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Biomolecular Indicators of Acid Stress: Indicators of Acid Stress in Northeastern Forest Soil Methane Oxidizing Communities

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dataset_eml_metadataproviderheader

  • Forest Ecosystem Monitoring Cooperative

    • Address:
      705 Spear Street
      South Burlington, Vermont 05403
      United States of America

      Phone: (802) 391-4135
      Email: femc@uvm.edu
      Website: www.uvm.edu/femc

dataset_eml_abstractheader

    Investigate the effects of air quality, especially acid deposition, on terrestrial and aquatic ecosystems, and particularly on microbial communities.Study of the application of molecular biology techniques to investigate diversity, community structure, and function of methane oxidizing bacteria and nitrifying bacteria in forest soils affected by atmospheric acid deposition.

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  • Mark Bremer: dataset_eml_peopleprincipleinvestigator

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  • acid deposition, biomolecular, soil bacteria, soil chemistry, water chemistry,

Organizations

  • Syracuse University : lead

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  • dataset_eml_locationcords

    dataset_eml_datatableheader

    • dataset_eml_datatabletitle: Indicators of Acid Stress in Northeastern Forest Soil Methane Oxidizing Communities
    • dataset_eml_datatablestartdate: 2001-01-01
    • dataset_eml_datatableendate: 2002-01-01
    • dataset_eml_datatabledescription: Investigate the effects of air quality, especially acid deposition, on microbial ecosystems.

    • dataset_eml_datatablepurpose: Specific objectives include assessing the diversity and community structure of soil bacteria (nitrifiers and methanotrophs) in different acid depostion environments; develop a biomolecular method for determining ecosystem acid stress; and validate the PnET-BGC forest-soil-water model on field, GIS and remote sensing data then regionally apply the model.

    • dateset_eml_datatableshortname:

    • dataset_eml_datatablephysicalobjectname: VMC.154.1154

    • dataset_eml_datatabledatatype: mySQL
    • dataset_eml_datatablecitation: Mark Bremer. 2003. Indicators of Acid Stress in Northeastern Forest Soil Methane Oxidizing Communities. FEMC. Thesis, https://www.uvm.edu/femc/data/archive/project/biomolecular-indicators-acid-stress/dataset/Indicators-of-Acid-Stress-in-Northeastern-Forest-Soil-Methane-Oxidizing-Communities

    • dataset_eml_datatableonlinedistribution: https://www.uvm.edu/femc/CI4/data/archive/project/biomolecular-indicators-acid-stress/dataset/Indicators-of-Acid-Stress-in-Northeastern-Forest-Soil-Methane-Oxidizing-Communities

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    dataset_eml_methodsheader

    • dataset_eml_methodsPast (dataset_eml_notInUse)
      • Site establishment and sample collection
        • dataset_eml_methodsStartDate: 2002-06-10
          dataset_eml_methodsEndDate: 2002-06-11
        • dataset_eml_methoddescription: Establish 1 soil pit for every 100m elevation change. Mark and GPS each site; dig to mineral layer; collect 100 g of each horizon (O-B) and pool w/equivalent horizons. Collect 2 250 mL of stream H2O every 200m along a stream. Extract DNA from soil samples, use diagnostic genes to identify nitrifiers and methanotrophs, DNA fingerprint portions of those genes.

    dataset_eml_samplingheader

    • Soil
      • dataset_eml_methodsStartDate: 2002-06-10
        dataset_eml_methodsEndDate: 2002-12-31
      • dataset_eml_samplingdescription: Soil sampling
      • dataset_eml_samplingFrequency: 75 samples collected 50 g each at 3 soil horizons; collected at 3 elevations/site for each site

    • Water Samples
      • dataset_eml_methodsStartDate: 2002-06-10
        dataset_eml_methodsEndDate: 2002-12-31
      • dataset_eml_samplingdescription: Samples for water chemistry
      • dataset_eml_samplingFrequency: 80 samples collected; 500 mL at 5 elevations per site or about every 200 m

    dataset_eml_sitecharacteristicsheader

    • dataset_eml_sitecharacteristicsdescription: Hubbard Brook Experimental Forest, NH

    • dataset_eml_siteCharacteristicsMinAlt: 550 meters

    • dataset_eml_siteCharacteristicsMaxAlt: 790 meters

    • dataset_eml_sitecharacteristicsdescription: Lye Brook Wilderness Area, VT

    • dataset_eml_siteCharacteristicsMinAlt: 580 meters

    • dataset_eml_siteCharacteristicsMaxAlt: 760 meters

    • dataset_eml_sitecharacteristicsdescription: Willowemoc Creek, NY

    • dataset_eml_siteCharacteristicsMinAlt: 520 meters

    • dataset_eml_siteCharacteristicsMaxAlt: 700 meters

    • dataset_eml_sitecharacteristicsdescription: Bear Brook Watershed, ME

    • dataset_eml_siteCharacteristicsMinAlt: 265 meters

    • dataset_eml_siteCharacteristicsMaxAlt: 475 meters