Principal Investigator: E. Mendez

Accomplishments & Outputs:
This project sought to evaluate a food security strategy with coffee farmers in Nicaragua. The general objective is to analyze the relationship between agrobiodiversity and livelihood diversification on levels of food security at the household level. Last summer, graduate student Marcela Pino undertook 100 household surveys that will provide baseline data to be revisited in 2011 or 2012. Ms. Pino went to Nicaragua for 2 months to undertake this research. From this initial activity we have negotiated with CECOCAFE, the cooperative we have been working with to support a survey of the total of their membership (more than 2000 farmers), which we will use to address our research questions, but with a considerable large sample than expected. We hope to conduct surveys of the entire CECOCAFEN membership in the following year. Ms. Pino will finish her MS degree in the spring and the expected outputs are 1 peer reviewed paper, 1 policy level publication and 1 publication to inform farmers.

Outcomes & Impacts:
We are in the process of analyzing findings. We expect the outcomes of this project will provide policy guidelines and implementation strategies to combat food insecurity in coffee growing regions. Direct impact will go to CECOCAFEN and its members, but we envision coffee growers from all over the world could benefit from this research. Direct beneficiaries are coffee farmers and their cooperatives. Indirect beneficiaries are rural development organizations, researchers and institutions. Coffee farmers have no access to research resources, and our support provides necessary information to develop food security projects and better negotiate with external actors.


Earthwatch Institute
Sustainable Coffee Growing Communities in Costa Rica .

Principal Investigator: E. Mendez

Accomplishments & Outputs:
The purpose of this project is to conduct research that supports coffee growers, of the Tarrazu region of Costa Rica, to develop more ecologically sound coffee production strategies. At this stage we have been processing existing information and have decided on developing a research project seeking to reduce nitrogen (N) fertilizer application in coffee farms. The analysis will include soil properties and farm characteristics related to N use and uptake, as well as farmer characteristics that determine heavy N use. Experimental plots in at least 20 farms will be set up next year.

Outcomes & Impacts:
Project outputs so far show that excessive application of N fertilizer is impacting coffee grower profits and also contaminating local watersheds and releasing nitrous oxide to the atmosphere (a greenhouse gas). Our research will contribute to a gap in the literature on coffee production related to N management by small-scale farmers in Costa Rica. We will produce management recommendation strategies for the farmers that will improve N management efficiency and reduce environmental impact. Several hundred farmers in this region, in addition to others around the world will be the potential beneficiaries. Small-scale coffee farmers are resource poor and have little access to agricultural research and extension.


Green Mountain Coffee Roasters, Inc.
Confronting the Thin Months: Participatory Learning on Best Practices and Impacts of the GMCR Food Security Program .

Principal Investigator: E. Mendez

Accomplishments & Outputs:
The project seeks to compare different food security and food sovereignty strategies in Nicaragua and Mexico to identify best practices to achieve food sovereignty for smallholders in coffee growing regions. The project has just started. We got delayed in recruiting a graduate student, but have recruited one to start next January. Next year we will establish contact with coffee cooperatives in Nicaragua and Chiapas, Mexico to start conducting the research.

Outcomes & Impacts:
None so far.


Hatch
Herbaceous Perennials Hardiness .

Principal Investigator: L. Perry

Accomplishments & Outputs:
Many growers of herbaceous perennials in northern climates need to successfully overwinter plants either in production, or held from the previous year. Often this is done in greenhouses which can heat during sunny winter days. This study this past winter continued to address this effect of mid-winter possible deacclimation by warm, or cycling, temperatures. Based on outcomes, a similar study with the same Shasta daisy and a foamflower will be repeated, to get a better underestanding of these effects and on a wider range of species. Results will be shared with industry both in their publications and presentations, and on the author's Perry's Perennial Pages website.

Outcomes & Impacts:
The second winter of this study examined a marginally hardy species of perennial shasta daisy (Becky) and a normally hardy selection of yarrow (Apricot Delight), both popular perennials. Result showed that in midwinter (January), even just 5 days at 16C was enough to deacclimate plants, resulting in loss in subsequent subzero soil temperatures. Returning plants to 4C during the night did not compensate for the deacclimation. There were no differences between one or two weeks held at 16C prior to subsequent freezing. Plants in a normally fluctuating greenhouse (-2 to 8C, mean 4C), continuous 4 or 2C showed no differences, all surviving similarly. This study also supported previous results that plants not sufficiently rooted had poor winter survival, so this study will be repeated one more year. Growers overwintering perennials will benefit from knowing the effect of mid-winter high temperatures and cycling on subsequent plant survival when exposed to freezing. This will enable them to save money either in plant losses, scheduling labor and covering treatments more appropriately, and saving costs and fuel in heating if not needed.


New Hampshire Plant Growers Association
Heuchera (Coralbells) Hardiness .

Principal Investigator: L. Perry

Accomplishments & Outputs:
This project will investigate the cold hardiness of several new cultivars of coralbells (Heuchera villosa) with questionable hardiness. Growers in the northeast have questioned the hardiness of these new introductions, most coming from either the west coast or France breeding programs. Plants of five cultivars (Mocha, Tiramisu, Blackout, Carmel, Frosted Violet) were obtained during summer 2009 as plugs, were grown in quart pots, and were tested during the winter of 2009-2010 in controlled freezing chambers to various temperatures on two dates. Data on survival and regrowth were collected during spring 2010. Results have been placed online, and distributed to growers through articles in newsletters. Five different cultivars will be frozen similarly during winter 2010-2011, and the results compared to these initial five. All will then be compared to two years of field results.

Outcomes & Impacts:
It appears there may be a difference among freezing dates in winter, plants perhaps slightly more hardy and able to withstand low temperatures in mid-December than mid-February. Most were able to withstand a brief (2 hour) exposure to 23F and still have good regrowth. When fully hardened, they were able to withstand much lower (13F) and still be saleable. It was surprising, given questions raised by growers and from earlier field plantings, of this hardiness. The difference and performance of the best was Blackout, and the poorest was Tiramisu, was not surprising given earlier field trials. Results generated from this study will enable growers, and home gardeners, to schedule production protocols to ensure better survival over winter, and home gardeners to choose hardy cultivars more successfully thus avoiding economic loss due to plant losses. Such knowledge on these popular perennials does not currently exist, and is of interest to the industry as witnessed by their funding of this project.

Publications:
Perry, L. Coralbells Hardiness. New Hampshire Plant Growers bulletin, August 2010.


New Hampshire Plant Growers Association
Further Studies on Heuchera (Coralbells) Hardiness.

Principal Investigator: L. Perry

Accomplishments & Outputs:
This project will investigate the cold hardiness of several new cultivars of coralbells (Heuchera villosa) with questionable hardiness. Plants of five cultivars were obtained during summer 2010 as plugs, have been grown in quart pots, and will be tested during the winter of 2010-2011 in controlled freezing chambers to various temperatures. Data on survival and regrowth will be collected during spring 2011. Results will be disseminated to growers through their newsletters, and in presentations and online. Outcomes of this study, coupled with the previous year, should provide a good working base of information for growers of cultivars of this ornamental species.

Outcomes & Impacts:
Results generated from this study in 2011 will enable growers, and home gardeners, to schedule production protocols to ensure better survival over winter, and home gardeners to choose hardy cultivars more successfully thus avoiding economic loss due to plant losses. Such knowledge on these popular perennials does not currently exist.


Hatch
The Vermont Agricultural Labor Force: Characteristics, Challenges, and Policy Implications .

Principal Investigator: D. Baker

Accomplishments & Outputs:
Shortages of domestic farm labor have been common across much of the United States in recent decades. Some sectors of the Vermont agricultural economy, such as apples, have relied on temporary agricultural workers to meet this need. However, until very recently, the most important agricultural sector in the state, dairy, has sourced most of its labor locally. In the past few years an undersupply of local farm workers has been met by new foreign workers, primarily Hispanic, who now work on dairy farms throughout the state. Although recently there has been increasing public awareness of the presence of these workers on Vermont dairy farms, little is known about who these workers are, how they view dairy farm employment, or how they differ from domestic dairy farm workers. This three year project seeks to characterize current agricultural laborers on Vermont farms and the social networks with whom they interact. It intends to improve understanding of the broader issues faced by dairy farm labor in Vermont, including the perspectives of farm managers and farm workers. To understand the different perspectives on the impact of Hispanic labor on dairy farms in Vermont detailed on-farm interviews were conducted with dairy farm managers, domestic workers and Hispanic workers. Surveys were conducted on dairy farms throughout the state that employed at least one full-time employee. A total of 54 workers were surveyed, including 33 Hispanic workers and 21 domestic workers. In addition, 78 farm owners or managers on farms that employed non-family labor were surveyed, of whom 56% hired Hispanic workers. Residents of Vermont were also surveyed through the Vermonter Poll about their views on Hispanic Labor. A total of 649 surveys were conducted, and asked about residents views on how Hispanic workers affected the Vermont agricultural economy and local communities. The results of the survey have been presented to the Vermont Agency of Agriculture, the Northeast Organic Farming Winter Conference and is scheduled to be presented at the Vermont Farm Bureau Annual Meeting on Nov. 5th. A peer-reviewed publication is under review and additional peer-reviewed articles are in process. The results of this study will contribute to a more detailed understanding of the situation faced by the state's farming sector and the policy alternatives available to address agricultural labor issues. The research will result in information of use to other states and regions facing similar changes in their farm labor workforce. The survey results will be disseminated to agricultural interest groups and government policy makers in the coming year to contribute to national, statewide and local discussions about the design of appropriate policies to support a sustainable and just agricultural dairy labor force.

Outcomes & Impacts:
The research surveyed farm owners, farm workers and the general public to gain an understanding of the impact and challenges Vermont faces as it relies more heavily on a Hispanic dairy labor force. More than three-quarters, 78%, of farmers surveyed believed that there is a shortage of domestic labor. Many farmers have responded by hiring Hispanic labor, with more than half of the farms surveyed, 56%, hiring Hispanic workers. Farms surveyed included only farms who hired at least one full-time worker. Collectively respondents employed 443 workers, of whom 33% were Hispanic workers. The trend has been for farms to employ more Hispanic labor and relatively less domestic labor. Among the farms surveyed, five years ago 86% of all employees were from the US, and 14% were Hispanic, Today that has decreased to 69% US and 31% Hispanic. Although most farmers speak little to no Spanish, they are very pleased with their Hispanic workers, believing them to have a better work ethic than their domestic workers (71% excellent for Hispanic workers compared to 41% excellent for domestic workers). The main concern farmers expressed about hiring Hispanic workers was potential legal repercussions. Hispanic workers on dairy farms have significantly less agricultural experience than their domestic counterparts, with a median of 3 years compared to 21 years. Nearly all (91%) of Hispanic workers are primarily employed as milkers, whereas less than half of domestic workers reported this as their primary job. Domestic and Hispanic workers were both satisfied with their jobs and felt they were treated well by their employers, with 90% of respondents in both groups reporting that they were treated well or very well by their employers. Hispanic workers put in more hours than their domestic counterparts each week, 70 compared to 50 hours, and want to put in more hours. Very few Hispanic workers speak English, and 66% say that no one on their farm speaks Spanish. Given this situation, it may not be surprising that the greatest challenge most workers face is isolation (34%). Most Vermont residents (72%) are aware of farm labor issues and have heard something on the news within the past year about this issue. The majority of respondents(86%)believe that undocumented farm workers are helping farmers stay in business. More than 60% believe that these workers are not taking jobs away from Vermonters who want them; most believe that undocumented farm workers are filling jobs that Vermonters don't want(80%). They are more divided in their assessment of whether these workers are a net drain on social services, with a slightly greater percentage (49% compared to 40%) believing workers are not a drain on social services. Dairy farming in Vermont is fundamental to the state's economic future, both directly through the production of milk, and indirectly by maintaining the working Vermont landscape. Hispanic labor is one strategy farmers are using to address the labor crisis. Understanding the characteristics of this labor force can help target assistance to farmers and their workers, and develop rational policies to support a sustainable dairy sector into the future.


Agricultural Research Service/Department of Agriculture
Holstein Cattle Major Histocompatibilty (MHC) Gene Diversity and Tracking the Immune Response to an Adenoviral Vectored Foot and Mouth Disease Vaccine Using MHC Tetramer Technology .

Principal Investigator: J. Barlow

Accomplishments & Outputs:
The purpose of this project is to determine the allele diversity and frequency of the major histocompatibility complex (MHC) class I genes in pure-bred herd of Holstein dairy cattle. These genes are critical in the T cell mediated immune response to intracellular pathogens including viruses. We have adapted molecular methods to determine the class I haplotype of 20 Holstein cattle. We will continue to type this cattle herd and subsequently use these results to develop assays to evaluate how MHC haplotype influences the immune response to Foot and Mouth Virus vaccination.

Outcomes & Impacts:
We have found that all of the 20 cattle typed express at least one of two gene variants demonstrating homogeneity of these immune function genes in this cattle population. These results will contribute to our ability to determine the effect of specific vaccines in preventing disease in populations of dairy cattle. The benefit of this research will be to enhance the understanding of a vaccine's ability to prevent disease, and ultimately lead to improved vaccine development to prevent disease in dairy cattle.


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National Science Foundation
BREAD: A Modern Approach for the Development of Cattle Vaccines for Critical Bovine Diseases Impacting Smallholder Farmers in Sub-Saharan Africa .

Principal Investigator: J. Barlow

Accomplishments & Outputs:
The purpose of this project is to translate new technologies that have been successfully applied in human infectious diseases research to cattle in a way that will significantly improve vaccine technology. This will be accomplished through the analysis of the bovine immune response to foot-and-mouth disease (FMD) and East Coast fever (ECF) using bioinformatics, high-throughput sequencing, peptide synthesis, proteomics and the major histocompatibility complex (MHC) tetramer technology or 'tetramers'. We have identified specific MHC gene sequences and predicted polypeptide binding affinity of potentially important ECF antigens. In the next years we will develop additional MHC molecules and generate tetramers to characterize antigen binding and the immune response to this and additional antigens.

Outcomes & Impacts:
We have shown that the pan-predictive antigen binding model is useful in identifying potentially important antigens that bind to MHC molecules. These initial results will contribute to our ability to describe the effect of the immune system following infection and to determine the effect of specific vaccines in preventing disease in populations of dairy cattle. The benefit of this research will be to enhance the understanding of a vaccine's ability to prevent disease, and ultimately lead to improved vaccine development to prevent disease in dairy cattle.


Merck (John) Fund
The Vermont Institute for Artisan Cheese: Risk Reduction Management Program .

Principal Investigator: C. Donnelly

Accomplishments & Outputs:
The Vermont Institute for Artisan Cheese (VIAC) offers cheesemakers comprehensive education, informed by research, to ameliorate microbiological risks in the cheesemaking environment and produce cheeses of consistent high quality through education on microbiological risk management and good manufacturing practices, process control, and HACCP. As the nation's first and only comprehensive center devoted to artisan cheese, the Institute supports Vermont's small-scale cheese producers, contributes to the latest scientific research and expertise related to dairy and cheese products, and encourages the sustainability of family farms in Vermont and other rural landscapes nationally and internationally.

Outcomes & Impacts:
In 2010, VIAC offered 56 courses to small scale artisan cheesemakers. These courses were offered under two programs, the Cheesemaking Certificate Program (CC) and the Advanced Cheesemaking Certificate (ACC). To more effectively conduct technology transfer and provide technical assistance, the Vermont Institute for Artisan Cheese (VIAC) increased its educational and outreach focus on cheese compositional analysis and sensory analysis. For the artisan cheese industry, conducting salt in moisture analysis, protein analysis, rheology, and expanded sensory analysis is extremely helpful. We expanded our risk reduction program, helping cheese makers produce microbiologically safe products through process validation, environmental pathogen screening, and hands on technical assistance including farm visits. In the future, we would like to expand our work in the sensory area, forming a sensory panel and acquiring facilities so that we can perform descriptive sensory analysis of Vermont cheeses and use this information to expand the market for these important value-added agricultural products.


Animal Health
Effects of genetic variation in host and pathogen on severity of bovine mastitis .

Principal Investigator: D. Kerr

Accomplishments & Outputs:
Experiments are being conducted with mammary and dermal fibroblast cells to evaluate differential responses to various pathogens and pathogen associated molecular patterns (PAMPS) in vitro. The model will be used to determine if differences exist between strains of bacteria in their ability to invade, and survive within the bovine cells. Additionally, the results from specific pathogens will be evaluated across different animals using our cryopreserved bank of fibroblast cultures from over 100 cows. Differences in invasion potential could highlight pathogens of particular concern, whereas differences between cells of different cows would be suggestive of specific host differences is mastitis susceptibility.

Outcomes & Impacts:
Our research is attempting to define causative reasons for the variable degree of pathogenesis observed in bovine mastitis. This work will potentially uncover new avenues for treatment of this disease. Dairy producers will benefit from increased production of high quality milk. Dairy cows will benefit from more effective treatment protocols.


Hatch/Multistate
Mastitis Resistance to Enhance Dairy Food Safety .

Principal Investigator: D. Kerr

Accomplishments & Outputs:
Experiments are being conducted to evaluate genetic differences between animals in their innate response to infection. A dermal fibroblast model is being used to investigate genetic differences in responses between chronically infected and healthy animals. These animals are being selected from local, commercial herds, on the basis of monthly milk somatic cell counts and bacteriological evaluation. In addition, samples are being obtained from cows from two herds maintained at the University of Minnesota. One herd is composed of conventionally bred animals using semen from currently proven bulls. The other is composed of animals bred using semen from bulls that were popular in the 1960s. Our experiments will test whether selection for high milk production over the last 40 years has impacted the innate immune response. Responses of the in vitro cultured fibroblasts to E. coli and S. aureus will be interrogated with various techniques such as flow cytometry to detect expression of TLR2 and TLR4 (pathogen receptors) on the cell surface, and RT-PCR and microarray analysis to detect differences in gene expression of intracellular signaling molecules. We have recently focused efforts on detecting differences between cultures selected from two cows obtained from the University of Vermont herd. One cow has a much higher response to LPS stimulation than the other. Preliminary findings indicate that TLR4 expression is higher in the high responding culture, while expression of IL-1RN, an anti-inflammatory protein, is lower in the high responding culture. Thus, a high response appears related to both pro- and anti-inflammatory signals. Preliminary data were presented at the 2010 Animal Genomics for Animal Health conference.

Outcomes & Impacts:
Key findings are that the fibroblast model is very responsive to stimulation with mastitis causing pathogens. The culture system allows us to examine differences between animals in the absence of environmental variables. Thus, we hope to be able to identify specific genes that contribute to between-animal differences in mastitis susceptibility. Knowledge of these genes will lead to more accurate selection of disease resistance characteristics of future sires, dams and replacement animals.


National Institute of Food and Agriculture/Department of Agriculture
Effects of Variation in Pathogen Detection and Signaling Pathways on Resistance to Bovine Mastitis .

Principal Investigator: D. Kerr

Accomplishments & Outputs:
Experiments are being conducted to evaluate the hypothesis that polymorphisms in genes associated with pathogen detection and intracellular signaling pathways contribute to differences between cows in their innate resistance to mastitis. We are using a dermal fibroblast model to evaluate between-animal differences in the innate response to E. coli cell wall (LPS) and to live E. coli. The relationship between in vitro and in vivo results is also being determined in both calves and lactating cows. Fibroblast cultures have been established from 15 calves sampled at approximately 5 and 11 months of age. Responses of the fibroblasts to LPS reveal substantial between-animal variation in IL-8 production. This molecule is key to recruiting immune cells to the site of infection. Ranking of the animals was relatively consistent between the two sampling times. The four highest, and four lowest responder calves were subsequently challenged with an intravenous infusion of LPS. The in vivo results are consistent with the in vitro predictions in that serum levels of IL-8 and TNF-alfa were higher in the high responder calves. We have similarly established and challenged fibroblast cultures from lactating cows. The in vitro responses were used to rank the cows from high to low responders. The four highest and four lowest responding cows were subsequently challenged with an intramammary infusion of E. coli. All cows developed clinical mastitis and subsequently cleared the infection in similar fashion. However, the numbers of somatic immune cells in milk remained elevated for a longer period of time in the high responding cows. Thus, high responding animals may not be ideal in terms of rapid return to the production of high quality milk following a course of E. coli mastitis. Preliminary data will be presented at the 2010 CRWAD meeting.

Outcomes & Impacts:
Our preliminary findings are very supportive of the concept of using an animal's skin fibroblast cells to study between animal differences in innate resistance to bacterial infection. By studying cells in a controlled environment we hope to uncover specific genetic differences between animals. This could lead to enhanced, DNA-based techniques to breed animals with enhanced disease resistance.


Hatch
Profitability and Transitional Analysis of Vermont Organic Dairy Farms: Identifying Key Factors for Success .

Principal Investigator: R. Parsons

Accomplishments & Outputs:
The purpose of this project is to continue the assessment of the profitability of Vermont organic dairy farms that has been going on since 2005. Organic dairy has proved to be a viable, profitable, and community supporting option for northeast dairy farms. Since 1994, the number of organic dairy farms in Vermont grew from 2 to 202, now accounting for 20% of dairy farms in Vermont. An ongoing 5 year study in Vermont and Maine examining the profitability of organic dairy farms has found that organic farms have been more profitable than similar sized conventional dairy farms. The study found that the organic dairy farms produced greater net farm revenue than conventional dairy farms in 4 out of the 5 years of the study. Results have been disseminated through dairy farmers, organic dairy organizations, presented at conferences, shared with farm lenders and policymakers. This is the longest organic dairy profitability study in the US. This research is in strong demand by regional ag lenders because there is no other source of data on profitability of organic dairy farms. This is the last year of planned research on Vermont organic dairy farms unless new funding is found. Analysis will be conducted to examine the change in profitability over the 6 years of the study.

Outcomes & Impacts:
The study determined that Vermont organic dairy farms produced greater net farm revenue than neighboring conventional dairy farms in 4 out of the 5 years of the study. Net income was higher per cow and per hundredweight for the organic dairy farms despite tending to have smaller herds and producing less milk per cow. The study found typical organic dairy farm averaging 62 cows per farm and producing 13,660 pounds of milk per cow. In comparison, conventional dairy farms in Vermont average 124 cows per farm and 19,120 pounds of milk per cow. Production cost structure for organic dairy farms differ from conventional dairy farms through required grazing, feeding expensive certified organic feed, and forbidden use of chemical fertilizers and non-organic antibiotics. Research data indicates that organic dairy farm operators are younger and more educated than their conventional neighbors. The majority of the farms converted to organic for economic reasons or due to contract milk price. Major cost centers are purchased feed, repairs and supplies, labor, and utilities, the same as with conventional farms. Profitability is down in 2008 and 2009 due to increased feed costs. In 2009, organic dairy farms faced reduced revenue due to imposed marketing quotas by processors. However, conventional dairy farms faced even greater loss of revenue due to 30 year lows in milk prices and substantially higher feed prices. Results indicate that most organic dairy farms were able to cash flow while conventional dairy farms lost $700-$1000 cash costs per cow. Base organic milk prices are about $27 per cwt with some farms exceeding $30 with extra payments for quality, protein, and butterfat. Organic dairy has proved to be a financial success for many of Vermont's 200 organic dairy farms. Many Vermont organic dairy farms would not be in business without the option to go organic. More than 70% of the dairy farms believe that they would not be in business today if they did had not the option to switch to organic. Many did not have the land base, facilities, financial reserves, or dedication to stay with the 'get bigger' trend of conventional milk production. Rural Vermont has benefitted from organic dairy farms, through their contribution to rural economy, support farm service providers, support local tax revenue, and keep farmland open and productive instead of reverting back to forest, and contributing to Vermont's tourism attractions. Since 2008 the current economic downturn has stopped the 20% annual growth of organic dairy products. As a result, organic dairy farms have faced new market challenges including production quotas, lower prices, and increased marketing costs. However, in June 2010, organic milk sales began rebounding with marketing quotas removed by fall 2010. Organic dairy has proved to be financial success and will likely remain an economic force in Vermont's rural communities. The results of this study are important to farmers, policymakers, farm service businesses, and lenders because the data is not available through any other channel.

Publications:
Parsons, R. 2010. 'Organic Dairy Study Results.' NODPA (Northeast Organic Dairy Producers Association) News. 10-3. http://www.nodpa.com/research_ed_parsons_study_051610.shtml


University of Connecticut
The Economic and Environmental Sustainability of Small and Medium Size Dairy Farms in New England: An Integrated Research-Extension Program .

Principal Investigator: R. Parsons

Accomplishments & Outputs:
This project began January 1, 2010, is a multi-state integrated research and outreach project led by University of Connecticut and includes collaboration with the University of Vermont and University of Maine. The project's primary objectives are: 1) To examine the cost structure and profitability of small and medium sized dairy farms in New England, as well as other factors associated with farm exits from the industry; 2) To examine selected alternative dairy farm management options compatible with the conditions of small and medium sized New England dairy farms; and 3) To analyze the impact of positive and negative externalities on the expected cost structure and profitability of small and medium sized dairy operations. An important feature of the project is the explicit recognition that costs and benefits have internal and external components and that both are important when attempting to measure the contributions of and policy options for dairy farms. Therefore, the proposed design concentrates on both costs and benefits, economic, environmental, and community-valued. This project started on Jan. 1, 2010 and research efforts for the next 2 years are still in design and implementation stage. There are no results to disseminate at this state. Activities for the coming year include implementing the following research activities: Assessing regional dairy economic costs and returns; Examining environmental regulations in New England states; Defining alternative dairy commodity alternatives (i.e., grazing, organic); Assessing different production practices within the region; Developing methods to assess community based amenities and negative externalities.

Outcomes & Impacts:
There are not major findings at this early stage of the project. The project is expected to benefit the remaining New England dairy farms who are under growing economic, environmental, and societal pressures that threaten their regional survival. This is also important to the region's urban areas that are examining methods to preserve open spaces, protect local food production, and protect iconic New England small towns without threatening regional environmental resources.


Hatch
Evaluating resilience of Vermont's dairy industry through simulation modeling .

Principal Investigator: J. Smith

Accomplishments & Outputs:
The overarching objectives of this project are to utilize simulation models to better understand the impact of potential biosecurity risks on the dairy industry in Vermont and to develop strategies for minimizing the consequences of these risks. Highly contagious animal diseases, such as foot-and-mouth disease, that can be spread by indirect as well as direct contact require severe measures to control. A survey instrument to characterize the rate of direct and indirect contacts among dairy farms was developed and distributed to Vermont dairy farmers in 2010. Summarization and analysis of the data is underway.

Outcomes & Impacts:
Milk price fluctuations pose an obvious threat to the sustainability of the dairy industry in Vermont and nationwide; less obvious is the threat posed by "foreign" or "exotic" diseases that persist in other parts of the world and could accidentally or intentionally infect livestock in this country, resulting in the devastation of agricultural communities. Knowledge of baseline Vermont-specific contact rates among farms and how these might change in the face of a highly contagious disease epidemic would inform more accurate modeling of the potential spread of disease and effectiveness of control measures.


National Institute of Food and Agriculture/Department of Agriculture
Costs and Challenges Associated with Developing and Implementing a Community-Wide Biosecurity Plan .

Principal Investigator: J. Smith

Accomplishments & Outputs:
In the United Kingdom in 2001, foot-and-mouth disease devastated the industries with susceptible livestock--cattle, swine, sheep, and goats--at a cost of over $15 billion dollars in a country only slightly larger than New England. Preparedness and proactive response plans are the best ways to reduce the economic, environmental, and social devastation that could be caused by a disaster like that in the US. Through this integrated research and extension project, over the next three-and-half years, we expect to (1) Increase adoption of farm visitor record-keeping tools by increasing awareness of risk in agricultural contact networks, (2) Identify possible incentives to support protective emergency biosecurity protocols, (3) Develop a catalog of educational resources to distribute to farms and agricultural communities in the event of a highly contagious disease outbreak, and (4) Complete a farm-level cost-benefit analysis of implementing strict biosecurity in the face of a highly contagious disease outbreak. In Year 1 we have (1) recruited four case farms to participate in the project, (2) developed a "contact recall challenge" which is being conducted with the assistance of 4-H dairy youth and (3) developed a survey instrument to assess perceptions of the need for biosecurity given a hypothetical disease outbreak scenario. An advisory group consisting of the case farm owners, allied agricultural business personnel, local and state officials has been established to provide feedback and suggestions regarding project activities.

Outcomes & Impacts:
This project is predicated on the assumption that proactive planning and response can enhance the resilience and sustainability of agricultural businesses if disaster strikes. This project will make a difference in the lives of farmers, agricultural stakeholders, and emergency management personnel across Vermont by investigating the feasibility and acceptability of on-farm biosecurity practices, especially in the face of a highly contagious disease emergency.


Walker (George) Milk Research Fund
Vermont Cattle Health Improvement Program .

Principal Investigator: J. Smith

Accomplishments & Outputs:
The Vermont Cattle Health Improvement Program was established in 2002 to educate livestock producers about Johne's disease and support management plan development and implementation consistent with national voluntary program standards. Johne's disease is a Mycobacterial disease of ruminants that is slowly progressive and not readily detected. With support from the Walker Fund, a set of highly visual fact sheets have been developed. These will be distributed to veterinarians to support their efforts to better inform and work with clients to prevent and manage this disease. A web site is also being created where electronic copies of these and related materials will be maintained. In the coming year, veterinarians will be surveyed to assess the usefulness of these materials.

Outcomes & Impacts:
Herds with significant prevalence of Johne's disease will reap economic benefits through higher herd production, improved reproduction, and fewer Johne's-related culls as management changes take effect, but the "payback" may not be seen for a number of years. As fewer funds from any source are being directed to support Johne's disease programs, participation in voluntary programs has decreased. The goal of this project is to further the progress of the Vermont Cattle Health Improvement Program in raising awareness of Johne's disease, assessing its prevalence, and rewarding dairy herds that have achieved or maintained low risk status.


Agricultural Research Service/Department of Agriculture
Pilot Study of Factors Affecting Maintenance of Mycobacterium, Salmonella, E. Coli, and Listeria on Dairy Farms .

Principal Investigator: J. Smith

Accomplishments & Outputs:
This project is part of a multi-state collaborative research study in which disease dynamics are tracked in three well-characterized dairy herds (one each in Vermont, New York, and Pennsylvania). The primary pathogens of interest are Mycobacterium avium paratuberculosis, which causes Johne's disease in cattle, and four food-borne pathogens: Salmonella spp., Escherichia coli O157:H7, Listeria monocytogenes, and Campylobacter spp. In Vermont the project is accomplished by team members from UVM and the Vermont Agency of Agriculture, Food and Markets Animal Health division. The Vermont herd is routinely visited four times a year for collection of blood and fecal samples from individual cows, environmental manure composites, other environmental samples, and herd management information. Data from this herd has supported further documentation of the progression of infection with Johne's disease to "supershedder" status and the relationship of infection status to milk production. The project group regularly publishes manuscripts and presents abstracts at scientific conferences.

Outcomes & Impacts:
The goal of the project is to track disease dynamics in well-characterized dairy herds and use this information to build disease transmission models that can inform management practices on all dairy farms. Data from the Vermont herd has contributed to models of how Johne's "supershedders" affect the risk of disease transmission and milk production within herds as well as the relationship between Johne's disease status and reproduction and culling. Data are also suggesting genetic associations with risk of infection. Strain typing of the Mycobacterium that causes Johne's disease has shown that all of the low shedders present at the same time as a supershedder in the Vermont herd were shedding the same strain and many of the low shedders were confirmed to have been infected with that strain.

Publications:
Smith, R. L., R. L. Strawderman, Y. H. Schukken, S. J. Wells, A. K. Pradhan, L. A. Espejo, R. H. Whitlock, J. S. Van Kessel, J. M. Smith, D. R. Wolfgang, and Y. T. Grohn. 2010. Effect of Johne's disease status on reproduction and culling in dairy cattle. Journal of Dairy Science 93: 3513-3524.

Aly, S. S., B. L. Mangold, R. H. Whitlock, R. W. Sweeney, R. J. Anderson, J. Jiang, Y. Schukken, E. Hovingh, D. Wolfgang, J. S. Van Kessel, J. S. Karns, J. E. Lombard, J. M. Smith, , I. A. Gardner. 2010. Correlation between Herrold's egg yolk medium culture and real-time quantitative polymerase chain reaction results for Mycobacterium avium subspecies paratuberculosis in pooled fecal and environmental samples. Journal of Veterinary Diagnostic Investigation 22: 677-683.


National Institute of Food and Agriculture/Department of Agriculture
Enhancing the Profitability and Sustainability of Small and Medium Sized Dairy Farms through Artisan Cheese and Other Valued-Added Products .

Principal Investigator: Q. Wang

Accomplishments & Outputs:
The project goal is to provide technical and business management information and training for assisting small and medium dairy farms to produce and market farmstead and artisan cheese and other value-added dairy products through integrated research, extension, and education activities. Most previous programs for dairy farmers have focused on milk productivity, but increased milk production has failed to increase farm income. This project focuses on the potentials of farmstead and artisan cheese and other dairy products that have shown significant increase in consumer demand. We capitalize on the success of the Vermont Institute for Artisan Cheese (VIAC), which has trained more than 900 farmers and other individuals since 2004, to develop extension and education programs for helping farmers produce and market value-added products. Major project activities include (1) a survey of farmstead and artisan cheesemakers and other on-farm processors in the Northeast to assess their business performance and needs for information and assistance, (2) a survey of 400-500 farmers who have participated in VIAC programs to identify obstacles to developing a value-added business, (3) a consumer survey and (4) a retailer survey to examine their preferences and attitude toward local cheese and other dairy products and to develop marketing strategies, and (5) development of education materials and outreach activities to enhance the programs of VIAC and Vermont extension. This project started in February 2010 and we are in the process of the developing the surveys.

Outcomes & Impacts:
We are in the process of collecting secondary data and developing surveys for collecting primary data.

Publications:
Wang, Q., R. Parsons and G. Zhang. 2010. China's dairy market: Trends, disparities and implications for trade. China Agricultural Economic Review 2(3): 356-371.


Hatch
Dairy Pharming: Production of Pharmaceutically Active Human Proteins in Bovine Milk .

Principal Investigator: F. Zhao

Accomplishments & Outputs:
The overall goal of this project is to develop innovative ways to generate transgenic cows which produce human insulin in milk at a minimum concentration of 1 mg/ml at a much reduced cost and with an improved success rate. In the first year, we isolated human genomic DNA from the human breast tissue and amplified approximately 1.5 kb human insulin genomic sequence that span whole insulin gene (introns and exons). The sequence of human insulin gene was cloned into the vector plasmid pBC1 (Invitrogen) which is specifically designed for generating transgenic animals to express transgene in milk and contains the goat beta-casein promoter and 5'and 3'-untranslated sequences. In the last year, we cloned the human insulin cDNA sequence into the pBC1. Both the pBC1/human insulin genomic sequence and the pBC1/human insulin cDNA sequence plasmid DNAs were transfected into the mouse mammary epithelial cell line HC11 cells and the cells were treated by the lactogenic hormones prolactin and glucocorticoids. However, the induction of insulin expression in HC11 cells was not observed in both transfections. Since the plasmids were over 20 kb, it is possible that the transfection efficiency of our expression plasmids into the HC11 were too low. To test the possibility, we have cloned the GFP sequence into our pBC1/human insulin cDNA plasmid either under the control of beta-casein promoter or under the independent control of pCMV promoter. We will use these plasmids to monitor and improve our transfection efficiency and test the hormonal induction of our constructs. When the induction is confirmed, the constructs will be tested in transgenic mouse models.

Outcomes & Impacts:
Human insulin gene and cDNA have been successfully obtained and their sequences have been verified by sequencing. These sequences have been cloned into the mammary gland expression vector pBC1 under control of goat beta-casein gene promoter and flanking sequences. These are the first steps for us to generate transgenic animals which produce high amount of human insulin in milk. Achievement of our aims will not only advance our current techniques of producing life-saving medicine with great impact on human well being, but also help to create new ways for sustainable agriculture.


National Institute of Food and Agriculture/Department of Agriculture
Properties and Regulation of Glucose Transporters in Bovine Mammary Gland .

Principal Investigator: F. Zhao

Accomplishments & Outputs:
The major activities during the reporting period were: 1) to determine the transport kenetics of bovine GLUT1 in Xenopus oocytes (aim 1) and 2) to investigate hormonal regulation of glucose transporter expression in bovine mammary gland tissue explants (aim 3). We have carried out mammary gland tissue biopsies in 7 cows and treated these tissues with IGF-1, insulin (I), I+hydrocortisone(H)+prolactin(P), or I+H+P+Estrogen(E) in tissue explants. We have partially analyzed the gene expression changes of glucose transporters in these explants. Two graduate students (one M.S. and one Ph. D.) have been trained in the project. Dissemination: Dr. Zhao and Yong Shao, a graduate student, have both presented research results of this project in the ADSA/ASAS/PAS Annual Meeting 2010 in Denver, Colorado. The data generated from the project have been presented in the seminars at Nanjing Agricultural University and Inner Mongolia Agricultural University of China and helped establishment of collaboration with Zhejiang University of China.

Outcomes & Impacts:
Bovine GLUT1 was expressed in Xenopus oocytes by microinjection of in vitro transcribed cRNA and was found to be localized to the plasma membrane, which resulted in increased glucose uptake. This bGLUT1-mediated glucose uptake was dramatically inhibited by cytochalasin B and phloretin. Kinetic analysis of bovine GLUT1 was conducted under zero-trans conditions using radio-labeled 2-deoxy-D-glucose and the principles of Michaelis-Menten kinetics. Bovine GLUT1 exhibited a KM of 7.69 +- 1.7 mM for 2-deoxy-Dglucose. Transport by GLUT1 was inhibited by mannose and galactose, but not fructose. Our data provides insight into the role of bovine GLUT1 in supplying the mammary gland with glucose during milk synthesis. Insulin-like growth factor-1 (IGF-1) is a potent mitogen for mammary epithelial cells and plays an important role in mammary development. To investigate the role of IGF-1 in regulating the expression of main glucose transporters GLUT1, GLUT8 and GLUT12 in bovine mammary epithelial cells, two experiments have been carried out. In the first experiment, Mac-T cells were treated for 12 hours by different doses of IGF-1 (20 ng/ml, 50 ng/ml, 100 ng/ml, 200 ng/ml and 400 ng/ml). mRNA levels of GLUT1, GLUT8, GLUT12 and IGFBP3 were determined by real-time PCR. IGFBP3 mRNA increased 6 to 13 fold in all groups treated with 50 ng/ml or higher concentrations of IGF-1 compared to non-treatment group (P<0.001), indicating that Mac-T cells is responsive to IGF-1. There were no treatment effects on GLUT1 and GLUT8 mRNA, but mRNA levels of GLUT12 decreased by 75 percent in all groups treated with 50 ng/ml or more IGF-1 relative to control group (P<0.001). Similarly, Mac-T cells treated with insulin (5 and 10 mg/ml) for 12 hours also showed more than 60% decrease in GLUT12 mRNA level (p<0.001). In the second experiment, Mac-T cells were treated with 100 ng/ml of IGF-I for 12 hours, 18 hours and 24 hours. Interestingly, mRNA of GLUT12 decreased to 30 percent after 12 hour treatment but returned to the same levels as in the non-treatment group after 24 hours.

Publications:
Shao Y, Zhao F-Q (2010) IGF-1 and insulin regulate the expression of GLUT12 in bovine mammary epithelial cells. J. Anim. Sci. Vol. 88, E-Suppl. 2/J. Dairy Sci. Vol. 93, E-Suppl. 1/Poult. Sci. Vol. 89, E-Suppl. 1, 809

Bentley PA, Misra Y, Morielli, AD, Zhao F-Q (2010) Characterization of bovine glucose transporter 1 kinetics and substrate specificities in Xenopus laevis oocytes. J. Anim. Sci. Vol. 88, E-Suppl. 2/J. Dairy Sci. Vol. 93, E-Suppl. 1/Poult. Sci. Vol. 89, E-Suppl. 1, 483


Hatch
Strategies to Limit Reductions in Maple Sap Yield in Vacuum Installations .

Principal Investigator: T. Perkins

Accomplishments & Outputs:
The goal of this project was to examine the impacts of aging and associated microbial contamination on sap yields in maple tubing systems under vacuum. Using replicated field plots, we compared new tubing systems versus tubing installations that were up to five years old. In addition, we explored methods to maintain high sap yields.

Outcomes & Impacts:
Tubing systems show gradual and continuing reductions in sap yield as they age. By the time they are five years old, they have lost up to one third of their total sap yield potential. Using annually-replaceable spout adapters or replacing drops (spouts, dropline and tees) can partially, but not completely offset these losses. Use of the Check-Valve spout adapter shows much greater promise in maintaining high sap yields. Maple syrup producers can apply these findings to achieve higher production, and thus a higher economic benefit.

Publications:
Perkins, T.D., B. Stowe, and T.R. Wilmot. 2010. Changes in sap yields from tubing systems under vacuum due to system aging. Maple Digest 22A(3): 20-27.


1b

Hatch
Potential impacts of global warming on autumn coloration in maple .

Principal Investigator: T. Perkins

Accomplishments & Outputs:
The annual display of autumn coloration in Vermont is a highly prized, economically and culturally important event. Global warming will result in a longer growing season and the temperature of both the autumnal days and nights will increase. Because of the strong relationship between environmental conditions and leaf senescence, these changes in climate have the potential to disrupt the normal physiological processes involved in the development of autumn coloration, particularly the development of red-colored anthocyanin pigments. And although there has been a great deal of speculation about possible impacts of global warming on fall color development, there has been little scientific study of the phenomenon. Thus, the overall objective of this project is to investigate the effects of temperature on the development of autumn colors in maple. The results obtained will help us to make more informed statements about the effects of global warming on autumn coloration. Several experiments are currently being conducted to investigate how temperature affects the onset of autumn coloration, and also to probe the physiological basis for any effects of temperature observed. The first experiment was focused on determining if cold temperatures promote the development of autumn coloration. In these experiments, red and sugar maple seedlings were subjected to various temperature treatments for one week prior to the onset of fall coloration, and their subsequent color development was monitored with nondestructive pigment content meters and digital photography. The results indicated that temperature does impact the development of fall coloration: colder temperatures promoted the onset of coloration, while warmer temperatures delayed the onset. Thus, the next set of experiments will focus on determining the physiological mechanisms through which cold temperatures promote autumn coloration. In these experiments, leaf petioles or portions of leaves will be cooled at night and during the day, and color development subsequently monitored throughout the fall. The results obtained will provide scientific data to help understand the potential effects of climate change on autumn coloration, and will help guide the design of subsequent experiments to be conducted for the project.

Outcomes & Impacts:
The experiments for this project are still ongoing, and thus no major results or findings have yet been obtained. The annual display of autumn leaf coloration in Vermont is a highly prized, economically and culturally important event. It is an integral part of the state's public image and cultural heritage, and is an economic engine that drives the generation of millions of dollars in revenue for the state from tourism and related activities each fall. There is a growing urgency to understand how global warming will affect this process and how these effects might impact the economic well-being of the state. Results from these experiments will provide a scientific basis for projecting the potential impact of future warm autumns on leaf coloration and will allow us to make more informed statements about the effects of global warming on autumn coloration.


North American Maple Syrup Council
Maximizing Vacuum Transfer and Sap Yields in Tubing Operations: Evaluation of a New Method to Increase Production .

Principal Investigator: T. Perkins

Accomplishments & Outputs:
This project seeks to determine whether reducing restrictions in maple sap collection tubing systems under vacuum will improve sap yields.

Outcomes & Impacts:
No differences in sap yield were observed between the control (industry standard) treatment and experimental (mainline to each tree) treatment. This may have been due to the scarcity of peak sap flow events during the 2010 season.


National Institute of Food and Agriculture/Department of Agriculture
Achieving High Maple Sap Yields .

Principal Investigator: T. Perkins

Accomplishments & Outputs:
This project sought to determine various methods of improving vacuum transfer in maple tubing systems to increase sap yields. A replicated large-scale field test was conducted at the University of Vermont Proctor Maple Research Center in Underhill Ctr., Vermont. Various treatments were installed to provide better transfer of vacuum to the taphole and sap yields were monitored throughout the 2010 maple season.

Outcomes & Impacts:
Relatively little differences among treatments were noted, probably due to the unusual character of the flow season, characterized by low-moderate sap flows with very little peak sap flow periods. Further examination over several successive years will be required to completely understand the effects of these treatments on sap yield.


Department of Agriculture USDA
Effects of Sap Preconcentration by Reverse Osmosis on Maple Syrup Chemical Composition and Flavor .

Principal Investigator: T. Perkins

Accomplishments & Outputs:
This project will compare the chemistry, quality, and flavor of maple syrup made with and without reverse osmosis. We will process raw maple sap and maple concentrate made from the same pool of sap simultaneously in two identical evaporators.

Outcomes & Impacts:
Maple producers will be informed as to how their selected style of sap processing affects the maple syrup product.


Hatch/Multistate
Multi-State Evaluation of Winegrape Cultivars and Clones .

Principal Investigator: L. Berkett

Accomplishments & Outputs:
Cold climate wine grape production is an emerging new crop in Vermont and the region offering exciting value-added and agri-tourism economic opportunities. A key challenge to this young industry is the selection of wine grape cultivars which will consistently produce high quality fruit under our variable environmental conditions. High quality fruit is the basis for quality wine production. This project is evaluating viticultural and IPM characteristics of new wine grape cultivars and will help to identify the most promising cultivars for Vermont and is part of a national research project (NE1020). This past growing season was the second year of grape production in the research vineyard containing eight wine grape varieties which was planted at the University of Vermont Horticultural Research Center. Observations and data were collected in the vineyard throughout the growing season and at harvest to determine differences between the varieties. Standard research protocols including those established by the NE1020 technical committee were used. Information was posted on the UVM Cold Climate Grape website. Currently, 2010 harvest data are being analyzed.

Outcomes & Impacts:
The UVM Vineyard is a research/demonstration site that has eight winegrape varieties. The winegrape varieties are part of a national evaluation of winegrape varieties (a joint USDA NE1020 Project with the Viticulture Consortium-East), an EPA Pesticide Environmental Stewardship Program Project, and a UVM Agric. Exp. Station Research Project. It was planted in 2007, using a randomized complete block experimental design of six blocks with four-vine plots of each winegrape variety per block. The vines are being trained to a high-wire cordon system; the soil is a well-drained Windsor loamy sand. In 2010, disease and arthropod data were collected at various times during the growing season. Research was presented at an international winegrape disease conference. Phenological stages were recorded for the different grape varieties and posted on the Cold Climate Grape website at http://pss.uvm.edu/grape/UVMvineyard/2010UVMphenology.html. Yield and juice data were collected at harvest for the eight wine grape varieties. An Open House and Tour of the Vineyard was conducted in 2010 and was attended by 115 people.

Publications:
Berkett, L.P., Bradshaw, T.L., . Kingsley-Richards, S.L., and M.L. Cromwell. 2010. Cold Climate Grape Production website. http://pss.uvm.edu/grape/

Berkett, L.P., T.L. Bradshaw, S.L. Kingsley-Richards, and M.L. Cromwell. 2010. Differences in incidence and severity of powdery Mildew and Down Mildew among Cold Climate Wine Grape Culivars and Table Grape Cultivars in 2009. Proceedings of the 6th International Workshop on Grapevine Downy and Powdery Mildew. Bordeaux, France. pp. 187-189.

Kingsley-Richards, S.L., Berkett, L.P., and T.L. Bradshaw. 2010. 2009 Key Phenological Dates for Eight Winegrape Cultivars. Cold Climate Grape Production website. http://pss.uvm.edu/grape/UVMvineyard/2009KeyPhenologyDatesWinegrapes.pdf

Berkett, L.P. 2010. Cold Climate Grape IPM Updates. 9 issues, 32 pp. http://pss.uvm.edu/grape/newsletters/.


Hatch/Multistate
IMPROVING ECONOMIC AND ENVIRONMENTAL SUSTAINABILITY IN TREE-FRUIT PRODUCTION THROUGH CHANGES IN ROOTSTOCK USE .

Principal Investigator: L. Berkett

Accomplishments & Outputs:
Apples are an important component of diversified agriculture in Vermont. It is a viable, competitive agricultural industry in the state. Although there has been a reduction in total hectares planted to apples over the last ten years, production has remained the same indicating an increase in efficiency and productivity. One of the reasons for the increase in productivity is the trend towards higher density plantings based on growers selection of dwarfing or semi-dwarfing rootstocks. Selection of newer rootstocks adapted to Vermonts climate and which will produce high quality fruit from high-yielding trees is a crucial decision for apple growers which will affect their competitiveness, profitability and sustainability for many years. Apple rootstock evaluation research has been described as essential to Vermonts apple industry. The objective of this research was to evaluate the field performance of apple rootstocks under Vermont conditions. Research was completed in the 2008 growing season.

Outcomes & Impacts:
Active research on this project was concluded during the 2008 growing season.


National Institute of Food and Agriculture/Department of Agriculture
Using New Alternatives to Enhance Adoption of Organic Apple Production through Integrated Research, Education, and Extension .

Principal Investigator: L. Berkett

Accomplishments & Outputs:
Apples are an important component of New England's diversified agriculture. Although there is significant interest in organic production, there are very few organic apple orchards in New England, in part, because of the challenges associated with the traditional apple cultivar grown (McIntosh). However, because of recent shifts in consumer preference for newer cultivars, growers are planting different apple cultivars. Growers want to know what the potential is for sustainable and profitable organic production with the newer apple cultivars that are being planted in the region. This project holistically examined the opportunities and challenges of organic production within the two major orchard systems growers are using to change to new cultivars and with five of the top apple cultivars that growers identified as important to the future of the industry. The project covered the establishment years of the two orchard systems; the project is being continued with another grant funded by the USDA Organic Research and Extension Initiative to study the early bearing years of the two organic orchards. The long-term goal of this multi-state, multidisciplinary project is to enhance adoption of organic apple production in New England through research that advances the scientific knowledge base and provides practical information to stakeholders through a closely integrated extension/outreach program. Outputs over the duration of the project included development of a organic apple production website (http://www.uvm.edu/organica/ ); development and offering an undergraduate course on organic fruit production; Organica Workshops and Orchard Tours; presentations at national and regional apple grower and/or research/extension meetings, presentations at international scientific conferences; answering grower questions on organic apple production; publishing Orchard Observations which was a web log of orchard observations during the growing season. A report on the activities, accomplishments and research insights of the project was published at: http://www.uvm.edu/~organica/OrganicAProject/2009ProgressReport/progress.html

Outcomes & Impacts:
All aspects of the OrganicA Project have received high praise from growers and the public. The project has increased knowledge of organic apple production and has created a change in action among program participants. A project evaluation by stakeholders is at: http://www.uvm.edu/~organica/OrganicAProject/2009ProgressReport/stakeholderevaluation.html The OrganicA Project has become a leading resource for organic information on the world wide web. Organic apple research results and insights have been presented at regional workshops involving growers, scientists, extension personnel, and agricultural consultants and at national and international scientific conferences. Although the orchards are young and are just beginning to produce a crop, the following are a few insights gained during the orchard establishment years: (i)Trees top-grafted onto established root systems showed varying degrees of success during the establishment years both in initial scion survival and extent of tree development. Growers who choose to top-graft an orchard rather than remove trees and plant a new orchard do so under the conventional wisdom that by-passing a two year fallow period followed by three establishment years for the new trees will give them a marketable crop sooner. The preliminary results indicate that poor survival of top-grafted trees on particular cultivars may negate the benefit of earlier production. (ii)Based on initial foliar disease assessments during the establishment years of the orchards, Honeycrisp appears more resistant to apple scab than the other scab-susceptible cultivars Zestar!, Ginger Gold, and Macoun, but appears more susceptible to rust, than Liberty and Zestar!. Honeycrisp and Liberty appear more attractive to Japanese beetles. Although beneficial insects are prevalent in the orchards, European red mite populations are increasing. The importance of a thorough assessment of the surrounding ecosystem for sources of inoculum and reservoirs of insect pests has been underscored in the two orchard systems being studied. (iii)Weed management in the newly-planted orchard has presented challenges. Various techniques have been implemented with limited success. Tree growth in one of the orchard systems has been sub-optimal with short terminal growth and trees not filling their allotted trellis space despite maintaining appropriate nutrient levels and consistent irrigation. We plan to use wood chip mulch during the next phase of this project and will monitor weeds and soil health plus test alternative nutrient sources. Also, an advanced study of weed management techniques will be conducted in a Maine orchard. (iv)To date we have compared the economics of the two orchard systems through calculating the Net Present Value (NPV) of cash inflows and outflows during the establishment period. To no surprise, neither system has produced a positive cash flow in any year since startup. This establishment period is a time when orchard plantings normally experience negative cash flows simply because it takes several years before any apples are available to generate revenue.

Publications:
Berkett, L. P. and T. L. Bradshaw. 2009. Organic Tree Fruit Production in New England. New England Tree Fruit Prod. Guide. Coop. Extension Systems of New England.

Berkett, L., Garcia, M., Moran, R., Darby, H., Parsons, R., Hayden, J., Bradshaw, T., Kingsley-Richards, S. and Cromwell, M. 2009. The OrganicA Project: Organic Disease Management in Orchards with Newer Cultivars. International Organization for Biological and Integrated Control Bulletin: 428-432.

Berkett, L.P. 2009. Organic Orchard Observations, 14 issues. OrganicA website.

Berkett, L.P., Moran, R., Garcia, M.E., Darby, H., Parsons, R., and J. Hayden. 2009 The OrganicA Project Progress Report: June 2006 - March 2009. The OrganicA Project Website. http://www.uvm.edu/~organica/OrganicAProject/2009ProgressReport/progress.html

Garcia, M. E., L. P. Berkett, T. Bradshaw, and S. Kingsley-Richards, R. E. Moran, and R. Parsons. 2009. Horticultural Options when Starting an Organic Apple Orchard. 16th SFOAM Organic World Congress. Modena, Italy. Submitted Dec. 2009. (Accepted.)


Environmental Protection Agency
Achieving Adoption of Ecologically-Based IPM and Reducing Pesticide Risks in Cold Climate Winegrape Production in Northern New England .

Principal Investigator: L. Berkett

Accomplishments & Outputs:
The cold climate winegrape industry is rapidly expanding; it is a new crop in the region that offers significant value-added and agri-tourism economic opportunities. The newly emerging industry is at a critical stage in establishing production practices that will impact pesticide use and exposure across northern New England. This project is the next step in achieving adoption of ecologically-based IPM and reducing pesticide risks within cold climate winegrape production. It will capture the momentum and success of the current regional cold climate winegrape IPM program and significantly move cold climate winegrape growers along the IPM Transition Gradient reducing exposure to pesticides that pose the greatest risk to human health and the environment. During the past year, the project involved developing resources on the UVM Cold Climate Grape website including IPM updates during the growing season, implementing IPM in a demonstration vineyard, one-on-one education, and an educational vineyard tour.

Outcomes & Impacts:
The specific objectives of the project are: 1. Survey the rapidly expanding community of cold climate winegrape growers as to their pesticide use practices and their knowledge and adoption of ecologically-based IPM and reduced-risk alternatives. This survey was conducted and the results provided baseline data to guide the educational outreach component of this project and will be used to evaluate the impact of the project. 2. Provide education and outreach to address gaps in the knowledge and adoption of reduced-risk IPM techniques and tools within the community of cold climate winegrape growers. The goal is to provide growers with the knowledge and tools to reduce pesticide risks and to increase adoption of ecologically-based IPM. Resources were developed and posted on the Cold Climate Grape website and a vineyard tour was conducted. 3. Investigate and demonstrate the application of reduced-risk IPM techniques and tools in a vineyard planted with representative cold climate winegrape varieties. This study continued in 2010; a vineyard tour was conducted during the growing season wherein IPM techniques used in the vineyard were discussed with growers and industry representatives.

Publications:
Berkett, L.P. 2010. 2009 Vermont Cold Climate Grape Survey Results. 41pp. http://pss.uvm.edu/grape/2009ColdClimateGrapeSurveyResults.pdf

Berkett, L.P. 2010. Cold Climate Grape Production. Website. http://pss.uvm.edu/grape/

Berkett, L.P. 2010. Cold Climate Grape IPM Updates.9 issues, 32 pp. http://pss.uvm.edu/grape/newsletters/


Cornell University
Coordinated Wine Grape Variety Evaluations in the Eastern USA .

Principal Investigator: L. Berkett

Accomplishments & Outputs:
Cold climate wine grape production is an emerging new crop in Vermont and the region offering exciting value-added and agri-tourism economic opportunities. A key challenge to this young industry is the selection of wine grape cultivars which will consistently produce high quality fruit under our variable environmental conditions. High quality fruit is the basis for quality wine production. This project is evaluating viticultural and IPM characteristics of new wine grape cultivars and will help to identify the most promising cultivars for Vermont. In 2010, the second year of grapes production was harvested in the research vineyard containing eight wine grape varieties which was planted at the University of Vermont Horticultural Research Center. Observations and data were collected in the vineyard throughout the growing season and at harvest to determine differences between the varieties. Information was posted on the UVM Cold Climate Grape website. Currently, 2010 harvest data are being analyzed.

Outcomes & Impacts:
The UVM Vineyard is a research/demonstration site that has eight winegrape varieties. The winegrape varieties are part of a national evaluation of winegrape varieties (a joint USDA NE1020 Project with the Viticulture Consortium-East), an EPA Pesticide Environmental Stewardship Program Project, and a UVM Agric. Exp. Station Research Project. It was planted in 2007, using a randomized complete block experimental design of six blocks with four-vine plots of each winegrape variety per block. The vines are being trained to a high-wire cordon system. The soil is a well-drained Windsor loamy sand; drip irrigation has been installed. In 2010, disease and arthropod data were collected at various times during the growing season. Phenological stages were also recorded for the different grape varieties and posted on the Cold Climate Grape website at http://pss.uvm.edu/grape/UVMvineyard/2010UVMphenology.html. Yield and juice data were collected at harvest for the eight wine grape varieties. An Open House and Tour of the UVM Vineyard was conducted in 2010 and was attended by 115 people.


Vermont Agency of Agriculture, Food, and Markets
Specialty Crops - Apple and Grape IPM .

Principal Investigator: L. Berkett

Accomplishments & Outputs:
Apples and grapes are important in the diversification of agriculture in Vermont and are important specialty crops. This grant from the Vermont Department of Agriculture helps to support the collection of orchard and vineyard IPM data and information for inclusion into apple and grape newsletters and websites; acquisition and inclusion of weather data for apple and grape newsletters and websites; development and electronic publication of apple and grape newsletters; development and implementation of an apple and grape industry education and training workshops.

Outcomes & Impacts:
The grant supported the collection of orchard and vineyard IPM data and information for inclusion into apple and grape newsletters and websites; acquisition and inclusion of weather data for apple and grape newsletters and websites; development and electronic publication of apple and grape newsletters; development and implementation of an apple and grape industry education and training workshops. Information was sent to growers throughout the growing season and posted on the UVM Apple website and the UVM Cold Climate Grape website.

Publications:
Berkett, L.P. 2010. Cold Climate Grape IPM Updates. 9 issues, 32 pp. http://pss.uvm.edu/grape/newsletters/

Berkett, L.P. 2010. Vermont Apple IPM Alerts. 17 issues, 60 pp. http://orchard.uvm.edu/uvmapple/pest/index.html

Berkett, L.P. 2010. The Cold Climate Grape Production website: http://pss.uvm.edu/grape/

Berkett, L.P. 2010. UVM Apple Orchard website: http://orchard.uvm.edu/


National Institute of Food and Agriculture/Department of Agriculture
Using ""New"" Alternatives to Enhance Adoption of Organic Apple Production Through Integrated Research and Extension .

Principal Investigator: L. Berkett

Accomplishments & Outputs:
Apples are an important component of diversified agriculture in Vermont and New England. Although there is significant interest in organic production, there are very few organic apple orchards in New England, in part, because of the challenges associated with the traditional apple cultivar grown (McIntosh). However, because of recent shifts in consumer preference for newer cultivars, growers are planting different apple cultivars. Growers want to know what the potential is for sustainable and profitable organic production with the newer apple cultivars that are being planted in the region. This project holistically examines the opportunities and challenges of organic production within the two major orchard systems growers are using to change to new cultivars and with five of the top apple cultivars that growers identified as important to the future of the industry. The long-term goal of this multi-state, transdisciplinary project is to enhance adoption of organic apple production in Vermont and New England through research that advances the scientific knowledge base and provides practical information to stakeholders. Through a closely integrated extension/outreach program that addresses stakeholder needs, the project will enable whole farm planning, improve competitiveness, and enhance the ability of growers to grow and market high quality organic apples. Outputs during this past year included further development of the OrganicA website (http://www.uvm.edu/organica/ ); an Organica Workshop/Tour; presentations at regional apple grower and/or research/extension meetings, answering grower questions on organic apple production; and publishing 12 issues of Orchard Observations which is a web log of orchard observations.

Outcomes & Impacts:
This project holistically is examining the opportunities and challenges of organic apple production within the two major orchard systems growers are using to change to new cultivars and with five of the top apple cultivars that growers identified as important to the future of the industry. The project was initiated in 2006 and since then, all aspects of the OrganicA Project have received high praise. The project has increased knowledge of organic apple production and has created a change in action among program participants. The OrganicA Project has become a leading resource for organic information on the world wide web. Research results and insights have been presented at regional workshops involving growers, scientists, extension personnel, and agricultural consultants and at national and international scientific conferences. This long-term project was nationally ranked #1 by the USDA Organic Agriculture Research & Extension Program upon review for funding the second phase of this long-term project.

Publications:
Berkett, L. P. and T. L. Bradshaw. 2009. Organic Tree Fruit Production in New England. New England Tree Fruit Prod. Guide. Coop. Extension Systems of New England.

Berkett, L., Garcia, M., Moran, R., Darby, H., Parsons, R., Hayden, J., Bradshaw, T., Kingsley-Richards, S. and Cromwell, M. 2009. The OrganicA Project: Organic Disease Management in Orchards with Newer Cultivars. International Organization for Biological and Integrated Control Bulletin: 428-432.

Cromwell, M. and L. Berkett. 2009. Summary of an Evaluation of Alternative Organic Fungicides for Apple Scab Management in Vermont. The OrganicA Project website. http://www.uvm.edu/organica/OrganicOrchardInformation/OrganicIPM/

Cromwell, M. and L. Berkett. 2009. Summary of a Preliminary Evaluation of Raw Milk as a Fungicide for Apple Scab Management in Vermont. The OrganicA Project website. http://www.uvm.edu/organica/OrganicOrchardInformation/OrganicIPM/ Berkett, L.P., Moran, R., Garcia, M.E., Darby, H., Parsons, R., and J. Hayden. 2009 The OrganicA Project Progress Report. The OrganicA Project Website. http://www.uvm.edu/organica/OrganicAProject/2009ProgressReport/progress.html

Berkett, L., Moran, R., Garcia, E., Darby, H., Parsons, R., Hayden, J., Bradshaw, T., Kingsley-Richards, S. and M. Cromwell. 2009. USDA/National Organic Program: New England Tree Fruit Orchard Research and Demonstration Plot Update. Great Lakes Fruit, Vegetable and Farm Market EXPO, Grand Rapids, MI.

Garcia, M. E., L. P. Berkett, T. Bradshaw, and S. Kingsley-Richards, R. E. Moran, and R. Parsons. 2010. Horticultural Options when Starting an Organic Apple Orchard. Acta Hort. 873. ISHS. 277-281.

Berkett, L.P. 2010. Organic Orchard Observations, 12 issues, 52 pp. http://www.uvm.edu/organica/ListservesBlogs/listservesblogs.html

Berkett, L., Moran, R., Garcia, E., Darby, H., Parsons, R., Hayden, J., Bradshaw, T., Kingsley-Richards, S. and M. Cromwell. 2010. The OrganicA Project website: http://www.uvm.edu/organica/

Garcia, E.M., Berkett, L.P., Moran E R., Bradshaw, T., Kingsley-Richards, S. 2010. Top-Grafting: A Viable Alternative when Changing Cultivars in An Apple Orchard? 28th International Horticultural Congress, Lisbon-Portugal, 22-27 August 2010.


1d

Cornell University
Coordinated Wine Grape Variety Evaluations in the Eastern USA .

Principal Investigator: L. Berkett

Accomplishments & Outputs:
Cold climate wine grape production is an emerging new crop in Vermont and the region offering exciting value-added and agri-tourism economic opportunities. A key challenge to this young industry is the selection of wine grape cultivars which will consistently produce high quality fruit under our variable environmental conditions. High quality fruit is the basis for quality wine production. This project is evaluating viticultural and IPM characteristics of new wine grape cultivars and will help to identify the most promising cultivars for Vermont. In 2010, the second year of grapes production was harvested in the research vineyard containing eight wine grape varieties which was planted at the University of Vermont Horticultural Research Center. Observations and data were collected in the vineyard throughout the growing season and at harvest to determine differences between the varieties. Information was posted on the UVM Cold Climate Grape website. Currently, 2010 harvest data are being analyzed.

Outcomes & Impacts:
The UVM Vineyard is a research/demonstration site that has eight winegrape varieties. The winegrape varieties are part of a national evaluation of winegrape varieties (a joint USDA NE1020 Project with the Viticulture Consortium-East), an EPA Pesticide Environmental Stewardship Program Project, and a UVM Agric. Exp. Station Research Project. It was planted in 2007, using a randomized complete block experimental design of six blocks with four-vine plots of each winegrape variety per block. The vines are being trained to a high-wire cordon system. The soil is a well-drained Windsor loamy sand; drip irrigation has been installed. In 2010, disease and arthropod data were collected at various times during the growing season. Phenological stages were also recorded for the different grape varieties and posted on the Cold Climate Grape website at http://pss.uvm.edu/grape/UVMvineyard/2010UVMphenology.html. Yield and juice data were collected at harvest for the eight wine grape varieties. An Open House and Tour of the Vineyard was conducted in 2010 and was attended by 115 people.


1d

Vermont Agency of Agriculture, Food, and Markets
Specialty Crop Block Grant Program Funds for Apple and Grape Program .

Principal Investigator: L. Berkett

Accomplishments & Outputs:
Apples and grapes are important in the diversification of agriculture in Vermont and are important specialty crops. This grant from the Vermont Department of Agriculture helps to support the collection of orchard and vineyard IPM data and information for inclusion into apple and grape newsletters and websites; acquisition and inclusion of weather data for apple and grape newsletters and websites; development and electronic publication of apple and grape newsletters; development and implementation of an apple and grape industry education and training workshops.

Outcomes & Impacts:
The grant supported the collection of orchard and vineyard IPM data and information for inclusion into apple and grape newsletters and websites; acquisition and inclusion of weather data for apple and grape newsletters and websites; development and electronic publication of apple and grape newsletters; development and implementation of an apple and grape industry education and training workshops. Information was sent to growers throughout the growing season and posted on the UVM Apple website and the UVM Cold Climate Grape website.

Publications:
Berkett, L.P. 2010. Cold Climate Grape IPM Updates. 9 issues, 32 pp. http://pss.uvm.edu/grape/newsletters/

Berkett, L.P. 2010. Vermont Apple IPM Alerts. 17 issues, 60 pp. http://orchard.uvm.edu/uvmapple/pest/index.html

Berkett, L.P. 2010. The Cold Climate Grape Production website: http://pss.uvm.edu/grape/

Berkett, L.P. 2010. UVM Apple Orchard website: http://orchard.uvm.edu/


National Institute of Food and Agriculture/Department of Agriculture
A Public-Private Partnership to Promote IPM Implementation in Northern New England Greenhouse Ornamentals .

Principal Investigator: M. Skinner

Accomplishments & Outputs:
The greenhouse industry is vital to the survival of agriculture and small family farms in northern New England and the Northeast. New pest and disease problems are surfacing and the agrochemicals available to combat them, while more expensive, are in some cases, less effective. Environmental and human health hazards associated with extensive reliance on chemical pesticides are recognized, yet the efficacy of biological control options and their cost are key considerations. The fact that growers are faced with new pest management and production issues that affect the economy, health, expansion and sustainability of their industry must be supported by research and outreach activities to address these new issues. The goal of this planning grant is to identify the needs and crucial priorities of growers, the end users, by a thorough assessment of the current state of the industry and the identification of those areas where research and education can provide viable solutions to support growers. Based on the results from stakeholder surveys, a comprehensive full proposal will be prepared for submission to the USDA Specialty Crops Program. Several methods were used to survey stakeholders. Grower workshops were held in ME, NH and VT at which a grower-to-grower discussion session was held to learn directly what grower priorities were. In addition a questionnaire was sent out to over 3,000 growers in the area to solicit stakeholder opinions. A partnership conference was held to finalize priorities and establish objectives for the proposal. Results from the survey were presented at a recent conference and other grower workshops. In addition a summary of grower priorities will be placed on the UVM web site and the NE IPM Center site.

Outcomes & Impacts:
Results from the survey are currently being analyzed. However, one key finding to date is that a lack of knowledge about IPM is a critical factor limiting greater adoption of this environmentally friendly approach to production. Through this project we will identify what growers need to increase their implementation of IPM and enhance their economic stability. Based on results from this project, a realistic trans- and multi-disciplinary approach to address important IPM issues through research and outreach was developed and submitted to the Specialty Crops Research Initiative Program in 2010. Growers in no. New England were the first beneficiaries of this project as it could lead to activities that support their efforts to achieve effective pest management with as few chemical pesticides as necessary. Ultimately all of society will benefit from this project as it will lead to lower chemical pesticide use, and thus less exposure to toxic chemicals. The proposal was not selected for funding but plans are underway to revise and resubmit it.


International Society of Arboriculture
National Elm Tree Evaluation .

Principal Investigator: M. Starrett

Accomplishments & Outputs:
This research project is cooperative between 15 different sites/regions of the United States with the University of Vermont representing the New England region. The study is evaluating 19 Dutch elm disease resistant elm species/hybrids for: resistance to other diseases and insects, geographic adaptation, meteorological tolerance, growth rate, form and ornamental features, over a 10 year period. A poster, summarizing the results to date, was presented at the Vermont Division of Forestry's Urban and Community Forestry workshop, "Return of the American Elm to the Community Forest" held at Marsh-Billings-Rockefeller National Historic Park, Woodstock, VT on September 24, 2010.

Outcomes & Impacts:
This 10-year evaluation of the American and Asian hybrid elms is half-way completed and thus still too early to make any conclusive statements regarding plant performance. Preliminary results indicate that Ulmus x 'Frontier' (U. carpinifolia x U. parvifolia) has consistently developed an ornamental purple fall color. Also of note is the fact that U. parvifolia 'Emer II' (Allee elm) all died after the first winter in Vermont. These trees were replaced in 2007 with Ulmus parvifolia 'Emer I' which also has performed very poorly, with consistent winter injury to the branches and trunks. This damage indicates that while the parent, U. parvifolia can impart a reasonable fall foliage color in hybrid elms, the species itself may not be cold-hardy in Vermont (USDA Zone 5). In 2008, at the University of Vermont, two of the five specimens of the hybrid elm (Ulmus japonica x Ulmus wilsoniana) = Ulmus x 'Morton Red Tip' (Danada Charm), died from wetwood disease. In 2009, Ulmus x 'Pioneer' (Ulmus glabra x U. carpinifolia) died from stem canker. One of the native American elm selections in the trial (Ulmus americana 'Valley Forge'), consistently had problems with windthrow and had to be staked to maintain a straight trunk. The least ornamental and most disease-prone tree in the study is Ulmus x 'Homestead' (Ulmus glabra x U. carpinifolia x U. pumila). This tree has yet to develop a uniform crown and the foliage is consistently afflicted with anthracnose disease. Therefore, Ulmus x 'Homestead' is not recommended for use in residential or urban landscapes in Vermont. It is important to know which of American elm selections or hybrid Asian elms included in this research trial are suitable for use in public, residential and/or commercial landscapes. The most disease and pest resistant of these elms may be suitable replacements for ash trees currently being devastated by the Emerald Ash Borer (EAB) in the Great Lakes/Northern mid-west region and which is spreading eastward.


Stanley Smith Horticulture Trust
Stanley Smith Horticultural Trust Grant Proposal .

Principal Investigator: M. Starrett

Accomplishments & Outputs:
The goal of this project is to showcase the existing ornamental gardens, functional gardens (rain gardens and edible gardens) on the main campus at The University of Vermont. Creation of a website (http://www.uvm.edu/thegardens/) is underway which will detail specific gardens and their locations on campus so that faculty, staff, and students, as well as the general public can visit them either in person or remotely, via the web. To date, the website has been established and images of "The Gardens" have started to be uploaded to it. The plants in these gardens have been inventoried and put into an Excel file for future incorporation into a web-searchable/indexed database. The website URL will be part of the "A-to-Z" guide at The University of Vermont, which is found on the University's Homepage. Additionally, informational brochures containing "The Gardens" website URL will be available at key areas on campus (Davis Student Center, Admissions, and Waterman Administration building). Future activities will include continued development of the website with seasonal photos of the gardens and featured plants within each. Sources of internal and external funding will be identified which will help sustain the physical gardens through their maintenance, as well as the virtual interface with "The Gardens" through further website development.

Outcomes & Impacts:
The Gardens at The University of Vermont are a valuable resource for faculty, staff, students and the public but are not well known. This website will help residents and visitors to easily locate gardens on campus, to find out more about the plants each contains, and learn about their usefulness to the aesthetic value in the surrounding environment and/or their functional use as an edible plant or as environmental "modifier".