Safety Manual and Standard Operating Procedures
UVM's Biosafety Level 3 and Animal Biosafety Level 3 facilities
January 2022
SECTION 1: RESPONSIBILITIES
1.1 PRINCIPAL INVESTIGATOR
1.2 RESEARCH PERSONNEL
1.3 UVM BIOSAFETY OFFICE
1.4 UVM BIOLOGICAL SAFETY COMMITTEE (IBC)
1.5 UVM COLLEGE OF MEDICINE & VERMONT DEPARTMENT OF HEALTH (VDH)
1.6 OFFICE OF ANIMAL CARE MANAGEMENT (OACM)
SECTION 2: MEDICAL SURVEILLANCE PROGRAM
2.1 MEDICAL SURVEILLANCE
2.2 IMMUNIZATIONS
2.3 MEDICAL RESTRICTIONS
2.4 POST-EXPOSURE EVALUATION AND FOLLOW-UP
SECTION 3: LABORATORY PROCEDURES
3.1 ENTRY/EXIT PROCEDURES
3.2 PPE DONNING/DOFFING PROCEDURES
3.3 WORK PRACTICES
3.4 GENERAL A/BSL-3 LABORATORY PRACTICES & PROCEDURES
3.5 [vacated]
3.6 PERSONAL PROTECTIVE EQUIPMENT (PPE)
3.7 HANDWASHING
3.8 PROCEDURES FOR CENTRIFUGATION
3.9 BIOLOGICAL SAFETY CABINET (BSC) PROTOCOL
3.10 WASTE HANDLING AND DISPOSAL PROCEDURES
3.11 EQUIPMENT REPAIRS, SERVICE, OR DISPOSAL
3.12 SEMI-ANNUAL SHUTDOWNS
3.13 PROCEDURE FOR LAUNDERING LABORATORY CLOTHING (SCRUBS)
3.14 TRANSPORT OF POTENTIALLY INFECTIOUS MATERIALS BETWEEN A/BSL-3 LABS
3.15 TRANSPORT OF INACTIVATED MATERIALS TO A/BSL-2 LABS
3.16 INACTIVATION OF BIOLOGICAL SAMPLES
SECTION 4: SPILL RESPONSE PROCEDURES
GENERAL
4.1 SPILLS IN BSC
4.2 SPILLS IN THE LABORATORY
4.3 COMPOSITION OF A/BSL-3 SPILL KIT
4.4 SPILLS IN INCUBATOR
4.5 SPILLS IN CENTRIFUGE
4.6 CHEMICAL SPILL PROCEDURES
4.7 DECONTAMINATION OF AN A/BSL-3 SPILL
SECTION 5: EMERGENCY RESPONSE PROCEDURES
5.1 EMERGENCY PROCEDURES FOR EXPOSURE INCIDENTS
5.2 EMERGENCY PROCEDURES FOR PERSON DOWN/MEDICAL EMERGENCY (NO SPILL INVOLVED)
5.3 EMERGENCY PROCEDURES FOR MEDICAL EMERGENCY INVOLVING A BIOLOGICAL SPILL
5.4 AFTER HOURS EMERGENCIES
5.5 PROCEDURES FOR FIRE ALARM, HVAC FAILURE, BSC FAILURE, OR POWER LOSS
SECTION 6: MAINTENANCE PERSONNEL AND VISITOR PROCEDURES
6.1 VACCINATION REQUIREMENTS
6.2 PROCEDURES FOR ENTERING A/BSL-3 FACILITY
6.3 PERSONAL PROTECTIVE EQUIPMENT (PPE)
6.4 RESPONSIBILITIES OF THE A/BSL-3 FACILITY USERS
6.5 RESPONSIBILITIES OF BIOSAFETY STAFF
6.6 RESPONSIBILITIES OF MAINTENANCE PERSONNEL AND VISITORS
SECTION 7: REGISTERING YOUR WORK
7.1 REGISTRATION OF BIOLOGICAL MATERIALS
7.2 REGISTRATION OF RECOMBINANT DNA
7.3 REQUIREMENTS FOR BSL-3 EXPERIMENTATION IN ANIMALS
7.4 RISK ASSESSMENT
SECTION 8: AUTOCLAVE OPERATION
8.1 A/BSL-3 AUTOCLAVE USE
8.2 AUTOCLAVE VALIDATION
SECTION 9: A/BSL-3 PERSONNEL TRAINING
9.1 A/BSL-3 PERSONNEL TRAINING REQUIREMENTS
9.2 MEMORANDUM OF UNDERSTANDING (MOU) AND AGREEMENT FOR PI
9.3 A/BSL-3 USERS MEMORANDUM OF UNDERSTANDING (MOU) AND AGREEMENT
SECTION 10: FORMS
10.1 A/BSL-3 PERSONNEL TRAINING RECORD
10.2 STANDARD OPERATING PROCEDURES (SOP) TEMPLATE FOR A/BSL-3 RESEARCH LABORATORIES
10.3 A/BSL-3 VISITOR CLEARANCE FORM
10.4 RESEARCHER EXPERIENCE FORM
SECTION 11: A/BSL-3 CHECKLISTS
11.1 A/BSL-3 LABORATORY INSPECTION CHECKLIST
11.2 A/BSL-3 FACILITY CHECKLIST
11.3 CHECKLIST FOR MAINTENANCE WORK IN BSL-3 FACILITIES
11.4 A/BSL-3 OBSERVATION CHECKLIST
11.5 SEMI-ANNUAL A/BSL-3 SHUTDOWN CHECKLIST
SECTION 12: GUIDELINES AND REGULATIONS
12.1 CDC/NIH LABORATORY BIOSAFETY LEVEL CRITERIA - BIOSAFETY LEVEL 3
12.2 CDC/NIH VERTEBRATE ANIMAL BIOSAFETY LEVEL CRITERIA - ANIMAL BIOSAFETY LEVEL 3
12.3 CLASSIFICATION OF HUMAN ETIOLOGIC AGENTS