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Found 354 Core Facilities


Clinical Pharmacology Dartmouth Medical School 1 Medical Center Drive Lebanon, NH 03756 (603) 650-7649 Bernard.B.Beaulieu.Jr@Hitchcock.ORG View Website Primary Contact: Bernard Beaulieu (603) 650-7649 Bernard.B.Beaulieu.Jr@Hitchcock.ORG Last Updated: 02/08/2012	Services offered: Clinical Assessment (Hematology, Clinical Chemistries, etc.) Mass Spectrometry Associations: Cancer Center
The Clinical Pharmacology Shared Resource operates in the Borwell Research Building at DHMC. In addition, to the biorepository function of our resource we also have bioanalytical hardware and computer hardware and state-of-the-art commercial software programs for application to individual and population-based pharmacokinetic and pharmacodynamic modeling, and modeling drug in vitro and in vivo combination drug effects.

Biological Imaging Facility - BIF Northwestern University 2205 Tech Drive Hogan Bldg Rooms 5-110, 5-120, 5-150 Evanston, IL 60208 (847) 467-0374 j-bufkin@northwestern.edu View Website Primary Contact: William Russin (847) 491-6657 w-russin@northwestern.edu Last Updated: 02/07/2012	Services offered: Cell Imaging Confocal Microscopy Electron Microscopy Microscopy Multiphoton Microscopy Optical Imaging Analytical, Computer Hardware/Software, Confocal Microscopy, Cryo/Low-Temp Preparation, Electron Microscopy, Fluorescence/Photonic Microscopy, Image Analysis, Imaging, Imaging Software, Input/Output, Light Microscopy, Sample Preparation, Specimen Pre Associations: MWACD
The Biological Imaging Facility is a shared-use research and training resource available to all NU researchers. BIF is organized so users can prepare samples, capture and analyze images, and create final presentations in one facility. Training for all instruments is available on a regular basis so users can acquire data quickly and efficiently. We are continuously looking for new ways to enhance existing equipment, acquire new tools, and keep pace with current techniques. ---- Adobe Creative Suite - Photoshop , Illustrator , InDesign , Acrobat, Cressington 626 Cryotransfer System, Cressington Quick Freeze Deep Etch - QFDE, Cressington Sputter Coater, Cryoultramicrotomy, Denton Vacuum Evaporator, FEI Vitrobot - plunge-freezing, FRAP, FRET, Fluorescence Correlation Spectroscopy - FCS, HP DesignJet 5500 PS large format printer for posters, ImageJ , MetaMorph , ImageProPlus, JEOL 1230 120 kV Transmission Electron Microscope - TEM, cryoTEM, Leica Automatic Freeze Substitution - FS, Leica High Pressure Freezer - HPF, Leica Laser Scanning Confocal Microscope - LSCM, Leica MM80 - slam-freezing, Leica Ultracut S Ultramicrotome - with FCS cryoultramicrotomy module, Leica Widefield Fluorescence Microscope, Networked Mac or PC computer workstations with CD/DVD drive , USB drive , Firewire drive, Pelco BioWave Microwave, Polaron Critical Point Dryer, RMC 6000 Ultramicrotome, RMC PT-PC Ultramicrotome, Training on BIF instruments, Umax PowerLook 2100 XL flatbed scanner, Volocity 5.X, a cross-platform image analysis program, Zeiss 510 Meta/ConfoCor3 Laser Scanning Confocal Microscope - LSCM, FCS

Protein Production Core Facility University of Delaware Delaware Biotechnology Institute 15 Innovation Way Newark, DE 19711 (302) 831-4687 wu@dbi.udel.edu View Website Primary Contact: Yu-Sung Wu (302) 831-3441 wu@dbi.udel.edu Last Updated: 01/18/2012	Services offered: Protein Extraction/Purification SDS-PAGE Support Services - Shared Instrumentation Oversight & Maintenance Western Blot Protein purification Associations: COBRE
The Protein Production Core (PPC) Facility is a core laboratory that serves academic investigators in Delaware. The PPC is part of shared core facilities supported by the Center of Biomedical Research Excellence (COBRE) in Membrane Protein Production and Characterization at the University of Delaware. The PPC has modern instrumentation for large scale expression, purification and characterization of prokaryotic and eukaryotic proteins for investigators and researchers. The core supports the production of proteins in quantities suitable for structural studies (X-ray and NMR) and functional studies (biological or catalytic). The PPC also has state-of-the-art analytical ultracentrifuge for studying macromolecule interactions. ---- fermentation

Monoclonal Antibody Core Facility Memorial Sloan Kettering Cancer Center 1275 York Avenue Box 509 New York City, NY 10065 (646) 888-2331 macf@mskcc.org View Website Primary Contact: Frances Weis-Garcia, Ph.D. (646) 888-2354 weisgarf@mkscc.org Last Updated: 01/04/2012	Services offered: Monoclonal Antibody Mycoplamsa Testing Associations: Cancer Center ABRF
The Monoclonal Antibody Core Facility serves scientists and clinicians by lowering the "experimental activation energy" of: 1.) Creating new MAbs through any combination of these services: ... Consultation ... Immunization of mice, rats or Armenian hamsters ... Fusion (B cell immortalization and selection for hybridomas) ... Maintenance of plated fusion while you screen for the functionality you seek ... ELISA screening ... Freezing positive cultures ... Cloning or subcloning ... Freeze spleen from immunized animals for future fusions 2.) Obtaining MAbs from established hybridomas as: ... Bioreactor Supernatants: ...... MAb concentrations range from 0.5 to 4.0 mg/ml (average ~ 1 mg/ml) ...... Purity ranges from 20 to 80% ...... Available aliquot size is usually 10 mg, sometimes less ...... Contain: .......... Hybridoma-SFM (Invitrogen) .......... 0.5% fetal bovine serum (prescreened for ultra low bovine Ig levels) .......... Cell debris (from the hybridoma) ...... Sterile ...... NO sodium azide is added Purified MAbs: ...... MAb concentrations range from 1.0 to 10.0 mg/ml (average ~ 3 mg/ml) ...... Purity = 95% or better ...... Available Aliquot size = usually 1 mg, sometimes more ...... Buffer = PBS ...... Sterile filtered ...... NO sodium azide is added Conjugated MAbs: ...... to Alexa Dyes, Pacific Orange, Pacific Blue, FITC, APC, PE, Biotin and HRP Fragmented MAbs: ...... FAb, F(Ab2)' and Fc All MAbs have scored negative in a mycoplasma screen and several have been MAP tested (a screen for pathogens that infect mice). Many are in inventory for immediate delivery and are for RESEARCH PURPOSES ONLY. 3.) Determining if cell cultures are contaminated with mycoplasma

Genomics Core Facility University of Utah Salt Lake City, UT 84132 (801) 581-2977 Genomics@cores.utah.edu Primary Contact: Derek Warner (801) 581-4936 dwarner@cores.utah.edu Last Updated: 01/04/2012	Services offered: Copy Number Variation (CNV) DNA Analysis Genomics Genotyping Microarray PCR Arrays Real-time qPCR RNA analysis
The University of Utah Genomics Core Facility exists primarily to support university researchers, but is happy to help outside clients with their research needs. The Genomics Core uses the Illumina Bead Xpress, Illumina iScan, ABI Real-time PCR 7900s and ABI 3130xl instrumentation.

DNA Sequencing Core Facility University of Utah Salt Lake City, UT 84132 (801) 581-2976 dna@cores.utah.edu View Website Primary Contact: Derek Warner (801) 581-4736 dwarner@cores.utah.edu Last Updated: 01/04/2012	Services offered: DNA Analysis Sequencing - Capillary Sequencing Sequencing - DNA Sequencing Sequencing - Next-Gen Sequencing
The University of Utah DNA Sequencing Core Facility exists primarily to serve university researchers, but is available to assist in the data generation of outside clients as well. The Core utilizes Capillary electrophoresis as well as Ion Torrent Next-Gen Sequencing.

Genomics & RNA Profiling Core Facility Baylor College of Medicine One Baylor Plaza Room 325E Houston, TX 77030 (713) 798-7699 microarray@bcm.edu View Website Primary Contact: Lisa D. White (713) 798-7607 lisaw@bcm.edu Last Updated: 12/29/2011	Services offered: Computational - Biostatistics Copy Number Variation (CNV) Data Analysis DNA Analysis Genomics Genotyping Microarray Molecular Library Services PCR Arrays Real-time qPCR RNA analysis RNA Integrity Sequencing - DNA Sequencing Sequencing - Next-Gen Sequencing Associations: ABRF Cancer Center MWACD
The Baylor College of Medicine Genomic and RNA Profiling Core or GARP (formerly Microarray Core Facility) provides researchers access to state-of-the-art molecular profiling technology and services, including: * Microarray technology platforms (Affymetrix, Agilent, and NimbleGen) * Illumina Genome Analyzer IIx and HiSeq Next Gen Sequencing * Sample Quality Control * Mouse and Human cDNA Clone Respository * Comprehensive Data Analysis We provide a secure web-based online environment for service requests, sample tracking, data management and billing in the form of MAID, our award-winning microarray information database.

Nanomaterials Characterization Core Facility University of Nebraska Medical Center Pots #42 Omaha, NE 68100 (402) 559-9915 nazar.filonov@unmc.edu View Website Primary Contact: Nazar (402) 559-9915 nazar.filonov@unmc.edu Last Updated: 12/13/2011	Services offered: Data Analysis Light Scattering Mass Spectrometry Analytical Ultracentrifugation Associations: COBRE
The expertise of the members of Center for Drug Delivery and Nanomdicine (CDDN) in studying physico-chemical properties of nanomaterials and polymers has led to establishing the Nanomaterials Characterization Core Facility. The objective of the Core Facility is to provide investigators with state-of-the-art equipment, expertise and custom services for comprehensive study of polymers and nanomaterials. We are welcome all investigators to employ the Core capabilities in your research projects. ---- The Core Facility is equipped with: 1) Inductively Coupled Plasma Mass Spectrometer (ICP-MS) -PerkinElmer Nexion 300Q 2) Analytical Ultracentrifuge (AUC) - BeckmanCoulter Optima XL-I 3)Static Light Scattering - Brookhaven BI-200SM Goniometer

Rhode Island BioBank (RIBB) Brown University Providence, RI 02912 (401) 863-6265 garrett_sullivan@brown.edu View Website Primary Contact: Garrett Sullivan (401) 863-6265 garrett_sullivan@brown.edu Last Updated: 12/09/2011	Services offered: BioBanking Associations: NERTRN
The RIBB will provide a core service by acquiring, processing, tracking and storing biological samples from research study participants. It will provide a secure, state of the art cryogenic storage facility with a sophisticated biospecimen tracking system and will employ personnel trained in the area of biobanking. The RIBB processing lab has research equipment for blood processing and automated sample handling and blood and other biological materials will be processed, labeled with an encrypted vial ID and a bar-code. To ensure viability of the biospecimen for future biological testing, some of the samples will be stored in the vapor phase of liquid nitrogen and tracked using a customized, commercially available biospecimen inventory tracking system. ---- RIBB is a core facility open to Brown investigators. Please contact Garrett Sullivan, Laboratory Manager, for more information. garrett_sullivan@brown.edu

Nevada Proteomics Center University of Nevada, Reno University of Nevada, Reno VPR MS 0330 Reno, NV 89557 (775) 784-4248 rebekahw@unr.edu View Website Primary Contact: Rebekah Woolsey (775) 784-4248 rebekahw@unr.edu Last Updated: 12/07/2011	Services offered: 2d Spectra Acquisition and Interpretation 2D-PAGE Mass Spectrometry Proteomics SDS-PAGE Sequencing - Protein Sequencing Associations: ABRF INBRE
The Nevada Proteomics Center (NPC) provides high quality, high-throughput, cost-efficient proteomic services to all researchers within the Nevada System of Higher Education (NSHE) as well as to academic institutions outside of Nevada. The center is sponsored by the NCRR’s IDeA Networks of Biomedical Research Excellence to enhance the capacity for research in the state of Nevada. The NPC consists of a 2-D gel laboratory and a mass spectrometry facility. In the 2-D gel lab, samples are prepared and separated on 1-D and 2-D electrophoresis gels of all sizes. Differential gel electrophoresis (DIGE) is also routinely performed. The gels are analyzed with PDQuest or DeCyder software and spots of interest are cut with a robotic spot cutter. In the mass spectrometry facility, in-gel and in-solution protease digests are run, and protein discovery and PTM mapping are accomplished using a variety of mass spectrometers. Traditional Edman sequencing of proteins is also provided. NPC instruments include: LTQ Orbitrap XL ETD, LCQ Deca Plus, 4700 MALDI TOF/TOF Analyzer, PolarisQ GC mass spectrometer, ProPrep robotic digestor, Typhoon Trio, ExQuest and ProPic II spot cutters, Procise 492 Protein Sequencer.

UT Southwestern Rodent Behavior Core University of Texas Southwestern Medical Center Department of Psychiatry 5323 Harry Hines Blvd Dallas, TX 75390-9070 (214) 648-5123 shari.birnbaum@utsouthwestern.edu View Website Primary Contact: Shari Birnbaum (214) 648-5123 shari.birnbaum@utsw.edu Last Updated: 11/30/2011	Services offered: Behavioral Phenotyping Associations: MWACD
The UTSW Rodent Behavior Core can assist investigators in all aspects of rodent behavioral testing including the design and execution as well as the data analysis and interpretation. The core facility is equipped to perform a broad battery of behavioral tests to thoroughly phenotype overall motor and sensory function of rats and mice as well as phenotype specific behaviors that are of interest to the investigators (learning and memory, motor function, place/fear conditioning, pain/analgesia, and anxiety-related behaviors). ---- Locomotor activity Anxiety tests Depression tests Conditioned Place Preference Rotarod and Digigait motor analysis Learning and memory tests Addiction/Reward related behaviors Prepulse inhibition pain sensitivity many more

Proteomics and Mass Spectrometry Facility UMass Medical School 222 Maple Ave Fuller Bldg Shrewsbury, MA 01545 (508) 856-8917 proteomics@umassmed.edu View Website Primary Contact: Scott Shaffer (508) 856-8917 scott.shaffer@umassmed.edu Last Updated: 11/18/2011	Services offered: 2D-PAGE Assays and Measurements Mass Spectrometry Proteomics Metabolomics Associations: NERTRN CTSA ABRF
The University of Massachusetts Medical School (UMMS) Proteomics and Mass Spectrometry Facility offers state-of-the-art proteomics and small molecule analyses to UMMS investigators and to the external scientific community at large. Resources include a staff of five individuals (2 Ph.D., 1 M.S., and 2 B.S.) and support for study design, sample preparation, sample fractionation, instrumentation, informatics, data interpretation, and publication assistance. ---- Thermo Scientific LTQ Orbitrap Velos hybrid instrument with EasyLC (Proxeon) nano-HPLC (LC-MS/MS). Thermo Scientific LTQ linear ion trap instrument with Waters nanoAcquity UPLC (LC-MS/MS). Shimadzu Scientific TOF2 hybrid instrument (MALDI TOF-TOF). Shimadzu Scientific QIT hybrid instrument (MALDI QIT-TOF). Waters quadrupole time of flight instrument (Q-TOF) with Waters nanoAquity UPLC(LC-MS/MS). Waters Quattro Premier XE triple quadrupole instrument (QQQ) with either Waters Aquity UPLC or Waters nanoAquity UPLC (LC-MS/MS). Waters Quattro II triple quadrupole instrument (QQQ) with Agilent 5890 gas chromatograph (GC-MS/MS). Supporting 1D and 2D gel electrophoresis platforms and image analysis. Triversa Nanomate (Advion) robot for automated infusion studies. Off-line HPLC separations and fraction collection. *Supporting software platforms include database searching using Mascot (Matrix Science) or Sequest (Thermo Scientific), de novo peptide sequencing using PEAKS (Bioinformatics Solutions), and comparative proteomics using Scaffold (Proteomics Software) or ProteoIQ (NuSep).

MidSouth Bioinformatics Center at UALR University of Arkansas at Little Rock EIT 303 2801 S. University Ave. Little Rock, AR 72204-1099 (501) 569-8501 bioinformatics@ualr.edu View Website Primary Contact: Roger Hall, Technical Director (501) 569-8074 rahall2@ualr.edu Last Updated: 11/16/2011	Services offered: Computational - Application Development Computational - Bioinformatics Computational - Biostatistics Computational - High Performance Computing Data Analysis Genomics Outreach Proteomics Associations: INBRE
The MidSouth Bioinformatics Center (MBC) at UALR provides computational and consulting resources for researchers and students in Arkansas. Founded in 2002 by Dr. Steve Jennings (Executive Director), the Center has been instrumental in supporting the growth of bioinformatics within the State of Arkansas and the MidSouth region of the US. The MBC's primary clients are the graduate students in the UALR/UAMS bioinformatics graduate program, the undergraduate institutions and researchers affiliated with the Arkansas NIH NCRR INBRE program, and researchers at the medical institutions in Arkansas (the University of Arkansas for Medical Sciences, the VA Hospitals, and Arkansas Children's Hospital). Additionaly, Center staff provide outreach and workshops on bioinformatics-related technologies to a variety of audiences and provides technical support to the MidSouth Computational Biology and Bioinformatics Society (www.MCBIOS.org).

Epigenome Center University of Southern California 1450 Biggy St NRTG514 Los Angeles, CA 90033 (323) 442-7988 cnicolet@usc.edu View Website Primary Contact: Charles Nicolet (323) 442-7988 cnicolet@usc.edu Last Updated: 11/15/2011	Services offered: Computational - Bioinformatics Data Analysis Genomics Genotyping Library Services Microarray Molecular Library Services RNA Integrity Sequencing - Next-Gen Sequencing Associations: ABRF Cancer Center WCASRD
Hi-Seq 2000 and GAIIX next gen sequencing, as well as library preparations for genomic DNA, ChIP-seq, RNA-seq, smRNA-seq, and bisulfite sequencing. Bar coding available on all library types. Substantial qc metrics and bioinformatics provided as part of standard service. Illumina gene expression chips are also run in the core. ---- HiSeq 2000 and 2 cBots GAIIX (2) Agilent BIoanalyzer, Bio-Rad Experion, nanodrop, ABI HT7900

Proteomic and Metabolomic Core Facility Redox Biology Center E 156 Beadle Center Lincoln, NE 68588 (402) 472-5097 nmadayiputhiya2@unlnotes.unl.edu View Website Primary Contact: Nandakumar Madayiputhiya (402) 472-5097 nmadayiputhiya2@unlnotes.unl.edu Last Updated: 11/15/2011	Services offered: Assays and Measurements Computational - Application Development Mass Spectrometry Proteomics Protocol Development/Clinical Trial Coordination Metabolomics
The Laboratory for Proteomics and Metabolomics analysis at the Redox Biology Center (RBC) supports scientists within the RBC and the University of Nebraska. The Core provides all the tools of modern functional proteomics and metabolomics. Equipped with cutting edge mass spectrometry based state-of-the-art technologies for proteomics and metabolomics; protein and metabolite profiling, protein identification, protein and peptide fractionation and quantitation, differential expression analysis, shot gun proteomics, targeted MRM metabolite and drug analysis from clinical samples, MRM quantitative proteomics, personalized experimental design consultation and comprehensive individualized bioinformatics support.

Proteomics Core Facility University of Maryland 2225 Bioscience Research Building College Park, MD 20742 (301) 405-8425 yanwang@umd.edu View Website Primary Contact: Yan Wang (301) 405-6764 Last Updated: 11/14/2011	Services offered: Mass Spectrometry Proteomics Associations: ABRF
The Proteomics Core Facility is a fee for service facility supported by the College of Chemical and Life Sciences. The facility offers service to all researchers in the region, with priority given to users from the College of Chemical and Life Sciences. Routine service includes molecular weight determination of intact proteins by LCMS and protein identification through tryptic digestion, LCMSMS analysis and database searching. Protein samples can be submitted as lyophilized powders, solutions, or gel slices. The facility also welcomes collaborative research on projects beyond routine services. ---- Shimadzu 2D Nano HPLC Thermo LTQ Orbitrap XL with ETD Agilent 3100 OFFGEL fractionator

Mouse Phenotyping University of Hawaii BSB 311, John A. Burns School of Medicine 651 Ilalo St. Honolulu, HI 96813 (808) 692-1567 WAB@hawaii.edu View Website Primary Contact: William Boisvert (808) 692-1567 William Boisvert Last Updated: 11/10/2011	Services offered: Animal Husbandry DNA Analysis Surgical Services Ultrasonic Imaging Associations: NICL COBRE
Services provided include animal husbandry and genotyping, murine echocardiography,reporter gene imaging, hemodynamic monitoring, tail vein plethysmography, surgical procedures including infarction, aortic banding, tail vein injection, pump implantation, and training in all of these procedures. ---- We have an active program in ultrasound-targeted microbubble destruction to deliver expression constructs to various organs in the mouse. Although it is probably premature to offer this as a service, and our distance from the mainland makes this impractical, we are happy to provide training in this potentially useful technology.

Vermont Advanced Computing Core (VACC) University of Vermont 217 Farrell Hall 210 Colchester Avenue Burlington, VT 05405 (802) 656-8867 vacc@uvm.edu View Website Primary Contact: Melody Brown Burkins (802) 656-8867 mburkins@uvm.edu Last Updated: 11/10/2011	Services offered: Computational - Application Development Outreach High Performance Computing (HPC) Associations: NERTRN EPSCoR CTSA
The Vermont Advanced Computing Core (VACC), in partnership with UVM Enterprise Technology Services (ETS), supports computationally-intensive research and high performance computing (HPC) services at the University of Vermont (UVM) and its external partners. VACC is also a hub for advanced computing outreach and HPC education, supporting two "Campus Champions" to connect researchers to the national NSF TeraGrid services, holding HPC consulting clinics and campus-wide workshops, and coordinating cyberinfrastructure planning with programs throughout Vermont and the northeast. ---- Please see http://www.uvm.edu/~vacc

Genomics Core Facility UMass Medical School 55 Lake Ave North S5-116 Worcester, MA 01655 (508) 856-4076 Phyllis.Spatrick@umassmed.edu View Website Primary Contact: Phylis Spatrick (508) 856-4076 Phyllis.Spatrick@umassmed.edu Last Updated: 11/08/2011	Services offered: Genomics Microarray RNA Integrity Associations: NERTRN ABRF
The Genomics Core Facility at UMass Medical School is an excellent resource for the UMass and Worcester area research community featuring high-density microarray technology for both Affymetrix GeneChip and glass slide array processing. • Sample preparation and Affymetrix instrumentation for 3' IVT Expression, Gene ST, Exon, and Tiling Arrays, as well as CustomSeq array hybridization and scanning. • Glass slide array scanning using the Agilent G6525CA Scanner enabled for 2 micron scanning. Expression, ChIP-chip, miRNA and DNA microarray. • Glass slide hybridization capabilities with the Nimblegen Hybridization System • Genespring GX Analysis Software available Genomics Core Facility University of Massachusetts Medical School Department of Molecular Genetics and Microbiology S5-116, S5-125, S5-129 55 Lake Avenue, North ---- Affymetrix Fluidics and Scanning Agilent Scanning Agilent Genespring Workstation Agilent Bioanalyzer

Cytometry Core Facility New York University Office of Collaborative Science 540 First Ave SK3 Lab8 New York, NY 10016 (212) 263-5907 cytometry@med.nyu.edu View Website Primary Contact: Peter Lopez (212) 263-0635 Peter.Lopez@med.nyu.edu Last Updated: 11/04/2011	Services offered: Cell Sorting Flow Cytometric Analysis Associations: Cancer Center ABRF ISAC
MoFlo cell sorter Reflection parallel sorter Full containment BSL-3 cell sorting (iCyt Synergy) 18-color LSRII High speed cell sorting Non-NYU investigators welcome BSL3 sorting

Genome Technology Access Center Washington University School of Medicine Dept of Genetics Campus Box 8232 St. Louis, MO 63110 (636) 286-1256 scrosby@wustl.edu View Website Primary Contact: Seth Crosby (314) 286-1256 gtac@genetics.wustl.edu Last Updated: 11/03/2011	Services offered: Copy Number Variation (CNV) Data Analysis DNA Analysis Genomics Genotyping Microarray Real-time qPCR RNA analysis RNA Integrity Sequencing - DNA Sequencing Sequencing - Next-Gen Sequencing QuantiGene Plex Associations: MWACD ABRF
We are a full service facility, from hypothesis to publication. Microarry: expression and genotyping illumina Affymetrix Agilent Nimblegen Sequecing: RNA, gDNA, ChIP, Capture *HiSeq 2000 ---- Liquid-handling automation for microarray and library prep Sequencing •Illumina HiSeq2000 (2) Microarray •Agilent G2565CA Microarray Scanning System •Illumina iScan •Illumina Beadstation 500GX (2) •Affymetrix Genechip 3000 •Molecular Devices Genepix 4000 B RNA/DNA QC •Nanodrop ND-1000 spectrophotometer •Eppendorf BioPhotometer •Agilent 2100 Bioanalyzer

Imaging Core Seattle Biomedical Research Institute 307 Westlake Ave North, Suite 500 Seattle, WA 98109-5219 (206) 256-7498 tracy.mitchell@seattlebiomed.org View Website Primary Contact: Tracy Mitchell Last Updated: 10/31/2011	Services offered: Cell Imaging Confocal Microscopy Microscopy Molecular Imaging Phosphor Imaging Associations: WCASRD
The Imaging Core provides advanced fluorescence/auto-radiography instrumentation and related consulting services. The imaging equipment and software allows researchers to describe cell changes and function under varying conditions with specialized microscopes and to characterize molecules following gel separation and image detection. ---- DeltaVision RT optical/digital-sectioning microscope Leica SP2 laser scanning confocal microscope Nikon Eclipse E600 upright microscope Nikon TE2000U inverted microscope Odyssey Imaging System Storm 860 Phosphorimager

Seattle BioMed Sequencing Core Facility Seattle Biomedical Research Institute 307 Westlake Avenue North, Suite 500 Seattle, WA 98109-5219 (206) 256-7341 sequencing@seattlebiomed.org View Website Primary Contact: Ellen Sisk (206) 256-7400 ellen.sisk@seattlebiomed.org Last Updated: 10/31/2011	Services offered: Genotyping Nucleic Acid Extraction Real-time qPCR Sequencing - Capillary Sequencing Sequencing - DNA Sequencing Associations: WCASRD
The facility is CLIA certified and offers sequencing and fragment analysis services to academic and non-academic users in Seattle's scientific community, as well as international scientists and organizations. Our service provides cost-effective solutions for small laboratories without access to sequencing technology, in addition to large organizations with demands that exceed "in house" capacity. ---- 3730XL DNA Analyzer 7500 Fast Real-Time PCR system

Imaging, Cytometry and Microfluidics (ICM) Core Institute for Systems Biology 401 Terry Ave North Seattle, WA 98109 (206) 732-1432 noel.blake@systemsbiology.org Last Updated: 10/28/2011	Services offered: Cell Imaging Cell Sorting Flow Cytometric Analysis Microscopy Associations: ABRF ISAC WCASRD
Will fill in later

Center for Genome Research and Biocomputing Oregon State University 3021 ALS CGRB-OSU Corvallis, OR 97331 (541) 737-3413 Caprice.Rosato@oregonstate.edu View Website Primary Contact: Caprice Rosato Last Updated: 10/28/2011	Services offered: Computational - Application Development Computational - Bioinformatics Computational - High Performance Computing Confocal Microscopy Genomics Laser Capture Microdissection Microarray Microscopy Phosphor Imaging Real-time qPCR RNA analysis RNA Integrity Sequencing - Capillary Sequencing Sequencing - DNA Sequencing Sequencing - Next-Gen Sequencing Support Services - Central Laboratory Supply Associations: ABRF WCASRD
Services for fee: DNA Sequencing, Genotyping, Gene expression microarrays, Bioanalyzer. Multi-user instruments: Confocal and Laser Capture Microdissection Microscopes, Nanodrop, qRT-PCR, phosphorimager, fluorescent plate reader. ---- Illumina HiSeq 2000, Roche 454 GS Jr., AB 3730, AB 7500 FAST, Zeiss LSM 510 Meta, GE Storm

DNA Technologies and Expression Analysis Cores University of California--Davis One Shields Ave Davis, CA 95616 (530) 754-5281 rwkim@ucdavis.edu View Website Primary Contact: Ryan Kim (530) 754-4988 rwkim@ucdavis.edu Last Updated: 10/27/2011	Services offered: Genomics Genotyping Library Services Sequencing - DNA Sequencing Sequencing - Next-Gen Sequencing Support Services - Shared Instrumentation Oversight & Maintenance Associations: ABRF WCASRD
Genotyping on all the Illumina platforms, sequencing on Illumina GAIIx, HiSeq2000, MiSeq,and PacBio RS, array services for NimbleGen and Illumina, chromatin immunoprecipitation and methylated DNA immunoprecipitation services.

RWJMS DNA Core Facility UMDNJ-RWJMS Research Annex 663 Hoes Lane Piscataway, NJ 08854 (732) 235-4934 dnalbweb@umdnj.edu View Website Primary Contact: Lee Ann Schein (732) 235-3446 scheinla@umdnj.edu Last Updated: 10/26/2011	Services offered: DNA Analysis Genomics Genotyping oligo synthesis PCR Arrays Real-time qPCR Sequencing - Capillary Sequencing Sequencing - DNA Sequencing Viral Vectors Associations: ABRF
The UMDNJ-RWJMS DNA Core Facility is committed to provide quality, inexpensive services to scientists at UMDNJ, Rutgers University, and other scientific institutions and companies. Services are provided for automated DNA sequencing, DNA/RNA oligonucleotide synthesis, DNA fragment analysis, SNP analysis,and quantitative Real-Time PCR. The Core also offers a Human shRNA library and an Applied Biosystems Freezer Program .

Hubbard Center for Genome Studies University of New Hampshire 35 Colovos Road Gregg Hall room 447 Durham, NH 03824 (603) 862-2474 jobriaha@unh.edu View Website Primary Contact: Dr Kelley Thomas (603) 862-2470 kelley.thomas@unh.edu Last Updated: 10/25/2011	Services offered: Computational - Bioinformatics DNA Analysis Genomics Genotyping RNA analysis RNA Integrity Sequencing - Capillary Sequencing Sequencing - DNA Sequencing Associations: INBRE
We provide DNA sequencing using ABI 3130. We also have a Agilent 2100 Bioanalyzer to analyze DNA and RNA samples as well as a NanoDrop Spec.

UCLA W. M. Keck Proteomics Center University of California-Los Angeles Dept. Chemistry and Biochemistry Box 156905 / MSB 1424 Los Angeles, CA 90095-1569 (310) 206-0432 masondej@ucla.edu View Website Primary Contact: Melissa Sondej (310) 206-0432 masondej@ucla.edu Last Updated: 10/24/2011	Services offered: 2D-PAGE Data Analysis Mass Spectrometry Proteomics SDS-PAGE Support Services - Shared Instrumentation Oversight & Maintenance Western Blot Bioinformatics Associations: ABRF
The UCLA W. M. Keck Proteomics Center is a part of the UCLA Molecular Instrumentation Center and was established in 2002 to provide a first-rate proteomic core facility for investigators wishing to apply proteomic analysis in their research. The UCLA Proteomics Center has three Ph.D. level staff members who are experts in sample preparation, 2-D gel and other electrophoresis techniques, bioinformatics, and mass spectrometry. This facility contains all the equipment necessary for running 1-D and 2-D gels, transferring mini and mid-size gels, gel imaging, 1-D and 2-D gel analysis, spot cutting, in-gel digestion, 1-D and 2-D gel analysis, protein/peptide identification and data mining for a large variety of proteomics studies. In addition, we provide expert guidance in sample preparation for 1-D and 2-D gel electrophoresis and mass spectrometry, experiment design, gel staining including multiplex staining that compares total protein staining to phosphoprotein and glycoprotein staining, analysis of protein expression patterns, western and lectin blots, in-gel trypic digestion and operating the various instruments. We also offer Standard Mass Spectrometric Analysis including Peptide-Mass Fingerprinting and Protein Characterization by MALDI-TOF, LC-MS/MS services including Protein Identification from In-Gel or In-Solution Digestion and Intact Protein Characterization, and Quantitative Mass Spectrometry including Discovery Mode Quantitative Proteomics Using Stable Isotope Labeling Reagents or SILAC and Targeted Quantitation or Validation of Small Molecules or Peptides Using Selected Reaction Monitoring (SRM). Our center operates as an open access center where qualified users are encouraged to perform their own sample analysis under the training and guidance of our personnel when appropriate. ---- Bio-Rad electrophoresis cells for running 1- and 2-D gels; Bio-Rad Fx Fluorescence Imager and GS-800 Densitometer for imaging; DIGILAB Genomic Solutions ProPicII spotcutter; Thermo LTQ FT MS with Eksigent NanoLC-2D HPLC; Thermo LTQ Orbitrap XL MS with Eksigent NanoLC-2D HPLC and a Bruker SolariX-hybrid Qq-FTMS equipped with a 15 Tesla Magnet System, quantitative software for 2-D gel and mass spectrometry data analysis

Plant-Microbe Genomics Facility Ohio State University BioSci Bldg - Room 420 484 West 12th Avenue Columbus, OH 43210-1214 (614) 247-6204 pmgf@osu.edu View Website Primary Contact: Michael Zianni (614) 247-6204 pmgf@osu.edu Last Updated: 10/22/2011	Services offered: 2D-PAGE DNA Analysis Genomics Genotyping Nucleic Acid Extraction Outreach Proteomics Real-time qPCR RNA analysis RNA Integrity SDS-PAGE Sequencing - Capillary Sequencing Sequencing - DNA Sequencing Sequencing - Next-Gen Sequencing Sequencing - Pyrosequencing Surface Plasma Resonance (SPR) Western Blot Associations: MWACD ABRF
The facility provides services primarily to Ohio State University, but all researchers are welcome. The facility studies DNA to proteins from all organisms through 7 main services: 1) DNA sequencing, 2) Genotyping, 3) Promoter Characterization, 4) Real-time quantitative PCR, 5) Proteomics, and 6) Biomolecular Interaction Analysis and 7) Massively Parallel Pyrosequencing. ---- Protean IEF Cell, BioRad Criterion SDS-PAGE Cell, BioRad Criterion Protein Transfer Cell, BioRad Proteome Works Spot Cutter, BioRad VersaDoc Imaging System, BioRad Apollo100 robotic microcycler, IntegenX iCycler thermal cycler, BioRad GeneAmp PCR System 9700, Applied Biosystems Biomek FX liquid handling robot system, Beckman CFX96 Real-Time System, BioRad iQ real-time PCR System, BioRad 428 Array Scanner, Affymetrix Biacore T100, GE Health Sciences 3730 DNA Analyzer, Applied Biosystems Spectrafluor Plus Microplate reader, Tecan 454 FLX Titanium Genome Sequencer, Roche Bioanalyzer electrophoresis system, Agilent Z1 Coulter Counter, Beckman Auto T4 Cell Counter, Nexcelom TissueLyser II, Qiagen

NMR Facility University of Vermont 82 University Place Cook Physical Sciences, Rm A-215 Burlington, VT 05405 (802) 656-3125 P.Deker@uvm.edu Primary Contact: P. Bruce Deker, Ph.D. (802) 656-3125 P.Deker@uvm.edu Last Updated: 10/21/2011	Services offered: 2d Spectra Acquisition and Interpretation Nuclear Magnetic Resonance (NMR) Spectroscopy Associations: NERTRN
The facility has both Bruker(ARX console with xwinnmr software) and Varian(Unity Inova console with vnmr6.1 software) 11.7 telsa (500 MHz) liquid state NMR platforms. The Bruker has an additional low resolution solid state capability while the Varian has an additional z-axis PFG capability. Both 5 and 10 mm direct as well as inverse detection probes with temperature ranges from -80oC to +80oC are available. The following is a partial list of nuclei which have been observed: 1H, 2H, 7Li, 11B, 13C, 14N, 15N, 17O, 19F, 27Al, 29Si, 47Ti, 51V, 79Br, 81Br, 87Rb, 91Zr, 97Mo, 109Ag, 119Sn, 129Ze, 195Pt. Academic new user training and walk up user accounts are available at an annually expense adjusted rate (currently $13/hour). Organic structure, configuration, and conformation elucidation service is available at no additional charge by special arrangement with the facility manager. This collaborative service typically utilizes COSY, TOCSY, ROESY, HMQC, HMBC, and 2DJ experiments to prove most 1J, 2J and 3J homonuclear and heteronuclear couplings, as well as to approximate distance and dihedral restrictions.

RI-INBRE Centralized Research Core Facility University of Rhode Island Fogarty Hall 222 41 Lower College Road Kingston, RI 02881 (401) 874-5731 inbrelab@etal.uri.edu View Website Primary Contact: Aftab Ahmed, Ph.D. (401) 874-5731 aahmed@uri.edu Last Updated: 10/21/2011	Services offered: 2D-PAGE Assays and Measurements Biochemical Analysis Cell Culture Cell Imaging Cell Sorting Computational - Bioinformatics Data Analysis Flow Cytometric Analysis Gel Chromatography Genomics Mass Spectrometry Microarray Microscopy Molecular Imaging Nucleic Acid Extraction Optical Imaging Outreach PCR Arrays Phosphor Imaging Protein Extraction/Purification Proteomics Real-time qPCR RNA analysis SDS-PAGE Sequencing - Protein Sequencing Spectroscopy Support Services - Glass Washing/Autoclaving Tissue Culture Western Blot MALDI-TOF MS Associations: INBRE NICL
Research Core Facility The facility is supported by the Rhode Island IDeA Network of Biomedical Research Excellence (RI-INBRE) grant and by the institutions participating in the grant. The facility provides research and training support to RI-INBRE participants and to the Rhode Island biomedical research community. It is equipped with instrumentation for biomedical, pharmaceutical, and biotechnological research. ---- Poster printing FPLC/HPLC Purification Flash Chromatography Whole Animal Imager ---- Whole Animal Imager

Engineering and Mathematical Sciences Research Facilities - Materials Science University of Vermont A405 Cook Burlington, VT 05405 (802) 656-3390 rheadrick@uvm.edu View Website Primary Contact: Randy Headrick (802) 656-3390 rheadrick@uvm.edu Last Updated: 10/21/2011	Services offered: Light Scattering Microscopy Small-Angle Neutron Scattering Spectroscopy Thermal Analysis Ultrasonic Imaging X-Ray Crystallography X-ray Diffraction and Scattering Associations: NERTRN
Teaching and research facilities of the Materials Science program are primarily located in Cook Physical Sciences Building (Physics, Chemistry) and Votey Hall (Electrical and Mechanical Engineering). Votey and Cook are adjacent to one another and are located on the central campus of UVM. Many of the faculty in the Materials Science Program have developed individual laboratories to conduct their chosen research topics. In those laboratories the capabilities to perform a variety of experimental investigations including thin film growth, polymer studies, nondestructive studies, micromechanical measurements, and chemical synthesis have been developed. The Materials Science Program shares the Chemistry Department Facilities and Microscopy Imaging Center. Individual departments also maintain user laboratories which include the sophisticated characterization facilities listed below. Light Scattering Small-Angle Neutron Scattering X-ray Diffraction and Scattering Material Processing Micromechanical Force Measurements Microscopy Spectroscopy Noninvasive Ultrasonic Imaging Rheology Thermal Analysis

UW Biotech Center-DNA Sequencing Facility University of Wisconsin-Madison 425 Henry Mall, Room 1250 Madison, WI 53706 (608) 262-4657 nextgen-seq@biotech.wisc.edu View Website Last Updated: 10/20/2011	Services offered: Genomics Genotyping Nucleic Acid Extraction oligo synthesis Sequencing - Capillary Sequencing Sequencing - DNA Sequencing Sequencing - Next-Gen Sequencing Associations: MWACD ABRF
The DNA Sequencing Facility provides DNA sequencing, DNA genotyping, and DNA extraction services to clients at public and private institutions. Both Sanger sequencing and next generation sequencing platforms are available, providing inexpensive sequencing of isolated fragments or massively parallel sequencing of random fragments. DNA genotyping services are offered for researchers interested in SNP, T-RFLP, or microsatellite (VNTR, SSR) genotyping. ---- Illumina HiSeq 2000 (2) Illumina GA IIx (1) Roche GS FLX (with extended read module) Synergy 2 plate reader Bio-Rad Bio-Plex 200 (Luminex) Roche LightCycler 480 Real-Time PCR System Beckman Coulter Biomek FX w/ 96 channel head Innovadyne Nanodrop II Eppendorf epMotion 5075

Nanoimaging Core Facility University of Nebraska Medical Center University of Nebraska Medical Center, 986025 Nebraska Medical Center Omaha, NE 68198-6025 (402) 559-2753 lshlyakhtenko@unmc.edu, ylyubchenko@unmc.edu View Website Primary Contact: luda shlyakhtenko (402) 559-1974 lshlyakhtenko@unmc.edu Last Updated: 10/20/2011	Services offered: Atomic Force Spectroscopy Data Analysis
The facility provides service and expert advice to researches in their projects related to nanoimaging studies of molecular systems at single molecule and single cell levels utilizing Atomic Force Microscope and combination of Atomic Force Microscope with Fluorescence Optical Microscope ---- AFM microscope from Asylum Research mounted on the inverted fluorescence microscope from Olympus. The stand-alone MultiMode AFM from Veeco-Bruker Instruments

Proteomics Core of the COBRE Center for Cancer Res.Dev. Rhode Island Hospital RI Hospital COBRE CCRD Proteomics Coro Ctr., One Hoppin St., Ste. 4.206 PROVIDENCE, RI 02903 (401) 444-2871 Esmith6@lifespan.org View Website Primary Contact: Elizabeth Smith (401) 444-2871 Esmith6@lifespan.org Last Updated: 10/20/2011	Services offered: Computational - Bioinformatics Data Analysis Mass Spectrometry Protein Extraction/Purification Proteomics SDS-PAGE Sequencing - Capillary Sequencing Sequencing - Protein Sequencing Spectroscopy Western Blot Associations: COBRE EPSCoR INBRE
The Proteomics Core facilitates cancer research by: Assisting investigators in choosing appropriate methods and techniques for specific research objectives Providing a means for investigators to become directly involved in protein analysis at a level not possible with commercial suppliers Providing expertise in protein bioinformatics

Biocircuit analysis Maine Medical Center Research Institute 81 Research Drive Scarborough, ME 04074 (207) 396-8125 karolm@mmc.org Primary Contact: Leif Oxburgh, DVM, PhD (207) 396-8115 oxburl@mmc.org Last Updated: 10/19/2011	Services offered: Cell Imaging Microarray Microscopy Real-time qPCR Associations: NERTRN COBRE
The goal of the facility is to provide investigators with access to training and instrumentation to conduct transcriptome analyses and automated microscopy. Microarray analyses are performed through a collaboration with the COBRE-supported University of Vermont Medical School Microarray facility.

Trudeau Institute Molecular Biology Core Facility Trudeau Institute 154 Algonquin Avenue Saranac Lake, NY 12983 (518) 891-3080 x 115, 105 OR 181 MBCF@Trudeauinstitute.org View Website Primary Contact: Scottie Adams (518) 891-3080 x 115 sadams@trudeauinstitute.org Last Updated: 10/19/2011	Services offered: Assays and Measurements Data Analysis DNA Analysis Gel Chromatography Genomics Genotyping Nucleic Acid Extraction PCR Arrays Protein Extraction/Purification Real-time qPCR RNA analysis RNA Integrity SDS-PAGE Tissue Culture Western Blot Associations: ABRF
The Trudeau Institute Molecular Biology Core Facility is a multi service core. Services provided include production, purification and labeling of Major Histocompatibility (MHC) Class I and Class II tetramers for Fluorescent Activated Cell Sorter (FACS) analysis, real-time PCR measurement of gene expression and viral loads, mtDNA:nDNA RT-PCR assays, knock-out mouse screening, recombinant protein expression/purification for antibody detection, monoclonal and polyclonal antibody production and in vivo immunization, RNA and protein analysis using microfluidics technology, and ELISAs. A recent addition to the MBCF repertoire of skills is expertise in cloning, retroviral construction for gain of function, loss of function (shRNA), virus production and transfection. A variety of simple services such as primer design, primer ordering, stock primers, peptide ordering and Taq production save the investigators time, effort and money. Education and training are provided for all techniques and for using shared MBCF instrumentation, such as spectraphotometers, real-time PCR equipment, tissue prep and image capture/analysis equipment. ---- AB 7500 FAST Real Time PCR System AB Prism 7700 Real Time PCR System BioRad Experion BioRad Chemi Doc BioRad Molecular Imager BioRad Biologic Protein Purification System (2) Fisher Sonic Dismembrator NanoDrop 1000 Spectrophotometer Qiagen TissueLyser II

UC Davis Genome Center Proteomics Core Facility University of California--Davis Proteomics Core, Room 1414 GBSF 451 East Health Sciences Drive Davis, CA 95616-8816 (530) 754-9474 bsphinney@ucdavis.edu View Website Primary Contact: Brett Phinney (530) 754-5298 bsphinney@ucdavis.edu Last Updated: 10/19/2011	Services offered: Data Analysis Mass Spectrometry Proteomics Amino Acid Analysis, Edman Sequencing Associations: ABRF
The UC Davis Proteomics Facility offers protein analysis via amino acid analysis, Edman sequencing and tandem mass spectrometry techniques including protein identification, post-translational modification discovery, label free quantitation and protein cross-linking analysis. The facility utilizes state of the art instrumentation, including a Thermo LTQ-FT Ultra. Our group utilizes open source software and uses techniques and protocols that are made publicly available. Using open source software allows users to analyze their own data without significant monetary investment. We also offer data analysis and sample preparation classes to UC Davis and the community at large, including a week long hands on summer short course. Our group operates as an open core facility with the Genome Center on the UC Davis campus ---- Please see http://proteomics.ucdavis.edu/equipment/

Dartmouth Molecular Biology Core Facilty Dartmouth Medical School HB 7650, Remsen 243A Dartmouth Medical School Hanover, NH 03755 (603) 650-6546 mbcore@dartmouth.edu View Website Primary Contact: Christian Lytle (603) 650-6546 Christian.H.Lytle@Dartmouth.edu Last Updated: 10/19/2011	Services offered: DNA Analysis Genotyping Mass Spectrometry Real-time qPCR Sequencing - Capillary Sequencing Sequencing - DNA Sequencing Support Services - Shared Instrumentation Oversight & Maintenance Associations: ABRF Cancer Center COBRE INBRE
The Dartmouth Molecular Biology Core Facility offers services and support for DNA sequencing, DNA fragment analysis and a shared instrument service for many applications. ---- ABI 3730 DNA Analyzer Perkin Elmer Janus Automated Workstation ABI 2700 GeneAmp PCR System (2) ABI Model 7500 Fast RealTime PCR System (2) Agilent 2100 Bioanalyser Thermo NanoDrop ND1000 Spectrometer BioRad ChemiDoc XRS+ Molecular Dynamics 9410 and 8600 Typhoon Imagers Molecular Dynamics 860 Storm Imager Kodak Model 2000 X-Ray Film Processor ABI Voyager DE Pro MALDI-Mass Spectrometer

Proteomics and Metabolomics Core Pennington Biomedical Research Center 6400 Perkins Road Baton Rouge, LA 70808 (225) 763-2534 indu.kheterpal@pbrc.edu View Website Primary Contact: Indu Kheterpal (225) 763-2534 indu.kheterpal@pbrc.edu Last Updated: 10/19/2011	Services offered: 2D-PAGE Mass Spectrometry Protein Extraction/Purification Proteomics Metabolomics
The mission of the Proteomics and Metabolomics Core Facility at PBRC is to provide scientific and technical expertise and resources to enable researchers to apply proteomics and metabolomics for biomedical and biological studies. The proteomics capabilities of the facility include protein identification, protein quantification, differential proteomics, protein modification analysis, characterization of protein complexes and protein structure determination. The metabolomics capabilities of the facility include global metabolomics, metabolite identification, metabolite quantification and targeted metabolite profiling. The facility has resources and expertise to perform both proteomics and metabolomics experiments for a variety of samples such as cells, sub-cellular fractions, secreted media, tissues and biological fluids. The facility is equipped to carry out a range of experiments and studies as listed under Services. In addition to our current capabilities, we are always open to new techniques and measurements, and we will work with you to develop and apply new methodologies for your research. ---- Metabolite profiling Lipid analysis Small molecule quantification Peptide quantification ---- SYNAPT HDMS (Waters Corp.): This instrument is capable of operating in both quadrupole time-of-flight (Q-TOF) and ion mobility mode and is primarily used for unbiased detection of peptides and metabolites for proteomics and metabolomics applications, respectively. A nanoAcquity UPLC system with 2D technology is directly interfaced to nanospray ionization source of SYNAPT HDMS for proteomics applications. An Acquity UPLC system is interfaced to ESI/atmospheric pressure chemical ionization (APCI) source for metabolomics applications. An atmospheric pressure photoionization (APPI) source is also available for Synapt HDMS. XEVO TQ (Waters Corp.): It is a tandem quadrupole mass spectrometer with a dedicated acquity UPLC system for highly selective and sensitive detection of metabolites and peptides. 4000 QTRAP (AB Sciex): It is a hybrid linear ion trap and triple quadrupole mass spectrometer interfaced with a nano-LC system from Eksigent Technoloiges for biomolecular analysis. A dedicated UFLC system from Shimadzu Corp. is also available for interfacing to ESI source of 4000 QTRAP. Q-TOF micro (Waters Corp.): It is a quadrupole time-of-flight instrument with a dedicated capillary LC system as the front interface. MALDI-TOF MS (Waters Corp.): It is a MALDI - time-of-flight instrument for MS analysis of biomolecules.

Transgenic Mouse Research Facility Stanford University CCSR Building, Room 0137 Stanford University Stanford, CA 94305 (650) 498-7604 ychen@stanford.edu View Website Primary Contact: Yanru Chen-Tsai (650) 498-7604 ychen@stanford.edu Last Updated: 10/18/2011	Services offered: Assisted Reproductive Technologies (Rodent IVF, ICSI) Cell Culture Clinical Imaging - Small Animal (X-ray, Ultrasound, microCAT, MRI) Embryo Cryopreservation & Recovery Services Histology Molecular Construct Services Tissue Culture Transgenics Xenograft / Xenotransplantation human ES/iPS cell, teratoma
The Stanford Transgenic Research Facility is set to produce your transgenic and knockout mouse models. The facility provides low-cost DNA microinjection services, ES cell manipulation, and microinjection services. The facility also provides services on DNA constructs, timed pregnant females, strain rederivation, embryo and sperm cryopreservation and IVF procedures for resurrecting frozen sperms. The Preclinical oncology lab of the facility provides animal tumor model services including xenograft, dosing, imaging, toxicity and PK studies. The ES cell group of the facility provides human ES/iPS cell culture, teratoma formation analysis in addition to mouse ES cell services.

WestCore (Western S.D. DNA Core Facility Black Hills State University 1200 University Blvd, Unit 9053 Spearfish, SD 57799-9053 (605) 642-6854 cynthia.anderson@bhsu.edu View Website Primary Contact: Dr. Cynthia Anderson (605) 642-6854 cynthia.anderson@bhsu.edu Last Updated: 10/18/2011	Services offered: Genotyping Library Services Sequencing - DNA Sequencing marker development, microsatellite marker development Associations: INBRE
The Western South Dakota DNA Core Facility (WestCore) was established in 2003 as part of the South Dakota Biomedical Research Infrastructure Network (SD-BRIN). We complement the Proteomics and Genomics BRIN core facilities. Our primary services include DNA sequencing, genotyping, marker development and library construction. Priority is given to SD-BRIN/INBRE research, but services are also provided to non-BRIN/INBRE university researchers as well as government agencies and some private organizations. Westcore is a fully staffed laboratory with automated DNA instrumentation, and has a federally approved quality management plan.

Biomolecular Core Laboratory Nemours Center for Pediatric Research Alfred I. duPont Hospital for Children 1600 Rockland Rd Wilmington, DE 19803 (302) 651-6712 mbcore@nemours.org View Website Primary Contact: Jennifer Holbrook (302) 651-6712 mbcore@nemours.org Last Updated: 10/18/2011	Services offered: Assays and Measurements Copy Number Variation (CNV) Data Analysis DNA Analysis Genomics Genotyping Microarray Nucleic Acid Extraction oligo synthesis Outreach PCR Arrays Protocol Development/Clinical Trial Coordination Real-time qPCR RNA analysis RNA Integrity Sequencing - Capillary Sequencing Sequencing - DNA Sequencing Sequencing - Pyrosequencing Support Services - Shared Instrumentation Oversight & Maintenance Forensics Associations: NICL COBRE INBRE Cancer Center ABRF
Statement of purpose: A resource for the staff of the Alfred I. duPont Hospital for Children, Nemours affiliates, COBRE / INBRE investigators and outside customers, the Biomolecular Core Laboratory is a centralized, state-of-the-art facility that offers expertise in molecular genetics and genomics. The service lab operates according to the policies set forth by the federal CLIA standards to provide high data integrity and to maintain appropriate safeguards for patient confidentiality. Services provided include: Expression Analysis: • Affymetrix Microarray (global gene expression 3’IVT & WT assays) • Pathway-focused Real-Time qPCR (mRNA and miRNA) Genotyping: • Allelic Discrimination Probes (SNP Real-Time PCR) • Affymetrix Microarray (CNV CytoScan, SNP arrays) • Fragment Analysis (Capillary Electrophoresis up to 1200 bp) • DNA Sequencing (Sanger Sequencing, Pyrosequencing) • High Resolution Melting Analysis (Real-Time PCR) Methylation Analysis: • Global and Gene-Specific (pyrosequencing) • Bisulfite Sanger Sequencing and Pyrosequencing Shared Instrumentation: • Agilent Bioanalyzer Lab-On-A-Chip, Beckman Biomek 3000 Liquid Handler, NanoDrop ND1000, NanoDrop 2000c, Stratagene Gradient Robocycler, ABI7900 384-well Real-Time Genetic Analyzer, Biotage PSQ96 MA, Affymetrix GeneChip System 3000, PCR Tamer, Thermocyclers CLIA-Certified Site: Operating under strict HIPAA regulations, the service core unit currently supports the Nemours Molecular Diagnostic DNA sequencing and fragment analysis CLIA-certified tests. Delaware Valley Network: The Nemours Biomolecular Core Laboratory provides backup services to other cores in the Delaware Valley Network including DNA sequencing coverage services for the University of Delaware DNA Sequencing and Genotyping Core. ---- Agilent Bioanalyzer Lab-On-A-Chip Beckman Biomek 3000 Liquid Handler NanoDrop ND1000, 2000c Stratagene Gradient Robocycler ABI7900 384-well Real-Time Genetic Analyzer ABI 3130xl Genetic Analyzer Biotage PSQ96 MA Affymetrix GeneChip System 3000 PCR Tamer Thermocyclers (2400, 9600, 9700) Computers (PC and MAC)

SD BRIN Proteomics Core Facility University of South Dakota 414 E Clark St Vermillion, SD 57069 (605) 677-5530 eduardo.callegari@usd.edu View Website Primary Contact: Eduardo Callegari (605) 677-5530 eduardo.callegari@usd.edu Last Updated: 10/18/2011	Services offered: 2d Spectra Acquisition and Interpretation Computational - Bioinformatics Data Analysis Mass Spectrometry Molecular Imaging Phosphor Imaging Proteomics Support Services - Shared Instrumentation Oversight & Maintenance Western Blot Associations: INBRE
The SD BRIN/USD Proteomics Core Facility provides state-of-the-art proteomics services to researchers from South Dakota and the region. Since 2002, the USD PCF has been providing proteomic analyses, as well as collaborating in the training of the use of common equipment such as the scanner, spot cutter, imaging software, technique and protocol issues, and sample preparation. Our goal is to provide all South Dakota researchers with the capability to rapidly analyze and identify protein expression patterns in their experimental systems. ---- 2D-nanoAcquity UPLC (Waters), Quadrupole Time of Flight Synapt G1 HD MS mass spectrmeter(Waters, Micromass), Proteome Works Spot cutter (Bio-Rad), Typhoon 9410 Multimage scanner (GE Healthcare), 2100 Bioanalyzer (Agilent Technologies), AKTA FPLC (GE Healthcare), SpeedVac centrifuge (Thermo), and SPE system with vacuum pump and manifold (Waters). Software’s: Mascot server (license for 2 PC), and Daemon Toolbox (Matrix Science), Mascot Distiller (Matrix Science), ProteinLynx Global Server v2.4 (Waters), PDQuest (Bio-Rad), and Proteo IQ (Nusep).

Genomics Core Facility University of Nevada, Las Vegas 4505 S. Maryland Parkway Las Vegas, NV 89074 (702) 895-1057 casey.hall@unlv.edu View Website Primary Contact: Casey Hall-Wheeler (702) 895-1057 casey.hall@unlv.edu Last Updated: 10/18/2011	Services offered: DNA Analysis Flow Cytometric Analysis Genomics Microarray Nucleic Acid Extraction RNA analysis RNA Integrity Sequencing - Capillary Sequencing Sequencing - DNA Sequencing Associations: INBRE
The UNLV Genomics Core Facility was established to aid in the development and support of genomics-based research, serving investigators in Nevada and beyond. Staff at the facility can be contracted for select services including ABI 3130 DNA sequencing, BD FACSCalibur flow cytometry, Affymetrix microarray processing and Agilent 2100 Bioanalyzer analysis. The facility also provides equipment and training for real-time PCR, Western blot/gel/microarray scanning, and analysis of DNA, RNA and protein samples. ---- ABI 3130 Genetic Analyzer BD FACSCalibur Flow Cytometer Affymetrix GeneChip Instrument System Agilent 2100 Bioanalyzer Typhoon 9410 Variable Mode Imager iCycler RT-PCR OmniGrid 100 Microarrayer GenePix 4000B Scanner NanoDrop SpectraMax Plus384 Spectrophotometer/Platereader Hyb4 Microarray Hybridization System Experion Automated Electrophoresis System Vibratome Cryostat Eppendorf Mastercycler Gradient Eppendorf Mastercycler Personal Applied Biosystems GeneAmp® PCR 9700 DNASTAR Lasergene v8.0 Software QIAGEN TissueLyser CentriVap Refrigerated Concentrator Beckman Coulter Optima L-90K Ultracentrifuge Eppendorf Centrifuge 5810 R Eppendorf Centrifuge 5424 CL-1000 UV Crosslinker DCode Universal Mutation Detection System Hybridization Oven 37˚C Incubator SDS PAGE and Western Blot Equipment 2D Gel Electrophoresis Equipment DNA Agarose Gel Equipment

Experimental Pathology Translational Research Core University of Vermont Burlington, VT 05405 (802) 656-3628 Mark.Evans@uvm.edu View Website Primary Contact: Mark Evans (802) 656-3628 mark.evans@uvm.edu Last Updated: 10/18/2011	Services offered: BioBanking F.I.S.H. Human Pathology Services - Diagnostics & Consultation Immunohistochemistry In Situ Hybridization Microscopy Nucleic Acid Extraction Tissue Microarray Associations: NERTRN Cancer Center CTSA
Our laboratory was established in 2000 to develop and support translational research projects within the Department of Pathology. Increasingly, we collaborate with the wider FAHC/UVM clinical and research community and occasionally with external institutions. The laboratory also has a central role in collecting, maintaining and distributing tissues for the VCC Tissue Banking Facility. Our lab assays include nucleic acids extraction from fixed and fresh pathology specimens, PCR techniques, in situ hybridization (ISH), immunohistochemistry (IHC), and tissue microarrays (TMA) for examining chromosomal changes and altered gene and protein expression patterns in pathology specimens. ---- Immunohistochemical autostainer In situ hybridization platforms Tissue microarray preparation

DartMouse Dartmouth Medical School 1 Medical Center Drive 610W Borwell, HB 7556 Lebanon, NH 03756 (603) 650-8686 dartmouse@dartmouth.edu View Website Primary Contact: Matthew Ranson (603) 650-8686 dartmouse@dartmouth.edu Last Updated: 10/18/2011	Services offered: DNA Analysis Genotyping Microarray SNP speed congenic and genetic background check Associations: Cancer Center COBRE
DartMouse™ is a not-for-profit core facility funded by the NIH's National Center for Research Resources. The mission of DartMouse is to facilitate the development of congenic mice in support of pre-clinical projects across the U.S. Use of DartMouse allows the generation of congenic mice in 5 generations (~1.5 year), vs. 10 generations (~3 years) required by conventional back-crossing. Investigators fill out our accession form and supply mouse tail clippings - from these DartMouse isolates genomic DNA and performs and analyzes complete genome-wide scans. We return data in graphical and spreadsheet formats, and make specific recommendations on breeder selection. In addition to helping investigators create congenic mice, we also provide a background check service to test the genetic purity of your mice. If there is contaminating genome present, we can then help you backcross onto your inbred strain of interest. Chips use a 1449 SNP array covering the mouse genome with an average density of < 2 cM. Turnaround time from receipt of samples to results is typically < 2 weeks. Prices are competitive. ---- Illumina BeadArray Reader QIA-xcel Nanodrop 2000 Promega Maxwell

Center for Bioinformatics and Computational Biology Core Facility University of Delaware Delaware Biotechnology Institute 15 Innovation Way; Suite 205 Newark, DE 19711 (302) 831-0161 polson@dbi.udel.edu View Website Primary Contact: Dr. Shawn Polson (302) 831-3235 polson@dbi.udel.edu Last Updated: 10/18/2011	Services offered: Computational - Bioinformatics Computational - High Performance Computing Data Analysis Associations: INBRE EPSCoR COBRE ABRF
The mission of the CBCB Core Facility is to provide scientific expertise and core infrastructure support in Bioinformatics and Computational Biology for the Delaware research and education community. The CBCB is under the direction of Dr. Cathy Wu, and the activities of the Bioinformatics Core are coordinated by Dr. Shawn Polson. Drawing on the combined resources of the CBCB, the Delaware Biotechnology Institute (DBI), and the Protein Information Resource (PIR), the Core provides both computing infrastructure and knowledgeable personnel with significant hardware, software and professional support for bioinformatics and computational needs. Four Research Faculty with broad expertise encompassing various areas of bioinformatics analysis provide both bioinformatics/computational services and/or research collaborations. The core staff also includes a Network and Information Systems Manager and a full-time IT associate. ---- A special focus of the Bioinformatics Core has been the expansion of next generation sequencing (NGS) analysis pipelines available as fee-for-service to support the growing number of investigators utilizing the advanced NGS capabilities of UD’s Genotyping and Sequencing Facility for genomic and transcriptomic-based discovery. The facility also has substantial capabilities built upon its close relationship with the Protein Information Resource (PIR) that have been successfully applied to a number of research projects including proteomic analysis using tools such as iProXpress and literature and data mining capabilities of iProLINK. These combined omic analysis and data integration pipelines form the nucleus of a broader bioinformatics framework that CBCB is actively developing to provide a user portal for dynamic analysis and visualization of genomic, transcriptomic, and proteomic data to its users at UD. ---- The Center’s in-house High-Performance Computational hardware includes over 500 processor cores supporting bioinformatic analysis. Included are approximately 200 cores freely accessible by researchers as part of our Linux-based Torque PBS cluster, BioHen. A combination of system types allows the choice of systems best suited for the particular needs of a given analysis. Included are numerous nodes configured for memory intensive computing applications with 128 - 512GB of RAM per machine. The data center currently hosts in excess of 100 TB of usable disk space. The CBCB also is a stakeholder in a campus-wide computer cluster, which will come online in fall of 2011 providing access to an additional 5000 processor cores. CLC Genomics Workbench, JMP, JMP genomics, Matlab, and a suite of other commercial and open source Bioinformatic and Statistical Software packages are supported and maintained on Center hardware. Our Database Cluster is composed of 6 Sunfire X4100M2 servers and acts as a repository of experimental data in relational databases. Both MySQL and Oracle database systems are available, allowing researchers to organize, store, and evaluate their data. Data security is a high priority and access to results other than through these methods is strictly limited. Our 3-D Visualization Studio is an immersive 3D graphics room with a 7'x15' rear-projection screen, delivering rear-projected, edge-blended images with total resolution of 2240 x 1024 pixels. Other resources and services include secure FTP server, file servers, on and off-site data backup servers, email server, streaming video server, web hosting, cloud-based storage interface, large format printing, administration of researcher-owned servers, and bioinformatics software license support.

Functional Genomics Center University of Rochester 211 Bailey Road CVRI West Henrietta, NY 14586 (585) 276-9997 michelle_zanche@urmc.rochester.edu View Website Primary Contact: Michelle Zanche (585) 276-9997 michelle_zanche@urmc.rochester.edu Last Updated: 10/18/2011	Services offered: Data Analysis DNA Analysis Genomics Genotyping Microarray Nucleic Acid Extraction Real-time qPCR RNA analysis RNA Integrity Sequencing - Capillary Sequencing Sequencing - DNA Sequencing Sequencing - Next-Gen Sequencing Associations: ABRF
The FGC provides core laboratory support, technical advice, and assistance with experimental design and data analysis for UR investigators in high-throughput sequencing, DNA sequencing, genotyping, gene expression, and RNA and DNA processing (extraction, purification, quantitation). ---- The following major equipment is located in the FGC: SOLiD 4 high throughput sequencer (Applied Biosystems), Illumina GAIIx high throughput sequencer, ABI 7900HT real-time PCR instrument with autoloader and microfluidic card module, ABI 3730 Capillary Electrophoresis DNA sequencer, Agilent 2100 Bioanalyzer, Covaris, CAS 1200 liquid handling robot, NanoDrop ND-1000 spectrophotometers, Tecan HS4800 hybridization station.

Neuroscience COBRE - Cellular and Molecular Biology Core University of Vermont 427 Health Science research Facility Burlington, VT 05405 (802) 656-2007 Sheryl.White@uvm.edu View Website Primary Contact: Sheryl White Last Updated: 10/18/2011	Services offered: 2D-PAGE Assays and Measurements Cell Culture Data Analysis Immunohistochemistry In Situ Hybridization Laser Capture Microdissection Library Services Mass Spectrometry Microscopy Nucleic Acid Extraction PCR Arrays Protein Extraction/Purification Proteomics Real-time qPCR RNA analysis SDS-PAGE Spectroscopy Tissue Culture Western Blot Associations: NERTRN COBRE
This facility, sponsored by the NCRR COBRE program, provides consulting expertise, training and many sophisticated pieces of equipment for performing molecular biology, proteomics, cell culture, histology and microscopy. The following major pieces of equipment are available in the core: Zeiss Palm Microlaser, Biotek Synergy H4 Plate Reader, Ciphergen SELDI-TOF, Countess Cell Counter, Microbrightfield Neuroleucida Morphometrics system, Odyssey Infrared Imager, BioRad Calligrapher microarray printer, Qiacube robots, BioRad Experion and much more. Use of the equipment is currently free for all users. ---- This core is available to all researchers at UVM, UVM affiliates and also researchers outside of UVM by prior agreement. Please contact us for further information.

Clinical Research Center UMass Medical School 55 Lake Ave North Worcester, MA 01655 (508) 856-2800 sheila.noone@umassmed.edu View Website Primary Contact: Sheila Noone (508) 856-2800 sheila.noone@umassmed.edu Last Updated: 10/18/2011	Services offered: Clinical Study Support and Resources Associations: NERTRN
Our new Clinical Research Suite within the CRC is dedicated to efficient, reliable and high-quality study support for clinical investigators. For a reasonable cost, investigators can utilize the following services as needed: Accessible and comfortable space for participants, just off the main lobby of ACC Six (6) exams rooms for study conduct Three infusion bays with comfortable recliners for lengthy visits Touchdown space for visiting researchers, their support staff and external monitors Conference room for start-up meetings, sponsor visits and research staff use In-unit laboratory equipped with centrifuge, hematocrit machine, -20 and -80 freezer space Prep area for packaging and shipping central lab specimens according to protocol requirements Regulatory support for IRB submissions; assistance with external regulatory communications; assistance with posting to http://www.clinicaltrials.gov

Transgenic Mouse Core New York University 540 First Avenue Skirball -Ground Floor New York, NY 10016 (201) 263-7942 MaryJean.Sunshine@med.nyu.edu View Website Primary Contact: Mary Jean Sunshine (212) 263-9074 maryjean.sunshine@med.nyu.edu Last Updated: 10/17/2011	Services offered: Assisted Reproductive Technologies (Rodent IVF, ICSI) Cell Culture Embryo Cryopreservation & Recovery Services Transgenics
The NYUMC Transgenic Mouse Core generates genetically modified mouse models for research via DNA injection or ES cell injection. Rederivation of all mouse strains imported to NYUMC is accomplished through conventional means as well as via utilizing imported fresh or cryopreserved embryos. We offer sperm and 8-cell embryo cryopreservation services to our users to save time, money and mouse space by freezing their valuable strains. We also offer IVF services using fresh or cryopreserved sperm samples. Frozen sperm and embryos can be imported from or exported to collaborators in lieu of sending live mice. We offer an ES cell targeting service utilizing any MAP-tested parental ES cell line requested by user as well as expansion of any MAP-tested targeted clone for blastocyst injection. We have experience expanding and injecting ES cell clones from multiple consortiums/repositories utilizing multiple mouse trains (C57Bl/6, Albino B6, Balb/C, etc.).

Human Embryonic and induced Pluripotent Stem Cell Facility Northwestern University 303 E. Superior Lurie, 10-232 Chicago, IL 60611 (312) 503-1039 l-lyass@northwestern.edu View Website Primary Contact: Ljuba Lyass (312) 503-1039 l-lyass@northwestern.edu Last Updated: 10/17/2011	Services offered: BioBanking Cell Culture Embryo Cryopreservation & Recovery Services Stem Cell Procurement Other, Sample Preparation, Specimen Preparation Facility Equipment, Training Associations: MWACD
This facility is providing human embryonic and induced pluripotent cell cultures that can be used directly in the experiments. The feeder-dependent and feeder-free systems are available. 2-day hands-on training sessions for 1 - 3 students per class are offered for the very affordable price. Super- clean and sterile, well-equipped space is offered for the investigates for culturing their stem cells. ---- Additional service: Training - technical hands-on classes ---- 2-day long hands-on class teaching basic methods of culturing human Embryonic and induced pluripotent Stem cells , Creating your own bank from a single vial purchased from the Stem Cell Bank (thawing, expanding and freezing of the needed amount of the vials), Culturing iPS cells derived by the investigator, culturing Human Embryonic Stem Cells on the feeder layer and in the feeder-free systems. Mouse irradiated feeder cells (live or frozen) Primary mouse fibroblasts. Providing the safe, sterile well-equipped space for the experiments with hES and iPS cells.

High Throughput Screening Laboratory University of Kansas, Lawrence 2034 Beckers Drive Structural Biology Center (SBC) Lawrence, KS 66047 (785) 864-1717 rathnam@ku.edu View Website Primary Contact: Rathnam Chaguturu (785) 864-1717 rathnam@ku.edu Last Updated: 10/17/2011	Services offered: Assays and Measurements Biochemical Analysis Cell Culture Cell Imaging Computational - Biostatistics Confocal Microscopy Data Analysis Flow Cytometric Analysis Surface Plasma Resonance (SPR) Tissue Culture High througput screening Associations: COBRE Cancer Center INBRE
High throughput screening of large chemical libraries of compounds is a proven way to identify novel chemical entities that target a biological system of interest. KU-HTS is a state- of-the-art facility dedicated to providing exceptional services in advancing drug discovery research initiatives, as well as assistance in preparing grant applications. HTS personnel have extensive experience in executing biochemical, cell-based, siRNA as well as high content screening campaigns against a plethora of target classes. KU-HTS is a fee-for-service facility dedicated to providing academia and nonprofit institutions, as well as the biotech and pharmaceutical industry, with exceptional quality services at the lowest cost structure. We partner with the investigators collaboratively to expedite their drug discovery efforts and serve as a uniquely qualified reference facility with high throughput screening instrument manufacturers as well as reagent kit developers and manufacturers.

University of Arizona Genetics Core Facility University of Arizona 1657 E Helen St. Rm 124 Univ of Arizona, Keating Bldg. Tucson, AZ 85721 (520) 621-9791 dnaseq@arl.arizona.edu View Website Primary Contact: Barbara Fransway (520) 626-1672 bbf@email.arizona.edu Last Updated: 10/17/2011	Services offered: BioBanking Data Analysis DNA Analysis Genomics Genotyping Nucleic Acid Extraction Outreach PCR Arrays Real-time qPCR RNA analysis Sequencing - Capillary Sequencing Sequencing - DNA Sequencing Sequencing - Next-Gen Sequencing Sequencing - Pyrosequencing Cell Line Authentication
UAGC provides a wide range of molecular biotechnology services and current molecular genetic methods. Fee-per-use services such as DNA Extraction, sample banking, transgenic mouse genotyping, Human Cell line Authentication, Sanger DNA Sequencing, Next-Generation sequencing platforms, Microsatellite DNA Typing, Sequenom multiplex SNP Genotyping and methylation analysis, Real-time PCR, and many others are available at a low cost to the entire research community. We specialize in custom workflows that couple our services with your specific research needs. ---- UAGC can design a custom workflow from our variety of services to address your specific research needs. We can take your tissue or cells and generate end-point data. UAGC has worked extensively with human ancestry platforms and large sample sizes and data sets. ---- UAGC houses the following equipment Qiagen Bio Sprint 96 DNA Extraction Robots (5 instruments) BioTek Microplate Florescence Readers (Synergy H1 and FLX800) ND-1000 Nano Drop Spectrophotometer (2 instruments) Biomek FX Liquid Handling Robots (3 instruments-genomic workflows) Biomek NX Liquid Handling Robot (1 instrument-genomic workflows) MJ thermocyclers (70 96-well cycling blocks) Biomek FX Liquid Handling Robots (1 instrument-Post-PCR workflows) Biomek NX Liquid Handling Robot (1 instrument-Post-PCR workflows) Agilent Bioanalyzer BioRad iCycler Real time PCR engine ABI 7300 Real-Time Instrument (2 instruments) ABI 7900 Real-Time Instrument Applied Biosystems 3730 DNA Analyzers (2 instruments-capillary sequencing workflows, 2 instruments- fragment analysis workflows) Sequenom MassArrayMALDI-TOF mass spectrometer RS1000 Nanodispenser robot Qiagen Tissue Lyser II Z1 Coulter Particle Counter TBS-380 Fluorometer Roche NimbleGen sequence capture array system Ion Torrent Personal Genome Machine Roche 454 FLX Illumina HiSeq 2000 Affymetrix Genechip Hybridization Oven Binder CO2 Incubator C150

Medicinal Chemistry Core University of Kansas, Lawrence 2034 Becker Drive Delbert M. Shankel Structural Biology Center (SBC) Lawrence, KS 66047 (785) 864-1611 cliu2@ku.edu View Website Primary Contact: Jeffrey Aube (785) 864-4496 jaube@ku.edu Last Updated: 10/17/2011	Services offered: Molecular Library Services Small molecule synthesis, libraries Associations: COBRE Cancer Center INBRE
The Synthetic Medicinal Chemistry Laboratory is a university-based drug discovery facility with a state-of-the-art infrastructure for pharmaceutical research. The Core provides medicinal, synthetic, and combinatorial chemistry support for Core B (HTS) and COBRE investigators. The support involves the optimization of compounds for potency, and selectivity by the following: Preparing targeted libraries for screening and lead optimization Synthesizing organic compounds on various scales (from mg to multi-gram) Assisting COBRE investigators and other university investigators, if requested, in the design of novel ligands and in the development of practical synthetic routes.

DNA and Protein Analysis and Cell Imaging Core Maine Medical Center Research Institute 81 Research Drive Scarborough, ME 04074 (207) 396-8148 varyc@mmc.org View Website Primary Contact: Calvin Vary (207) 396-8148 varyc@mmc.org Last Updated: 10/17/2011	Services offered: Cell Imaging Confocal Microscopy Mass Spectrometry Proteomics Sequencing - DNA Sequencing Associations: NERTRN COBRE
Standard dideoxy-based DNA sequence analysis, quantitative protein analysis via tandem msms using ICAT and related technologies; Quantitative protein analysis and multiple reagent monitoring using 4000QTRAP mass spectrometer. Confocal microscopy, four lasers, of living and fixed specimens. ---- Applied Biosystems DNA sequencers ABsciex QSTAR-Elite quadrupole TOF mass spectrometer ABsciex 4000QTRAP mass spectrometer Leica confocal microscope

Neuroscience COBRE Imaging and Physiology Core University of Vermont E015 Given 89 Beaumont Ave. Burlington, VT 05405 (802) 656-0413 tclason@uvm.edu View Website Primary Contact: Todd Clason (802) 656-0413 tclason@uvm.edu Last Updated: 10/17/2011	Services offered: Cell Imaging Computational - High Performance Computing Confocal Microscopy Data Analysis Microscopy Multiphoton Microscopy Optical Imaging Outreach Support Services - Shared Instrumentation Oversight & Maintenance Associations: NERTRN COBRE
The Neuroscience COBRE Imaging and Physiology Core provides a wide range of imaging techniques for fixed and live-cell immunofluorescence preparations. We maintain a Noran/Prairie Technologies LSCM (laser scanning confocal microscope), a DeltaVision RT restoration microscopy system, a Zeiss/BioRad dedicated multiphoton LSCM, a Nikon TIRF (total-internal reflection fluorescence) system, a Lamda DG-4 calcium ratiometric imaging system, a PTI monochromator system, an Andor iXon/Olympus widefield fluorescence microscope, and a Nikon SMZ-1500 fluorescence stereoscope. Patch-clamp electronics are available for use on most microscope systems, and each system has an integrated perfusion setup. ---- Noran laser scanning confocal microscope; DeltaVision Restoration microscopy system; BioRad/Zeiss dedicated multiphoton microscope; Nikon 488nm TIRF microscope; Nikon/Sutter ratiometric imaging system; PTI monochromator system with Evolve EMCCD; Andor EMCCD imaging workstation; Nikon fixed-stage imaging and physiology rig; Nikon stereo-dissection microscope with CCD.

Center for Translational Imaging (CTI) Northwestern University 710 North Fairbanks Court Olson Pavilion LC 0-225 Chicago, IL 60611 (312) 503-2198 mri-studies@northwestern.edu View Website Primary Contact: Todd Parrish (312) 926-2494 toddp@northwestern.edu Last Updated: 10/17/2011	Services offered: Functional Brain Imaging Magnetic Resonance Imaging (MRI) Nuclear Magnetic Resonance (NMR) Spectroscopy Computer Hardware/Software, Image Analysis, Imaging, Imaging Software, In vivo Animal Imaging , Spectroscopy, Training Associations: MWACD
CAMRI is the Department of Radiology's research imaging facility that is capable of high-end human and animal imaging with MRI, TMS, and EEG. CAMRI has the infrastructure and staff to support all types of imaging research studies. See cti.northwestern.edu for more information. ---- 1.5 Tesla whole body magnet - Siemens Espree, three 3 Tesla High field whole body MRIs - Siemens TIM TRIO (x2) and Siemens Skyra, 7T Small bore animal imaging magnet - Bruker ClinScan, TMS system - NexStim NBS system

DNA Sequencing Center Brigham Young University Dept of Biology, 401 WIDB Provo, UT 84602 (801) 422-3647 DNASC@byu,.edu View Website Primary Contact: Edward Wilcox (801) 422-3647 DNASC@byu.edu Last Updated: 10/17/2011	Services offered: DNA Analysis Genotyping RNA Integrity Sequencing - Capillary Sequencing Sequencing - DNA Sequencing Sequencing - Next-Gen Sequencing Sequencing - Pyrosequencing Associations: ABRF
We handle some 120,000 samples a year on the 3730xl sequencers. We performed 50 runs on the FLX in 2010, with more being projected for 2011. Turn around time is currently about 1 week or less on the 454. ---- 2 ABI 3730xl machines, 1 454 Life Sciences Genome Sequencer GS FLX+

Molecular Pathology Core of the COBRE Center for Cancer Res. Dev. Rhode Island Hospital Aldrich 611, 593 Eddy St. Providence, RI 02903 (401) 444-5849 lgantt@lifespan.org View Website Primary Contact: Laura Gantt (401) 444-8528 lgantt@lifespan.org Last Updated: 10/17/2011	Services offered: Assays and Measurements BioBanking DNA Analysis Histology Immunohistochemistry Laser Capture Microdissection Microscopy Nucleic Acid Extraction Optical Imaging PCR Arrays Real-time qPCR RNA analysis RNA Integrity Support Services - Shared Instrumentation Oversight & Maintenance Tissue microarray, Human tumor bank Associations: INBRE COBRE Cancer Center
Our core provides specialty immunohistochemical services, instrumentation, training and support personnel for the research efforts of the COBRE CCRD mentors and their junior associates as well as the academic community at large. We are located on the sixth floor of the Aldrich Building within the main campus of Rhode Island Hospital. Our equipment includes an ArcturusXT laser capture microdissector, Stratagene MX3005p realtime PCR instrument, BioRad iCycler, Agilent Bioanalyzer, Olympus fluorescence-capable microscope with image analysis software, Ventana Discovery immunohistochemistry processor, microtome, cryostat, Beecher tissue arrayer and Aperio ScanScope. Our tissue bank has 40 cu ft of ultra-low freezer space and provides human tumor tissue samples to all interested investigators who have received IRB approval for academic testing and research. ---- ArcturusXT laser capture microdissector, Stratagene MX3005p realtime PCR instrument, BioRad iCycler, Agilent Bioanalyzer, Olympus fluorescence-capable microscope with image analysis software, Ventana Discovery immunohistochemistry processor, microtome, cryostat, Beecher tissue arrayer and Aperio ScanScope.

Pathology Core Facility Northwestern University 710 North Fairbanks Court Olson Pavillion, Room 8421 ( 8412-8501) Chicago, IL 60611 (312) 908-5546 pathcore@northwestern.edu View Website Primary Contact: Piotr Kulesza (312) 908-5546 p-kulesza@northwestern.edu Last Updated: 10/17/2011	Services offered: Cytogenetics Histology Human Pathology Services - Diagnostics & Consultation Immunohistochemistry In Situ Hybridization Microscopy Nucleic Acid Extraction Protein Extraction/Purification Western Blot Characterization, Consultation, Cryo/Low-Temp Preparation, Data Storage/Off-Line Analysis, Deposition, Fluorescence/Photonic Microscopy, Image Analysis, Light Microscopy, Sample Preparation, Specimen Preparation, Specimen Preparation Facility Equipme Associations: Cancer Center MWACD
The Pathology Core Facility has three main components: research histology, specimen procurement/banking and protocol review/clinical trial support. The research histology component provides all of the tissue processing and histology services typically performed in a clinical laboratory but it is specifically dedicated to the needs of the Northwestern University research community in general and the Cancer Center research community in particular. ---- Clinical Trials Support Services - CLIA/CAP handling of bio-specimens in support of Pharmaceutical, Cooperative Group, and Investigator Initiated Clinical Trials on campus ( NMH , NMFF , RIC ) and off campus., Histology Services - Fixation , embedding , sectioning , histochemical staining and TMA ( Tissue Micro Array ), Immunochemical stain analysis ( qualification and semi-quantification ). Microscopy, Immunocytochemistry and immunohistochemistry, Molecular Laboratory -Large volume Clinical Trial sample extraction for nucleic acid and protein. ( FFPE , Blood , hair , nails , swab , cell ) CISH , FISH , TUNEL , western blot , ELISA , assay development, NATIONAL INSTITUTE OF HEALTH/NATIONAL CANCER INSTITUTE CORE LABORATORY FOR EASTERN COOPERATIVE ONCOLOGY GROUP - PATHOLOGY COORDINATING OFFICE AND REPOSITORY, Tissue Procurement and Banking for future research - Prostate Spore , Prostate Genetics , Tissue bank for all cancer samples - Liason to NMH Archival use - FFPE , serum , plasma , DNA , frozen tissue , cells

Applied Genomic Technologies Core Wadsworth Center -- New York State Department of Health Center for Medical Sciences/Biggs Laboratory Empire State Plaza, PO Box 509 Albany, NY 12201-0509 (518) 408-2137 gcfac@health.state.ny.us View Website Primary Contact: Patrick Van Roey (518) 474-1444 vanroey@wadsworth.org Last Updated: 10/17/2011	Services offered: Copy Number Variation (CNV) DNA Analysis Genomics Genotyping Microarray Nucleic Acid Extraction Sequencing - Capillary Sequencing Sequencing - DNA Sequencing Sequencing - Pyrosequencing gene copy number analysis
The Applied Genomic Technologies Core provides DNA sequencing, genotyping, and microarray services to research investigators. Below are brief descriptions and links to complete descriptions of all services provided by the core. The microarray services provided utilize Affymetrix GeneChips® or glass slide-based arrays. The laboratory processes and hybridizes samples for Affymetrix high-density oligonucleotide arrays (GeneChips) including expression arrays, resequencing arrays, tiling arrays and genotyping arrays. For glass slide-based approaches, samples are labeled and hybridized and scanned using an Agilent scanner. For further information, visit the Microarray home page or contact Zhen Zhang (518) 474-6992. Genotyping services include a variety of approaches for genotyping, as well as DNA purification and PCR troubleshooting/ optimization services. The types of genotyping analyses offered include Taq-Man-based single nucleotide polymorphism (SNP), fragment analysis and gene copy number variation (gene dosage) analysis using multiplex ligation-dependent probe amplification (MLPA). Human cell line verification by short tandem repeat (STR) testing is also available. For further information, visit the Genotyping home page or contact Melissa Leisner (518) 473-6606. DNA Sequencing can be performed on PCR products, plasmids, cosmids and BACs using ABI 3730XL and ABI3130XL instruments. A Biotage PyroMark ID Pyrosequencing System is also used for short-read sequencing and SNP genotyping. For further information, visit the Sequencing home page or contact Matthew Shudt (518) 474-4200.

Histology Core Maine Medical Center Research Institute 81 Research Dr. Scarborough, ME 04074 (207) 396-8151 mangog@mmc.org View Website Primary Contact: Grazina Armie Mangoba HTL (ASCP) (207) 396-8151 mangog@mmc.org Last Updated: 10/17/2011	Services offered: Histology Immunohistochemistry Tissue Processing, Sectioning and Staining Associations: NERTRN COBRE
The Histology Core facility was established to provide Histology services to the investigators associated with the Center of Biomedical Research Excellence (COBRE) in Regenerative Medicine. The objective is to provide high quality stained tissue sections on microscope slides to support the research at the institute. Services include tissue fixation, processing, embedding, sectioning of frozen and paraffin embedded tissue and staining. Staining ranges from routine H&E’s to special stains demonstrating specific tissue structures. Immunohistochemical staining is offered for a general selection of antibodies.

Protein Facility protein@iastate.edu 1178 MBB Ames, IA 50011 (515) 294-3267 protein@iastate.edu View Website Primary Contact: Joel Nott (515) 294-3267 protein@iastate.edu Last Updated: 10/17/2011	Services offered: 2D-PAGE Mass Spectrometry Peptide Synthesis Phosphor Imaging Protein Extraction/Purification Proteomics SDS-PAGE Sequencing - Protein Sequencing Surface Plasma Resonance (SPR) Western Blot circular dichroism, HPLC Associations: ABRF
The Protein Facility of the Iowa State University Office of Biotechnology is open to faculty and students from the university, other educational institutions, and industry scientists. The facility offers protein/peptide sequencing, large and small scale peptide synthesis (Fmoc), matrix-assisted laser desorption/ionization (MALDI) mass spectrometry, circular dichroism (CD), SDS-PAGE/electroblotting, 2-D gel electrophoresis, isoelectric focusing (IEF), in-gel digestion/peptide mass fingerprinting, tandem mass spectrometry (LC-MS, LC-MS/MS and LC/LC-MS/MS), digital image acquisition and analysis using the Typhoon imaging system and the 2D gel documentation/analysis system, and preparative, analytical and micro-analytical high performance liquid chromatography (HPLC) MALDI, HPLC, SDS-PAGE/blotting, IEF, 2D gel electrophoresis, the Biacore T100, 2D gel documentation/analysis, the Typhoon imaging system and CD are all available for user operation after appropriate training. ---- Amersham-Pharmacia IPGPhor IEF unit Amersham Pharmacia DALT 2D electrophoresis system Amersham Pharmacia Image Scanner Applied Biosystems Model 494 Procise protein/peptide sequencer Applied Biosystems DE-Pro MALDI mass analyzer Applied Biosystems Q-Star XL quadrapole-TOF tandem mass spectrometer with attached LC-Packings UltiMate capillary HPLC (shared instrument located in the W.M. Keck Metabolomics Research Laboratory) Three Beckman System Gold high-performance liquid chromatographs Model 125A Analytical Solvent Module with Model 166 programmable UV detector Model 110A Semi-prep/prep Solvent Module with Model 166 programmable UV detector Model 125S Microanalytical Solvent Module with Model 166 programmable UV detector and SC100 fraction collector Beckman Coulter P/ACE MDQ Capillary Electrophoresis System Biacore T100 Surface Plasmon Resonance Instrument Biorad minigel and blotting apparatus C.B.S. Scientific spot-picking workstation with UV lightbox Genomics Solutions ProGest Jasco J-710 Spectropolarimeter Molecular Dynamics Typhoon 9410 NanoDrop ND1000 Spectrophotometer Savant SpeedVac Plus Virtis Model 3.5L DBTZL Benchtop FreezeDryer (lyophilizer)

Molecular Bioinformatics Shared Resource University of Vermont D-207 Given Building Burlington, VT 05405 (802) 656-4608 Julie.Dragon@uvm.edu View Website Last Updated: 10/17/2011	Services offered: Computational - Biostatistics Associations: NERTRN CTSA COBRE Cancer Center INBRE
Personnel: Jeff Bond and Julie Dragon Services: Bioinformatics, genomic expression analysis

Biostatistics University of Vermont 27 Hills Building Burlington, VT 05405 (802) 656-2526 Takamaru.Ashikaga@uvm.edu View Website Primary Contact: Takamaru Ashikaga (802) 656-2526 Takamaru.Ashikaga@uvm.edu Last Updated: 10/16/2011	Services offered: Computational - Biostatistics Data Analysis Associations: NERTRN Cancer Center CTSA
The VCC Biostatistics Shared Resource provides consultative and collaborative support to VCC investigators in the general areas of study design, data collection and quality control, data processing, data management, statistical methods development, statistical analysis and interpretation, and manuscript preparation. ---- Other capabilities include epidemiology study design as well as the conduct and analysis of data; program evaluations of biomedical clinical trials, health promotion interventions and telemedicine systems; population based primary data collection using computer aided telephone interviewing (CATI)methods, and Interactive Voice Response (IVR) telephone applications. ---- The Facility has computing capabilities on multiple platforms including WINDOWS, MAC, LINUX, UNIX, and DOS. These systems can be used for statistical programming and analysis via standard statistical software such as SAS, BMDP, SPSS, Stata, SAGE (Statistical Analysis for Genetic Epidemiology), S-PLUS, SUDAAN, OCMAP, HML, and EGRET (software for statistical analysis of epidemiological data).The Unit also includes a Novell LAN, 12-station Computer Assisted Telephone Interviewing (CATI) system with primary and back-up servers.

Protein Expression Laboratory National Cancer Institute at Frederick SAIC/Frederick, Inc. PO Box B, 327-7 Frederick, MD 21702 (301) 846-7376 espositod@mail.nih.gov View Website Primary Contact: Dominic Esposito (301) 846-7376 espositod@mail.nih.gov Last Updated: 10/16/2011	Services offered: Assays and Measurements Cell Culture Gel Chromatography Molecular Construct Services Monoclonal Antibody Nucleic Acid Extraction PCR Arrays Protein Extraction/Purification Proteomics Real-time qPCR RNA Integrity SDS-PAGE Sequencing - Protein Sequencing Tissue Culture Transgenics Viral Vectors Virology Western Blot Associations: ABRF Cancer Center
The PEL comprises six service groups. Our services are available to all NIH intramural researchers, and by interagency agreements to FDA and US Army at Ft. Detrick. We are happy to consult and distribute materials to not-for-profit institutions. Clone Optimization: DNA cloning for protein expression; Gateway-based; fluorescent, epitope, solubility, purification tags; mutagenesis; >200 vectors for insect/ baculovirus, mammalian, E. coli, P. pastoris, K. lactis Microbial Expression: E. coli, P. pastoris, K. lactis, 2 mL to 60 L, autoinduction and IPTG; fully instrumented fermenters, 6 x 2L, 4 x 20L, 1 x 80L Eukaryotic Expression: insect / baculovirus, mammalian transient and stable, monoclonal antibodies Protein Purification: parallel microscale for process development; affinity and native, tag and endotoxin removal; scale-up to low gram scale Virus Technology: custom and stock adenovirus and lentivirus production; titer determination; promoter and reporter panels in lentivirus ready to deliver Molecular Detection: qPCR and ELISA assays developed and executed, focused on human viruses in human samples; qPCR assays for mycoplasma and human pathogenic viruses contaminating cell lines; cell line identity testing ---- Each PEL service is available individually or as part of a larger project.

DNA Core Facility University of Missouri Bond Life Science Center, room 216 1201 Rollins Columbia, MO 65211 (573) 882-0428 dna-core@missouri.edu View Website Primary Contact: Nathan Bivens (573) 882-0428 bivensn@missouri.edu Last Updated: 10/15/2011	Services offered: DNA Analysis Genotyping Microarray RNA analysis RNA Integrity Sequencing - Capillary Sequencing Sequencing - DNA Sequencing Sequencing - Next-Gen Sequencing Associations: MWACD ABRF
The DNA Core Facility is a state of the art research facility providing services to investigators on all four campus of the University of Missouri system and to investigators of outside academic institutions and commercial enterprises. Our research facility provides services in DNA sequencing, DNA fragment analysis, genotyping, and gene expression profiling. The core currently provides these services to over 150 researchers representing 7 colleges and 50 departments in the MU system and to investigators of outside academic institutions and commercial enterprises. Sanger DNA sequencing and fragment analysis services accommodate both single sample users and high-throughput projects. The facility utilizes a 3730 96-capillary DNA Analyzer which provides high throughput capacity at minimal cost to investigators. DNA sequencing is performed with Applied Biosystems Big Dye Terminator cycle sequencing chemistry providing accurate 800-900 bases of sequence. In addition, the 3730 DNA Analyzer is used for size determination on DNA fragments, such as, microsatellites (random 2-6 base pair repeats) and AFLPs (amplified fragment length polymorphisms), providing a robust and high throughput method for fragment analysis techniques. Next-Generation sequencing technologies are supported with the Illumina Genome Analyzer II (GAII) and Illumina HiSeq 2000. These ultra high-throughput DNA sequencing platforms are capable of generating billions of bases of sequence data in a single run. The HiSeq 2000dramatically improves the speed, efficiency, and cost-effectiveness of current genetic analysis platforms. Currently available applications are genome sequencing , genome-wide SNP analysis, whole transcriptome profiling, small RNA discovery/profiling, and chromatin immunoprecipitation sequencing. The Affymetrix system is a fully integrated platform for analysis of Affymetrix application-specific oligonucleotide arrays. The technology allows investigators to monitor and compare genome-wide changes in gene expression. The system includes a GCS3000 scanner and FS450U automated fluidics station. Services include preparation of biotinylated cRNA target, hybridization of the cRNA target to the DNA chip, washing, staining and scanning. ---- Illumina HiSeq 2000 Applied Biosystems 3730xl Affymetrix FS450U Fluidics station and GCS3000 Scanner Agilent BioAnalyzer 2100 BioRad Experion Tecan Freedom Evo 100 Diagenode BioRuptor

Human Immune Monitoring Center Stanford University Fairchild Science Building, Room D033 299 Campus Drive Stanford, CA 94305 (650) 723-4984 maecker@stanford.edu View Website Primary Contact: Holden Maecker (650) 723-1671 maecker@stanford.edu Last Updated: 10/15/2011	Services offered: Assays and Measurements Flow Cytometric Analysis Nucleic Acid Extraction Real-time qPCR RNA analysis RNA Integrity Associations: Cancer Center CTSA
The Human Immune Monitoring Center (HIMC) is designed to be a comprehensive source for immune monitoring services for clinical and translational studies. Goals of the HIMC include: -To provide standardized, state-of-the art immune monitoring assays at the RNA, protein, and cellular level. -To test and develop new technologies for immune monitoring. -To efficiently archive, report, and mine data from immune monitoring studies, so as to increase the value of the data and to assist in biomarker discovery. The HIMC uses an online database for integration of data from the standard HIMC assays, along with de-identified clinical and demographic data. ---- CyTOF mass cytometry assays are being developed. ---- Luminex, ELISPOT, CyTOF

Instrumentation and Model Facility University of Vermont 280 East Avenue ST2 Burlington, VT 05405 (802) 656-2976 info@its.uvm.edu View Website Primary Contact: Tobey Clark (802) 656-0069 tobey.clark@uvm.edu Last Updated: 10/15/2011	Services offered: Fabrication Optical Imaging Support Services - Audiovisual Support Support Services - Electronics & Fabrication Shop Support Services - Shared Instrumentation Oversight & Maintenance custom instrumentation design, physical modeling, parts Associations: NERTRN EPSCoR
For over forty years, the Instrumentation & Model Facility (IMF) has been the University of Vermont's centralized source for custom instrumentation design, fabrication, and 3D modeling. We provide creative, high quality, cost effective, and timely services and products for researchers, students, educators, other universities, state government, healthcare institutions, and innovative companies enhancing Vermont’s economic future. IMF engineers employ technical expertise in mechanical, materials, electrical, optical, and programming disciplines to solve application problems for customers. We have a complete machine shop with CADD/CAM and can work with a variety of materials, Objet Polyjet 3D printing, and a electronics/computer development laboratory. This service is available at reasonable rates with estimates provided if requested. ---- Complete machine shop; CADD/CAM; Objet 3D printing up to 14x14x8; welding;electronics/computer design and fabrication systems; optical design;

Small Animal Imaging Core UMass Medical School 55 Lake Ave North SA-107A Worcester, MA 01655 (508) 856-4256 Donald.Hnatowich@umassmed.edu View Website Primary Contact: Mary Rusckowski (508) 856-6972 Mary.Rusckowski@umassmed.edu Last Updated: 10/15/2011	Services offered: Functional Brain Imaging Molecular Imaging Small animal SPECT, CT and Pet Imaging Associations: NERTRN
With the installation of both cameras, the Bioscan NanoSPECT/CT and the Philip Mosaic PET small animal cameras, UMMS and extramural investigators now have the opportunity of using radioactive agents labeled with SPECT radionuclides such as 99mTc, 111In ,123I 125I and PET radionuclides such as 18F, 64Cu, 68Ga, to follow the pharmacokinetics and biodistributions in mice, rats, rabbits, cats and marmoset monkeys and other small animals of proteins, peptides, oligomers, nanoparticles, and other biomarkers of interest. The CT component of the NanoSPECT camera will provide registration for both cameras and, in addition, can be used without radioactivity for high resolution anatomical imaging. ---- The UMMS Small Animal Imaging Core Facility is now operational and will serve the UMMS and extramural research community by providing state-of-the-art single photon computerized tomography (SPECT), positron emission computerized tomography (PET) and X-Ray computerized tomography (CT) for imaging small animals. The biodistributions of radiolabeled biomarkers of interest such as proteins, peptides, oligomers, nanoparticles, etc. may be followed over time in small animals noninvasively using the Bioscan NanoSPECT/CT camera with single photon radionuclides such as 99mTc, 111In, 123I, 125I and/or with the Philip Mosaic PET camera with positron emitting radionuclides such as 18F, 68Ga, and 64Cu. The accompanying software allows for the accurate quantitation of radioactivity in organs and lesions. The CT component of the NanoSPECT camera will provide registration for both cameras and, in addition, can be used without radioactivity for high resolution anatomical imaging. Please visit our website for additional details. http://umassmed.edu/saicf/index.aspx The staff and faculty of the Small Animal Imaging Core Facility are committed to providing complete small animal radioactivity imaging capability to researchers within and without the UMMS system. For the present, this includes consultation services to assist researchers in designing imaging studies that are appropriate for their goals, limited radiolabeling services and data acquisition and analysis. In the future, we intend to expand our radiolabeling services to better assist those researchers without the expertise or the interest in radiolabeling of biomarkers. We also intend to expand our data analysis capabilities to provide researchers with more comprehensive final reports adjusted to their needs.

BIDMC Mass Spectrometry Core Beth Israel Deaconess Medical Center 3 Blackfan Circle, CLS-409 Beth Israel Deaconess Medical Center Boston, MA 02115 (617) 735-2651 jasara@bidmc.harvard.edu View Website Primary Contact: John Asara (617) 735-2651 jasara@bidmc.harvard.edu Last Updated: 10/15/2011	Services offered: Computational - Bioinformatics Data Analysis Mass Spectrometry Proteomics Sequencing - Capillary Sequencing Sequencing - Protein Sequencing Support Services - Shared Instrumentation Oversight & Maintenance Metabolomics Associations: ABRF Cancer Center
The mass spectrometry, metabolomics and proteomics core at Beth Israel Deaconess Medical Center was established in 2004 led by Dr. John Asara and continues to grow rapidly. We provide excellent proteomics and metabolomics services with low prices and fast turnaround times. We have cutting edge equipment operated at optimal sensitivity and work with world class researchers. We also mainatin an active internal cancer proteomics research program with collaborative efforts. Our expertise is in identifying and quantifying protein modifications and dynamic protein-protein interactions in addition to metabolomics profiling analyses. We look forward to helping you and progressing your research to the next level. ---- Label-free Quantification Protein-protein interaction networks Phosphorylation site mapping ---- AB/SCIEX 5500 QTRAP LTQ Orbitrap XL-ETD LTQ linear ion trap Orbitrap Velos Pro

Proteomics & Mass Spectrometry Facility Donald Danforth Plant Science Center 975 North Warson Rd St. Louis, MO 63132 (314) 587-1415 salvarez@danforthcenter.org View Website Primary Contact: Sophie Alvarez (314) 587-1415 salvarez@danforthcenter.org Last Updated: 10/15/2011	Services offered: 2D-PAGE Data Analysis Mass Spectrometry Protein Extraction/Purification Proteomics SDS-PAGE Sequencing - Protein Sequencing Amino Acid Analysis, Targeted Metabolite Analysis Associations: MWACD ABRF
The Danforth Center’s Proteomics & Mass Spectrometry Facility develops new tools and provides high quality sample analysis in proteomics, mass spectrometry and related analytical fields. For info on capabilities and instrumentation - please visit: www.danforthcenter.org/pmsf ---- plant hormone analysis

Immune Monitoring and Flow Cytometry Shared Resource Dartmouth Medical School 722 Rubin Builing 3W Borwell Building Lebanon, NH 03756 (603) 653-9913 jacqueline.smith@dartmouth.edu View Website Primary Contact: Alan Bergeron (603) 653-9913 alan.bergeron@dartmouth.edu Last Updated: 10/15/2011	Services offered: Assays and Measurements Cell Culture Cell Sorting Data Analysis Flow Cytometric Analysis Tissue Culture Immune Monitoring Associations: EPSCoR COBRE Cancer Center INBRE
We carry out mechanistic studies using human PBMCs for clinical trials; ELISPOT, multiplex cytokine analysis, multi-color cell phenotyping; blood processing and cryopreservation. We also carry out up to 10-color flow cytometry and up to 11-color FACS sorting. ---- Gallios, MACSQuant, FACSAria, Canto, FACSCalibur, FACScan flow cytometers. autoMACS, Robosep automated magnetic bead separation. Sector(MSE)and Bio-Plex multiplex cytokine analysers. Zeiss KS ELISPOT reader.

Clinical Research Management University of Vermont Courtyard at Given - 4 North 89 Beaumont Avenue Burlington, VT 05405 (802) 656-4414 Karen.Wilson@uvm.edu View Website Primary Contact: Karen Wilson Last Updated: 10/14/2011	Services offered: Protocol Development/Clinical Trial Coordination Clinical Reserach Manaagment Associations: NERTRN Cancer Center
The Clinical Research Management Core enhances clinical research by monitoring the participation process, providing centralized data management oversight, and by working closely with investigators during the development analysis prior to publication. The Clinical Research Management Core is in essence the 'operations office' for all clinical trials conducted by VCC investigators.

The Genomics Core Facility The Pennsylvania State University The Genomics Core Facility 407 Chandlee Building University Park, PA 16802 (814) 865-3332 dsg4@psu.edu View Website Primary Contact: Dr. Deborah Grove (814) 865-3332 dsg4@psu.edu Last Updated: 10/14/2011	Services offered: Genomics Genotyping Microarray oligo synthesis Real-time qPCR RNA Integrity Sequencing - Capillary Sequencing Sequencing - DNA Sequencing Sequencing - Next-Gen Sequencing SNPs by Open Array Associations: ABRF
The Facility offers DNA services including Sanger and Next-Gen sequencing by ION PGM, 454 and SOLiD, genotyping by VNTRs and SNP including Open Array, real-time qPCR, and DNA synthesis. RNA services include Microarray and Next-Gen applications such as Transcriptome, Small RNA and Degradome.

Rochester Human Immunology Center Core Laboratory Unoversity of Rochester David H. Smith Center for Vaccine Biology and Immunology 601 Elmwood Ave., P.O. Box 609 Rochester, NY 14642 (585) 273-4473 Sally_Quataert@urmc.rochester.edu View Website Primary Contact: Sally A. Quataert (585) 273-2454 Sally_Quataert@urmc.rochester.edu Last Updated: 10/14/2011	Services offered: Assays and Measurements Cell Culture Cell Imaging Cell Sorting Computational - Application Development Flow Cytometric Analysis Outreach Protocol Development/Clinical Trial Coordination Tissue Culture Associations: ABRF CTSA ISAC
Modern immunological techniques are sophisticated and powerful, and can be applied effectively to many basic and clinical projects. However, it is difficult for any one investigator to become expert in all of these complex techniques and expertise in immunology is typically spread across different Departments. The Human Immunology Center (HIC) within the David H. Smith Center for Vaccine Biology and Immunology has been established to acquire, refine and develop expertise in cutting-edge techniques and to support applications in Human Immunology research. The HIC is designed with capabilities to enhance multi-disciplinary research initiatives catalyzing key clinical and basic immunology research in vaccines, HIV/AIDS, autoimmunity, allergy/asthma, transplantation as well as cancer immunology. ---- method validation ---- BD LSR IIs, BD FACS ARIAs, BD FACS CAntos, Luminex 200, CTL Immunospot reader, Blood and specimen processing facility, cryofreezers, BSL2+ facility

Flow Cytometry Core Facility University of Connecticut Health Center 263 Farmington Ave. Room L5058 Farmington, CT 06030 (860) 679-3463 jellison@uchc.edu View Website Primary Contact: Evan R. Jellsion, Ph.D. (860) 679-6595 jellison@uchc.edu Last Updated: 10/14/2011	Services offered: Cell Sorting Flow Cytometric Analysis Multiplex Associations: NERTRN
State of the art flow cytometry core facility housing 3 BD LSR II and 2 BD FACS Calibur instruments for cellular analysis along with 2 BD FACS ARIA II cell sorters. We also have the capacity to sort unfixed biohazardous samples using the Baker BioProtect III safety cabinet. http://flowcytometry.uchc.edu ---- LSR II 4 laser 7/2/2/3 LSR II 4 laser 6/2/2/3 LSR II 5 Laser 7/2/5/2/3 Aria II 5 laser 3/3/5/2/4 Aria II 5 laser 7/3/2/2/2 BioProtect III hood

Life Science Informatics University of Alaska 909 Koyukuk Drive, Room 222 PO Box 757280 Fairbanks, AK 99775 (907) 474-5768 biotech@alaska.edu View Website Primary Contact: Shawn Houston (907) 474-5768 houston@alaska.edu Last Updated: 10/14/2011	Services offered: Computational - Application Development Computational - Bioinformatics Computational - Biostatistics Computational - High Performance Computing Data Analysis Outreach Data Management Associations: INBRE EPSCoR
The Life Science Informatics Core cyberinfrastructure includes two computing clusters, a large memory computational server, a database server, and a file server. All of the Core servers are housed in University of Alaska Butrovich Computer Data Center. The Data Center provides uninterrupted power systems (UPS), room environmental monitoring, fire alarm/suppression systems, and card access and video security systems. A 328 CPU core (64-bit Linux) IBM system hosts the Bioinformatics Computational Portal. This cluster provides accessible computations: users without the knowledge of Unix can submit computational jobs through a web-based interface. The Portal was designed to meet the needs of researchers at University of Alaska. The Computational Portal has over 100 applications in the following categories: similarity and homology, sequence analysis, population genetics, phylogenetic analysis, and miscellaneous utilities. Although the most current applications are for genomics, we generally install and attach the web interface to any life-science software requested. The Portal software is flexible enough to create web-based data-analysis pipelines. In addition, educators can use the Portal for undergraduate training: students can experience first-hand genomic analysis through a web interface that is not intimidating. The 2-node Sun Microsystems (Oracle) Cluster (64-bit) is used as a login cluster. Parallelized custom work flows have been created for investigators to run on the login cluster. Users who are more comfortable with a command-line interface, who write their own scripts and install or write their own analysis programs use the login cluster. The Core also provides an infrastructure for collaboration among researchers that enables dissemination of data and information collected by researchers. The Secure File Share (SFS) allows researchers to securely share documents and data with their collaborators, both within and outside of the United States. The SFS allows users on different architectures (PCs, Macs, and Linux computers) to seamlessly share files through an easy drag and drop interface. Users can also request a locking file share that requires that multiple users check out documents in order to facilitate editing of the documents. The Life Science Informatics Core maintains and designs web pages to share the data collected by University of Alaska investigators.

Stanford Shared FACS Facility Stanford University 279 Campus Drive West Beckman B015 Stanford, CA 94305 (415) 723-6959 facs-inquiries@mailman.stanford.edu View Website Primary Contact: Marty Bigos (415) 723-6959 facs-inquiries@mailman.stanford.edu Last Updated: 10/14/2011	Services offered: Cell Sorting Flow Cytometric Analysis Associations: Cancer Center ISAC
The SSFF provides the following services on a recharge basis to the research community: o Cell sorting and analysis of BSL-1 and BSL-2 samples, either with or without operator support. o In-depth training for researchers who want to operate the instruments themselves. o Consulting with researchers on design and data analysis of flow cytometry experiments. o Maintaining a site license for FlowJo software (originally developed in the SSFF) for the University at large, providing a 65% savings to the Stanford community. o Data management services, including on and off-site backups, of all data generated in the Facility for the past 10 years. o Off-site maintenance and training services for Stanford research groups whose projects require dedicated instrumentation. In addition the SSFF stays abreast of, and tests, new applications and reagents, and makes the results available to its user base, either through postings or a series of sponsored lectures and seminars.

UTMDACC Proteomics Facility UT-MDAnderson Cancer Center PO Box 301429 unit 951 Houston, TX 77230 (713) 834-6096 dhawke@mdanderson.org View Website Primary Contact: David Hawke (713) 834-6096 dhawke@mdanderson.org Last Updated: 10/14/2011	Services offered: Mass Spectrometry Proteomics Associations: Cancer Center ABRF
Proteomics - MW, Protein ID, PTMs, Stable Isotopes, NEW! Quant by QQQ! ---- LTQ-Orbitrap, LTQ with NanoLC and ETD, 4700 TofTof, Agilent 6460 Tandem Quadrupole.

Cell Screening Core Weill Cornell Medical College 1300 York Avenue, Rm E-00.05 New York, NY 10065 (212) 746-6480 har2001@med.cornell.edu View Website Primary Contact: Harold (212) 746-6480 har2001@med.cornell.edu Last Updated: 10/14/2011	Services offered: Cell Imaging Data Analysis Optical Imaging
The cell screening core provides equipment and expertise to assist investigators in screening chemical compound libraries for drug discovery and siRNA libraries for gene knockdown using cell-based assays, automated fluorescence microscopy, digital image processing and data mining software. ---- ImageXpress Micro from Molecular Devices equipped with a ThermoCRS Catalyst Express robotic plate loader. MetaXpress software for data acquisition and analysis.

Gene Targeting and Transgenic Facility Roswell Park Cancer Institute Elm and Carlton Sts. MRC 312A Buffalo, NY 14263 (716) 845-5843 aimee.stablewski@roswellpark.org View Website Primary Contact: Aimee Stablewski (716) 845-5843 aimee.stablewski@roswellpark.org Last Updated: 10/14/2011	Services offered: Assisted Reproductive Technologies (Rodent IVF, ICSI) Cell Culture Embryo Cryopreservation & Recovery Services Stem Cell Procurement Tissue Culture Transgenics Associations: Cancer Center
The Gene Targeting and Transgenic Facility at Roswell Park provides investigators in the Western New York area with genetically modified animals for their research through gene targeting and transgenic methodologies. ---- We provide generation of transgenics via pronuclear microinjection and targeted mutants via blastocyst microinjection. The facility also provides additional assisted reproductive services such as in vitro fertilization, sperm and embryo cryopreservation as well as derviation of ES cell lines.

UMass Cancer Center Tissue and Tumor Bank Core UMass Medical School 364 Plantation Street LRB 470U Worcester, MA 01605 (508) 856-4774 stephen.lyle@umassmed.edu View Website Primary Contact: Cherie Taglienti (508) 856-4432 cherie.taglienti@umassmed.edu Last Updated: 10/14/2011	Services offered: BioBanking Cell Culture Histology Human Pathology Services - Diagnostics & Consultation Laser Capture Microdissection Microscopy Real-time qPCR Tissue Culture Xenograft / Xenotransplantation Human tissue samples, Frozen Sections, Formalin-fixed tissue sections Associations: NERTRN Cancer Center
The UMass Cancer Center Tissue and Tumor Bank is an institutional core facility aimed at improving the research endeavors of the basic, clinical, and translational cancer scientists. The objective of the Tissue Bank is to systematically collect, store and distribute human tissues to researchers to support research projects dedicated to improving the understanding, care, and treatment of individuals afflicted by cancer. One vital component of the Tissue Bank is the archival collection of formalin-fixed, paraffin embedded tissue samples that can be connected to clinical information dating back to 1993. The second major component of the Tissue Bank is a dynamic tissue collection- excess surgical specimens are collected fresh and routinely snap frozen, frozen in OCT, and formalin fixed, paraffin embedded. Anonymous, de-identified clinical and pathologic data are linked to the specimens in a secure database. Other disease tissues are available upon request and specific study needs can be met. In addition to tissue collection, bone marrow and blood specimens are collected in conjunction with a clinical trial established by Jan Cerny, MD. New in 2011- ascites fluid is collected from cancer patients. Cultured ovarian and endometrial cells are available for request as well as fluid specimens from breast and lung cancer. ---- Bone marrow samples (fresh, frozen) Blood samples (fresh, frozen) Ascites from cancer patients (fresh, frozen) Cultured ovarian and endometrial cancer cells from ascites

Molecular Morphology and Imaging Core Medical University of South Carolina Dept. of Regenerative Medicine and Cell Biology 173 Ashley Avenue, BSB644 Charleston, SC 29425 (843) 792-3756 truskt@musc.edu View Website Primary Contact: Thomas Trusk (843) 792-3756 truskt@musc.edu Last Updated: 10/14/2011	Services offered: Cell Imaging Computational - Biostatistics Confocal Microscopy Data Analysis Histology Immunohistochemistry In Situ Hybridization Microscopy Support Services - Audiovisual Support Support Services - Graphic Services Support Services - Print Shop/Document Center Associations: EPSCoR COBRE
This core provides the equipment and expertise necessary for research requiring histological methods, advanced microscopy and image analysis. Histological services include materials for frozen or paraffin sectioning and staining, and support for immunohistochemistry and in situ hybridization. The microscope core operates a Zeiss Axio Imager M2, a Perkin-Elmer UltraVIEW spinning disc confocal, a Leica SP2 confocal, and a Leica SP5 confocal. Several workstations support image analysis, 3D reconstruction and statistical support. The core provides workstations for gel documentation and x-ray film processing. Core personnel are available for computer support and provide printing services for local users.

IBEST Computational Resources Core University of Idaho, IBEST Dept. of Biological Sciences attn: James A Foster Moscow, ID 838443051 (208) 885-7062 foster@uidaho.edu View Website Primary Contact: James A Foster (208) 885-7062 foster@uidaho.edu Last Updated: 10/14/2011	Services offered: Computational - Bioinformatics Computational - Biostatistics Data Analysis DNA Analysis Associations: NICL INBRE EPSCoR COBRE ABRF
IBEST Bioinformatics Core – Four compute clusters: 512 core AMD64 primary production system; 96 processor Intel Xeon overflow system; 44 processor Intel Xeon development system; 32 core system dedicated to the post processing of sequencing data from our 454. High end stand-alone servers for non-parallel or non-array projects. Data Storage: 228TB gross HDD storage capacity; 60TB tape backup; Our storage is divided into two tiers: Firstly high performance local storage for our clusters and stand-alone systems and secondly, high capacity commodity storage acting as a longer term data repository. All systems are housed in a data center on the University of Idaho campus specifically designed for high performance computing. Staffing includes two full time systems administrators and two PhD level scientists to help users. ---- Bioinformatics training, and bioinformatics workbooks for the classroom

WVU Flow Cytometry Core Facility West Virginia University HSCN Room 2160 PO Box 9177 Morgantown, WV 26506-9177 (304) 293-6273 flowcore@hsc.wvu.edu View Website Primary Contact: Kathleen Brundage, Ph.D. (304) 293-6273 kbrundage@hsc.wvu.edu Last Updated: 10/14/2011	Services offered: Assays and Measurements Cell Sorting Data Analysis Flow Cytometric Analysis Associations: ISAC COBRE INBRE Cancer Center ABRF NICL
The WVU Flow Cytometry Core Facility provides instrumentation and scientific support for cell analysis and sorting. The facility routinely performs analysis of both eukaryotic and prokaryotic cells for expression of intracellular and extracellular proteins, cell cycle, cell quantitation, cytokine production, and cell sorting based on antigen expression or cell cycle. This facility is currently equipped with two cytometers. The Becton-Dickenson (BD) FACSAria is a 15 parameter (13 fluorescent markers and two scatter parameters) high-speed bench top sorter capable of sorting into 4 different populations. In addition, the FACSAria has a BD aerosol management system for aseptic sorting as well as the ability to maintain temperature during the analysis and sorting of samples. The second instrument in the facility is a BD dual laser FACSCalibur equipped with a sample autoloader and has the capability of 4 color analysis. In addition to the two cytometers, the facility has a Miltenyi Biotec AutoMACS and tissue culture hood for sterile magnetic cell sorting. The Flow Cytometry Core has several software packages including FCS Express, CellQuest Pro, ModfitLT and BD Diva software for data acquisition and analysis. ---- BD FACSCalibur BD FACSAria AutoMac

Integrated Genomics Laboratory Oregon Health and Science University CH14G 3303 SW Bond Ave Portland, OR 97239 (503) 418-2737 harringc@ohsu.edu View Website Primary Contact: Chris Harrington (503) 418-2737 harringc@ohsu.edu Last Updated: 10/14/2011	Services offered: Data Analysis DNA Analysis Genomics Genotyping Microarray Nucleic Acid Extraction Outreach RNA analysis RNA Integrity Sequencing - Next-Gen Sequencing Associations: CTSA Cancer Center ABRF
Microarray services are available through the Gene Microarray Shared Resource for RNA expression profiling, microRNA profiling, DNA genotyping, copy number analysis, and methylation patterns analysis. Currently running Affymetrix and Illumina platforms. Comprehensive RNA and DNA quality assessment services are included. DNA sequencing services are available through the Massively Parallel Sequencing Shared Resource for genome resequencing, transcriptome analysis, miRNA analysis, and promoter analysis via ChIP-seq. ---- The GMSR is actively engaged in protocol optimization for small sample quantities, FFPE samples, and other challenging sample types. The GMSR has optimized protocols for expression profiling using whole blood samples. With standardized operating procedures and a highly experienced staff, the GMSR delivers high quality data to basic science researchers and clinicians. ---- See www.ohsu.edu/gmsr and www.ohsu.edu/mpssr for a listing of major equipment.

Microscopy Imaging Center University of Vermont 203 Health Science Research Facility Burlington, VT 05405 (802) 656-0813 douglas.taatjes@uvm.edu View Website Primary Contact: Doug Taatjes (802) 656-0373 douglas.taatjes@uvm.edu Last Updated: 10/14/2011	Services offered: Assays and Measurements Atomic Force Spectroscopy Cell Imaging Clinical Assessment (Hematology, Clinical Chemistries, etc.) Confocal Microscopy Data Analysis Electron Microscopy Histology Human Pathology Services - Diagnostics & Consultation Immunohistochemistry Laser Capture Microdissection Microscopy Molecular Imaging Optical Imaging Outreach Spectroscopy Support Services - Shared Instrumentation Oversight & Maintenance Confocal Scanning Laser Microscopy Associations: NERTRN EPSCoR Cancer Center CTSA ABRF
At the light and electron microscopic levels, it provides state of the art, quality assured, morphologically oriented services. The MIC is operated on a fee for service basis and provides professional consultation and assistance with equipment use, experimental design and interpretation. The MIC occupies approximately 2000 square feet in the Health Science Research Facility at the University of Vermont and contains the following imaging equipment: 1.JEOL 1400 transmission electron microscope with AMT 11 megapixel digital camera. 2.JEOL JSM 6060 scanning electron microscope with attached Oxford INCA energy dispersive spectroscopy detector for element analysis. 3.Zeiss LSM 510 META confocal scanning laser microscope. 4.BioRad MRC 1024ES confocal scanning laser microscope system. 5.Olympus BX50 research microscope for transmitted light, phase contrast, and epi-fluorescence microscopy. 6. Asylum Research MFP-3D BIO atomic force microscope. 7.CompuCyte Laser Scanning Cytometer. 8.Zeiss Axioscope 2 wide-field fluorescence microscopy system. 9. Arcturus XT-Ti Laser Capture Microdissector system. 10.Olympus IX70 inverted microscope with associated Applied BioPhysics Electri Cell-Substrate Impedance Sensing (ECIS Ztheta) system. 11. A dedicated Dell workstation containing Molecular Devices MetaMorph image analysis software for complex quantitative image analysis. PLEASE SEE OUR WEB SITE FOR DETAILED INSTRUMENT DESCRIPTION AND RECENT PUBLICATIONS. ---- Equipment available: Transmission and scanning electron microscopes, confocal scanning laser microscopes, atomic force microscope, laser capture microdissector, laser scanning cytometer, widefield upright and inverted microscopes,electric cell substrate impedance sensing (ECIS), paraffin microtome, cryostats, ultramicrotomes, and image analysis software.

Stanford Functional Genomics Facility Stanford University 269 Campus Dr CCSR 0120 Stanford, CA 94305-5177 (650) 736-2434 john.coller@stanford.edu View Website Primary Contact: John Coller (650) 736-2434 john.coller@stanford.edu Last Updated: 10/14/2011	Services offered: Assays and Measurements Computational - Bioinformatics Copy Number Variation (CNV) Data Analysis DNA Analysis Genomics Genotyping Library Services Microarray Molecular Library Services Nucleic Acid Extraction Optical Imaging PCR Arrays Phosphor Imaging Proteomics Real-time qPCR RNA analysis RNA Integrity Sequencing - Capillary Sequencing Sequencing - DNA Sequencing Sequencing - Next-Gen Sequencing Support Services - Shared Instrumentation Oversight & Maintenance Western Blot Associations: ABRF Cancer Center WCASRD
The Stanford Functional Genomics Facility (SFGF) provides high-throughput sequencing, microarrays and microarray services to researchers within the Stanford community and beyond. The facility also provides 24/7 access to instruments, equipment and software utilized within the microarray field. We support all arrays based on a microscope format including Agilent, Illumina, Nimblegen, and arrays produced by SFGF. Besides printing cDNA and oligonucliotide microarrays, the facility can also print arrays of your material in a high throughput fashion, including proteins, peptides, antibodies, cell lysates, siRNAs, etc.

Medical University of South Carolina Proteogenomics Facility Medical University of South Carolina 173 Ashley Avenue Charleston, SC 29425 (843) 792-2418 argraves@musc.edu View Website Primary Contact: Scott Argraves (843) 792-5482 argraves@musc.edu Last Updated: 10/14/2011	Services offered: Computational - Bioinformatics Data Analysis Microarray Outreach Real-time qPCR RNA Integrity Surface Plasma Resonance (SPR) Multiplex Bead Array Associations: INBRE EPSCoR COBRE
The MUSC ProteoGenomics Facility offers a range of proteomic and genomics services to the MUSC research community as well as to outside academic and corporate researchers. We perform multiplex bead array analysis (i.e., phosphoprotein, cytokine and miRNA analysis), surface plasmon resonance based biomolecular interaction analysis (i.e., BIAcore), qualitative analysis of RNA (Agilent Lab-on-a-chip Bioanalyzer), DNA microarray screening (i.e., Affymetrix gene expression and SNP analysis), analysis of DNA microarray data and web-based archiving of array data ---- Affymetrix GeneChip Scanner 7G Two Affymetrix GeneChip Fluidics 450 stations Agilent 2100 BioAnalyzer Eppendorf epMotion 5075 Bio-Rad C-1000 Thermal Cycler Bio-Rad iCycle Thermal Cycler Bio-Rad Bio-Plex 200 (Luminex) Biacore 3000

CSIF: Cell Sciences Imaging Facility Stanford University 279 Campus Drive Beckman Center, B050 Stanford, CA 94305 (650) 725-7532 microscopy at stanford.edu View Website Primary Contact: Jon Mulholland (650) 725-7532 jwm at stanford.edu Last Updated: 10/14/2011	Services offered: Cell Imaging Confocal Microscopy Cryo-Electron Microscopy Data Analysis Electron Microscopy Immunohistochemistry Microscopy Molecular Imaging Multiphoton Microscopy Optical Imaging immuno-EM, e-tomography, array tomography Associations: Cancer Center
The Cell Sciences Imaging Facility (CSIF) provides high resolution, state-of-the-art technologies for imaging and analyzing the molecular and structural organization of cells and tissue. The facility offers sophisticated and demanding microscopy techniques (e.g. FRET, FRAP, 2-photon live cell imaging, photo-activation and uncaging, array tomography, immuno-electron microscopy, high pressure freezing...) to Stanford University and industry researchers. The CSIF is organized into three interdependent imaging labs: the Fluorescence Microscopy Core (FMC) which houses multi-photon, confocal and deconvolution microscopes and image analysis software, the Electron Microscopy Core (EMC), which houses high resolution scanning and transmission electron microscopes and the Array Tomography Core (ATC) that provides complete array tomography services. The CSIF, in addition to its Director, Jon Mulholland, is staffed by four research technicians that have expertise and training in electron and light microscopy. ---- MICROSCOPES: 2-photon/confocal microscope (Leica SP5, upright, 2-photon, environmental control, live cell imaging system) Confocal microscope (Leica SP2 AOBS, inverted) Confocal microscope (Zeiss LSM510 with 2-photon laser, environmental control, inverted) Widefield deconvolution microscope (API Delta Vision RT system, stage and objective heater) Automated fluorescence array tomography microscope (Zeiss Axioimager Z1) Transmission electron microscope (Jeol, 1400-TEM) with CCD camera, rt tomography holder, SerialEM automation Transmission electron microscope (Jeol, 1230-TEM) with CCD cameras Field emission scanning electron microscope (Zeiss, Sigma FEG-SEM) Environmental scanning electron microscope (Hitachi, S-3400N VP-SEM) Histology microscopes with color camera (Leica DM1000, DM2000) EQUIPMENT: 4 Ultramicrotomes (Leica Ultracut and cryo ultracut) High pressure freezer w/ rapid specimen exchange (Leica EMPact) Knife maker (includes 1 glass strip) Vacuum (Carbon) evaporator Sputter coater Microwave oven processing Cryo-fixing Equip: Plunge/block freezer Freeze substitution machine Critical Point Dryer (CPD) Imaging/Scanner/printer/CD burner Specimen processing reagent kit Volume rendering, image processing, 64bit, work stations (2x) running Leica and Zeiss software as well as Volocity software

Cryo-Electron Microscopy Facility University of Vermont 120 Health Science Research Facility Burlington, VT 05405 (802) 656-4834 mraderma@uvm.edu View Website Primary Contact: Michael Radermacher Last Updated: 10/14/2011	Services offered: Cryo-Electron Microscopy Electron Microscopy Associations: NERTRN
The research of the cryo-EM laboratories focuses on the three-dimensional structure determination of macromolecular assemblies using electron microscopy combined with image processing. The facility is open to laboratories at UVM who have extensive experience in cryo-electron microscopy. (There are no resources to provide either training or service.)

Northwestern University Skin Disease Research Center Northwestern University 303 E. Chicago Avenue, Ward 9-132 Chicago, IL 60611 (312) 503-4192 sdrc@northwestern.edu View Website Primary Contact: Betsy Cutcher (312) 503-4407 e-cutcher@northwestern.edu Last Updated: 10/14/2011	Services offered: Cell Culture Cell Imaging DNA Analysis Histology Human Pathology Services - Diagnostics & Consultation Immunohistochemistry In Situ Hybridization Laser Capture Microdissection RNA analysis Tissue Culture Viral Vectors Sample Preparation, Specimen Preparation, Specimen Preparation Facility Equipment Associations: MWACD
The goal of the NU-SDRC is to promote first-class translational research in epithelial biology, ultimately to improve patient care. The NU-SDRC is comprised of four distinct cores which include the Administrative Core, the Keratinocyte Core, the Pathology Core and the DNA/RNA Delivery Core. The Core facilities are designed to help NU-SDRC investigators accomplish their research goals related to epithelial cell biology and to provide a level of support to newly recruited junior investigators. ---- Antibody Evaluation, Cell Culture Medium, Cloning of cDNA/RNA, Computer Assisted Image Analysis, Double Labeling, Franz Cell, Frozen Sections, H&E Evaluation, Histochemical Stains, Histology, Human Dermal Fibroblasts, Human Epidermal Keratinocyte, Immortalization of Keratinocytes, Immunohistochemistry, In Situ Hybridization, Laser Capture Microdissection, Mouse Epidermal Keratinocytes, Mouse Phenotyping, Murine Epidermal Keratinocytes, Neonatal Foreskin, Organotypic Raft, Routine Tissue Processing, Sectioning, Skin Disease Tissue, Tissue Acquisition, Tissue Microarray Staining, cloning strategy, expression cassette design, infected cell cultures, keratinocyte cell culture, miRNA, retroviral and lentiviral expression systems, retroviral or lentiviral stocks, shRNA, siRNA, viral expression vectors, viral stock, virus

Protein Chemistry Laboratory Texas A&M University 300 Olsen Blvd TAMU 2128 College Station, TX 77843-2128 (979) 845-2433 jinny@tamu.edu View Website Primary Contact: Jinny Johnson (979) 845-2433 jinny@tamu.edu Last Updated: 10/14/2011	Services offered: 2D-PAGE Assays and Measurements Gel Chromatography Mass Spectrometry SDS-PAGE Sequencing - Protein Sequencing Associations: ABRF
The Protein Chemistry Laboratory performs a wide array of protein analyses including protein/peptide separations and characterization and proteomics analysis. Our major techniques are Amino Acid Analysis, Automated Edman Protein Sequencing, Electrophoresis and Chromatography-based protein/peptide separations and mass spectrometry. In addition, we have expertise and instrumentation for 2D Differential Gel Electrophoresis (2D-DIGE) analysis for high throughput protein expression profiling. We possess Shimadzu/Kratos Axima CFR MALDI-TOF and a Thermo Finnigan LCQ DecaXP Ion Trap mass spectrometers for protein/peptide mass measurement and protein ID from 1D and 2D gels. Our services are available to the entire Texas A&M System as well as outside academic and commercial clients. ---- Amino Acid Analysis available for outside collaborators. 2D DIGE available for outside collaborators. ---- Kratos Axima CFR MALDI-TOF Thermo Finnigan DecaXP ESI-IT Hewlett Packard AminoQuant II GE AKTA Explorer 10 GE IPGPhor DALT6/DALT12 ABI Procise 492 HT

Stem Cell and Xenograft Core (SCXC) University of Pennsylvania 710 BRB-2/3 421 Curie Blvd Philadelphia, PA 19104 (215) 746-0181 gdanet@mail.med.upenn.edu View Website Primary Contact: Gwenn DANET-DESNOYERS Last Updated: 10/14/2011	Services offered: BioBanking Optical Imaging Stem Cell Procurement Surgical Services Xenograft / Xenotransplantation
We provide human adult hematopoietic stem cells, viable leukemia specimens and full service xenotransplanttion services for basic and translational research in the areas of normal hematopoietic and cancer stem cell biology, infectious and metabolic diseases, immunotherapy and regenerative medicine. ---- Viable clinical sample banking

Glass Blowing Shop University of Vermont Chemistry Department Cook Physical Sci Room A213 Burlington, VT 05405-0125 (802) 656-0267 angela.gatesy@uvm.edu Primary Contact: Angela Gatesy angela.gatesy@uvm.edu Last Updated: 10/14/2011	Services offered: Fabrication Support Services - Glassblowing Associations: NERTRN
Fabrication and repair of glassware used for scientific research

High Throughput Analysis Laboratory - HTAL Northwestern University 2205 Tech Drive Hogan Bldg 2-100 Evanston, IL 60208 (847) 491-5960 luanch@northwestern.edu View Website Primary Contact: Chi-Hao Luan (847) 491-5643 luanch@northwestern.edu Last Updated: 10/14/2011	Services offered: Assays and Measurements Biochemical Analysis Cell Culture Cell Imaging Data Analysis Genomics Molecular Imaging Molecular Library Services Nucleic Acid Extraction Outreach Real-time qPCR RNA analysis Thermal Analysis High throughput screening, high content screening, RNAi screening, Analytical, Characterization, Imaging, Other, Programming and Analysis, Real-Time PCR, Sample Preparation, Specimen Preparation, Specimen Preparation Facility Equipment, Spectroscopy, Associations: Cancer Center ABRF MWACD
The HTA provides academic, industrial, and private researchers with equipment and expertise for the development and execution of high throughput biological analysis and screening. The facility is fully equipped with state-of-the-art liquid handling, plate detection and automated microbial culture handling capabilities. ---- ArrayScan VTi high throughout fluorescence imaging system for cellular and high content analysis, Assay development ; enzymatic ; binding ;, FLIPR (96-well and 384-well) for ion channel and GPCR assays, Filter-based and monochromator-based multimode microplate readers (Absorbance, fluorescence, luminescence), High throughput data analysis ; Data automation, Multi-mode plate reader Synergy4 for absorbance , fluorescence ( intensity, anisotropy / polarization ), luminescence with spectral capability. Equipped with two on-board injectors and special filters for AlphaScreen ., Nano-liter liquid handling Nanoliter, QPix II high throughput colony picking and replicatiob system, RNAi library (Human and mouse), Real time PCR RT-PCR Bio-rad iQ5 (96-well) , CFX384 (384-well), Robotic liquid handling workstations (micro-liter and nano-liter), Small molecule chemical compound libraries, TTP Mosquito Crystallization Robot, Fluorescence based Thermal Shift; Thermal Scanning Fluorimetry ; ThermoFluo; Protein ligand screen; Acoustic liquid transfer system Labcyte Echo550

MIT Biopolymers Laboratory Massachusetts Institute of Technology 76-181 attn: Richard Cook Cambridge, MA 02139 (617) 253-1685 cook@mit.edu View Website Primary Contact: Richard Cook (617) 253-1685 cook@mit.edu Last Updated: 10/14/2011	Services offered: Genomics Mass Spectrometry Peptide Synthesis Proteomics RNA analysis Sequencing - Capillary Sequencing Sequencing - DNA Sequencing Sequencing - Next-Gen Sequencing Sequencing - Pyrosequencing Associations: Cancer Center
Fee-or-Service lab serving MIT researchers in: genome sequencing; Sanger DNA sequencing; small scale and multiple peptide synthesis; larger scale peptide synthesis; peptide cellulose array synthesis; MALDI-TOF mass spectrometry and HPLC analytical and preparative scale. ---- Analytical and Preparative scale HPLC ---- Illumina HiSeq2000 Genome Analyzer 3730XL Sanger DNA Sequencers Intavis Multiple Small Scale and Spot Array Peptide Synthesizers Protein Technologies model Tribute Agilent model 1100 HPLC Systems Gilson model 271 Preparative HPLC System Bruker Microflex MALDI

Genomics and Proteomics Core Laboratories University of Pittsburgh 3343 Forbes Avenue Pittsburgh, PA 15260 (412) 648-9440 gpclseq@pitt.edu View Website Primary Contact: Janette Lamb (412) 383-3146 jal18@pitt.edu Last Updated: 10/14/2011	Services offered: 2d Spectra Acquisition and Interpretation 2D-PAGE Assays and Measurements Computational - Bioinformatics Computational - Biostatistics Copy Number Variation (CNV) Data Analysis DNA Analysis Genomics Genotyping Mass Spectrometry Microarray Nucleic Acid Extraction PCR Arrays Proteomics Real-time qPCR RNA analysis RNA Integrity SDS-PAGE Sequencing - Capillary Sequencing Sequencing - DNA Sequencing Sequencing - Next-Gen Sequencing Sequenom genotyping, Methylation analysis Associations: MWACD CTSA ABRF
The Genomics and Proteomics Core Laboratories (GPCL) were founded within the University of Pittsburgh, Office of Research, Health Sciences (OORHS) through an initiative of the Senior Vice Chancellor for the Health Sciences, Dr. Arthur Levine. The laboratories have been developed to foster basic biomedical and clinical research by offering cutting-edge and basic technologies in a cost effective and efficient manner. We offer technical expertise and support with experimental design, protocol development, data analysis and interpretation, as well as assistance with budget and grant preparation. We are always adding new technologies based on the research needs of our clients and are currently equipped with world-class instrumentation and staffed with expert research scientists and technicians. ---- The University of Pittsburgh has outreach programs are primarily directed to clinical and translational researchers and trainees. ---- ABI 3730xl and ABI 3130 DNA analyzer ABI 7900 HT TaqmanTM instrument with blocks for 96 and 384 well microplates as well as for low density arrays and autoloader automation accessory, Affymetrix GeneChip Fluidics station and 3000 Scanner, with autoloader and 7G upgrade Illumina iSCAN system with autoloader2.x, Tecan Freedom evo, Sequenom MassArray Compact System with nanodispenser, Roche GS FLX genome sequencer and AB SOLiD5500xl System. ABI Voyager DE Pro MALDI-TOF-MS, a Bruker MicroTOF LC-TOF MS, a Bruker 12T FTICR MS, a Thermo LTQ Orbitrap Velos with ETD and a TOF-TOF (ABI 4800) instrument with a MALDI ion source and CID. Gateway E-6610D workstation equipped with Core2 Duo T7700 processor, 2GB ECC RAM, and a RAID1 1TB storage, to support running of Roche GS Reference Mapper, Ge De Novo Assembler, and GS Amplicon Variant Analyzer. The GPCL-BAC also hosts a 6 node cluster for Next-Generation sequencing analysis.

Biomolecular Resource Facilities Roswell Park Cancer Institute Elm and Carlton Buffalo, NY 14263 (716) 845-8032 biopolymer@roswellpark.org View Website Primary Contact: Michelle Detwiler (716) 845-8145 michelle.detwiler@roswellpark.org Last Updated: 10/14/2011	Services offered: DNA Analysis Genomics Genotyping Mass Spectrometry Nuclear Magnetic Resonance (NMR) Proteomics Sequencing - Capillary Sequencing Sequencing - DNA Sequencing Associations: WCASRD MWACD ABRF
The Biomolecular Shared Resource provides a suite of services that enable our staff to address a variety of molecularly based research projects. There are three Facilities within the Resource: DNA Sequencing, Proteomics/Mass Spectrometry and Nuclear Magnetic Resonance Spectroscopy (NMR). Our services include Sanger DNA sequencing and fluorescence based DNA fragment analysis; mass spectrometry based proteomics and metabolomics, and NMR spectroscopy. We assist staff in understanding the applications and limitations of various techniques to their research programs through consultation and discussion and development of pilot feasibility studies, as necessary.

Emory Neuroscience NINDS Core Facilities Emory University Emory Center for Neurodegenerative Disease 615 Michael St., 505N Atlanta, GA 30322 (404) 727-4927 nciliax@emory.edu View Website Primary Contact: Nancy Ciliax (404) 727-4927 nciliax@emory.edu Last Updated: 10/14/2011	Services offered: BioBanking Cell Imaging Confocal Microscopy Genomics Genotyping Histology Immunohistochemistry Mass Spectrometry Microarray Microscopy Molecular Construct Services Monoclonal Antibody Proteomics Real-time qPCR RNA analysis Sequencing - DNA Sequencing Sequencing - Protein Sequencing Viral Vectors Virology
The Emory Neuroscience NINDS Core Facilities (ENNCF) is funded by the NIH P30 grant NS055077 and is under the direction of Allan Levey, MD, PhD and Richard Kahn, PhD. It is housed at Emory University in the Center for Neurodegenerative Disease where there is a large neuroscience research community. It includes the following 5 core facilities: Proteomics, Director Nick Seyfried; Microscopy, Director Gary Bassell; Neuropathology/Histochemistry/MAb, Directors Marla Gearing and Chakravarthy Chennareddy; Viral Vector, Director Kerry Ressler; Genetics, Director Michael Rossi. The center has generous institutional support for personnel, space and equipment. ---- Brain bank for neurodegenerative disease Mass spectrometer Zeiss confocal microscope META spectral imaging system Nikon A1 microscope Olympus BX51 upright microscopy for fluorescence, brightfield and DIC Leica DMRBE upright microscope for brightfield and fluorescence Zeiss Axiovert inverted microscope for fluroescence and phase contrast 2 - photon scanning microscope Nikon A1R Live scan confocal microscopy FRAP FRET Illumina Beadstation 500 Beckman Coulter SNPStream ABI TaqMan 7900 ABI 3100 capillary systems Affymetrix GeneChip system KingFisher Flex Magnetic Particle Processor Qigen BioRobot M48 System Tecan Freedom Evo Eppendorf epMotion 5075 Beckman Biomek NX R/Bioconductor GenePattern 3.0 Server Gene Set Enrishcment Analysis Ingenuity Pathway Analysis Spotfire Significancd Analysis of microarrays Gene Cluster 3.0 Java Treeview LTQ Orbitrap Hyrid Mass Spectometer 4000 QTRAP Mass Spectrometer 4700 MALDI-TOF/TOF Mass Spectrometer

Tumor Biology Core Northwestern University Silverman Hall B715 Evanston, IL 60208 (847) 467-5304 i-kandela@northwestern.edu View Website Primary Contact: Andrew Mazar (847) 467-0942 a-mazar@northwestern.edu Last Updated: 10/14/2011	Services offered: Animal Husbandry Cell Culture Cell Imaging Cell Sorting Confocal Microscopy Histology Immunohistochemistry Magnetic Resonance Imaging (MRI) Microscopy Multiphoton Microscopy Necropsy Optical Imaging SDS-PAGE Tissue Culture Western Blot Xenograft / Xenotransplantation Pharmacokinetics, Toxicology Associations: Cancer Center MWACD
The Tumor Biology Core (TBC), under the umbrella of the Center of the Developmental Therapeutic (CDT), provides services focused on supporting the translation of new therapeutics to the clinic. The TBC supports grant applications and can act as an already established translational Core on multi-investigator U and P type grants. In addition to supporting basic research leading to drug translation, the TBC also provides support to clinical investigators interested in advancing new compounds into clinical trials including drug assessment and clinical protocol support. TBC services include a full suite of fee-for-service tumor biology and translational support services including in vitro and in vivo assessment of drug activity and mechanism of action, exploratory drug development activities such as pharmacokinetics and toxicology, consultation and project management and clinical trial support. The TBC staff has extensive expertise in animal disease models, drug development and assessment, and clinical translation available to any Northwestern investigator interested in developing therapeutics or diagnostics. In addition, the TBC will interact with universities and companies in the community that are interested in utilizing the TBC expertise to help advance their translational projects.

Nursing University of Vermont Fletcher Allen Baird 7 Burlington, VT 05405 (802) 847-2793 anthony.minichiello@vtmednet.org View Website Primary Contact: Anthony Minichiello richard.galbraith@uvm.edu Last Updated: 10/14/2011	Services offered: Computational - Bioinformatics Mass Spectrometry Associations: NERTRN
The Research Nurses at the GCRC implement and carry out your project and care for your subjects. The unit, which includes Inpatient rooms, and several multipurpose outpatient procedural rooms, is staffed according to need. The majority of research activity is performed Monday through Friday between the hours of 7:00 am and 3:30 pm. We provide and staff an outpatient area in addition to the inpatient component during these hours. The weekend, evening and night shifts are staffed with one Research Nurse on an as needed basis; with advance notice, additional staffing may sometimes be arranged to accommodate reasonable protocol needs including GCRC research needs in other hospital locations. Services Equipment In addition, there is a Physician's Assistant who is available to assist in conduct of protocols and medical management of research subjects. This service is primarily for those investigators who hold degrees other than the M.D. degree.

COBRE for Perinatal Biology - Mol bio and histology core Women and Infants Hospital of Rhode Island 101 Dudley Street Sunil Shaw, Kilguss -210 Providence, RI 02905 (401) 274-1122 x x8005 sshaw@wihri.org View Website Primary Contact: Sunil Shaw (401) 274-1122 x 8005 sshaw@wihri.org Last Updated: 10/14/2011	Services offered: Cell Imaging Cell Sorting Flow Cytometric Analysis Immunohistochemistry Microscopy Molecular Imaging Optical Imaging Real-time qPCR Support Services - Shared Instrumentation Oversight & Maintenance Associations: NERTRN
We utilize contemporary approaches in cell and molecular biology to address important issues in the development of the mid-to-late gestation fetus. Multi-user facility, open to qualified academic scientists. ---- We offer facilities, training and support to users.

Edward C Carlson Imaging and Image Analysis Core Facility University of North Dakota Anatomy and Cell Biology 501 N Columbia Rd Grand Forks, ND 58202-9037 (701) 777-2579 bryon.grove@med.und.edu View Website Primary Contact: Bryon Grove (701) 777-2579 bryon.grove@med.und.edu Last Updated: 10/14/2011	Services offered: Cell Imaging Confocal Microscopy Electron Microscopy Microscopy Multiphoton Microscopy Optical Imaging Associations: NICL COBRE ABRF
The imaging core provides investigators with access to electron, confocal and fluorescence microscopy equipment. Equipment housed in the facility includes an Hitachi 7500 TEM, an Hitachi 4700 field emission SEM, a Zeiss 510 META confocal microscope system on an Axiovert 200 inverted fluorescence microscope, a Zeiss ConfoCor2 fluorescence correlation spectroscopy (FCS) system , an Olympus FV300 confocal microscope on an Olympus IX70 inverted fluorescence microscope, an Olympus FV1000 MPE multiphoton system for intravital microscopy, a Nikon Eclipse i80 upright fluorescence microscope and a Nikon TE300 inverted fluorescence microscope. Computer workstations and equipment for live cell imaging are also available. Applications using this equipment include TEM, SEM, spectral imaging, multicolor confocal/fluorescence microscopy, FRET, FRAP, FLIP, FCS, ratiometric calcium imaging and standard fluorescence imaging. ---- Hitachi 7500 TEM Hitachi 4700 field emission SEM Zeiss 510 META confocal inverted microscope Zeiss ConfoCor2 FCS system Olympus FV300 confocal inverted microscope(IX70) Olympus FV1000 MPE multiphoton intravital system(BX61) Nikon Eclipse i80 upright fluorescence microscope Nikon TE300 inverted fluorescence microscope

Digital Light Microscopy Core UMass Medical School Biotech II 373 Plantation Street Room 114 Worcester, MA 01605 (508) 856-0045 paul.furcinitti@umassmed.edu View Website Primary Contact: Paul Furcinitti (508) 856-0045 paul.furcinitti@umassmed.edu Last Updated: 10/14/2011	Services offered: Cell Imaging Confocal Microscopy Data Analysis Microscopy Optical Imaging Deconvolution of Images Associations: NERTRN
The Core Digital Light Microscopy Facility provides high-resolution multi-mode light microscopy services to UMass Medical School researchers and investigators at other academic and commercial institutions. Services include wide-field and spinning disk confocal microscopy, fluorescence recovery after photobleaching measurements, live cell imaging, digital deconvolution of microscopy images and image analysis. Training and technical assistance are provided in a user friendly environment. ---- Solarmere CSU10 Spinning Disk Confocal Microscope Olympus IX-70 wide-field digital fluorescence microscope with piezo Z focus drive for digital deconvolution.

Core Optical Imaging Facility University of Texas Health Science Center at San Antonio MC 7762 7703 Floyd Curl Dr. San Antonio, TX 78229 (210) 567-3151 frohlich@uthscsa.edu View Website Primary Contact: Victoria Frohlich (210) 567-3151 lechleiter@uthscsa.edu Last Updated: 10/14/2011	Services offered: 2d Spectra Acquisition and Interpretation Assays and Measurements Cell Imaging Confocal Microscopy Data Analysis Immunohistochemistry Microscopy Multiphoton Microscopy Optical Imaging Optical whole animal imaging Associations: CTSA Cancer Center
Core Optical Imaging Facility is committed to make available state-of-the-art imaging technology to investigators on the UTHSCSA campus and neighboring scientific community. High-end instrumentation for acquisition and analysis of optical data is expensive and requires continued maintenance and improvements. The necessary commitment to this technology is often difficult to maintain within individual laboratories, especially when optical imaging is not a major focus for the laboratory. Therefore, the Core Optical Imaging Facility fills a critical need of UTHSCSA investigators and neighboring scientific community by offering, access to state-of-the-art technology for imaging of living cells, tissues, and animals, consultation, education and assistance regarding the theory and application of optical imaging techniques, technical advice on specimen preparation techniques and probe selection. ---- FRET, FLIM, intravital imaging

Core Biology Facility North Dakota State University Dept of Chemistry and Biochemistry, IACC 334, 1320 Albrecht Blvd Dept. 2710, PO Box 6050 Fargo, ND 58108-6050 (701) 231-5334 t.wang@ndsu.edu View Website Primary Contact: Tao Wang (701) 231-5334 t.wang@ndsu.edu Last Updated: 10/14/2011	Services offered: Assays and Measurements Biochemical Analysis Cell Culture Cell Imaging Cell Sorting Data Analysis DNA Analysis Flow Cytometric Analysis Gel Chromatography Genotyping Microarray Microscopy Nucleic Acid Extraction PCR Arrays Phosphor Imaging Real-time qPCR RNA analysis RNA Integrity SDS-PAGE Spectroscopy Support Services - Shared Instrumentation Oversight & Maintenance Tissue Culture Western Blot Associations: EPSCoR COBRE ABRF INBRE
The Center for Protease Research Core Biology Facility (CBF) was established to provide access to equipment and specialized training in the areas of molecular and cell biology and tissue culture in order to enhance the biomedical research environment at NDSU and around the area. We provide student training and consultation on experimental design and data interpretation. The facility also run experiments for clients. ---- Storm 865 Imager Nanodrop 2000C Spectrophotometer Veriti 96-well ABI thermal cycler Agilent 2100 Bioanalyzer Two ABI 7500 Fast Real-Time PCR Machines 2 ABI 2720 Thermal Cyclers Axon 4000B Genepix Microarray Scanner Allegra 25R table-top centrifuge Eppendorf 5417R refridgerated microcentrifuge Eppendorf 5430 microcentrifuge Beckman Coulter DU 530 UV/Vis Spectrophotometer with Microcell-cuvette Accuri C6 Flow Cytometer Gemini EM Fluorimeter Multiskan Spectrum microplate spectrophotometer

Transgenic and Genetic Constructs Shared Resource Dartmouth Medical School 1 Medical Center drive Lebanon, NH 03756 (603) 653-9966 steven.fiering@dartmouth.edu View Website Primary Contact: Steve Fiering (603) 653-9966 steven.fiering@dartmouth.edu Last Updated: 10/14/2011	Services offered: Animal Husbandry Cell Culture Embryo Cryopreservation & Recovery Services Genotyping Necropsy Surgical Services Tissue Culture Transgenics Associations: INBRE COBRE Cancer Center
The Transgenic core supports the generation and utilization of genetically modified mice. We design and produce genetic constructs with the following methods: yeast and E.coli 'recombineering', ES cell targeting (transfection, drug selection, and expansion). We produce transgenic mice: DNA injections into oocytes, ES cell injections into blastocysts. We also provide two humanized mouse models. Cryopreservation services are also available. There are many technician services (animal husbandry, IP and IV injections, blood drawing, necropsy and surgeries- embryo transfers, ovariectomy, vasectomy

COCVD Obesity Research Core University of Kentucky, Lexington Room 521B, Charles T. Wethington Building 900 S. Limestone Lexington, KY 40536 (859) 218-1419 wkatz@uky.edu View Website Primary Contact: Wendy S. Katz, Ph.D. (859) 218-1419 wkatz@uky.edu Last Updated: 10/14/2011	Services offered: Assays and Measurements Associations: COBRE
The primary focus of the COCVD is to idenfiby mechanisms linking obesity to cardiovascular disease. The Obesity Core provides the equipment and technical expertise for measurement of lean/fat mass and whole body metabolism in mice. The DEXA-R and/or Echo MRI are utilized for non-invasive quantitative assessment of lean and fat mass and bone mineral density in mice. The TSE LabMaster system for indirect calorimetry carries out computerized quantitative measurement of oxygen consumption food intake, water intake, and physical activity in mice. This system is localized to dedicated space within the Division of Laboratory Animal Resources and is available to investigators of the COCVD. The TSE PhenoMaster system quantifies food and water intake, and measures and collects urine and feces for analysis. ---- Echo MRI Dexa-R TSE LabMaster 24-chamber system for indirect calorimetry TSE PhenoMaster 8-chamber system for metabolism study.

Stowers Molecular Biology Facility Stowers Institute for Medical Research 1000 East 50th Street Kansas City, MO 64110 (816) 926-4416 molbio@stowers.org View Website Primary Contact: Karen Staehling (816) 926-4416 ksh@stowers.org Last Updated: 10/14/2011	Services offered: Copy Number Variation (CNV) DNA Analysis Genomics Genotyping Microarray Nucleic Acid Extraction PCR Arrays Real-time qPCR RNA analysis Sequencing - Capillary Sequencing Sequencing - DNA Sequencing Sequencing - Next-Gen Sequencing automation, mutagenesis, recombineering Associations: MWACD ABRF
The Molecular Biology Facility supports investigators in their research endeavors by providing high quality services, collaborative project potential and access to state of the art technology. Current routine services include DNA sequencing, site-directed mutagenesis, recombination-mediated genetic engineering, plasmid preparations and distributing clones/vectors from our in house collections. We also support real time quantitative PCR through instrument training, troubleshooting and experimental design. Stowers researchers also have access to the latest next-generation sequencing and microarray technologies within our facility. We have an Illumina Genome Analyzer IIx platform and can assist with high-throughput genome-sequencing, RNA-seq and ChIP-seq projects. We currently support commercially available Affymetrix and Agilent microarray platforms. Common microarray experiments include ChIP- chip, array CGH, and expression applications. We utilize liquid handling and colony manipulation robots to automate many of our services and improve quality. In addition, we provide automation expertise and collaborate with researchers on custom automation projects. Examples of collaborative automation projects include large-scale yeast synthetic lethal screens and generation of a histone alanine-scanning library. ---- * ABI 3730 DNA Analyzers (2) * Biomek® FX Liquid Handling Robots (2) * Tecan Freedom EVO Liquid Handling Robot * Qpix2 Colony Picking Robot * Singer RoTor HDA robot * Hydra II Microdispenser * CAS 4200 PCR Setup Robot * QIAgility PCR Setup Robot * ABI 7500 Real Time System * ABI 7900HT Real Time System * Maxwell 16 Instrument for nucleic acid and protein purification * Caliper Labchip 90 System * Agilent BioAnalyzer * Affymetrix GeneChip® Scanner 3000 * Affymetrix GeneChip Fluidics Station 450 * Agilent Array Scanner * Illumina Genome Analyzer IIx and Cluster Station * Spectramax M2e Microplate reader * Matrical Bioscience SonicMan Plate Sonicator * MJ Research Tetrad® Thermocyclers (4)

COCVD Pathology Core University of Kentucky, Lexington Room 577, Charles T. Wethington Building 900 S. Limestone Lexington, KY 40536 (859) 218-1419 wkatz@uky.edu View Website Primary Contact: Wendy S. Katz, Ph.D. (859) 858-1419 wkatz@uky.edu Last Updated: 10/14/2011	Services offered: Histology Immunohistochemistry Microscopy Associations: COBRE
The Pathology Research Core facilitates the COCVD projects by providing equipment and technical expertise to embed and section tissue specimens and perform chemical and immuno-histological staining of tissue sections to be examined, photographed and analyzed chromogenically by light microscopy. ---- Shandon Citadel 12-step programmable tissue processor Leica EG1160 Paraffin embedding center Shandon Finesse 325 Microtome Nikon 55i Cool LED Upright Microscope NIS-Elements-BR Quantitative Analysis Software Package

Penn Molecular Profiling Facility University of Pennsylvania 277 John Morgan 3620 Hamilton Walk Philadelphia, PA 19104 (215) 898-3675 dbaldwin@mail.med.upenn.edu View Website Primary Contact: Don Baldwin (215) 898-3675 dbaldwin@upenn.edu Last Updated: 10/14/2011	Services offered: Computational - Bioinformatics Copy Number Variation (CNV) DNA Analysis Genomics Genotyping Microarray Nucleic Acid Extraction PCR Arrays Real-time qPCR RNA analysis RNA Integrity Sequencing - Next-Gen Sequencing Associations: ABRF Cancer Center
Genomics services for RNA and miRNA profiling, SNP genotyping, genomic tiling applications, deep sequencing. Data management and analysis services. ---- Affymetrix, Illumina, Sequenom, Fluidigm, Luminex, TaqMan, Chemagen, BioMek

Research Department Shriners Hospital for Children 3101 SW Sam Jackson Park Road Portland, OR 97239 (503) 221-3445 kdz@shcc.org View Website Primary Contact: Keith Zientek (503) 928-9224 kdz@shcc.org Last Updated: 10/14/2011	Services offered: Biochemical Analysis Computational - Bioinformatics Confocal Microscopy Electron Microscopy Mass Spectrometry Peptide Synthesis SDS-PAGE Sequencing - Capillary Sequencing Sequencing - Protein Sequencing Support Services - Shared Instrumentation Oversight & Maintenance Associations: ABRF
Shared resource core providing peptide synthesis/sequencing (N-terminal and AAA), oligo sequencing, and mass spectrometry proteomics resources.

Biopolymers Facility Harvard Medical School NRB 088 77 Avenue Louis Pasteur Boston, MA 02115 (617) 432-7480 director@genome.med.harvard.edu View Website Primary Contact: Robert Steen (617) 432-7480 director@genome.med.harvard.edu Last Updated: 10/14/2011	Services offered: Copy Number Variation (CNV) DNA Analysis Genomics Genotyping Library Services Nucleic Acid Extraction oligo synthesis PCR Arrays Real-time qPCR RNA analysis RNA Integrity Sequencing - Capillary Sequencing Sequencing - DNA Sequencing Sequencing - Next-Gen Sequencing Support Services - Central Laboratory Supply Support Services - Shared Instrumentation Oversight & Maintenance Associations: ABRF
-About the BPF- The Biopolymers Facility is a non-profit technology service center at Harvard Medical School. The investigators at Harvard Medical School make up the primary client base of the BPF. We also serve labs at schools, universities and biotechnology companies from all across the country. Services offered include: -DNA Sequencing (AB 3730xl) -Next-Generation DNA Sequencing (Illumina HiSeq2000) -Next-Generation Library Sample Prep -Genotyping, GeneExpression and microRNA profiling (AB 7900HT) -Bioanalyzer Analysis DNA and RNA (Agilent 2100 BioAnalyzer and 2200 TapeStation) -DNA Prep (in plates) -Qiacube Automated DNA / RNA Prep (single to multiple samples) -Luminex LS200 XMap Assays -Custom Automation Services on Hamilton StarPlus Robotic Liquid Handler -Oligonucleotide Ordering (IDT Portal) -Reagents and Supplies Ordering (multiple vendors / products) The facility has a comprehensive web based Laboratory Information Management System (LIMS) which provides our users with access to order services and supplies as well as retrieve data and review and pay invoices all online. ---- Advalytix / Beckman Coulter AmpliSpeed Slide Cycler Agilent 2100 Bioanalyzer Agilent 2200 TapeStation Applied Biosystems 7900HT Applied Biosystems 3730xl Axon GenePix 4000B Covaris S2 Hamilton StarPlus Robotic Liquid Handler Illumina HiSeq2000 (2 Instruments) Illumina cBot Cluster Station (2 Instruments) Luminex LS200 MJ Tetrad DNA Engines (5 Instruments 384 and 96 well blocks) NanoDrop ND8000 Qiagen QiaCube Robot Tecan Genios

Marine DNA Sequencing and Analysis Center Mount Desert Island Biological Laboratory PO Box 35 Old Bar Harbor Rd. Salisbury Cove, ME 04672 (207) 288-9880 x 130 mdibl_info@mdibl.org View Website Primary Contact: Christine Smith (207) 288-9880 x 130 cmsmith@mdibl.org Last Updated: 10/14/2011	Services offered: Computational - Bioinformatics Data Analysis DNA Analysis Genomics Genotyping Microarray Nucleic Acid Extraction oligo synthesis Real-time qPCR RNA analysis RNA Integrity Sequencing - Capillary Sequencing Sequencing - DNA Sequencing Associations: INBRE
The Mount Desert Island Biological Laboratory is an independent, not-for-profit marine and biomedical research facility and an international center for comparative physiology, toxicology and regenerative biology studies. ---- MDIBL provides traditional capillary sequencing services to internal and external users. Expressed sequence tags (ESTs) are produced for six marine species from the Gulf of Maine and are available in GenBank and library clones are available upon request. Oligonucleotide microarrays based on ESTs and other transcripts are available for killifish, lobster, green crab, and copepod.

Genomics and Proteomics Core Facility Brown University 70 Ship St Providence, RI 02912 (401) 863-2875 Christoph_Schorl@brown.edu View Website Primary Contact: Christoph Schorl (401) 863-2875 Christoph_Schorl@brown.edu Last Updated: 10/14/2011	Services offered: Data Analysis Flow Cytometric Analysis Genomics Microarray Real-time qPCR RNA analysis RNA Integrity Sequencing - Capillary Sequencing Sequencing - DNA Sequencing Sequencing - Next-Gen Sequencing Western Blot Associations: NERTRN COBRE
The Genomics and Proteomics Core Facility at Brown University is part of the COBRE Center for Cancer Signaling Network and its facilities are located at the Laboratories for Molecular Medicine at 70 Ship Street in Providence, RI. The facility offers state of the art equipment in genomic and proteomic research, as well as technical expertise, support and consultation on experimental design in these areas to all interested investigators through the director of the Core Facility. The Genomics Core Facility recently introduced Next-Generation Sequencing Service using Illumina technology. Please feel free to contact Christoph Schorl (Christoph_Schorl@brown.edu) with any questions and/or suggestions at 401-863 2875 or by email. I am looking forward to collaborating on exciting scientific projects with many of you in the future. ---- Ultra centrifugation, qPCR configuration for high throughput and TLDA arrays

Protein and Nucleic Acid Facility (PAN) Stanford University 279 Campus Drive West Beckman Center, Room B065 or B017 Stanford, CA 94305 (650) 723-1907 meckart@stanford.edu View Website Primary Contact: Michael Eckart (650) 723-1907 meckart@stanford.edu Last Updated: 10/14/2011	Services offered: Copy Number Variation (CNV) DNA Analysis Genomics Genotyping Mass Spectrometry Microarray oligo synthesis Peptide Synthesis Real-time qPCR RNA analysis RNA Integrity Sequencing - Capillary Sequencing Sequencing - DNA Sequencing Sequencing - Protein Sequencing Sequencing - Pyrosequencing Surface Plasma Resonance (SPR) Surface Plasmon Resonance (SPR; Biacore) Associations: Cancer Center ABRF WCASRD
The Protein and Nucleic Acid (PAN) facility is a multifaceted biotechnology fee-for-service laboratory combining technology with expertise in genomics and protein analytics to support biological and biomedical research by providing a diverse array of services to not only the Stanford research community but also other non-profits and biopharmaceutical organizations. The services provided include: • Gene Expression Analysis • Microarrays (Full service) • Real-time PCR (Full service or investigator use) • Pyrosequencing using PyroMark technology for methylation analysis and sequence variation analysis(Full service or investigator use) • Biomolecular Interaction Analysis (Surface Plasmon Resonance; Biacore) • DNA Sequencing • Oligonucleotide Synthesis • Protein Sequencing • Peptide Synthesis • Protein Identification • Mass Spectrometry Each of our services is staffed and maintained by highly experienced and dedicated scientists. We can provide as much assistance as needed, from the initial study design through all the procedures needed for an experiment to the final interpretation and analysis of data. Our services include standard assays, as well as customized services. We are always interested in developing new assays, or adapting existing established assays to address a specific research question.

Mass Spectrometry for Small Molecules University of North Dakota 501 N Columbia Rd University of North Dakota, School of Medicine, Room 3570 Grand Forks, ND 58202 (701) 777-2305 mikhail.golovko@med.und.edu View Website Primary Contact: Dr. Golovko (701) 777-2305 mikhail.golovko@med.und.edu Last Updated: 10/14/2011	Services offered: Assays and Measurements Data Analysis Mass Spectrometry Associations: COBRE
Quantitative Analysis of Small Molecules by UPLC or HPLC Coupled with Tandem Mass Spectrometry Bioactive lipids -Prostaglandin profiling (COX products) -Products of lipid peroxidation (isoprostanes) -Hydroxy-Fatty Acids profiling including resolvins (LOX and P450 products) Hydroxycholesterol quantification Carbohydrates Nucleotides and nucleosides (adenosine, DNA modifications) Lipid profiling HNE (products of fatty acid peroxidation) Qualitative Analysis of Small Molecules by Direct Infusion or UPLC Analysis Coupled with Tandem Mass Spectrometry Structure and elemental composition prediction using high resolution accurate mass analysis Metabolomics (comparison of metabolites between different experimental groups e.g. wild-type and genetically modified, control and treated) Drug Metabolism (determination of molecule biotransformations) Qualitative and Quantitative Analysis of Small Volatile Molecules Using GC/MS with Various Ionizations techniques, e.g., EI, NICI, CI. Fatty acid analysis ---- Synapt G2 HD Q-TOF Mass Spectrometer with Ion Mobility Technology with UPLC Waters Aquity system (Waters) High resolution (40,000) accurate mass (2 ppm) instrument with ESI, APCI, APPI, Solid Probe ion sources, ion mobility cell, and TriVersa NanoMate Allows for MS and MSe analysis, exact mass, elemental composition, and structure determination of unknown compounds Metabolomics profiling and experimental group metabolome comparision API 3000 Triple Quadrupole Mass Spectrometer (Applied Biosystems-Sciex) coupled to Agilent 1100 HPLC High sensitivity, low-resolution instrument with ESI and APCI ion sources Allows for a targeted quantification of known compounds using LC-MS/MS approach Available for self operation after training. Polaris Q Trap Mass Spectrometer coupled to TRACE GC Ultra (ThermoFinigan) Low-Resolution, EI and CI, GC-MS and GC-MS/MSn Allows for quantification of volatile compounds, often requires derivatization Available for self operation after training.

Proteomics Core Wayne State University 540 E. Canfield, Scott Hall, R. 2105 Detroit, MI 48201 (313) 577-6545 pmstemmer@wayne.edu Primary Contact: Paul Stemmer (313) 577-6536 pmstemmer@wayne.edu Last Updated: 10/14/2011	Services offered: Data Analysis Mass Spectrometry Proteomics SDS-PAGE Western Blot Associations: ABRF
Protein identification and proteome analysis using LC-MS/MS platform. Affinity selection for phosphopeptides with analysis using CID and ETD. Validation and quantitation of proteins using MRM with a TSQ Vantage triplequad. ---- LTQ-XL with ETD ion trap LC-MS/MS TSQ Vantage tiplequad LC-MS/MS

Optical Imaging Immune Disease Institute 200 Longwood Ave Boston, MA 02115 (617) 713-8299 leung@idi.harvard.edu View Website Primary Contact: Harry Leung (617) 713-8299 leung@idi.harvard.edu Last Updated: 10/14/2011	Services offered: Cell Imaging Confocal Microscopy Microscopy Multiphoton Microscopy Optical Imaging in vivo/in vitro imaging
confocal/multiphoton microscopy, image analysis ---- Olympus Fluoview FV1000 confocal system Ziess/BioRad Radiance 2100MP multiphoton system Marianas confocal, with Yokogawa CSU22 Spinning Disk

Bioimaging Core Facility University of Nebraska Medical Center Department of Radiology 981045 Nebraska Medical Center Omaha, NE 68198-1045 (402) 559-3138 mboska@unmc.edu View Website Primary Contact: Michael Boska (402) 559-3138 mboska@unmc.edu Last Updated: 10/14/2011	Services offered: 2d Spectra Acquisition and Interpretation Clinical Imaging - Small Animal (X-ray, Ultrasound, microCAT, MRI) Computational - Application Development Data Analysis Functional Brain Imaging Magnetic Resonance Imaging (MRI) Spectroscopy Associations: COBRE
The facility includes two 7 Tesla horizontal-bore MRI/MRS systems, a GE Healthcare Gamma Medica-Ideas (GEM) Triumph multimodal platform equipped with SPECT and CT scanners, various physiological monitoring capabilities including ECG monitoring/gating, respiratory monitoring/gating, as well as temperature monitoring and control throughout the imaging experiment. The facility also includes an array of Solaris, Linux, and Windows based computers with custom and specialized software for image and spectroscopic image processing. The facility will provide guidance for post-processing of image data and experimental planning for longitudinal studies. ---- The Bruker (Karlshure, Germany) Biospec 7T/21cm MRI/MRS system with a full range of options is installed in our laboratory. The system operates on a Linux workstation and includes a full range of imaging and spectroscopy pulse sequences and associated analysis programs. The 2-channel RF system includes a 400W pulse power RF amplifier for the proton channel (300 MHz) and a broadband 1 Kw X-nucleus channel. The system includes actively decoupled proton transmit coil (7 cm ID) and a reception surface coils (two) optimized for rat and mouse brain studies, respectively. Also included are: (1) double tuned 3 cm surface coil for 1H/31P or 1H/13C investigations; (2) a high performance gradient insert for NMR microscopy studies; (3) three proton RF coils for imaging studies using the high performance gradient insert (15 mm, 25 mm and 35 mm ID); (4) an eight-channel physiological monitoring system (Bruker) integrated with the scanner to allow for gating and motion correction of images, which includes optically coupled EKG and respiratory monitors; (5) Unix workstations for offline data processing (See Computers for details); and (6) we have developed a suite of laboratory built animal holders and coils. The Bruker Pharmascan system (7T/16 cm) includes a two channel actively decoupled volume transmit coil/surface coil system (Insight Neuroimaging Systems, Worcester MA), physiological monitoring system, and upgraded gradients and shim and actively shielded gradient system (BGA9 system, Bruker, gradient strength 360 mT/m, equipped with z0 , z2 , zx, zy, x2- y2, xy shims) and the Bruker spectroscopy and diffusion tensor imaging (DTI) packages. This will allow pertinent developments in mouse brain spectroscopic imaging and DTI to be directly applied to the new scanner. One preclinical single photon emission computed tomography (SPECT) and computed tomography (CT) dual scanner (GammaMedica-Ideas (GM-I) Flex Triumph Multimodal Preclinical Platform) equipped with multipinhole collimators, variable radius of rotation heads (1.5 cm to 17.5 cm) and four interchangeable patient beds to accommodate animals from mice to small primates. GM-I’s new VIVID (Volumetric Image Visualization, Identification and Display) software package has been developed specifically for FLEX Triumph with a streamlined user interface for automated fusion and display, automated loading of SPECT, PET and CT image data, and an automated visualization module for 2D, 3D, and single or multiple (fused) images. Equipment Quantity: 1. Unix workstations and PCs for offline data analysis - 10 2. Linux servers – 4 3. Sun Fire x4500 servers – 3 4. Sun Blade 2500 systems – 4 5. Sun Fire V240 servers with fast access disk array (20 TB) – 2 6. Surface coils - 8 7. Volume coils - 6 8. Anesthesia machines - 6 9. Small animal ventilator - 1 10. Animal physiology monitors - 4 11. Laptop and desktop computer systems - 20

Bio-NMR Laboratory University of Kansas, Lawrence Del Shankel Structural Biology Center 2034 Becker Drive Lawrence, KS 66047 (785) 864-3746 asokan@ku.edu View Website Primary Contact: Dr. Asokan Anbanandam (785) 864-3746 asokan@ku.edu Last Updated: 10/14/2011	Services offered: 2d Spectra Acquisition and Interpretation Data Analysis Nuclear Magnetic Resonance (NMR) Spectroscopy Protein structure and dynamics in solution Associations: COBRE
The COBRE Biomolecular NMR Laboratory maintains two high field NMR spectrometers in support of structural and dynamics studies of biomolecules with COBRE and other investigators in Kansas and the region. Its capabilities include determining high resolution structures, biological macromolecules, elucidation and structural mapping of protein-protein, protein-nucleic acid, protein-peptide, protein-drug interactions, and studies of the dynamics of proteins and their complexes in solution. Laboratory staff provide advice, consultation, training, assistance and complete structure elucidation services to investigators. The staff is also responsible for maintenance, upgrades, implementation of new NMR pulse sequences, and assisting local and remote users with technical problems. ---- Bruker Avance 800 MHz NMR; Bruker Avance III 600 MHz NMR Close access to protein production facilities for isotopic labeling of proteins.

Nemours - AI duPont Hospital for Children Nemours 1600 Rockland Rd Department of Biomedical Research Wilmington, DE 19803 (1) 302-651 x 6827 cbarone@nemours.org View Website Primary Contact: Carol Barone (1) 302-651 x 6827 cbarone@nemours.org Last Updated: 10/14/2011	Services offered: BioBanking F.I.S.H. Histology Human Pathology Services - Diagnostics & Consultation Immunohistochemistry In Situ Hybridization Laser Capture Microdissection Microscopy Necropsy Outreach Protocol Development/Clinical Trial Coordination Support Services - Biological photography/Photomicrography Support Services - Shared Instrumentation Oversight & Maintenance Ab development Associations: INBRE COBRE Cancer Center ABRF NICL
The Histotechnology Core Facility is part of the Department of Biomedical Research, at Nemours and operates independently from the hospital anatomical pathology lab. The Core is CLIA certified #08D0878042 and provides clinical diagnostic testing for muscle enzyme histochemistry and IHC, as well as routine and complex histology for research. The Core serves internal academic, external academic and corporate clients for the following: routine histology, specialy stanining, enzyme histochemistry, immuno histochemistry (IF and IHC) in-situ, LMD, antibody development, imaging and morphometrics (histograms). ---- Nemours Research: has many other core services and laboratories available to both physicians, principle investigators, and corporate clients. The core is CLIA certified. COBRE/INBRE and other grants. Associate higher education faculty. ---- Light and Fluorescent microscopes, microtomes, cryostats, tissue processor, embedding station, automated H&E staining, automated and manual immuno staining, imaging software(ImagePro Plus 6.0), Muscle Histograms, Laser Microdissector, Micro Probe Centers, Tissue PCR (Perkin- Elmer) and 2D Barcoding for blind studies.

Quantitative Bioelemental Imaging Center - QBIC Northwestern University 2170 Campus Drive, Room B540 Silverman Hall Evanston, IL 60208 (847) 467-7282 r-copeland@u.northwestern.edu View Website Primary Contact: Rebecca Marvin (847) 467-7282 r-copeland@u.northwestern.edu Last Updated: 10/14/2011	Services offered: Cell Imaging Confocal Microscopy Mass Spectrometry Microscopy Molecular Imaging Multiphoton Microscopy Optical Imaging Spectroscopy Metal Analysis Associations: MWACD
Metal Analysis: • ThermoFisher X Series II ICP-MS: The X Series II is a quadrupole based Mass Spec for the detection of most elements in the low ppb concentration range (usually 0.2-100 ppb). The ICP-MS is equipped with Collision Cell Technology (CCT) to reduce interferences from doublets for accurate detection of V, Cr, Fe, As, and Se. It is also equipped with an organics kit to expand sampling capabilities for a variety of matrices. QBIC has recently developed a new technique for analyzing samples of 50uL and below with less than part-per-billion detection limits. • PerkinElmber Atomic Absorption Spectrometer (AA): The AA is equipped with a graphite furnace and flame atomizer for both low and high volume samples, respectively. The graphite furnace can accommodate down to 10uL injections for ultra small sample sizes. • New Wave UP-213 Laser Ablation (LA) system: The laser ablation system is interfaced to the ICP-MS for metal detection of ablated solid materials. The LA system uses a deep UV laser to ablate a wide range of materials, from tissue specimens to geological samples. • ThermoFisher SpectraSystem HPLC: The HPLC is designed to interface with the ICP-MS for sample speciation and metal detection. Analytical protocols for the separation of As(III) and As(V) have been developed; however, further method development may be required for specific customer needs. Imaging: • Zeiss Axio Observer.Z1 Inverted Confocal Microscope: The inverted confocal microscope is equipped with an Argon laser (458, 477, 488, and 514nm), two HeNe lasers (543 and 633nm) and a 2-photon laser (described below). The Axio Observer.Z1 is completely digital providing added sensitivity and performance for exceptional quality images. • Zeiss LSM 510 Upright Confocal Microscope: The upright confocal microscope is also equipped with an Argon laser, two HeNe lasers, and a 2-photon laser. By design, the upright confocal provides an ideal platform with dipping objectives for imaging of solvent immersed samples. The microscope is also equipped with a tunable Meta detector with multi-wavelength detection capabilities. • Spectra Physics Mai Tai 2-photon Laser: Both fluorescence microscopes are interfaced with the digitally controlled 2-photon laser. This tunable Near IR pulsed laser is capable of providing excitation over the full UV and visible range for simultaneous excitation of multiple fluorophores. 2-photon imaging is ideal for live, deep-tissue microscopy by providing a low energy excitation for reduced phototoxicity. Contact Rebecca Marvin for more information at r-copeland@u.northwestern.edu ---- Search list: 2-photon Two-photon two 2 photon laser Spectra Physics Mai Tai titanium sapphire, Confocal Fluorescence Microscopy Zeiss LSM 510 Axio Observer.Z1 Inverted Upright, HPLC ICP-MS HPLC-ICP-MS high performance liquid chromatography inductively coupled plasma mass spectrometry ICP, Inductively Coupled Plasma Mass Spectrometry ICP-MS ICP ICPMS X Series II ThermoFisher, Laser Ablation inductively coupled plasma mass spectrometry LA ICP-MS LA-ICP-MS ICP, Meta detector confocal microscopy multiple wavelength detection

Jerome B. Cohen X-ray Diffraction Facility Northwestern University 2220 Campus Drive Cook Hall, 1016 Evanston, IL 60208 (847) 491-7810 j-carsello@northwestern.edu View Website Primary Contact: Jerry Carsello (847) 491-7810 j-carsello@northwestern.edu Last Updated: 10/14/2011	Services offered: Single Crystal X-ray Pattern Analysis X-Ray Crystallography X-ray Diffraction and Scattering Analytical, Characterization, Computer Hardware/Software Associations: MWACD
The primary function is to provide general-purpose x-ray equipment for diffraction and fluorescence studies. The facility can also make accommodations for non-routine experiments such as, special attachments for high and low temperatures, vacuum or protective atmospheres, monochromators, special detectors, etc. ---- Facility Description Function: The primary function is to provide general-purpose x-ray equipment for diffraction and fluorescence studies. The facility can also make accommodations for non-routine experiments such as, special attachments for high and low temperatures, vacuum or protective atmospheres, monochromators, special detectors, etc. Examples of current measurements are: powder diffraction, single-crystal diffraction, thin-film reflectivity, thin-film diffraction, crystal truncation rod scattering, small angle scattering, Laue diffraction, wave-length dispersive x-ray fluorescence, x-ray standing waves and high-resolution x-ray diffraction. Equipment: There are presently thirteen experimental x-ray stations available, four of which have rotating anode sources. Computers and Software: All of the x-ray stations operate via networked PC's with software that allows for control via stepping motors and data collection via counters. A networked printer is available. ICDD database, JADE diffraction analysis, CaRine crystal builder simulation and Laue diffraction software packages are available. SPEC and CPLOT are available on four of the stations. Available apparatus * Rigaku ATX-G Thin-film Diffraction Workstation: A high intensity 18kW copper x-ray source is coupled to a multilayer mirror. The system has selectable x-ray optical configurations suitable for work with single crystal, thin-film or poly-crystalline film samples. Also supported are grazing incidence and in-plane diffraction geometries. Other features are the 5-axis goniometer with several 4-crystal monochrometers that couple to the multilayer mirror. * Rigaku S-MAX 3000 High Brilliance SAXS System Rotating anode with multilayer mirror, Bruker Hi-Star 2-D wire detector, SAXS-GISAXS, WAXS capabilities including high temperature. q(min) = 0.04 Å-1 * 18kW Rigaku: High-resolution 4-circle diffractometer with Osmic Max-Flux multi-layer mirror, coupled to Si or Ge monochromator, SPEC software control, high-count rate scintillation detector, automated beam attenuator and multi-channel analyzer fluorescence spectroscopy system. * 18kW Rigaku: Medium resolution 4-circle diffractometer with SPEC software control and automated beam attenuator. * 12kW Rigaku: 2-circle diffractometer with Osmic Max-Flux multi-layer mirror, high-count rate scintillation detector and multi-channel analyzer fluorescence system. * Scintag XDS2000: Automated diffraction system, with four-circle pole-figure and residual stress device, thin film diffraction attachment and solid-state detector. * Rigaku Dmax: Automated powder diffraction stations featuring Jade Analysis software. * PC data analysis workstation: JADE 9.0 powder diffraction analysis software, CaRine diffraction simulation software, ICDD powder diffraction database and Laue analysis with digital scanner and Orient Express analysis software. * Blake High Resolution tangential goniometer: 5-Bounce high-resolution rocking curve-scan system. Sealed tube x-ray source. Other available equipment: * A variety of x-ray anode targets and source sizes to accommodate the four high intensity rotating anode generators and the eight sealed- tube x-ray generators. * Various Si, Ge, Graphite and LiF crystals and multi-layer mirrors are available for incident or diffracted beam monochromators. * Environmental chambers for vacuum, atmosphere, low temperature (>2 K) or high temperature (<2300 K) operation. * Solid-state detectors including a Vortex high count rate detector with associated electronics, SCA’s and MCA's. * Three gas-filled 1-dimesion linear position sensitive detectors. * Variety of film cameras: Debye-Scherrer, Laue, rotating crystal, cylindrical, topographic, Buerger precession, and darkroom facilities

Cell Analysis Facility (Flow Cytometry) University of Nebraska Medical Center 68198 Nebraska Medical Center Omaha, NE 68198-5816 (402) 559-6267 ckuszyns@unmc.edu View Website Primary Contact: Dr. Charles Kuszynski (402) 559-6299 ckuszyns@unmc.edu Last Updated: 10/14/2011	Services offered: Assays and Measurements Biochemical Analysis Cell Sorting Data Analysis DNA Analysis Flow Cytometric Analysis Protocol Development/Clinical Trial Coordination Support Services - Shared Instrumentation Oversight & Maintenance Multiplex assays Associations: EPSCoR COBRE Cancer Center ABRF INBRE MWACD
The UNMC Cell Analysis Facility provides flow cytometry services to investigators on and off the UNMC campus on a fee for service basis. Services include: •Immunophenotyping •cell cycle and DNA content analysis •protein expression and bead based multiplex assays for signal transductionmolecules •soluble proteins cytokines and growth factors. Instrumentation includes: 1) three B-D FACSCalibur dual-laser cytometers capable of four color, 4 fluorescent parameter analysis, 2) a B-D LSR II four-laser cytometer capable of 13 color, 15 parameter analysis, 3) a B-D FACSArray dual-laser bioanalyzer capable of four color, plate-based multiplex assays, 4) A B-D FACSAria four-laser, high speed digital cell sorter capable of 15 color, 17 parameter analysis, 5)A B-D FACSAria II four-laser, high speed digital cell sorter capable of 15 color, 17 parameter analysis,7) a Miltenyi AutoMACs automated magnetic cell separation system. Wet laboratory space for sample preparation and incubators for cell storage following sorting operations. ---- 2 BD FACS Aria (SORP) sorters 13-15 colors 4 lasers. 2 BF LSR II (SORP)15-19 colors 4-6 lasers 3 BD FACSCaliber 2 laser four color 1 BD FACS Array Multiplex analyzer 1 Miltenyi AutoMACS magnetic cell sorter. 1 ProteinSimple NanoPro 1000 capillary electrophoresis instrument 1 ProteinSimple NanoPro 100 capillary electrophoresis instrument. 1 ProteinSimple NanoPro 100

Computational Services and Bioinformatics Facility Stanford University Beckman Center B062 279 Campus Drive Stanford, CA 94305 (650) 725-4483 consult@cmgm.stanford.edu View Website Primary Contact: Lee Kozar (650) 725-4483 kozar@stanford.edu Last Updated: 10/14/2011	Services offered: Computational - Bioinformatics Computational - Biostatistics Data Analysis DNA Analysis RNA analysis
This facility provides a wide variety of commonly used software for biomedical researchers. Software categories includes microarray analysis, sequence analysis, pathway analysis, graphics, visualization, statistical analysis, and more. Linux/Solaris servers and a multi-terabyte disk array are also available.

Flow Cytometry Core Facility University of Chicago 910 E. 58th Street Room 037 Chicago, IL 60637 (773) 702-9212 ucflow@gmail.com View Website Primary Contact: Ryan Duggan (773) 702-5582 rduggan@bsd.uchicago.edu Last Updated: 10/14/2011	Services offered: Assays and Measurements Cell Imaging Cell Sorting Data Analysis DNA Analysis F.I.S.H. Flow Cytometric Analysis Light Scattering Protocol Development/Clinical Trial Coordination Bead Based Analyte Quantitation Associations: CTSA Cancer Center ISAC MWACD
The Flow Cytometry Facility at the University of Chicago provides instrumentation and expertise in all parts of the cytometry process - from full service sample prep and data acquisition to training for self-service operation. We currently have state-of-the-art equipment including 4-laser 12 color BD LSRIIs, FACSAriaII cell sorters, Beckman Coulter MoFlo XDP, Amnis ImageStreamX, and BioRad BioPlex bead analyzer. ---- Find us on Facebook: facebook.com/ucflow Read our Flow Cytometry Tech Blog: ucflow.blogspot.com Follow us on Twitter: @ucflow ---- BD FACSAria II Beckman Coulter MoFlo XDP BD LSRII BD LSRFortessa Beckman Coulter Gallios BD FACSCanto BD FACSCalibur BD FACScan Amnis ImageStreamX BioRad BioPlex

Biostatistics Collaboration Center Northwestern University 680 N. Lake Shore Drive Suite 1400 Chicago, IL 60611 (312) 503-2288 bcc@northwestern.edu View Website Primary Contact: Thongsy Singvongsa Last Updated: 10/14/2011	Services offered: Computational - Biostatistics Data Analysis Computer Hardware/Software, Consultation
The Biostatistics Collaboration Center provides statistical support and expert consultation for the planning, design, development and execution of studies, and the analysis and interpretation of data, as well as the preparation of reports and manuscripts for basic science, clinical and epidemiologic research projects. ---- Additional services: research design, statistical analyses, limited database management. ---- Computer hardware, Statistical softwares

Experimental Pathology Core Laboratory University of Arkansas for Medical Sciences 4301 W. Markham St. Slot 845 Little Rock, AR 72205 (501) 686-8265 lhennings@uams.edu View Website Primary Contact: Leah Hennings, DVM (501) 526-7624 lhennings@uams.edu Last Updated: 10/14/2011	Services offered: Histology Immunohistochemistry Microscopy Necropsy Veterinary Services Digital Microscopy and Image Analysis Associations: CTSA
The Experimental Pathology Shared Resource Laboratory is a non-profit laboratory that provides Cancer Institute investigators with centralized, comprehensive histological services. The laboratory currently works with over 60 investigators. By providing a centralized laboratory, we offer convenient access to all services within one facility, save costs for investigators and the institution, increase efficiency of sample processing, eliminate duplication of services and equipment, and maintain stringent quality control standards. The laboratory director, consultants, and technicians have extensive experience in routine histology and immunohistochemistry involving both human and animal tissues. Tissue Work Order/IDT Services Provided Processing and embedding of tissues Frozen and paraffin-embedded tissue sections Routine hematoxylin/eosin staining A wide range of special histochemical stains Immunohistochemistry Sectioning of tissues for molecular studies and laser capture dissection Aperio digital slide scanning, brightfield and Fluorescence Image analysis Veterinary Pathology Necropsy Histopathology *Technical ---- Tissue-Tek VIP E300 Tissue Processor Fisher-Scientific Laboratory Refrigerator So-Low Ultra-Low Freezer Olympus B201 Dual-Head Microscope Microm HM505E Cryostat TBS Paraffin Dispenser Microm HM325 Microtome Tissue-Tek TEC Embedding Center Microm DS50 Slide Stainer Tissue-Tek Glass Coverslipper Chemicon Advanced Tissue Arrayer Computer - Aperio System Aperio System Aperio Scanscope CS and Scanscope FL

Protein Production Group University of Kansas, Lawrence Del Shankel Structural Biology Center 2034 Becker Drive Lawrence, KS 66047 (785) 864-4306 gao@ku.edu View Website Primary Contact: Dr. Philip Gao (785) 864-1825 gao@ku.edu Last Updated: 10/14/2011	Services offered: 2D-PAGE Cell Culture Molecular Construct Services Protein Extraction/Purification SDS-PAGE Spectroscopy Surface Plasma Resonance (SPR) Tissue Culture Western Blot Large-scale protein production Associations: COBRE ABRF
The COBRE Protein Production Group (PPG) focuses on the cloning, expression and purification of prokaryotic and eukaryotic proteins for COBRE and other investigators in Kansas and the nation. The laboratory maintains a variety of equipment to support the production of properly folded proteins in quantities suitable for structural studies (X-ray and NMR), functional studies (catalytic or biological), label-free binding studies (SPR) and/or high throughput (HTP) screening studies. ---- The lab provides training in any of the techniques it employs, including cloning, mutagenesis, protein expression in E. coli or insect cells, and protein purification via automated FPLC. Access to facilities is also provided for qualified users. ---- Incubators(3), thermocyclers(2), 1D, 2D and preparative electrophoresis, centrifuges(4), cell disruptor, automated FPLCs(3), Biacore 3000, UV-vis, Nanodrop, relevant small equipment; close association to high-field NMR, analytical proteomics (Mass spec), high-throughput screening, and X-ray crystallography labs.

Genetic Resources Core Facility (GRCF) BioRepository and Cell Center Johns Hopkins University 600 North Wolfe Street Blalock 1001A Baltimore, MD 21287 (410) 955-3320 biorepository@jhmi.edu View Website Primary Contact: Cameron Marlow (410) 614-5201 cmarlow2@jhmi.edu Last Updated: 10/14/2011	Services offered: BioBanking Cell Culture Tissue Culture cell line distribution
The Cell Center was established in 1989 to facilitate basic scientific research by providing expertise and service in all aspects of mammalian cell culture. Our primary service is establishing immortalized cell lines from mononuclear cells isolated from study participants' peripheral blood (EBV transformations). Other services include:The establishment and/or growth of mammalian cells,Repository services (banking and shipping samples), Consulting/training in cell culture. The Cell Center is a member of ISBER (the International Society for Biological and Environmental Repositories) and adheres to ISBER's Best Practices for Repositories.

Genomics Core Facility Pennington Biomedical Research Center 6400 Perkins Road Baton Rouge, LA 70808 (225) 763-0257 gcf@pbrc.edu View Website Primary Contact: Susan Newman (225) 763-0255 Susan.Newman@pbrc.edu Last Updated: 10/14/2011	Services offered: Computational - Bioinformatics Data Analysis DNA Analysis Genomics Library Services Microarray PCR Arrays Real-time qPCR RNA Integrity Sequencing - Capillary Sequencing Sequencing - DNA Sequencing Sequencing - Next-Gen Sequencing Western Blot Associations: COBRE ABRF
Mission: to provide next-generation sequencing for RNA and DNA analysis, DNA sequencing, DNA fragment analysis, qualitative and quantitative analysis of DNA, protein, and RNA samples, quantitative PCR, microarray services, robotics, and bioinformatics services. The Genomics Core Facility (GCF) provides training and consultation for sequence analysis, real-time PCR, and microarray analysis. The Genomics Core Facility seeks to achieve high quality and cost effective research data production by providing research services, sharing instrumentation, and providing the expertise of facility personnel to the research community. The laboratory provides next-generation sequencing services including resequencing, gene expression, small RNA, methylation, and ChIP analysis using Life Technologies SOLiD sequencer and DNA sequencing and fragment analysis with two 3130XL sixteen capillary genetic analyzers. Next-generation data storage and analysis is provided through a contract with Geospiza. Four Applied Biosystems 7900HT Sequence Detection Systems equipped with 96 well, 384 well, and low density array blocks perform quantitative PCR. Microarray service is provided using the Illumina Beadarray scanner. Spotfire software is used for analysis of microarray data. Two pipetting robots, a Becton Dickinson Biomek FX and a Perkin Elmer MultiProbe II, are available for robotic liquid handling. These instruments facilitate high-throughput pipetting of 384 well format plates. They can be programmed for large pipetting projects. Two Agilent 2100 Bioanalyzers are used for protein, DNA, and RNA analysis and quantitation. A NanoDrop Spectrophotometer and a Qubit fluorometer are used for RNA and DNA quantification. An Odyssey Infrared Imager uses direct infrared fluorescence detection for western blot analysis. For large DNA extraction and purification projects, a Qiagen AutoPure robot is available. Three computer workstations are provided for sequence analysis and alignment, PCR primer design, and RT-PCR data analysis. ---- RNA & DNA quantity and quality analysis, Licor Odyssey Western and in-cell Western imaging, Seahorse XF-24 physiological measurement of extracellular metabolites ---- Applied Biosystems- 2 3130XL Genetic Analyzers 4 7900HT Sequence Detection Systems 1 SOLiD Next Generation Sequencer 5500 IKA Ultraturrax Tube Drive Covaris S2 System Genomic Solutions HydroShear Illumina microarray system Becton Dickinson Biomek FX Perkin Elmer MultiProbe II Qiagen-AutoPure robot Qiacube robot Two Agilent 2100 Bioanalyzers NanoDrop Spectrophotometer Odyssey Infrared Imager Stratalinker 1800

Protein Structure Laboratory University of Kansas, Lawrence Del Shankel Structural Biology Center 2034 Becker Drive Lawrence, KS 66047 (785) 864-3772 swlovell@ku.edu View Website Primary Contact: Dr. Scott Lovell (785) 864-3772 swlovell@ku.edu Last Updated: 10/14/2011	Services offered: Single Crystal X-ray Pattern Analysis X-Ray Crystallography High throughput protein crystallography Associations: COBRE
The mission of the Protein Structure Laboratory (PSL) is to provide investigators with state-of-the-art instrumentation, facilities, and expertise for protein crystallization, X-ray data collection, data analysis, and structure solution and refinement from an a la carte menu. Laboratory staff provide advice and consultation, training, access to facilities for experienced crystallographers, and a range of services from crystal growth to full structure solution and refinement. ---- Rigaku R-axis++ generator; access to synchrotron beam 17 at APS; fully-equipped protein crystallization lab with various incubators and constant temperature room, computerized microscope with CCD camera.

UVM Center for X-Ray Crystallography University of Vermont Given Building - Rm E312 Burlington, VT 05405 (802) 656-9532 mrould@uvm.edu View Website Primary Contact: Mark Rould (802) 656-9532 mrould@uvm.edu Last Updated: 10/14/2011	Services offered: Molecular Imaging X-Ray Crystallography Associations: NERTRN
Facilities are available on a fee-for-service basis for robotic set-up of crystallization trials (for protein and/or DNA/RNA samples) and for collection of x-ray diffraction data from macromolecular crystals. (Note: This facility is not equipped to collect diffraction data from crystals of 'small molecules' ~<1000 Daltons MW.)

Dartmouth Electron Microscope Facility 7605 Remsen Hanover, NH 03755 (603) 646-1039 charles.p.daghlian@dartmouth.edu View Website Primary Contact: Charles Daghlian (603) 646-1039 charles.p.daghlian@dartmouth.edu Last Updated: 10/14/2011	Services offered: Electron Microscopy Spectroscopy scanning electron microscopy Associations: Cancer Center COBRE INBRE NICL
This facility provides scanning (conventional and FEG, ESEM, cryo and EDS, EBSP and direct-write E-beam lithography) and transmission (conventional and FEG and EDS) electron microscopy, scanned probe microscopy (AFM and STM), Raman confocal light microscopy and nanoindentation service and training to the Dartmouth community. Outside use as schedule permits. ---- JEOL JEM1010 TEM, LaB6 emitter FEI Tecnai T20FEG TEM FEI XL-30 ESEM FEG WiTEC CRM200 Raman confocal light microscope DI Dimension 3100 AFM DI Multimode AFM Hysitron Ubi-1 nanoindenter

Mass Spectrometry, Metabolomics and Proteomics Facility University of Illinois at Chicago 835 S. Wolcott Ave MSB E-125, Mail Code 937 Chicago, IL 60612 (312) 996-7602 proteomics@uic.edu View Website Primary Contact: Larry Helseth (312) 996-7602 helseth@uic.edu Last Updated: 10/14/2011	Services offered: Computational - Bioinformatics Mass Spectrometry Proteomics Preparative isoelectric focusing, Metabolomics Associations: Cancer Center ABRF MWACD
We are the Chicago area core proteomics facility, supporting researchers at the University of Illinois, Northwestern University and The University of Chicago. Our facility offers state of the art protein quantitation, protein identification and molecular weight determination using mass spectrometry. We accept sample submissions from academic researchers outside the Chicago area as well as from industry. We offer preparative isoelectric focusing, off-line HPLC fractionation, and other services. We host an annual workshop near the end of summer which is open to investigators outside the Chicago area. We also offer metabolomics and small molecule mass spectrometry services. Please visit our web site for more information then give us a call or e-mail us at proteomics@uic.edu with any questions. ---- We have developed the capability for high throughput protein IDs (8~15 minutes per run instead of 60~90 minutes per run) by adapting our Agilent Chip Cube to run on our Thermo instruments (currently used with our Orbitrap Velos Pro and our LTQ-FT Ultra). ---- Thermo Orbitrap Velos Pro Thermo Scientific LTQ FT Ultra Thermo Scientific LTQ Agilent 6410 QQQ (2) Applied Biosystems, Inc. 4700 Proteomics Analyzer Applied Biosystems, Inc. Voyager DE Pro Waters Waters SYNAPT Q-Tof Shimadzu LCMS IT-TOF Agilent 1946A LC-MSD Single Quad Agilent 3100 OFFGel Fractionator Thermo Scientific LCQ Classic Thermo Scientific TSQ Quantum JEOL GCMate II Magnetic Sector GC Mass Spectrometer Agilent 3100 OFFGel Preparative Isoelectric Focusing Apparatus

MicroCT Imaging Facility University of Connecticut Health Center New England Musculoskeletal Institute Department of Orthopaedic Surgery Farmington, CT 06034-4037 (860) 679-4976 dadams@nso.uchc.edu View Website Last Updated: 10/14/2011	Services offered: Cell Culture Cell Imaging Cell Sorting Clinical Imaging - Small Animal (X-ray, Ultrasound, microCAT, MRI) Computational - Application Development Computational - Bioinformatics Computational - Biostatistics Computational - High Performance Computing Confocal Microscopy Electron Microscopy Fabrication Flow Cytometric Analysis Histology In Situ Hybridization Laser Capture Microdissection Microarray Microscopy Surgical Services Associations: NERTRN
The UConn Health Center MicroCT Imaging Facility provides core services of three-dimensional imaging and quantitation of tissue structure using high-resolution X-ray computed microtomography (microCT). Resolution of structural features below 10 mm allows for accurate and precise evaluation of trabecular and cortical bone architecture, density, and porosity of bones from mice, including femora, tibiae, vertebrae, humeri, ulnae, calvaria, mandibles, teeth, and ectopic bone formation. Data obtained from microCT analyses are helpful toward identifying and quantitating definitive phenotypes in transgenic and knockout mouse models, as well as the skeletal effects of hormonal deficiency (e.g., ovariectomy) and pharmaceutical agents. Hydroxyapatite-phantom calibrated measurements of true volumetric density (mg/cm3) provide accurate measurements of mineralization shifts in these animal models, complementing rapid imaging modalities afforded by micro-DXA (Piximus) and radiography (Faxitron), which are also available. ---- Scanco uCT40 Scanco VivaCT40 Faxitron LX-60 IVIS Spectrum CSM nanoindenter Bose Electroforce 3200

Genomics and Epigenomics Shared Resource Georgetown University Lombardi Comprehensive Cancer Center 3900 Reservoir Rd NW, Research Building, Room E401 Washington, DC 20057 (202) 687-2283 hwr@georgetown.edu View Website Primary Contact: Habtom Ressom (202) 687-2283 hwr@georgetown.edu Last Updated: 10/14/2011	Services offered: Copy Number Variation (CNV) DNA Analysis Genomics Genotyping Microarray Nucleic Acid Extraction PCR Arrays Real-time qPCR RNA analysis RNA Integrity Sequencing - Capillary Sequencing Sequencing - DNA Sequencing Sequencing - Pyrosequencing Associations: Cancer Center ABRF
The Genomics & Epigenomics Shared Resource (GESR) provides services for various high throughput experiments including gene expression profiling, SNP genotyping, CNV analysis, DNA methylation, microRNA expression profiling, siRNA screening, DNA/RNA isolation, RNA quality assessment, DNA plating and assay preparation, DNA sequencing, and fragment analysis. GESR uses state-of-the-art instruments and assays to provide these services. These include Affymetrix Microarray System, Agilent Microarray System, Applied Biosystems TaqMan assays, Illumina BeadXpress, Qiagen PyromarkMD, Qiagen Qiacube, Agilent Bioanalyzer, Beckman Multimek NXP Robotic Liquid Handling System, and Applied Biosystems Sequence Analyzer. In addition, GESR houses several user-operated specialized instruments for which the shared resource personnel provide technical support and training.

Genome Sciences, Scientific Services Department The Jackson Laboratory The Jackson Laboratory 600 Main Street Bar Harbor, ME 04609 (207) 288-6219 lucy.rowe@jax.org View Website Primary Contact: Lucy Rowe lucy.rowe@jax.org Last Updated: 10/14/2011	Services offered: Data Analysis DNA Analysis Genomics Genotyping Library Services Nucleic Acid Extraction Sequencing - DNA Sequencing High Throughput Sequencing (Illumina) library prep from DNA, with or without sequence capture Associations: NERTRN Cancer Center
DNA Sequencing (ABI3730), genotyping with SNP or SSLP technology, genome scans for genetic mapping and breeder selection, genetic map building, custom genotyping projects, high quality genomic DNA available from inbred mouse strains, custom high throughput DNA prepapration for in-house genotyping applications, library construction from DNA for Deep Sequencing, targeted sequence enrichment for Deep Sequencing, consultation and data alaysis. Some services are limited to in-house clients, others available to outside - see http://jaxmice.jax.org/services/index.html Genome Science Services

Core Synthesis Facility North Dakota State University 156 Dunbar Hall NDSU Dept-2735, PO Box 6050 Fargo, ND 58108 (701) 231-8322 yonghua.yang@ndsu.edu View Website Primary Contact: Yonghua Yang, Ph.D. (701) 231-8322 Yonghua.Yang@ndsu.edu Last Updated: 10/14/2011	Services offered: Mass Spectrometry Nuclear Magnetic Resonance (NMR) Spectroscopy Organic Synthesis Associations: COBRE
The Core Synthesis Facility (CSF) at the Center for Protease Research (CPR) was established in April 2008 with funding from an NCRR-NIH-COBRE grant. The CSF is aimed towards assisting principal investigators in the COBRE program, by providing synthetic and analytical services. We are equipped with modern synthetic technologies and state of the art instrumentation. We specialize in the synthesis of small molecules for biological applications. We also offer quantitative and qualitative analysis of substrates using HPLC and LC-MS. ---- VAC Solvent Purification System Waters HPLC System Teledyne Isco Combi-flash Chromatography CEM Microwave Reactor Buchi Kugelrohr Distillation Oven Miniblocks XT parallel synthesis reactor Karl-Fischer Titrator

DNA Analysis Facility on Science Hill Yale University 21 Sachem Street ESC Room 150 New Haven, CT 06511 (203) 432-7394 dnanalysis@yale.edu View Website Primary Contact: Carol Mariani (203) 432-7394 carol.mariani@yale.edu Last Updated: 10/14/2011	Services offered: DNA Analysis Genotyping Sequencing - Capillary Sequencing Sequencing - DNA Sequencing
Medium Throughput Facility offering DNA Sequencing and Fragment Analysis for AFLP's & Microsatellites. Our prices are extremely competitive and our staff is especially knowledgable in the fields of Conservation & Population Genetics. ---- ABI 3730 XL PCR Machines NanoDrop/Qubit DNA Quantification

Electron Microscopy Service University of Illinois at Chicago Room 110, SES, M/C 337 845 West Taylor St Chicago, IL 60607 (312) 996-1227 nicholls@uic.edu View Website Primary Contact: Alan Nicholls (312) 996-1227 nicholls@uic.edu Last Updated: 10/14/2011	Services offered: Electron Microscopy Spectroscopy Associations: MWACD
The Electron Microscopy Service (EMS) is a central facility offering access to scanning (SEM), transmission (TEM) and scanning transmission (STEM) electron microscopes as well as Surface Analysis (X-Ray Photon Spectroscopy and Raman Spectroscopy) on a pay-per-use basis. Investigators are encouraged to learn how to operate the instrumentation themselves, however, the EMS staff are available to carry out experiments for those investigators who only occasionally require electron microscopy service. The EMS instrumentation is also available to investigators from outside UIC. ---- Specimen preparation services for both life science and materials science electron microscopy ---- JEOL JEM-ARM200CF TEM/STEM JEOL JEM-3010 TEM JEOL JEM-1220 TEM JEOL JSM-6320F SEM Hitachi S-3000N SEM Kratos AXIS-165 XPS

Systems Biology Core Facility University of Colorado Denver 12700 East 19th Avenue B-146 Research 2 Room 10480D Aurora, CO 80045 (303) 724-7244 karen.jonscher@ucdenver.edu View Website Primary Contact: Karen Jonscher (303) 724-7244 karen.jonscher@ucdenver.edu Last Updated: 10/14/2011	Services offered: 2D-PAGE Assays and Measurements BioBanking Biochemical Analysis Computational - Bioinformatics Data Analysis Gel Chromatography Mass Spectrometry Outreach PCR Arrays Proteomics Protocol Development/Clinical Trial Coordination Real-time qPCR SDS-PAGE Sequencing - Protein Sequencing Western Blot metabolomics, protein array Associations: MWACD CTSA ABRF WCASRD
The Systems Biology Core Facility provides open-access to qPCR and protein array instrumentation, develops cutting-edge proteomics assays, both discovery- and validation-based, and assists with metabolic profiling of selected metabolites. We serve researchers at the University of Colorado Denver, as well as investigators in labs throughout the nation and across the world. ---- We work with investigators to carefully plan experiments. We can either analyze prepared samples (protocols are available on our website) or take samples through the entire proteomics workflow. We provide collaborators with detailed results reports and work with them to mine the data for informative results. ---- Agilent Ultra quadrupole ion trap with Agilent NanoLC AB Sciex 4000 QTRAP with Eksigent nanopump Biorad qPCR

Ambry Genetics Ambry Genetics 100 Columbia # 200 Aliso Viejo, CA 92656 (949) 900-5500 quotes@ambrygen.com View Website Primary Contact: Jeff Owen (949) 900-5542 jowen@ambrygen.com Last Updated: 10/14/2011	Services offered: Assays and Measurements Computational - Bioinformatics Copy Number Variation (CNV) Cytogenetics DNA Analysis F.I.S.H. Genomics Genotyping Library Services Microarray PCR Arrays Sequencing - Capillary Sequencing Sequencing - DNA Sequencing Sequencing - Next-Gen Sequencing Sequencing - Pyrosequencing Sequence Capture
Ambry Genetics is uniquely positioned to assist you in your genomic research projects. Ambry has the expertise and technology to perform next generation sequencing, exome sequencing ,genotypying, microarrays and bioinformatics. Our dedicated staff of MD and PhD scientists are always available for consultation on your project. ---- Illumina HiSeq 2000, Illumina MiSeq, Illumina GA IIx, Ion Torrent PGM, Agilent microarray scanner, Roche microarray scanner, Biotage pyrosequencer, Illumina BeadXpress, ABI 3730xl, RainDance Thunderstorm and RDT 1000.

Genetic Resources Core Facility Johns Hopkins University Johns Hopkins University 600 North Wolfe Street, Blalock 1032 Baltimore, MD 21287 (410) 614-3255 bdaily@jhmi.edu View Website Primary Contact: Barbara Daily (410) 614-3255 bdaily@jhmi.edu Last Updated: 10/14/2011	Services offered: BioBanking Cell Culture DNA Analysis Genomics Genotyping Molecular Library Services Nucleic Acid Extraction oligo synthesis Real-time qPCR Sequencing - DNA Sequencing Sequencing - Next-Gen Sequencing Sequencing - Pyrosequencing Support Services - Central Laboratory Supply Tissue Culture Whole Genome Sequencing, Methylation Studies, Biorepository, Gene Synthesis Associations: ABRF
The Genetic Resources Core Facility (GRCF) is a JHU service center with six divisions. Collectively, these divisions produce a number of products and services to aid researchers performing studies in molecular biology and genetics. It is our mission to provide solutions to the everyday challenges of laboratory research. Additionally, the GRCF sponsors various educational programs, product seminars and an annual symposium ---- Illumina Bead ArrayTM Technology Applied Biosystems 7900HT PCR System Applied Biosystems 3730XL DNA Analyzer QIAGEN Pyromark24 System Applied Biosystems 7900HT MycoDTect DNA-array (Greiner, Bio-One) Illumina Genome Analyzer IIx Taqman 7900HT Real Time PCR

Structural Biology Resource Center Rockefeller University 1230 York Ave RRB 327 New York, NY 10065 (212) 327-7429 doren@rockefeller.edu View Website Primary Contact: Deena Oren (212) 327-7429 doren@rockefeller.edu Last Updated: 10/14/2011	Services offered: X-Ray Crystallography
The Structural Biology Resource Center (SBRC) is home to state-of-the-art X-ray equipment for the determination of the three-dimensional structures of biological macromolecules. The Center houses the Rigaku/MSC microMax 007HF generator equipped with Varimax optics that can be adjusted for small samples (300 micron), X-stream cryosystems and two RaxisIV++ detectors. The center also has a UVEX (Jan Scientific) microscope for identifying protein crystals of any size. ---- Based on availability, scientists from external not-for-profit research institutions may access the services of the Center. Fees for non-Rockefeller use of the instrumentation are listed on our website. ---- Rigaku MM007HF/HR R-Axis IV++ Phoenix Formulator

Data Analysis Core 1 Washington St Blacksburg, VA 24061-0477 (540) 231-2777 dac@vbi.vt.edu View Website Primary Contact: Robert Settlage (540) 231-2777 rsettlage@vt.edu Last Updated: 10/14/2011	Services offered: Computational - Application Development Computational - Bioinformatics Computational - Biostatistics Computational - High Performance Computing Copy Number Variation (CNV) Data Analysis
VBI's Data Analysis Core (DAC) is a dedicated multi-user resource that provides state-of-the-art data analysis support for biologically related research. The DAC was created to remove data analysis as an obstacle to performing the highly complex and data-intensive experiments required to solve modern problems in life science research. We have experienced staff available to provide guidance and support in designing and executing on properly powered and controlled experiments. Services At the DAC, a wide range of services are available to solve the data analysis bottleneck. We offer services ranging from training and self-serve workstations preloaded with necessary software to full service project support. Next generation sequencing projects include the analysis, assembly, and annotation of genomic sequence information including protein function prediction, variant/allele discovery, and expression analysis (CHIP-seq, RNA-seq, DNA-reseq, SNP-seq, and others). For gene expression (Affymetrix or custom), we offer statistical expression analysis, normalization, quality analysis, filtering customized to the experimental design, hierarchical clustering, and, when appropriate, function analysis. Custom array design for both oligonucleotide and proteomics arrays as well as target design for deep sequencing experiments are also available. For proteomics, we offer statistical expression analysis, pathway and GO analysis. All services result in manuscript-ready figures and text.

Protein Structure Core Facility University of Nebraska Medical Center 985819 Nebraska Medical Center Omaha, NE 69198-5819 (402) 559-6647 pscf@unmc.edu View Website Primary Contact: Laurey Steinke (402) 559-5176 lsteinke@unmc.edu Last Updated: 10/14/2011	Services offered: Outreach Sequencing - Protein Sequencing Amino acid analysis Associations: ABRF
The Protein Structure Core Facility operates in the department of Biochemistry and Molecular Biology, on the UNMC campus, Omaha Nebraska. It has been operating since 1988. The facility performs protein sequencing, amino acid analysis and peptide mapping on a fee-for-service basis. It is located on the first floor of the Durham Research Center. The facility is partially supported by funds from the Nebraska Research Initiative. Use of the facility is not limited to the University system. ---- The equipment includes an Applied Biosystems Procise protein sequencer, a Hitachi 8800 Amino Acid Analyzer and a Michrom MAGIC HPLC equipped with a Diode Array Detector.

Viral Vector Core Facility Maine Medical Center Research Institute 81 Research Drive Scarborough, ME 04074 (207) 396-8269 chandn@mmc.org View Website Primary Contact: Nancy Chandler-Conrey, MS (207) 396-8269 chandn@mmc.org Last Updated: 10/14/2011	Services offered: Virology Recombinant viral vector Associations: NERTRN COBRE
The Viral Vector Core was established at MMCRI in 2004. The objective of the core is to provide high quality viral vector services to support research performed at MMCRI as well as for outside institutions. Viral vectors are produced in a dedicated BL2 facility within the MMCRI building. Vector systems currently being used include adenovirus, retrovirus and lentivirus. The Core operates on a fee-for-service basis for both MMCRI and external investigators.

Center for Advanced Molecular Imaging (CAMI) Northwestern University Evanston, IL 30208 (847) 467-3306 cami@northwestern.edu View Website Primary Contact: Alex Waters (847) 467-3306 EAlexWaters@northwestern.edu Last Updated: 10/14/2011	Services offered: Cell Culture Cell Imaging Computational - High Performance Computing Confocal Microscopy Data Analysis Magnetic Resonance Imaging (MRI) Microscopy Molecular Imaging Optical Imaging Outreach Tissue Culture In vivo bioluminescence / fluorescence imaging Associations: Cancer Center MWACD
CAMI's goal is to provide a variety of imaging modalities and support facilities under a single roof. Our imaging modalities span nanometer to centimeter length scales (from scanning probe microscopy of cells to in vivo rodent imaging). Support facilities (tissue culture and animal prep) ensure streamlined experimental flow. ---- Animal prep area ---- 9.4T Bruker Biospec MRI 7T Bruker Pharmascan MRI IVIS Spectrum bioluminescence/fluorescence Upright & Inverted confocal microscope with 2 photon laser Veeco Bioscope 2

Virginia Bioinformatics Institute Virginia Tech Washington Street Blacksburg, VA 24061 (540) 231-1229 cores@vbi.vt.edu View Website Primary Contact: Robert Settlage (540) 231-5427 rsettlage@vbi.vt.edu Last Updated: 10/14/2011	Services offered: Computational - Application Development Computational - Bioinformatics Computational - Biostatistics Computational - High Performance Computing Copy Number Variation (CNV) Data Analysis DNA Analysis Genomics Genotyping Library Services Mass Spectrometry Microarray Molecular Construct Services Molecular Library Services Nucleic Acid Extraction Proteomics Real-time qPCR RNA analysis RNA Integrity SDS-PAGE Sequencing - Capillary Sequencing Sequencing - DNA Sequencing Sequencing - Next-Gen Sequencing Sequencing - Protein Sequencing Sequencing - Pyrosequencing
The Core Laboratory Facility (CLF) and Data Analysis Core (DAC) at VBI function as a multi-user resource dedicated to the development and application of various high-throughput technologies to aid in the discovery of biological macromolecules. These types of technologies are quite expensive to operate and maintain, making it difficult for individual laboratories to invest in this type of infrastructure. The model for the VBI CLF is to be a “one-stop” shop for these technologies providing researchers access to both the tools and our experienced staff. A central goal of the VBI CLF is to provide high quality data in a timely fashion and excellent customer service in a collaborative spirit with our customers. The VBI CLF currently provides analysis platforms for DNA (sequencing and genotyping), RNA (gene expression analysis) and proteins (proteomics). We also offer a selection of molecular biology applications (colony picking, cloning, DNA/RNA isolations). In addition to the ongoing application of existing technologies, the VBI CLF is also actively engaged in the development and testing of new technologies as needed. The VBI CLF is supported by a custom Laboratory Information Management System (LIMS) that provides an easy-to-use secure interface for sample submission and data retrieval. The combination of application and development enables the VBI CLF to remain a leader in helping researchers take a complete systems biology approach to their science. Beyond offering data generation services, the DAC offers investigators a method for analysis of the data generated in the CLF and elsewhere. The services offered through the DAC span the -Omics boundaries. Please see our website for more information. ---- Roche 454 GS FLX Sequencer, Illumina cBot, Illumina GAIIx Sequencer, Agilent Sure Select Platform, Processing of Nimblegen Seq-Cap Samples, Affymetrix Platform, Agilent Bioanalyzer, ABI 3730, ABI 3100, Biomek FX robot, Packard Multprobe liquid handling robot, Genetix QPixII robot, 2-D gel electrophoresis

Translational Genomics Core University of Connecticut Health Center Cell and Genome Sciences Building 400 Farmington Avenue Farmington, CT 06030 (860) 679-8706 ehager@uchc.edu View Website Primary Contact: Janet Hager (860) 679-5458 ehager@uchc.edu Last Updated: 10/14/2011	Services offered: Genomics Genotyping Library Services Microarray RNA Integrity Sequencing - Next-Gen Sequencing Associations: NERTRN
The Translational Genomics Core offers a variety of microarray and next-gen sequencing services for monitoring gene and microRNA expression, genotyping, and DNA methylation. ---- Illumina GAIIx Illumina HiSeq2000 Illumina BAR

CRC Informatics Core University of Vermont Given Courtyard 3 North UVM Burlington, VT 05405 (802) 656-8287 dhoward@uvm.edu View Website Primary Contact: Diantha Howard (802) 656-8287 dhoward@uvm.edu Last Updated: 10/14/2011	Services offered: Computational - Bioinformatics Data Analysis Associations: NERTRN CTSA
The CRC Informatics Core is available to investigators with protocols on the CRC and to those who request support for more limited services such as assistance with data entry or analysis. The Informatics Core Manager will assist the researcher in setting up the data entry process including Microsoft Access or REDCap database design, and in analyzing and interpreting the data. The Informatics Core facility consists of a file server accessible by PCs on the FAHC network or remote PCs through the FAHC "Gateway". Some of the software is accessible only from PCs on the CRC. Flat-bed (color) and document-feeding (black-and-white) scanners are available, with software for image manipulations and Optical Character Recognition. The CRC Informatics Core facility is a part of the Fletcher Allen Computer Network. All CRC PCs are configured with Microsoft Office Professional 2003 and Internet Explorer. A FAHC computer account, which the Informatics Core Manager can assist you in obtaining, is necessary to access the Informatics Core computers. Remote users access the CRC File Server securely by a VPN (Virtual Private Network) connection to the FAHC network. The Informatics Core manager will assist you in setting this up. CRC researchers may also store their research data on the Informatics Core file server. It will then be backed-up nightly and archived for a number of years.

W. M. Keck Center for Comparative and Functional Genomics University of Illinois at Urbana-Champaign 1201 W. Gregory Dr. Urbana, IL 61801 (217) 244-3930 markband@illinois.edu View Website Primary Contact: Mark Band Last Updated: 10/14/2011	Services offered: Assays and Measurements Computational - Bioinformatics Data Analysis DNA Analysis Genomics Genotyping Library Services Microarray Nucleic Acid Extraction Real-time qPCR RNA analysis RNA Integrity Sequencing - Capillary Sequencing Sequencing - DNA Sequencing Sequencing - Next-Gen Sequencing Associations: ABRF
The W.M. Keck Center is subdivided into three units by their research focus: High-Throughput Sequencing and Genotyping, Functional Genomics, and Bioinformatics. We offer Next-Gen Sequencing with our Illunima HiSeq for DNA, RNA-seq, CHIP-seq. Affymetrix, Illumina, Agilent and Nimblegen microarray services as well as microarray and sequencing bioinformatics. ---- Illumina HiSeq 2000 Illumina GXII ABI 3730 Affymetrix Illumina iScan Axon 4000B scanner ABI 7900 qPCR

Quantitative Methods Core UMass Medical School 55 Lake Avenue North ACC Building 7th Floor Worcester, MA 01655 (508) 856-8119 QMC@umassmed.edu View Website Primary Contact: Dr. Bruce Barton (508) 856-8191 bruce.barton@umassmed.edu Last Updated: 08/29/2011	Services offered: Quantitative Methods Analysis Associations: NERTRN CTSA
Services Available: Study Design, including General medical studies Clinical trials (Phase I - IV) Preclinical Quasi-experimental designs Retrospective/prospective studies Group - randomized designs Write statistical methods sections for funding applications Provide sample size and power calculations Plan and deploy interventions that require health informatics or mobile technologies Collect and manage your data Advice on resources available for conducting Health Services and Health Outcomes Research, such as Claims data (Medicaid, Medicare) Large survey databases (NHANES, BRFSS) Study Analysis Prepare reports and publications

Tissue Culture Facility/Media Center UMass Medical School 365 Plantation Street One Biotech Worcester, MA 01605 (508) 856-3745 louise.ohrn@umassmed.edu View Website Primary Contact: Louise Ohrn Last Updated: 08/29/2011	Services offered: Tissue Culture Custom Made Media Associations: NERTRN CTSA
Tissue culture services include but are not limited to: Tissue and cell culture Monoclonal antibodies production and purification Hybridoma production (in collaboration with investigator) Cell cloning (hybridoma and transfected cells) Routine maintenance cell lines in monolayer and suspension culture Cell production (monolayer, suspension and spinner culture) Preparation of specialized media and buffers Programmable cryopreservation of tissue or cell stocks for higher viability Preparation and freezing of cell stocks Centralized cell storage in liquid nitrogen Primary culture (e.g. from skin biopsy, vascular endothelium, or other tissues, done in collaboration with investigator) Mycoplasma testing

Viral Vector Core Facility UMass Medical School 381 Plantation Street Biotech V Worcester, MA 01605 (508) 856-3563 guangping.gao@umassmed.edu View Website Primary Contact: Dr. Guangpin Gao Last Updated: 08/29/2011	Services offered: Viral Vectors Associations: NERTRN CTSA
The Viral Vector Core (VVC) of the Gene Therapy Center uses cutting-edge technologies to create and produce a variety of high quality viral vectors. The goal of the VVC is to provide investigators with the most suitable and efficient gene transfer vectors for their research applications.

Zebrafish Core Facility UMass Medical School 55 Lake Ave North Worcester, MA 01655 (508) 856-1177 zebrafishcore@umassmed.edu View Website Primary Contact: Nathan Lawson (508) 856-1177 nathan.lawson@umassmed.edu Last Updated: 08/29/2011	Services offered: Animal Husbandry Genotyping Genotyping Associations: NERTRN CTSA
Microinjection, genotyping and husbandry of zebrafish available. Please see website for more details.

Mouse Phenotyping Center UMass Medical School 381 Plantation Street Biotech V, Suite 200 Worcester, MA 01605 (508) 856-6840 jason.kim@umassmed.edu View Website Primary Contact: Jason Kim (508) 856-6807 jason.kim@umassmed.edu Last Updated: 08/29/2011	Services offered: Molecular Imaging Ultrasonic Imaging Mouse Phenotyping Associations: NERTRN
The UMass Mouse Phenotyping Center (UMPC) is designed to provide the scientific community with sophisticated and standardized experimental tools for the purpose of investigating transgenic mouse models and understanding obesity, diabetes and its complications. The UMPC is composed of multidisciplinary group of investigators at the UMass Medical School and consists of the following Phenotyping Cores: Metabolism Core performs elegant and non-invasive metabolic experiments to assess insulin sensitivity, glucose/lipid/protein metabolism, body composition and energy balance in conscious mice. Analytical Core utilizes clinical chemistry analyzer and Luminex to perform high-throughput measurement of serum/tissue hormones, metabolites and cytokines known to affect metabolism. Cardiovascular Core applies a state-of-the-art imaging platform to non-invasively assess cardiac structure and function and vascular imaging to examine cardiovascular diseases. Retinopathy Core (coming soon)

High-Throughput Bioscience Center Stanford University 269 Campus Drive, CCSR Room 0133 Stanford, CA 94305 (650) 725-6002 desolow@stanford.edu View Website Primary Contact: David Solow-Cordero (650) 725-6002 desolow@stanford.edu Last Updated: 08/23/2011	Services offered: Assays and Measurements Cell Culture Cell Imaging Computational - Bioinformatics Computational - Biostatistics Data Analysis Microscopy Molecular Library Services Tissue Culture High-Throughput Screening Associations: Cancer Center ABRF
The High-Throughput Bioscience Center's mission is to provide researchers at Stanford with the ability to run high-throughput chemical, siRNA, cDNA, and high-content screens for the purpose of drug and/or target discovery. We have over 130,000 small molecules for compound screens, 15,000 cDNAs for genomic screens, and the siARRAY whole human genome siRNA library from ThermoFisher Scientific (formerly Dharmacon) targeting 21,000 genes. ---- - Molecular Devices ImageXpress Micro High-Content fluorescence microplate imager, with live cell and phase contrast/brightfield options - Caliper Life Sciences SciClone ALH3000, with integrated TwisterII robot, 198 position automated incubator, and reagent dispensers. Used for HTS of small molecules. - Agilent Bravo microplate liquid handler with 96- and 384-tip heads, integrated stackers, plate washer, reagent dispense and plate sealer. Used for siRNA screens. - Molecular Devices Analyst GT fluorescence, FP, TRF, HTRF, luminescence, and absorbance multimode microplate reader. - Molecular Devices FlexStation II 384 fluorescent plate reader with integrated pipeting head for kinetic reads, i.e. Ca+ mobilization. - Tecan Infinite M1000 fluorescence, FP, TRF, HTRF, luminescence, and absorbance multimode microplate reader. - Microplate Washers - Microplate Reagent Dispensers - Agilent VPrep 96-tip head pipeting station.

Molecular Phenotyping Sciences The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 (207) 288-6703 douglas.hinerfeld@jax.org View Website Primary Contact: Doug Hinerfeld douglas.hinerfeld@jax.org Last Updated: 08/22/2011	Services offered: 2D-PAGE Biochemical Analysis Cell Culture Cell Sorting Copy Number Variation (CNV) Data Analysis DNA Analysis Flow Cytometric Analysis Gel Chromatography Genomics Genotyping Mass Spectrometry Microarray Molecular Construct Services Molecular Library Services Nucleic Acid Extraction Protein Extraction/Purification Proteomics Real-time qPCR RNA analysis RNA Integrity SDS-PAGE Sequencing - Next-Gen Sequencing Western Blot Associations: NERTRN Cancer Center ABRF
Through Molecular Phenotyping Sciences, researchers access state-of-the-art gene expression and high-throughput sequencing technologies , nucleic acid isolation and quality assessment, gene targeting and transgenic construct design, protein analysis, mass spectrometry, advanced flow cytometric technology and a monoclonal antibody resource. ---- Bruker MALDI-TOF, AKTA FPLC, HPLC, Illumina HiSeq, Affymetrix, BD LSRII, BD FACS-Aria, BD FACS-scan, BD FACS-calibur, Agilent Bioanalyzers, Nanodrop.

Electron Microscopy Service The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 (207) 288-6322 lesley.bechtold@jax.org View Website Primary Contact: Peter Finger (207) 288-6337 pete.finger@jax.org Last Updated: 08/19/2011	Services offered: Electron Microscopy Protocol Development/Clinical Trial Coordination Immunogold Labeling Associations: NERTRN Cancer Center
The EM Service offers both SEM (Scanning electron microscopy) and TEM (Transmission electron microscopy) as well as sample preparation for both types of EM. The EM Service is equipped with a Hitachi S3000N Variable pressure Scanning electron Microscope and a JEOL 1230 Transmission electron Microscope, both with digital image capture capability.

Histology Service The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 (207) 288-6322 lesley.bechtold@jax.org Primary Contact: Lesley Bechtold (207) 288-6322 lesley.bechtold@jax.org Last Updated: 08/19/2011	Services offered: Histology Immunohistochemistry Protocol Development/Clinical Trial Coordination Tissue Processing, Sectioning and Staining Associations: NERTRN Cancer Center
The Histology Laboratory provides a centralized service for the preparation of murine tissues for light and fluorescent microscopy. Routine and specialized histological methods are available; including step and serial sectioning, routine and special stains, immunohistochemistry and cryo methods.

Necropsy Service The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 (207) 288-6322 lesley.bechtold@jax.org Primary Contact: Sue Grindle (207) 288-6321 sue.grindle@jax.org Last Updated: 08/19/2011	Services offered: Necropsy Protocol Development/Clinical Trial Coordination Associations: NERTRN
The Necropsy Service performs necropsies on mice from both production and research animal facilities for diagnostic pathology purposes as part of JAX's routine monitoring to ensure the health status of its mice. Mice may also be sent to us from research colonies for either surveillance purposes or as part of a research project in which specific tissues need to be harvested. Training is provided to researcher staff who wish to learn to perform their own necropsies. In addition to necropsies and tissue collection for histological processing, we can collect blood smears for staining and do fluid collection for PCR, hematology analysis and microbiology culturing. We also collect tumors for inclusion in the Tumor Database, photograph mice prior to necropsy and take X-rays as needed.

Clinical Assessment The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 (207) 288-6322 lesley.bechtold@jax.org Primary Contact: Sue Grindle (207) 288-6321 sue.grindle@jax.org Last Updated: 08/19/2011	Services offered: Clinical Assessment (Hematology, Clinical Chemistries, etc.) Data Analysis Associations: NERTRN
Clinical Assessment employs the latest technology to measure specific parameters in the blood and urine of mice for the purpose of phenotyping strain differences and for the detection of disease. Chemistry analysis is performed on a Beckman Synchron DXC, and Hematology analysis is performed on a Siemens Advia 2120. We utilize a systematic Quality Assurance program as an integral part of our operation. The primary focus of our Quality Control program is to ensure specificity, accuracy, precision and sensitivity of the tests offered by means of continuous surveillance of calibration, three levels of assayed human-based controls, and diligent maintenance of the analyzer. You can receive a copy of the control values with your results. We also run daily mouse controls utilizing the reference ranges established from the Tier 1 Project. ---- Beckman DxC Pro 600 Chemistry Analyzer Advia 2120 Hematology Analyzer

Imaging Sciences The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 (207) 288-6000 james.denegre@jax.org View Website Primary Contact: James Denegre (207) 288-6648 james.denegre@jax.org Last Updated: 08/16/2011	Services offered: Assays and Measurements Behavioral Phenotyping Cell Culture Cell Imaging Clinical Imaging - Small Animal (X-ray, Ultrasound, microCAT, MRI) Computational - Application Development Confocal Microscopy Cytogenetics F.I.S.H. Immunohistochemistry In Situ Hybridization Laser Capture Microdissection Microscopy Molecular Imaging Multiphoton Microscopy Optical Imaging Outreach Protocol Development/Clinical Trial Coordination Support Services - Biological photography/Photomicrography Tissue Culture Ultrasonic Imaging Associations: NERTRN Cancer Center
Imaging Sciences delivers light microscopy, cytogenetics, whole-animal imaging, and mouse behavioral, physiological and sensory phenotyping. Service capabilities include: multi-photon and single-photon confocal microscopy, nano -resolution microscopy, live-cell imaging, semi-automated widefield fluorescence microscopy, laser capture/microdissection, and digital slide scanning; G-banding, FISH and spectral karyotyping for multiple species; microCT, ultrasound, thermal imaging, and bioluminescent and fluorescent imaging; behavioral, physiological/ metabolic and sensory phenotyping. The Service also provides data analysis and statistics for phenotyping and extensive image analysis, including 3-D reconstruction, 3-D measurements, quantitation and morphometrics. The Service has expertise in all imaging modalities; experience with a wide variety of mouse models of disease and transgenic mouse models; and is prepared to assist the user in experimental design. ---- Comprehensive Cage Monitoring System (Columbus Instruments, Columbus, OH). Concorde MicroCAT II micro-computed tomography system, Siemens Medical Solutions USA (Malvern, PA). Vevo 770™ high-frequency ultrasound machine, VisualSonics, Inc. Thermal Imager, Thermogenic Imaging, Inc. (N. Bellerica, MA). Xenogen Lumina II bioluminescence and biofluorescence imaging system, Caliper Life Sciences (Hopkinton, MA). Leica TCS SP2 MP AOBS Spectral Confocal and Multiphoton Microscope with UV and IR lasers, Leica Microsystems (Bannockburn, IL). Leica TCS SP5 AOBS Spectral Confocal Microscope with UV laser, Leica Microsystems (Bannockburn, IL). Leica 4Pi confocal nano-resolution microscope, Leica Microsystems (Bannockburn, IL). Zeiss AxioObserver fluorescence microscope, Carl Zeiss Microimaging (Thornwood, NY). Spectral Karyotyping System, Applied Spectral Imaging (SanDiego, CA). NanoZoomer HT digital pathology slide scanner, Olympus America (Center Valley, PA). Arcturus XT Laser Capture Microdissection system, Applied Biosystems. Metamorph, Autoquant Amira and Bitplane software.

Equipment Repair Service The Jackson Laboratory 600 Main Street Bar Harbor, ME 04609 (207) 288-6275 jeff.forthofer@jax.org Primary Contact: Jeff Forthofer Last Updated: 08/16/2011	Services offered: Fabrication Support Services - Electronics & Fabrication Shop Support Services - Shared Instrumentation Oversight & Maintenance Associations: NERTRN
The Equipment Repair Service provides technical support and maintenance of the wide variety of shared scientific equipment and common equipment rooms. In-house repair of PI instrumentation is also available. Custom fabrication of new fixtures and devices, or modification of existing instrumentation is also offered as part of the service.

DNA Services Core Oregon Health and Science University 3181 SW Sam Jackson Park Rd. Richard Jones Hall, room 6550 Portland, OR 97239 (503) 494-2472 core@ohsu.edu View Website Primary Contact: Mariah Reeves (503) 494-2472 core@ohsu.edu Last Updated: 08/11/2011	Services offered: Data Analysis Genotyping Sequencing - Capillary Sequencing Sequencing - DNA Sequencing Associations: ABRF
DNA sequencing & fragment analysis is accomplished with our state-of-the-art, Applied Biosystems 3130xl, capillary fluorescence instrument. Several levels of service are available to match your needs and finances. We are happy to consult on your sequence assembly and analysis or other data processing, informatics, and database needs as well. ---- Applied Biosystems 3130xl, capillary fluorescence sequencer

COBRE for Skeletal Health and Repair - Molecular Biology Core Rhode Island Hospital 1 Hoppin St. Coro West, Suite 402A Providence, RI 02903 (401) 444-6604 RAlberg@lifespan.org View Website Primary Contact: Qian Chen, Ph.D. (401) 444-6604 RAlberg@lifespan.org Last Updated: 07/29/2011	Services offered: Assays and Measurements Atom Probe Tomography Biochemical Analysis Cell Culture Cell Imaging DNA Analysis Microscopy Molecular Imaging Multiphoton Microscopy Optical Imaging Phosphor Imaging Real-time qPCR RNA analysis SDS-PAGE Tissue Culture Western Blot Associations: COBRE
The COBRE for Skeletal Health and Repair in the Rhode Island Hospital enables clinicians and basic scientists, engineers and biologists to work side-by-side on multidisciplinary research, helping us to better understand cartilage and joint health mechanisms and develop strategies for the prevention and treatment of skeletal joint diseases. Specific Aims: -Provide sophisticated, state-of-the-art instrumentation in microscopic imaging and molecular analysis to different projects in the COBRE. -Provide technical support and expert consultation in tissue, cell, and molecular, analysis to multidisciplinary users including biologists, clinicians and engineers. -Provide training of cutting edge techniques in molecular and morphological analysis to investigators for achieving specific research objectives. -Facilitate collaborations among different laboratories and disciplines through utilizing common equipment and analyzing techniques. ---- A list of the major equipment available in the Core is listed as follows: -Nikon 90i Upright Research Microscope (fully automatic stage with digital deconvolution) -Nikon Eclipse TS-100F Inverted Microscope with Phase Contrast/FITC/RFP -Nikon Y-THR-L Tri-headed Teaching Microscope -Leica KL 1500 Dissection Microscope with Polaroid Camera -GloMax 20/20 Luminometer System + Printer -VisEn FMT Fluorescence In Vivo Imaging System -Luminex 200 System -Scanco three-dimensional (3-D) microcomputed tomography (µCT)-40 -Faxitron X-Ray Machine -Li-COR Odyssey Infrared Imager -UVP Biochemi System (Bioimaging System with Epichemi Darkroom) -Thermo Shandon Finesse Automatic Microtome and Heater Control Thermostat -Leica CM1800 Cryostat -Nanodrop ND100 -Thermo Biogate Spetrophotometer -Qiagen QIAxcel -Qiagen QIAgility -Bio-Rad CFX96 Real-time System -DNA Engine Opticon 2 -Verti 96 Well Thermal Cycler -Bio-Rad C-1000 Thermal Cycler -Bio-Rad S-1000 Thermal Cycler -Labnet Multigene Thermal Cycler -Labnet HERMLE 2400 Centrifuge -Isotemp Vacuum Oven Model 280A -Isotemp Hybridization Oven with Rotisserie Motor -Sorvall Legend X1R Centrifuge with Rotors -Sorvall Discovery 100SE Ultraspeed Centrifuge with Rotors -Fisher Bench-Top Centrifuge CL2 -Eppendorf Microcentrifuge 5424 (X3) -Orbital Microplate Shaker -VWR 12L Shaking Water Bath -Fisher Scientific PowerGen125 Homogenizer Package -BioRad Sub-Cell Electrophoresis Systems -Trans-blot Electrophoretic Transfer Cell Complete Unit -Packard Tri-Carb 2900TR Liquid Scintillation Analyzer -AGFA CP1000 Film Developer -Various 4 Degrees Refrigerator and Minus 20 and Minus 80 Freezers -Nuaire CO2 Water-Jacketed Incubators -Nuaire Class II Type A/B3 Biological Safety Cabinets

FACS and ES Cell Core Maine Medical Center Research Institute 81 Research Drive Scarborough, ME 04074 (207) 396-8314 arzigm@mmc.org View Website Primary Contact: Dr. Zack Wang wangz@mmc.org Last Updated: 07/13/2011	Services offered: Cell Sorting Associations: NERTRN COBRE
The cell separation branch of this core provides instrumentation and expertise for cell sorting, using FACS and MACS technologies. The ES cell branch of this core provides an institutional resource for the study of embryonic stem (ES) cells, having a dedicated ES cell culture facility equipped with tissue culture microscopes and a fluorescence microscope, tissue-culture hoods, CO2 incubators, LN2 storage, and ES cell specific reagents. For cell separation interests, this core provides two instrumentation resources: the FACSAria flow cytometer from BD Biosciences and the autoMACS separator from Miltenyi Biotec. The FACSAria is the first fixed alignment benchtop high-speed sorter. With its digital electronics and various pressures, it can process 70,000 cells per second. Other features include its fixed-optics, multi-well sorting and the capacity of analyzing 15 parameters simultaneously. The autoMACS from Miltenyi Biotec uses MAgnetic Cell Separation (MACS) technology. Magnetic Cell Separation utilizes highly specific isolation of magnetically labeled cells. The cells of interest are labeled, passed through a separation column and are separated from the unlabeled cells. The system is fast, efficient and completely compatible with the flow cytometers. This core provides technical support, training, and services for stem cell research. ES cell lines will be maintained with appropriate quality assurance in the facility. The technical support provided by the facility will include teaching of methods for the maintenance, transduction, and differentiation of the cells. The facility will provide short-term culture and manipulation of the cells for individual investigators. In addition, the core will provide ready access of ES cell reagents (RNAs, Genomic DNAs, and proteins) to individual investigators without either the need for specialized space, or the need to develop specialized technical skills required for ES cell work. The ES cell core will also provide technical support and training for human ES cell culture (NIH approved human ES cell lines: H1 and H9). A variety of mouse embryonic feeder (MEF) cells, inactivated and plated, is also available through this core.

Zebrafish Core Mount Desert Island Biological Laboratory Salisbury Cove, ME 04672 (207) 288-3605 cwray@mdibl.org View Website Primary Contact: Charles Wray Last Updated: 06/30/2011	Services offered: Animal Husbandry Transgenics Associations: INBRE
MDIBL operates numerous rooms of stand alone and rack driven zebrafish colonies. Expansion is underway

Confocal Imaging Core Mount Desert Island Biological Laboratory Mount Desert Island Biological Laboratory PO Box 35 Salisbury Cove, ME 04672 (207) 288-3605 achristie@mdibl.org Primary Contact: Christine Smith Last Updated: 06/30/2011	Services offered: Cell Imaging Confocal Microscopy Optical Imaging Associations: INBRE
MDIBL provides access to two single photon confocal microscopes: a Zeiss 510 Meta and a Olympus FV1000. Technicians provide training services to new users. Reservations can be made via an online sign up system.

Bioinformatics Mount Desert Island Biological Laboratory PO Box 35, Old Bar Harbor Rd. Salisbury Cove, ME 04672 (207) 288-3605 cmattin@mdibl.org View Website Primary Contact: Carolyn Mattingly cmattin@mdibl.org Last Updated: 06/30/2011	Services offered: Computational - Bioinformatics Computational - Biostatistics Associations: INBRE
The Maine INBRE Bioinformatics Core facilitates data management and analysis for comparative functional genomics research by providing: * The Comparative Toxicogenomics Database (http://ctd.mdibl.org) * Sequence Analysis Software * Access to biological databases * Capabilities for data storage The Core also supports communication networks for INBRE institutions to facilitate multi-center research and resource sharing and the confidential exchange of data.

Mouse Transgenic and In Vivo Imaging Core Maine Medical Center Research Institute 81 Research Drive Scarborough, ME 04074 (207) 885-8142 liawl@mmc.org View Website Primary Contact: Lucy Liaw liawl@mmc.org Last Updated: 06/30/2011	Services offered: Animal Husbandry Genotyping Magnetic Resonance Imaging (MRI) Stem Cell Procurement Transgenics mouse embryo cryopreservation and re-derivation Associations: NERTRN
The Mouse Transgenic Core Facility provides a series of high quality services related to mouse genetics, manipulation of mouse embryos, re-derivation and cryopreservation, and the stocking and distribution of shared mouse strains. Transgenic mouse lines are generated by microinjection, and chimeric mice are made by blastocyst injection of embryonic stem cells. In vivo small animal magnetic resonance imaging is performed with a Bruker PharmaScan 7T, 300MHz imager. Please contact Dr. Ilka Pinz (pinzi@mmc.org) for more information regarding MRI services."

Brown University Transgenic Core Brown University 70 Ship Street Providence, RI 02903 (401) 863-9544 Erin_Paul@brown.edu View Website Primary Contact: Erin Paul (401) 863-9544 Erin_Paul@brown.edu Last Updated: 06/21/2011	Services offered: Assisted Reproductive Technologies (Rodent IVF, ICSI) Cell Culture Embryo Cryopreservation & Recovery Services Surgical Services Transgenics Microinjection & Rederivation Associations: NERTRN COBRE
The Transgenic Mouse and Knockout Core generates transgenic animals for Brown University, affiliated hospitals and regional investigators. The facility includes stand-alone microinjection facility capable of handling both pronuclear and blastocyst microinjections. An ES cell facility is attached to the core. Its function is to perform gene-targeting manipulations with vectors supplied by investigators. The facility is responsible for all supporting activities, such as the preparation of feeder cells or the maintenance of necessary mouse colonies. The core also advises investigators on follow up breeding regiments and analysis of the transgenic animals. Other services include Embryo Cryopreservation and Rederivation. ---- http://www.brown.edu/Departments/Molecular_Biology/transgenic.html

Outcomes Measurement & Survey Core - OMSC Northwestern University 710 N. Lakeshore Drive Suite 729 Chicago, IL 60611 (312) 503-9804 e-hahn@northwestern.edu View Website Primary Contact: Elizabeth Hahn (312) 695-1329 e-hahn@northwestern.edu Last Updated: 03/23/2011	Services offered: Computational - Application Development Data Analysis Protocol Development/Clinical Trial Coordination Research Design Consultation Services Associations: MWACD
The mission of this core facility is to provide consultation and support for research that involves collecting, analyzing or interpreting self-report data in culturally diverse populations. The facility provides expertise on the best ways to measure outcomes derived by self-report, serves as a central resource for state-of-the-science instruments and measurement methods, and provides in-house support services for translation, collection and analysis of outcomes and survey data. •Research Design Consultation Services ◦Assistance with development of new protocols ◦Questionnaire/Survey selection and development ◦Recruitment strategies and assessment plans ◦Analysis methods •Programming & Analysis Services ◦Computer-based questionnaire administration tools ◦Scoring algorithms ◦Psychometric and statistical analysis and interpretation •Research Operations Services ◦Training for study interviewers ◦Multilingual study interviewers ◦Multilingual translations of questionnaires and surveys ◦Qualitative methods (focus groups, cognitive interviewing) ---- Analysis software on PCs , including SAS and SPSS for statistical analysis , Access and SQL Server for data management , BILOG , WINSTEPS , LISREL and Facets for psychometric analysis, Computer-based questionnaire administration tools, Facilitate IRB approval, Multilingual study interviewers, Programmers have SQL Server and supported client tools , Visual Studio.net 2003 , Visual Studio 6.0 , Access 97 , Access XP, Psychometric and statistical analysis and interpretation, Qualitative methods - focus groups , cognitive interviewing, Research Design Analysis methods, Research Design Questionnaire / Survey selection and development, Research Design Recruitment strategies and assessment plans, Scoring algorithms

Vanderbilt Antibody and Protein Resource (VAPR) Vanderbilt University Medical Center 892 PRB 2220 Pierce Ave Nashville, TN 37232 (615) 936-3092 robert.carnahan@vanderbilt.edu View Website Primary Contact: Robert Carnahan robert.carnahan@vanderbilt.edu Last Updated: 02/24/2011	Services offered: Biochemical Analysis Monoclonal Antibody Protein Extraction/Purification Associations: ABRF Cancer Center CTSA MWACD
The Vanderbilt Antibody and Protein Resource (VAPR) facility offers technical advice and services aimed at simplifying the process of generating, purifying, and characterizing monoclonal and polyclonal antibodies. We also offer a range of recombinant protein production and purification services in both bacterial and mammalian expression systems.

BU Microarray Resource Boston University BUSM 72 E. Concord, E605 Boston, MA 024118 (617) 414-1377 yurik@bu.edu View Website Last Updated: 02/21/2011	Services offered: Genotyping Library Services Microarray Associations: ABRF
Currently, we provide analysis of gene expression using the Affymetrix GeneChip platform. Services using the Illumina platform will be available soon. For more information go to the services section of our website. For both platforms users of the facility provide us with high quality samples and the Microarray Resource does the rest. We also offer analysis of RNA, DNA and Proteins using Agilent Bioanalyzer. We offer the same services to researchers outside of Boston University on an available-resource basis. All interested researchers are encouraged to contact the Microarray Resource to discuss potential projects or to ask any question about the use of microarrays. Dr. Alekseyev can provide advice on experimental design, RNA isolation methods, and just about any aspect of array hybridization. Dr. Lenburg can provide advice on experimental design and data analysis. Please note that we were able to reduce our pricing starting 12/16/2010. ---- Gene Chip system, Bead array reader

Proteomics Core Facikity Baylor College of Medicine One Baylor Plaza ABBR Building room R503 Houston, TX 77030 (713) 798-2326 deane@bcm.edu View Website Primary Contact: Dean Edwards (713) 798-2326 Last Updated: 02/21/2011	Services offered: 2D-PAGE Data Analysis Mass Spectrometry Monoclonal Antibody Protein Extraction/Purification Proteomics Sequencing - Protein Sequencing protein and antibody arrays Associations: Cancer Center
Multiple proteomic platforms. MS based identification of protein complexes and PTMs. Protein interactomes by BiFC technologies. Protein profiling differential expression by 2D gels and liquid chromatogrpahy, antibody and protein arrays and Luminex beads. Custom baculovirus recombinant protein expression/production and monoclonal antibody development and production.

Microchemistry and Proteomics Core Laboratory Memorial Sloan Kettering Cancer Center 1275 York Avenue New York, NY 10065 (212) 639-2405 h-bromage@ski.mskcc.org Primary Contact: Hediye (212) 639-2405 h-bromage@ski.mskcc.org Last Updated: 02/20/2011	Services offered: Mass Spectrometry Peptide Synthesis Proteomics N-terminal Sequence Analysis Associations: ABRF
Proteomics Analysis, Peptide Synthesis

Genome Technology Center New York University 550 First Avenue New York, NY 10016 (212) 263-8048 jiri.zavadil@nyumc.org View Website Last Updated: 02/20/2011	Services offered: DNA Analysis Genomics Microarray Sequencing - DNA Sequencing Sequencing - Next-Gen Sequencing
NYULMC's Genome Technology Center provides an environment that facilitates crosstalk between researchers from different fields such as biology, chemistry and bioinformatics. The NYULMC Genome Technology Center provides access to highly specialized equipment and unique expertise, including: 1. high-throughput genomic and computational methodologies, such as gene and microRNA expression profiling and functional genomics experiments (protein:chromatin DNA interactions) 2. genome-wide array- and quantitative PCR-based expression profiling 3. high-capacity chromatin structure studies (ChIP-on-chip) 4. DNA analyses (whole genome SNP genotyping, high-resolution chromosome copy number analysis, high-capacity quantitative PCR and pyrosequencing) 5. consultations and 1-on-1 assistance with issues of experimental design 6. storage, annotation, processing, and analysis of data 7. educational activities to assist the user community with various aspects of genomics, bioinformatics and other fields of modern biology

Next-Generation Sequencing and Expression Analysis Core University at Buffalo, SUNY New York State Center of Excellence in Bioinformatics and Life Sciences 701 Ellicott Street Buffalo, NY 14203 (716) 881-7514 ubnextgencore@buffalo.edu View Website Primary Contact: Jennnifer Jamison, M.S. (716) 881-7514 jjamison@buffalo.edu Last Updated: 02/20/2011	Services offered: Assays and Measurements Data Analysis DNA Analysis Genomics Library Services Microarray Molecular Library Services RNA Integrity Sequencing - DNA Sequencing Sequencing - Next-Gen Sequencing Sequencing - Pyrosequencing Associations: ABRF
The University at Buffalo Next-Generation Sequencing and Expression Analysis Core is located on the Buffalo Niagara Medical Campus at the New York State Center of Excellence in Bioinformatics and Life Sciences. The core provides services to SUNY at Buffalo, Roswell Park Cancer Institute, WYN Businesses, and Institutions nationwide. Next-Gen sequencing services are provided using the Roche/454 Genome Sequencer FLX and illumina HiSeq 2000 platforms. The UB Next-Gen and Expression Core also provides expression analysis services on all Affymetrix platforms including 3'expression, miRNA, exon, and gene arrays.

MS Core Facility Oregon State University 1011 ALS Bldg Corvallsi, OR 97331 (541) 737-1772 zhangl3@science@oregonstate.edu Last Updated: 02/20/2011	Services offered: Proteomics
MS Core lab-Proteomics and Metabolomics

Bioinformatics Core University of Vermont 200A Farrell Hall 210 Colchester Ave Burlington, VT 05405 (802) 656-9798 James.Vincent@uvm.edu View Website Primary Contact: James Vincent Last Updated: 02/17/2011	Services offered: Computational - Bioinformatics Associations: NERTRN INBRE
The goal of the Bioinformatics Core is to provide support for the design and analysis of molecular biological experiments. To accomplish this goal we meet with investigators to identify consensus needs and then develop "pipelines" consisting of support services that efficiently satisfy these needs. Additionally the Bioinformatics Core is the curator of the Core Facilities Database you are currently viewing

Genomic Technologies Facility Iowa State University 2025 Roy J. Carver Co-Laboratory Ames, IA 50011-3650 (515) 294-7491 gtf@iastate.edu View Website Primary Contact: Mitzi Wilkening (515) 294-7491 gtfstaff@iastate.edu Last Updated: 02/11/2011	Services offered: Data Analysis Genomics Genotyping Microarray Nucleic Acid Extraction Real-time qPCR
The Genomic Technologies Facility (GTF) provides expertise and equipment to conduct high-throughput research. Our services include: * Sequenom MassARRAY® iPlex® genotyping * Sequenom MassARRAY® iPlex® allelotyping * Bulk Segregation Analysis maize mutant mapping * Single SNP genotyping * NimbleGen Sequence Capture * NimbleGen Comparative Genomic Hybridizations * Real-Time Thermocyclers for qPCR For details on these services, please visit: http://www.plantgenomics.iastate.edu/gtf/services/ ---- Sequenom Mass Spectrometer Sequenom Nanodispenser NimbleGen Hybridization Stations Roche Lightcycler 480 Stratagene Mx4000

MSU Functional Genomics Core Facility Montana State University Dept of Microbiology 109 Lewis Hall Bozeman, MT 59717 (406) 994-5666 kmcinnerney@montana.edu View Website Primary Contact: Kate McInnerney (406) 994-5666 kmcinnerney@montana.edu Last Updated: 02/10/2011	Services offered: Data Analysis Genomics Microarray Real-time qPCR RNA analysis RNA Integrity Associations: ABRF EPSCoR INBRE MWACD NICL
The Functional Genomics Core Facility provides instrumentation and support for academic investigators, primarily in Montana and the Rocky Mountain west. The core provides instrumentation and support for nucleic acid QC, Affymetrix, Nimblegen and Combimatrix (CustomArrays) array processing, and qPCR. We can also provide assistance with microarray analysis using a variety of open-source software. ---- RNA QC: NanoDrop, TBS-380, Agilent Bioanalyzer Affy array: GCOS 7 scanner, Fluidics Station 450 Nimblegen array: Nimblegen Hyb4, GenePix 4000B Rotating hyb ovens for spotted arrays (or Combimatrix arrays). qPCR: Corbett RotorGene 6000, Qiagen RotorGeneQ, Corbett CAS-1200

Genomics Core Facility University of Virginia Department of Biology P.O. Box 400328 Charlottesville, VA 22904-4328 (434) 243-9657 jpc7b@virginia.edu View Website Primary Contact: John Chuckalovcak (434) 243-9657 jpc7b@virginia.edu Last Updated: 01/28/2011	Services offered: Assays and Measurements Data Analysis DNA Analysis Genomics Genotyping Nucleic Acid Extraction Real-time qPCR RNA analysis RNA Integrity Sequencing - Capillary Sequencing Sequencing - DNA Sequencing Sequencing - Next-Gen Sequencing Sequencing - Pyrosequencing
The University of Virgina's Genomics Core Facility is a burgeoning, fully equipped laboratory that strives to provide high-quality 454 GS FLX genome sequencing service at extremely competitive prices. We welcome customers from all areas of the life science research community. ---- 454 GS FLX & PSSC Analysis Cluster, Agilent BioAnalyzer 2100, Beckman-Coulter Z1 Particle Counter, Qiagen Tissue Lyser, Bio-Rad DNA Engine Tetrad 2, TBS-380 Fluorometer, Qubit Fluorometer, NanoDrop ND-1000, ABI 3130xl Capillary DNA Sequencer

IBNAM Peptide Synthesis Core Facility Northwestern University 303 E. Superior St. Lurie 11-257 Chicago, IL 60611 (312) 503-6725 x-yue@northwestern.edu View Website Primary Contact: Xuan Yue Last Updated: 01/10/2011	Services offered: Mass Spectrometry Peptide Synthesis
The IBNAM Chemistry Core Facility is a dedicated peptide synthesis facility for IBNAM members, NU researchers, and other local academic and commercial labs. Our facility provides customers with high quality peptides synthesized at 0.1-2mmol scales and purified by reverse-phase HPLC. We can also carry out side chain or terminal modifications such as the addition of fluorescent or biotin labels according to the customers’ research applications. In addition, we utilize the leading-edge Agilent 6520 Q-TOF LCMS system to develop and deliver a wide range of mass spectrometry services. ---- Peptide Synthesis CEM Liberty 12-Channel Automated Microwave Peptide Synthesizer CS Bio CS136XT peptide Synthesizer Purification Varian ProStar210 Preparative HPLC Agilent 1200 Preparative HPLC Mass Spectrometry Agilent 6520 Q-TOF LCMS

GeoSpatial Resource Dartmouth Medical School Norris Cotton Cancer Center One Medical Center Drive Lebanon, NH 03756 (603) 653-6023 Heather.Carlos@Dartmouth.edu View Website Primary Contact: Heather Carlos (603) 653-6023 Heather.Carlos@Dartmouth.edu Last Updated: 01/07/2011	Services offered: Data Analysis GeoSpatial Analyis Associations: Cancer Center
The primary mission of the GeoSpatial Resource is to support the implementation of geospatial analysis for cancer research. Our goal is to provide expert consultation and collaboration for research projects of in behavior, epidemiology, and health services research. The GeoSpatial Resource also strives to educate members of the community in different aspects of geospatial analysis by providing courses through TDI and at Dartmouth College. We can provide assistance with acquisition of spatial data, project design, spatial analysis and mapping.

Mass Spectrometry Laboratory University of Texas Health Science Center at San Antonio San Antonio, TX 78229 (210) 567-4043 weintraub@uthscsa.edu View Website Primary Contact: Susan T. Weintraub (210) 567- weintraub@uthscsa.edu Last Updated: 01/06/2011	Services offered: 2D-PAGE Mass Spectrometry Associations: ABRF Cancer Center
Mass Spectrometry for small molecule and proteomic analyses. Research services include: molecular mass determination, protein identification (in gel or solution digestion), identify individual macromolecules in complex mixtures, Sites of post-translational modification, and high-sensitivity, multiplexed, absolute quantification of peptides.

Center for Macromolecular Interactions University of Texas Health Science Center at San Antonio San Antonio, TX 78229 (210) 567-3332 demeler@biochem.uthscsa.edu View Website Primary Contact: Virgil Schirf (210) 567-6590 schirfv@biochem.uthscsa.edu Last Updated: 01/05/2011	Services offered: Biochemical Analysis Light Scattering Surface Plasma Resonance (SPR) Analytical Ultracentrifugation Associations: ABRF Cancer Center
CMMI provides for the complete characterization of a macromolecular interaction in solution. This includes a description of the kinetics, thermodynamics and assembly state of the interaction. An unusal strength of our facility is that access to all complementary, biophysical technologies are available in a single facility.

Biomolecular NMR Core University of Texas Health Science Center at San Antonio San Antonio, TX 78229 (210) 567-8780 ahinck@biochem.uthscsa.edu View Website Primary Contact: Andrew Hinck (210) 567-8780 ahinck@biochem.uthscsa.edu Last Updated: 01/05/2011	Services offered: Nuclear Magnetic Resonance (NMR) Associations: ABRF Cancer Center CTSA
Biomolecular NMR Spectroscopy offers state-of-the-art high field NMR instrumentation for structural studies of biological macromolecules. The instrumentation presently available includes four-channel Bruker Avance 500, 600, and 700 MHz NMR spectrometers. The facility also has available an ultrahigh sensitivity 5mm 1H-13C-15N triple-resonance cold probe for the 600 MHz spectrometer. The resources of the facility can be accessed either on a fee-for-service or on a collaborative use basis. The types of analyses conducted on a fee-for-service basis include acquisition and analysis of the required spectra for elucidation of small molecule structures (includes synthetic molecules, natural products, cofactors, lipids, and short peptides (30 amino acids or less)). ---- 500MHz Spectrometer 600MHz Spectrometer with an ultrahigh sensitivity 5mm 1H-13C-15N triple-resonance cold probe 700MHz Spectrometer

Protein Expression The Wistar Institute 3601 Spruce Street Philadelphia, PA 19104 (215) 898-3814 dschultz@wistar.org View Website Primary Contact: Dave Schultz (215) 495-6885 dschultz@wistar.org Last Updated: 12/30/2010	Services offered: Protein Extraction/Purification Viral Vectors Virology Western Blot Protein Expression Associations: Cancer Center
The Protein Expression and Libraries Facility at The Wistar Institute provides state-of-the art services to produce recombinant proteins in prokaryotic and eukaryotic expression systems for the scientific community. The laboratory has over 20 years of experience in producing recombinant proteins via these technologies. The major services provided include: 1. Viral vector production (i.e. baculovirus and retrovirus), 2. Analytical and preparative scale expression of nascent or epitope-tagged recombinant proteins, 3. One- and two-step affinity tag protein purification. The goals of the facility are accomplished by a centralized laboratory with dedicated laboratory staff, which allows for high-throughput, economy of scale, virus production and protein expression services, including quality assurance and control procedures to ensure efficient, consistent production and purification of recombinant proteins and viral vectors. Many recombinant proteins produced by the facility have been used for crystallization efforts, enzymology, structure-function relationships between protein-protein, protein-nucleic-acid, and protein-small molecule interactions, custom antibody production, experimental cancer vaccines, and development of miniaturized assays for small molecule screening.

BioImaging Rensselaer Polytechnic Institute 1650 15th Street Troy, NY 12180 (518) 276-4286 mccals@rpi.edu View Website Primary Contact: Marimar Lopez (518) 276-4416 lopezm4@rpi.edu Last Updated: 12/13/2010	Services offered: Clinical Imaging - Small Animal (X-ray, Ultrasound, microCAT, MRI) Magnetic Resonance Imaging (MRI)
Imaging instrumentation: 7T/13cm horizontal and 14T/9cm vertical bore MRI Scanners, Bruker Ultraflex III MALDI TOF/TOF with imaging capabilities and a small animal Scanco Medical Viva CT40 micro-CT scanner ---- Our webpage construction is in progress. Please contact us for consultation and rates.

NMR Core Rensselaer Polytechnic Institute 1607 15th street Troy, NY 12180 (518) 276-2856 mccals@rpi.edu View Website Primary Contact: Marimar Lopez (518) 276-4416 lopezm4@rpi.edu Last Updated: 12/13/2010	Services offered: Nuclear Magnetic Resonance (NMR)
Active projects also include investigating structural aspects of Alzheimer's disease, structure-function analysis of protein glycosylation, mechanism of an anthrax inhibitor, and the structural basis for protein recognition of heparin. ---- if interested contact Scott A. McCallum, Ph.D. Director, NMR Core Facility Center for Biotechnology and Interdisciplinary Studies, Room 1143 Rensselaer Polytechnic Institute 110 8th Street Troy, NY 12180-3590 E-mail: mccals@rpi.edu ---- 800 MHz NMR Spectrometer with Cryoprobe 600 MHz NMR Spectrometer with Cryoprobe 600 MHz Wide-bore NMR Spectrometer with Solid-state and Microimaging A wide variety of solid-state and solution-state NMR probes are available.

UAlbany Proteomics Facility University at Albany, SUNY Center For Functional Genomics One Discovery Drive, 342H Rensselaer, NY 12144 (518) 591-7214 linq@albany.edu View Website Primary Contact: Qishan Lin Last Updated: 12/09/2010	Services offered: 2D-PAGE Assays and Measurements Biochemical Analysis Gel Chromatography Mass Spectrometry Protein Extraction/Purification Proteomics SDS-PAGE Sequencing - Protein Sequencing Western Blot Associations: ABRF
The Proteomics Core Facility combines in-depth expertise with state-of-the-art facilities for the large-scale characterization of proteins expressed in health and disease. Proteomics involves the identification of proteins in the body and the determination of their role in such activities as transmitting disease. The goal is to identify protein structures; interactions and pathways so that new disease markers and drug targets can be identified that will help create new products to prevent, diagnose and treat diseases. Our scientific team has experience in all facets of proteomics analysis including sample processing for high-resolution 2D gel electrophoresis, multidimensional chromatography, isotopic labeling mass spectrometry analysis by ESI/MS/MS, sequence analysis, and bioinformatics. These results, in conjunction with genomic database comparisons, enable us to describe the potential role of novel proteins identified through our proteomics discovery platform. The proteomics and mass spectrometry services at CFG are accessible to any academic investigators from any institution as well as clients from industry. We offer: Liquid chromatography Protein and peptide purification 1D and 2D gel electrophoresis Gel imaging and analysis Sample processing (isotope labeling, enzymatic digestion and fractionation) MALDI-TOF peptide mass fingerprint (PMF) Protein identification (Protein ID) Phosphopeptide mapping (TiO2IMAC, LC-MS/MS approaches) Protein profiling though MudPIT, ITRAQ or SILAC Biomarker discovery Metabolite ID and quantitation De novo peptide sequencing Amino acid analysis We work closely with our clients in a professional and confidential

Sequencing and Genotyping - Allen Laboratory University of Delaware Delaware Biotechnology Institute room 246 15 Innovation Way Newark, DE 19711 (302) 831-0823 brucek@udel.edu View Website Primary Contact: Bruce Kingham brucek@udel.edu Last Updated: 12/08/2010	Services offered: DNA Analysis Genotyping Nucleic Acid Extraction Real-time qPCR RNA Integrity Sequencing - Capillary Sequencing Sequencing - DNA Sequencing Sequencing - Next-Gen Sequencing Associations: ABRF EPSCoR INBRE
The DNA Sequencing & Genotyping Center provides state of the art genetic analysis for University of Delaware research groups and outside users on a fee-for-service basis. The Applied Biosystems 3130XL Genetic Analyzer housed in the center is a state-of-the-art capillary-based instrument capable of moderate- to high-throughput DNA sequencing and fragment analysis (up to 656 samples/day). The center is currently operating the Genome Analyzer II from Illumina. This platform is massively parallel genome sequencer capable of 6 GB of output for a single run.

Reproductive Sciences The Jackson Laboratory 600 Main Street Bar Harbor, ME 04609 (207) 288-6000 rob.taft@jax.org View Website Primary Contact: Rob Taft (207) 288-6727 john.kulik@jax.org Last Updated: 12/02/2010	Services offered: Animal Husbandry Assisted Reproductive Technologies (Rodent IVF, ICSI) BioBanking Cell Culture Embryo Cryopreservation & Recovery Services Stem Cell Procurement Tissue Culture Transgenics Gamete Cryopreservation Associations: NERTRN Cancer Center INBRE
Reproductive Sciences is composed of Importation, Cryopreservation, Cell Biology and Microinjection groups. These groups provide a variety of services including creation of genetically modified mice, cryopreservation, recovery of cryopreserved mouse strains, rapid expansion of lines using in vitro fertilization, rederivation of imported mouse lines and maintenance of a repository of publicly distributed cryopreserved embryos, sperm and ovaries. In addition to providing these services, the Reproductive Sciences group maintains an active research program focused on the development, refinement and implementation of assisted reproductive technologies that can be used to manage strains more effectively and an education program designed to facilitate the transfer of these technologies to the scientific community.

Analytical Imaging Facility Albert Einstein College of Medicine 1300 Morris Park Avenue Jack & Pearl Resnick Campus Bronx, NY 10461 (718) 430-3547 frank.macaluso@einstein.yu.edu View Website Primary Contact: Frank Macaluso (718) 430-3547 macaluso@aecom.yu.edu Last Updated: 11/30/2010	Services offered: Cell Imaging Confocal Microscopy Cryo-Electron Microscopy Data Analysis Electron Microscopy Immunohistochemistry Microscopy Molecular Imaging Optical Imaging Associations: Cancer Center
The Analytical Imaging Facility provides a comprehensive light and electron microscope imaging facility dedicated to bringing state of the art methods in modern imaging to biomedical scientists with all levels of expertise. The AIF staff has been cross-trained to offer a seamless transition from classical histology, to high resolution light microscope imaging in 3D, to state of the art electron microscopy. This unified approach facilitates the efficient and appropriate complementary use of these methods in research. For the infrequent user, the AIF provides a completely assisted technical support service. For the trained microscopist, the AIF is an available equipment resource. A significant effort is devoted to training investigators who require microscopy techniques to advance their projects.

South Carolina College of Pharmacy Microarray Core Facility University of South Carolina at Columbia Dept. of Pharmaceutical and Biomedical Sciences, University of South Carolina 715 Sumter Street, CLS 617 Columbia, SC 29208 (803) 777-6682 altomared@sccp.sc.edu View Website Primary Contact: Dr. Kim E Creek (803) 777-0952 creekk@sccp.sc.edu Last Updated: 11/29/2010	Services offered: Computational - Bioinformatics Data Analysis Genomics Microarray Outreach RNA analysis RNA Integrity Associations: INBRE
Services Provided: The USC Microarray Core Facility (formerly the SCCC DNA Microarray Facility) can produce Custom Arrays, support Array Design, conduct RNA quality control, amplification and labeling; conduct array hybridization, scanning and data analysis, and assist customers in all aspects of the design and performance of microarray experiments, using Agilent (pre-made and custom) and spotted arrays. In addition, the facility provides support for quantitation and quality assessment of DNA, RNA and protein on an Agilent 2100 Bioanalyzer. Custom Arrays The facility is equipped to provide custom microarrays for academic and industrial clients. So far we have successfully spotted cDNAs, 50 to 70 base pair oligonucleotides and 18 to 22 base pair short primers. In addition to nucleic acids, the Biochip Arrayer can spot antibodies, proteins, peptides and bacterial cells as well. We produce arrays of the highest quality but at a low density (up to about 2,000 spots per slide). Array Design We assist all clients in designing the appropriate microarray for your needs. We take the guesswork out of array alignment and maintain a high level of spatial precision throughout the printing run. Specification with regard to array design and spot placement is tightly monitored. This attention to detail results in saved scanner time and fewer auto format failures. Hybridization We provide extensive on site training and support for all HybStation, Maui, and Agilent’s Hybridization Oven users. Scanning and Data Analysis Support is provided for spotted microarray scanning and for data analysis with a variety of software packages, including GenSifter. Analysis of RNA, DNA, Proteins and Cells Quantity and quality of your RNA, DNA, proteins and cells are determined with the help of an Agilent 2100 Bioanalyzer. ---- We have helped clients with all aspects of their microarray experiments, including devising specific methods for the extraction of high-quality RNA from difficult samples, such as for example the microcrostacean Daphnia (D. Pulex) and Pap test material. ---- The Facility includes: (1) a Packard Bioscience Biochip Arrayer. Based on Ink jet technology the arrayer has 4 piezoelectric capillary tips that achieve extremely accurate non-contact dispensing. When in operation the glass capillaries of PiezoTip dispensers cruise over a glass slide or other substrate at a height of 500 microns, dispensing droplets of 350 picoliters to a target with a X/Y positional accuracy of 10 microns. The Biochip Arrayer is ideal for the production of extremely high quality arrays of up to about 2,000 spots/slide, custom-made to satisfy the requirements of individual investigators. The arrayer also has enormous flexibility in terms of substrate and spotted product, being able to spot on a variety of materials, and not only nucleic acids but also proteins, peptides, and even bacterial cells; (2) an automatic hybridization system in the form of a 12 position GeneTac HybStation. Up to six protocols can be run simultaneously, which allows for hybridization with uniform, reproducible results and minimal handling of solutions and slides; (3) a hybridization oven and rotator, which are optimized for the hybridization of high-density arrays from Agilent Technologies and a Maui Hybridization System; (4) a Perkin Elmer confocal laser scanner Scan Array Express HT is available for microarray scanning using up to 5 lasers which allow for the detection and quantification of signal from virtually all commercially available fluorochromes including the commonly used Cy3 and Cy5; (5) an Agilent Technologies 2100 Bioanalyzer that allows for the rapid assessment of RNA quality (software available with this instrument assigns an RNA Integrity Number (RIN) to the samples, based on various parameters of RNA integrity; and (6) a powerful 8-tip automated liquid handling system – the Perkin Elmer Multiprobe II - to ensure accuracy and reliability in the handling and arraying of oligonuclotides and cDNAs prior to spotting.

Laboratory for Molecular Biology and Cytometry Research University of Oklahoma Health Sciences Center 975 NE 10th Street BRC1106 Oklahoma City, OK 73104 (405) 271-2337 microgen_support@ouhsc.edu View Website Primary Contact: Dr. Allison Gillaspy (405) 271-1201 x 1 allison-gillaspy@ouhsc.edu Last Updated: 11/23/2010	Services offered: Cell Imaging Cell Sorting Computational - Bioinformatics DNA Analysis Flow Cytometric Analysis Genomics Mass Spectrometry Microarray Microscopy Nucleic Acid Extraction Proteomics Real-time qPCR RNA analysis RNA Integrity Sequencing - Capillary Sequencing Sequencing - DNA Sequencing Sequencing - Next-Gen Sequencing Sequencing - Protein Sequencing Surface Plasma Resonance (SPR) small molecule interactions using SPR Associations: COBRE ABRF INBRE NICL
The OUHSC Laboratory for Genomics and Bioinformatics is an academic core facility offering a wide range of services involving DNA, protein, and whole cell analysis. Services include DNA sequencing (custom and genome-scale projects) via both the Applied Biosystems SOLiD Next generation sequencing platform and 3730xl capillary sequencers. Other services include high-throughput plasmid prepping, protein separation using the Beckman PF2D system, mass spectrometry (analytical and preparative), analysis of complex protein mixtures by iTRAQ, cell sorting, flow cytometry, Luminex, and imaging. All services are offered to OUHSC researchers and outside users. ---- SOLiD Next Generation Sequencer, QStar Mass spectrometer, Brueker mass spec, Agilent Bioanalyzer, 3730xl capillary sequencer, NX Biomek, 7500 Fast Real Time system, FACSCalibur Flow cytometer, Nikon TE2000-E, Leica TCS_NT and Leica SP2_NP confocal microscopes, BioPlex 200, Influx Cell sorter, BiaCore T100, Voyager DE Pro (MALDI-TOF)

Protein Production Lab, Center of Structural Biology Medical University of South Carolina 526 BSB, 173 Ashley ave Charleston, SC 29425 (843) 792-6529 fedarov@musc.edu View Website Primary Contact: Dzmitry Fedarovich (843) 792-6529 fedarov@musc.edu Last Updated: 11/22/2010	Services offered: Cell Culture Gel Chromatography Light Scattering Molecular Construct Services Nucleic Acid Extraction Protein Extraction/Purification SDS-PAGE Western Blot CD, DLS Associations: COBRE
Lab produces purified proteins on a large scale to support research activities at MUSC and outside customers. The lab also contains equipment for biophysical characterization of proteins, including circular dichroism and dynamic light scattering ---- BioFlo 110 Fermentor, C45KC shaker/incub. (New Brunswick); AKTA-FPLC, PhastSystem (Amersham); CD spec M400 (AVIV); DLS (Precision Detectors); ultracentrifuge (Beckman); VP-ITC microcalorimeter(MicroCal); etc.

Transgenic and Targeted Mutagenesis Laboratory Northwestern University Olson Pavilion, 8402 710 North Fairbanks Court Chicago, IL 60611 (312) 503-0773 TTML@northwestern.edu View Website Primary Contact: Lynn Doglio (312) 503-0088 x 3-0088 l-doglio@northwestern.edu Last Updated: 11/19/2010	Services offered: Embryo Cryopreservation & Recovery Services Genotyping Stem Cell Procurement Tissue Culture Transgenics Sperm Cryopreservation and Recovery Services, IVF services, Gene Targeting services, ES cell Microinjection Services Associations: Cancer Center MWACD
The Northwestern University Transgenic and Targeted Mutagenesis Laboratory (TTML) is a shared resource that provides a broad range of services to NU investigators, including generation of transgenic mice, gene targeting of embryonic stem (ES) cells, ES cell microinjection into blastocysts, mouse germplasm cryopreservation, recovery of cryopreserved mouse lines, and rederivation of pathogen free mouse strains. ---- ES Cell Microinjection into Blastocysts, Embryo Cryopreservation, Embryo Cryorecovery, Gene Targeting/ES Cell Culture, Generation of Transgenic Mice/Embryos, Rederivation of Pathogen Free Mouse Strains, Sperm Cryopreservation, Sperm Cryorecovery

Next Gen Sequencing and Analysis Center National Center for Genome Resources (NCGR) 2935 Rodeo Park Drive East Santa Fe, NM 87505 (505) 995-4449 fds@ncgr.org View Website Primary Contact: Faye Schilkey (505) 995-4449 fds@ncgr.org Last Updated: 11/17/2010	Services offered: Computational - Bioinformatics Data Analysis DNA Analysis Genomics Genotyping RNA analysis Sequencing - Capillary Sequencing Sequencing - DNA Sequencing Sequencing - Next-Gen Sequencing mRNA seq, small RNA seq, ChIP seq, DGE, JMP Genomics Associations: INBRE NICL
Genome Sequencing Center ------------------------- SEQUENCING o Illumina Certified Service Provider (1st in North America) o Illumina GAIIx & HiSeq2000, SOLiD, PacBio RS o Whole Genome/Transcriptome Shotgun, mRNA Seq, ChIP-seq, small RNA o Singleton and mate-paired reads TARGET SELECTION o Enrich subsets of genes and other selected regions using NimbleGen, Agilent or Raindance technologies INFORMATICS o Genome/Transcriptome Analysis o Alpheus® cloud-based analysis system for Next–Gen sequencing data (http://alpheus.ncgr.org/) o Nucleotide variant, in/dels, premature stop codons, splice isoforms, genomic rearrangements, and expression detection o JMP-Genomics expertise and interface for downstream statistical analysis o Custom Assembly/Annotation services GENOTYPING o Illumina-based genotyping assay for human, plants and animals Sequencing infrastructure: Cluster stations for flow cell construction, and Agilent bioanalyzer, NanoDrop, PicoGreen devices for sequencing library quantification QA/QC. The wet lab has a full compliment of molecular laboratory equipment (e.g. ultra-low , -20 and 4 oC temperature freezers, centrifuges, agarose gel electrophoresis and gel documentation equipment) to support up to four workstations/researchers. IT Infrastructure: Data Storage (file servers) - Four Sun X4500 - 24 TB (96TB total) - One Sun X4540 48TB - Isilon cluster 144TB Image processing/Basecalling - Two Sun X4150 and and one x4170 – Total: 6 x - 4-core processors, 24 GB RAM, 12 x 146GB Hard drives Variant detection - 10 Sun Blade 6000 chassis with 100 x 6220 Blades (600 Cores, 2TB of RAM and 58.4 TB disk) DB storage - Two sun 4150, two 4450 DB Servers, 6140 SAN with 70 TB for DB disk space, 4 Gb/s Switch fabric ---- Alpheus® Next Generation Sequence Data Analysis: Analyzes high-throughput, low-cost next generation sequencing data (Illumina-Solexa, Roche-454, ABI-SOLiD) with characteristically short reads is problematic for conventional assembly and analysis pipelines. The Alpheus software system identifies variants (i.e. SNPs or indels) in these short reads and filters them based on consistency, allele frequency, quality, coverage, and variant type to reduce false positives. Alpheus also determines gene expression, comparing values across groups of samples. Alpheus features automated data handling, a computational analysis pipeline, a relational database and a web-based query and visualization interface allowing researchers secure access via any web browser.

Human Embryonic Stem Cell Core Facility UMass Medical School 55 Lake Ave North S3-308 Worcester, MA 01655 (508) 856-3269 stemcell.corefacility@umassmed.edu View Website Primary Contact: Gary Stein, Jane Lian and Matthew Mandeville (508) 856-3269 Last Updated: 11/17/2010	Services offered: Cell Culture Karyotyping Associations: NERTRN
The HESC Core facility will provide, to UMASS investigators on all campuses, undifferentiated stem cells; two NIH approved cell lines, H1 (male) and H9 (female), on mouse feeder layers or feeder cell free. Our support services include: •Quality controlled undifferentiated stem cells plated on inactivated MEF feeder layers or Matrigel •Protocols for monitoring HESCs during studies •Advice on experimental design ---- Quality control includes routine mycoplasma testing.

Confocal and Conventional Microscopy Core University of Rochester Medical Center 601 Elmwood Ave Rochester, NY 14642 (585) 275-1317 Linda_Callahan@urmc.rochester.edu View Website Primary Contact: Linda Callahan (585) 275-1317 Linda_Callahan@urmc.rochester.edu Last Updated: 11/17/2010	Services offered: Cell Imaging Confocal Microscopy Microscopy Optical Imaging Image Analysis Associations: ABRF
Mission Statement: The URMC Confocal and Conventional Microscopy Core strives to provide researchers the ability to obtain high quality imaging data using state-of-the-art microscopy instruments. The Core also aims to be one of the information hubs for UR resources centering around histological processing, imaging, and image processing and to serve as a conduit for communication between imaging researchers on campus. Core Overview: The Confocal and Conventional Microscopy Core provides Medical Center researchers access to high-end confocal microscopy as well as more conventional brightfield and fluorescence microscopy. The Core provides assistance with all imaging procedures as needed by the investigators. Aid in customizing staining protocols to optimize obtainment of high quality data using Core instruments is also provided. The CCMC provides researchers the ability and expertise to obtain high quality imaging-based data from tissue, culture, and materials-based research studies. For further information, contact Linda_Callahan@urmc.rochester.edu Best contact is via email. ---- CCMC Instruments: 1. Olympus FV1000 confocal microscope with SIM scanner (for simultaneous bleaching and imaging) 2. Vanox AH-2 conventional microscope 3. Large Specimen Instrumentation (for imaging of gross anatomical slices and large histological specimens) 4. Image Analysis using StereoInvestigator (Optical Fractionator and Space Balls), and Image Pro Plus. Current versions, contract supported.

NUFAB Cleanroom Northwestern University 2145 Sheridan Road EG20-NG32 Technological Institute Evanston, IL 60208 (847) 467-6201 nufab@northwestern.edu View Website Primary Contact: Nasir Basit - x 7-6201 n-basit@northwestern.edu Last Updated: 11/17/2010	Services offered: Characterization, Deposition, Etching - Ashing, Photolithography Associations: MWACD
NUFAB is an advanced micro/nano fabrication research facility at Northwestern University. It provides resources for research in MEMs/NEMs, nano-bio, nano/microelectronics, and other related and interdisciplinary areas to Northwestern University community and outside researchers. It currently resides in EG20-NG32 Technological Institute and has all new research equipment. Please check the equipment list on this site. In 2012, it will relocate to a new clean room facility at FG infill that is under construction ---- Aligner - Suss MABA6 Mask Aligner, Convection Ovens - BlueM DCC-146C, Deep Reactive Ion Etcher, DRIE - STS LpX Pegasus, Develop Hood, Digital Hotplates, Electrical Test Station, Microscope - Nikon LV150, Parylene Coater - SCS Labcoter2 Parylene Deposition System, Plasma Enhanced Chemical Vapor Deposition, PECVD - STS LpX CVD, Reactive Ion Etcher, RIE - Samco RIE-10NR, Reflectometer - Filmetrics F20, Spinner - Brewer Science Cee Model 200 Spin Coater, Sputtering - AJA Orion Sputter, Stylus Profilometer - Veeco Dektak-8, Thermal Evaporator - Denton Vacuum Explorer 14

Plant Tissue Culture and Transformation Donald Danforth Plant Science Center 975 North Warson Road St. Louis, MO 63132 (314) 587-1816 klutke@danforthcenter.org View Website Primary Contact: Kevin Lutke (314) 587-1634 x 3145871634 klutke@danforthcenter.org Last Updated: 11/17/2010	Services offered: Cell Culture Outreach Tissue Culture Transgenics
The Plant Tissue Culture and Transformation Facility operates both as a full service facility delivering transgenics and cell cultures to researchers and a self service facility providing high quality space for researchers to use for their own specific project needs. The facility currently consists of a 880 sq. ft. single large lab along with a 225 sq ft annex for transformation and tissue culture work, over 880 sq.ft of culture growth space, and a dedicated media kitchen for media preparation. ---- Transformation services include: soybean, maize, rice, tomato, tobacco, Indian Mustard, and Arabidopsis ---- The facility contains almost all the essential equipment necessary for plant cell and tissue culture and transformation. •Fourteen laminar flow tissue culture hoods •Refrigerated Eppendorf centrifuge •Bench top Eppendorf microfuge •Shimadzu spectrophotometer •Seven Olympus dissecting microscopes (one with photo capabilities) •Inverted microscope •Incubators (both wet and dry) •Culture incubator/shaker •Two floor model temperature controlled shakers •BioRad GenePulser electroporator •BioRad PDS-1000 gene delivery system •A hand-held BioRad Helios system

Hunter College Digital Bio-Imaging Facility Hunter College, CUNY Room 826 HN 695 Park Ave New York, NY 10065 (212) 650-3872 williams@genectr.hunter.cuny.edu View Website Primary Contact: Lloyd Williams (212) 650-3872 williams@genectr.hunter.cuny.edu Last Updated: 11/17/2010	Services offered: Cell Imaging Confocal Microscopy Electron Microscopy Microscopy Optical Imaging Phosphor Imaging Associations: RCMI CTSA ABRF
This facility currently provides optical and electron microscopy services. The facility is equipped with two spinning disk confocal microscopes, a Leica TCS SP2, and a Perkin Elmer Ultra VIEW ERS Spinning Disc confocal. In addition to confocal microscopy the facility is also equipped for bright field, phase contrast, modulation contrast and epi fluorescence microscopy. The facility has two separate wide field image analysis stations. A Nikon Optiphot microscope equipped with a color video system and Compix imaging software, and a Nikon Eclipse TE200 inverted scope. This inverted scope in specially configured to perform calcium ratio imaging is equipped with Molecular Devices Meta Fluor software The facility has recently expanded with the acquisition of a JEOL JEM-100C/CX Transmission Electron Microscope. The facility also is equipped with Typhoon 9410 scanner for the quantization and localization of sub cellular fluorescent and radioactive molecules. In addition a Densitometer allows for the analysis of 1 and 2 D gels, Southern, northern and western blots and RFPL gels. The facility also has recently acquired an Odyssey Infrared Imager. ---- 1.Laser scanning (Leica) and spinning disk (PerkinElmer) confocal microscopes. 2. Calcium Ratio & Micro Injection Inverted Scope (equipped with Metafluor software). 3. Molecular Dynamics Densitometer. 4. Wide field microscopes: bright field and fluorescence. 5. Gemini EM microplate Fluorescence spectrophotometer. 6. Image analysis (Metamorph and Volocity). 7. Molecular Dynamics Typhoon 9410 for Chemiluminescence and Autoradiography. 8 Dissecting scope with Video Editing Station. 9. JEOL 100C/CX Transmission Electron Microscope. 10. PowerWave Microplate Spectrophotometer. 11. Odyssey Infrared Imager 12. GloMax 96 Microplate Luminometer

The Jackson Laboratory - Computational Sciences The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 (207) 288-6339 chuck.donnelly@jax.org View Website Primary Contact: Chuck Donnelly (207) 288-6339 chuck.donnelly@jax.org Last Updated: 11/16/2010	Services offered: Computational - Bioinformatics Computational - Biostatistics Computational - High Performance Computing Data Analysis DNA Analysis Genomics RNA analysis Scientific Software Development Associations: NERTRN ABRF Cancer Center
The Computational Sciences core is an interdisciplinary group of bioinformatics software engineers, biologists, and mathematicians providing statistical consulting, data analysis, method development, algorithm design, software engineering, LIMS development, high performance computing, regional compute grid development, data resources, analysis tools and tool integration, and experimental design services to biomedical research investigators at The Jackson Laboratory. The CS core also manages the TJL liaison office to the NCI caBIG program. ---- Regional Compute Resources, Scientific Software Development High Performance Computing Laboratory Information Management Systems (development/support). Animal colony management software. ---- 500 Core HPC cluster

UW Biotech Center-Gene Expression Center University of Wisconsin-Madison 425 G Henry Mall Room 3450 Madison, WI 53706 (608) 265-3029 gecinfo@biotech.wisc.edu View Website Primary Contact: Sandra Spinter BonDurant (608) 265-3029 Last Updated: 11/16/2010	Services offered: Genomics Microarray Nucleic Acid Extraction Real-time qPCR RNA Integrity Sequencing - Next-Gen Sequencing Associations: MWACD ABRF
The Gene Expression Center is a fee-for-service facility that enables investigators to study an organism’s entire transcriptome in a single experiment by using state-of- the-art next generation sequencing and microarray technologies. If a researcher is interested in studying expressed genes, alternative splice variants, and novel transcripts present in the transcriptome, the GEC offers next generation sequencing applications, such as mRNA and Small RNA Seq, on Illumina’s® Genome AnalyzerII System. If the organism of interest is well studied, microarrays from Affymetrix and Roche NimbleGen can be used to study the changes in the organism’s transcriptome. Real time quantitative PCR service options are also available for validation of larger experiments or to interrogate a smaller specific set of genes.

SD BRIN Genomics Core Facility University of South Dakota 414 E Clark St Vermillion, SD 57069 (605) 677-5159 keyster@usd.edu View Website Primary Contact: Dr. Kathy Eyster (605) 677-5159 keyster@usd.edu Last Updated: 11/16/2010	Services offered: Data Analysis DNA Analysis Genomics Microarray Real-time qPCR Sequencing - DNA Sequencing Associations: INBRE
The Genomics Core Facility will continue to support DNA microarray analysis of differential gene expression, RNA and DNA analysis by the Agilent microfluidics chip reader, and real time PCR/RT-PCR. To maintain the relevance of the core, we believe that it is important to add new services to the core to enable examination of the functions of genes that have been shown to be differentially expressed by DNA microarray. To this end, the GCF is currently purchasing the Amaxa Nucleofector II transfection equipment. This technology allows transfection of DNA constructs, RNAi/siRNA/ shRNA, peptides, antibodies and other biomolecules into living eukaryotic cells. The Amaxa nucleofection system is optimized for transfection of cells that are otherwise difficult to transfect.

Flow Cytometry Core Facility University of Arkansas for Medical Sciences 4301 West Markham Little Rock, AR 72205 (501) 686-6927 aharris@uams.edu View Website Primary Contact: Andrea Harris (501) 686-6927 Last Updated: 11/16/2010	Services offered: Cell Sorting Data Analysis Flow Cytometric Analysis
The Flow Core facility offers several services including analysis by flow cytometry, cell sorting and cytokine analysis. The Facility has 3 instruments: FACS Calibur for up to 4-color analysis, DNA cell cycle, cell proliferation, etc, the FACS Aria for up to 9 color analysis as well as cell sorting, and the Bioplex for cytokine analysis.

Physiology Core University of Vermont Fletcher Allen Baird 793 Burlington, VT 05401 (802) 847-0433 nathan.kokinda@vtmednet.org View Website Primary Contact: Nathan Kokinda Last Updated: 11/15/2010	Services offered: Protocol Development/Clinical Trial Coordination Associations: NERTRN CTSA
The Physiology Core Laboratory at the GCRC offers an extensive array of services with emphasis on the measurement of body composition, bone mineral density, energy expenditure, exercise capacity, muscle strength testing, pulmonary function, functional capacity, and anthropometrics. A physiologist is available to discuss the detailed procedures involved in measures you may be considering for inclusion in your protocol and to assist with testing. Moderate to high risk patients/subjects undergo exercise testing under the supervision of a cardiologist at our GCRC satellite facility within the Cardiology Outpatient Facility at 62 Tilley Drive, So. Burlington, VT. Physiology Core Services and Equipment Physiology Core Equipment at the Satellite Facility

COVCD Analytical COre University of Kentucky, Lexington Room 248G BBSRB 741 South Limestone Lexingyon, KY 40536-0509 (859) 323-3749 a.j.morris@uky.edu View Website Primary Contact: Andrew Morris (859) 323-3749 a.j.morris@uky.edu Last Updated: 11/13/2010	Services offered: Mass Spectrometry Microarray Associations: COBRE
The Analytical Research Core provides state of the art methodologies for measurement of a broad range of biomolecules to the participating investigators. This goal has been accomplished by use of two complementary technologies: HPLC Tandem mass spectrometry and multiplex fluorescence (“Luminex”)-based assays. The core is directed by Dr. Andrew Morris, Professor of Internal Medicine and Molecular and Cellular Biochemistry. Manjula Sunkara, MS, Research Associate, is responsible for day to day operation of the core instrumentation. Ms Sunkara has a background in analytical chemistry with additional experience in biomedical mass spectrometry gained with a pharmaceutical research company. Ms Sunkara is supported entirely by the COBRE award. Operating expenses of the core are cost shared by the participating investigators and supported in part by funds provided by the Vice President for Research to facilitate use of the instrument by other non-COBRE affiliated researchers. Using funds provided by a previously awarded NCRR Shared instrumentation grant (1S10RR024598-01, Andrew J. Morris, PI) an interactive group of researchers at the University of Kentucky College of Medicine recently purchased an Applied Biosystems (ABI)/MDS Sciex 4000 Q-Trap hybrid triple quadrupole linear ion trap mass spectrometer. The instrument is equipped with an ABI Turbo V electrospray ionization ion (ESI) source and interfaced with a Shimadzu multi pump HPLC system equipped with a column oven and autosampler. A computer workstation running ABI Analyst Software is used for instrument control and data acquisition and processing. The instrument was put into service about 6 months ago and in the intervening time we have set up multiple reaction monitoring mode HPLC tandem mass spectrometry assays for analysis and quantitation of a broad and expanding range of lipids and lipid-related molecules which include glycero and sphingo phospholipids, fatty acids, eicosanoids, isoprenoids and sterols. Data obtained using this instrument have been published in the Journal of Biological Chemistry and Molecular Pharmacology with other reports and grant applications arising from experiments conducted using the instrument currently under review. More recently we have obtained a second AB Sciex 4000 Q-Trap mass spectrometer which is operated with an Agilent Quaternary HPLC system for small molecule quantitation. This instrument is also equipped with an AB Sciex flashquant vacuum MALDI ion source for high throughput multiwell format quantitation of small molecules and we have an interest in developing applications of this source for small molecule imaging in tissue specimens. The core also contains a Luminex (BioRad) 200 multiplex fluorescence analyzer. We provide assistance with calibration, experimental design and are working with the appropriate vendors to develop “panels” for collections of analytes that are tailored to the needs of the participating investigators. ---- AB Sciex 4000 Q-Trap hybrid triple quadrupole linear ion trap mass spectrometer Shimadzu HPLC system AB Sciex 4000 Q-Trap hybrid triple quadrupole linear ion trap mass spectrometer Agilent HPLC system Bioplex 200 (luminex) suspension array reader

Integrated Microscopy Facility Donald Danforth Plant Science Center Danforth Plant Science Center 975 North Warson Road St. Louis, MO 63132 (314) 587-1261 rhberg@danforthcenter.org View Website Primary Contact: Howard Berg (314) 587-1261 rhberg@danforthcenter.org Last Updated: 11/12/2010	Services offered: Cell Imaging Confocal Microscopy Cryo-Electron Microscopy Electron Microscopy Immunohistochemistry In Situ Hybridization Laser Capture Microdissection Microscopy Molecular Imaging Multiphoton Microscopy Optical Imaging
The facility offers instrumentation for live cell imaging and for thin section TEM. Light microscopes include confocal and multiphoton instruments. For TEM samples are prepared by high pressure freezing/freeze substitution. Also available are laser capture microdissection, fluorescence dissecting microscope, and a widefield microscope. We offer self and full services, and have extensive experience in imaging plant cells. ---- We offer full services for a number of sample processing protocols and for imaging samples on our instruments. ---- BalTec HPM 010 high pressure freezer; Leica AFS2 automated freeze substitution device; Leica UCT ultramicrotome; LEO 912AB energy filter transmission EM; Gatan 626 cryostage; Microm wax microtome; Zeiss PALM laser capture microscope with CryoJane tape transfer and cryostadt; Nikon SMT 1500 fluorescence dissection microscope; Nikon Eclipse 800 widefield microscope with dual Sutter filter wheels; Zeiss LSM 510 META confocal microscope plus Coherent Mira 900F Ti sapphire multiphoton laser; Nikon C1 confocal microscope; Bitplane Imaris image processing work station.

DNA Core Lab - Core Facility for Nucleic Acid Analysis University of Alaska PO Box 757000 909 N. Koyukuk Dr., 207 West Ridge Research Building Fairbanks, AK 99775 (907) 474-7336 fydna@uaf.edu View Website Primary Contact: Leif Vick (907) 474-7336 fydna@uaf.edu Last Updated: 11/12/2010	Services offered: DNA Analysis Genotyping Real-time qPCR Sequencing - DNA Sequencing Support Services - Shared Instrumentation Oversight & Maintenance Associations: INBRE EPSCoR
History: The Core Facility for Nucleic Acid Analysis was established at the Institute of Arctic Biology in 1995 with a grant from the National Science Foundation (Instrumentation and Instrument Development Program). It is a staffed facility intended to keep UAF at the "cutting edge" of nucleic acid analysis in evolutionary biology, molecular biology, and wildlife and fisheries management. UAF was recently the beneficiary of an NSF EPSCoR grant, an NIH BRIN (INBRE) grant, and an NIH COBRE grant, part of which was designated for new genomic instrumentation. Over the last few years the Core Facility has grown from having only one ABI 373 slab gel sequencer to having two capillary sequencers ( ABI 3100, ABI 3130xl) plus several other major pieces of instrumentation and various other supporting equipment. See Equipment listing on our website for more information on instruments. Services: The Core Facility provides nucleic acid sample analysis, and maintenance and support for molecular instruments (some of which may be too expensive for individual labs to own) for both UAF and outside users. The Core Lab facilitates learning by hosting training workshops and seminars for instrumentation and techniques to help students, faculty, and staff learn to use available equipment. The Core Lab also provides tours to middle and high school students/teachers, undergraduate students, graduate students, research staff, faculty candidates, and local or out of state visitors. The Core Lab has also been involved in several undergraduate/graduate classes offered by the Biology Department and the Chemistry Department. See Services and Fees on our website for more information on lab services. Users and Research: Of all facilities at UAF, the Core Facility may have the broadest interdisciplinary focus, since it attracts users from more than twenty PI labs from seven departments at UAF and UAA, the Alaska Sea Life Center, visiting students and scholars from Sweden, Russia, the Czech Republic, and others. See Past and Present Research for more research information.

UAMS DNA Sequencing Core Facility University of Arkansas for Medical Sciences 4301 W. Markham Slot 511 Little Rock, AR 72205 (501) 686-6927 giesallenj@uams.edu View Website Primary Contact: Allen Gies (501) 686-6927 giesallenj@uams.edu Last Updated: 11/12/2010	Services offered: Sequencing - Capillary Sequencing Sequencing - DNA Sequencing
The UAMS DNA Sequencing Facility provides DNA sequencing using an Applied Biosystems 3100 Genetic Analyzer. Turn around time is typically within two business days. Common vector primers are provided free of charge. New customers get two free reactions.

Molecular Research Core Facility Idaho State University 921 S. 8th Ave. Stop 8007 Gale Life Sciences Building Room 461 Pocatello, ID 83209-8007 (208) 282-4890 mrcf@isu.edu View Website Primary Contact: Erin O'Leary-Jepsen (208) 282-4890 oleaerin@isu.edu Last Updated: 11/12/2010	Services offered: Data Analysis DNA Analysis Flow Cytometric Analysis Genomics Genotyping Microarray Microscopy Nucleic Acid Extraction Optical Imaging Real-time qPCR RNA analysis Sequencing - Capillary Sequencing Sequencing - DNA Sequencing Support Services - Shared Instrumentation Oversight & Maintenance Western Blot Associations: NICL INBRE EPSCoR ABRF
The ISU Molecular Research Core Facility (MRCF), with its state-of-the-art instrumentation and ever-expanding array of services, provides ISU's molecular scientists with the resources necessary for successful and productive research pursuits in this rapidly growing field.

Rodent Metabolism Phenotyping Core Northwestern University Chicago, IL 60611 (312) 503-0217 rmpc@northwestern.edu View Website Primary Contact: Suhuan Liu - x 3-0217 rmpc@northwestern.edu Last Updated: 11/12/2010	Services offered: Assays and Measurements Nuclear Magnetic Resonance (NMR) Surgical Services Analytical, Sample Preparation Associations: MWACD
The RMPC facility is located in Center for Comparative Medicine in the basement barrier of the Lurie Buildings at Northwestern University Feinberg School of Medicine. The mission of the RMPC is to provide Northwestern University investigators with standardized, high quality metabolic phenotyping services to assist their characterization of rodent models of diabetes, obesity and metabolic disorders. ---- Nuclear magnetic Resonance (body composition-fat mass/lean mass/free fluid), Arginine stimulated insulin secretion, Glucose stimulated insulin secretion, IP glucose tolerance test, IP insulin tolerance test, Monitoring of blood glucose

Keck Interdisciplinary Surface Science Center - Keck II - part of NUANCE Northwestern University 2220 Campus Drive Cook Hall 1149 Evanston, IL 60208 (847) 491-5505 xchen@northwestern.edu View Website Primary Contact: Xinqi Chen (847) 491-5505 xchen@northwestern.edu Last Updated: 11/12/2010	Services offered: Mass Spectrometry Spectroscopy Image Analysis, Imaging Associations: MWACD
Keck-II hosts a Time-of-Flight Secondary Ion Mass Spectrometry (ToF-SIMS), a X-ray Photoelectron Spectroscopy (XPS/ESCA), a Fourier Transform Infrared Spectroscopy (FT-IR), a FT-IR microscope, a Confocal Raman System, and a Veeco Dektak 150 high resolution stylus surface profiler. Keck-II is open to all the faculty and students at Northwestern University as well as the researchers at the nearby academic institutions and related industrial companies. Keck-II center operates and functions like its sister facilities (NIFTI and EPIC)-based on the core philosophy of open-access, hand-on training, collaboration and assistance from our staff. Training is offered on a periodic basis, both as “crash courses” or part of hands-on structured courses, as well as individual ad-hoc training as need arises. ---- Dektak 150 Stylus Surface Profiler, FT-IR , Time-of-Flight Secondary Ion Mass Spectrometry , Confocal Raman Microscope System, X-ray Photoelectron Spectroscopy, FT-IR microscope

Nanoscale Integrated Fabrication, Testing and Instrumentation Center - NIFTI - part of NUANCE Northwestern University 2220 Campus Dr Cook Hall 2036 (mail), 1133 (ofc) Evanston, IL 60208-3108 (847) 467-2318 nuance@northwestern.edu View Website Primary Contact: Gajendra Shekhawat (847) 491-3204 g-shekhawat@northwestern.edu Last Updated: 11/12/2010	Services offered: Atomic Force Spectroscopy Cell Imaging Microscopy Optical Imaging Ultrasonic Imaging Characterization, Image Analysis, Imaging, Spectroscopy Associations: MWACD
Our three facilities, EPIC, NIFTI, and Keck-II, are committed to- enhancing education & research by providing a prudent combination of state-of-the-art and core surface science analytical characterization instruments assisting users by offering hands-on instrument skills training & up-to-date information on concepts, and to collaborate in classroom instruction and research activities *developing innovative research programs and cutting-edge techniques for atomic and nanoscale materials characterization ---- JEOL JSPM 5200 microscope, NanoInk NScriptor Nanopatterning System, Nanonics Near Field Optical Microscope , Digital Instruments, Nanoscope MultiMode Scanning Probe Microscope , SPM, Spectra-Physics Femtosecond Laser, TriVista Confocal Raman Microscope System, Veeco BioScope II Biological Scanning Probe Microscope and Veeco high performance dimension ICON system

Flow Cytometry Core Facility - Cancer Center Northwestern University 710 North Fairbanks Court Olson Pavillion, Room 8-505 Chicago, IL 60611 (312) 908-1294 j-marvin@northwestern.edu View Website Primary Contact: James Marvin (312) 503-5923 j-marvin@northwestern.edu Last Updated: 11/12/2010	Services offered: Cell Sorting Data Analysis DNA Analysis Flow Cytometric Analysis Protocol Development/Clinical Trial Coordination Consultation, Data Storage/Off-Line Analysis, Flow Cytometer, Sample Preparation, Training Associations: Cancer Center MWACD
...The Flow Cytometry Core Facility provides cell sorting services and access to routine flow cytometry assays such as immunophenotyping and DNA analysis as well as guidance, technical assistance and equipment for the investigators to utilize more complex mult-parametric, multi-laser measurement and cell sorting in their research. The recent acquisition of the MoFlo high-speed sorter has increased the facilities technical capabilities. ---- Accuri C6, BD LSRII, BD FacsAria, BD Fortessa, Beckman Coulter Cyan, Beckman Coulter Epics XL-MCL, Beckman Coulter Moflo, Beckman Coulter ViCell, Cell counting, Complex Multi-parametric Analyses (8 color or more), Consultation on Experimental Design/Data Interpretation/Troubleshooting, Data Analysis, FACS, High Speed Cell Sorting, Immunophenotyping, Individual training on each instrument, New Assay Development, Off-line Computer Workstations, Sample Preparation

Keck Biophysics Facility Northwestern University 2220 Campus Drive Cook Hall 4th Floor, Rooms 4118 and 4106 Evanston, IL 60208 (847) 491-3972 keck.biophysics@gmail.com View Website Primary Contact: Arabela Grigorescu (847) 492-7610 a-grigorescu@northwestern.edu Last Updated: 11/12/2010	Services offered: Assays and Measurements Light Scattering Molecular Imaging Phosphor Imaging Proteomics Real-time qPCR Spectroscopy Analytical, Characterization, Fluorescence/Photonic Microscopy, Imaging, Other, Phosphoimager & Fluorescence Scanner, Spectroscopy Associations: Cancer Center MWACD
The Keck Biophysics Facility provides researchers with 24-hour access to a collection of advanced instruments for biophysical and biochemical characterization of macromolecules and their interactions. Training, technical expertise and assistance are provided by the facility personnel. Staff service on selected instruments is available. ---- Analytical Ultracentrifuge - XLA & UltraScan AUC XLA, Circular Dichroism Spectrometer , CD, Differential Scanning Calorimeter , DSC, Dynamic Light Scattering Instrument , DLS, Fermentor 1.25L, Fermentor 15L, Fluorescence Photon-Counting Spectrofluorimeter PC1, Fluorescence Plate Reader , Fluorescence Polarization , Beacon, Fluorometer K2 - Time Resolved Fluorescence Spectrometer, Gel Imager UV-VIS , Kodak, HPLC, Isoelectric Focusing Gel Electrophoresis, Isothermal Titration Calorimeter , ITC, Phosphoimager/fluoroimager, Storm, Rapid Quench Flow Instrument, Real Time PCR, Rotor Type 45Ti, Stopped-flow Spectrometer ( UV-VIS Absorbance , Fluorescence ), UV/VIS DAD Spectrophotometer, UV/VIS/NIR Spectrophotometer with Diffuse Reflectance Accessory

Molecular Genetics Core Facility Children's Hospital Boston 300 Longwood Avenue CLS Building 15th Floor Boston, MA 02115 (617) 919-3380 genome@dnacore.org View Website Primary Contact: Hal Schneider (617) 919-3380 genome@dnacore.org Last Updated: 11/12/2010	Services offered: BioBanking Copy Number Variation (CNV) DNA Analysis Genomics Genotyping Mass Spectrometry Microarray Molecular Library Services Nucleic Acid Extraction Real-time qPCR RNA analysis RNA Integrity Sequencing - Capillary Sequencing Sequencing - DNA Sequencing Sequencing - Next-Gen Sequencing DNA/RNA Extraction Associations: ABRF
The overall objective of the Molecular Genetics Core Facility is to provide a location where researchers can have access to high quality, low cost genomic technology services and expertise in a timely, affordable manner. The services offered include DNA sequencing, Next-Gen sequencing, Affymetrix and Illumina whole-genome expression and genotyping microarray technology, microsatellite genotyping, high-throughput qPCR and SNP services on the Fluidigm Biomark System, multiplex SNP genotyping on the Sequenom MassARRAY, and sample extraction and banking. The MGCF also assists investigators with project design and collaborates on linkage and association study analysis. ---- We are open to collaborations and can assist in project design for any of the services we provide.

Instrument Shop - Evanston Northwestern University 2145 Sheridan Road Tech Room NG-40 Evanston, IL 60208 (847) 467-4315 j-sundwall@northwestern.edu View Website Primary Contact: Jeffrey Sundwall (847) 467-4315 j-sundwall@northwestern.edu Last Updated: 11/12/2010	Services offered: Fabrication Support Services - Electronics & Fabrication Shop Machine Shop Associations: MWACD
...The Northwestern University Instrument shop is a full service machining, fabrication and repair facility capable of meeting the needs of an active research community. We support researchers in the physical and life sciences. The shop is staffed by four highly talented instrument makers with over 150 years experience. The Instrument Shop is a fully equipped machine shop outfitted with manual and CNC (computerized numerical control) machines. ---- Autocad -- Design Software, Autodesk Inventor -- 3-D Design Software, Brazing -- Torch brazing of all metals, Bridgeport -- Milling machine, CAD-CAM -- Software & programming for CNC machines, EDM -- Electrical Discharge Machine, Electrophoresis -- Repair, Fasteners -- Screws , Nuts , Bolts , Washers, Hardinge -- Engine Lathe, Helium Leak Detection -- Alcatel Helium Mass Spectrometer Leak Detector, MALDI -- Plates for Bruker MTP, Machining CNC Manual, Mechanical Design -- Autocad , Inventor , Solidworks, Metals -- Copper Brass Stainless Steel Alloy Steel Aluminum Monel Inconel Titanium Lead Silver Gold Platium Niobium Invar, Microscope Threads -- RSM , Thorlab , Special taps for microscope and camera mounts, Model Shop -- Provides design & fabrication of custom scientific apparatus, Orbital Welding -- Orbital welding of cajun fittings and tubing -- VCR & VCO, Pipe Fittings -- All types of brass & stainless steel pipe and tubing fittings, Plastic -- acrylic polycarbonate delrin nylon, Sample -- holders, polishing fixtures, test bars, preparation,, Soldering -- Silver soldering and soft soldering all metals, SwageLoc, Turning -- Lathe work , CNC and manual, Vacuum -- Fittings , KF , Conflat , Chamber Design , Chamber Fabrication, WEDM -- Wire Electrical Discharge Machine, Welding -- Oxy-Acetylene , Silver Soldering , Brazing , Soft Soldering, Welding -- TIG Tungsten Inert Gas , MIG Metal Inert Gas, Woodworking -- Cutting , sawing , drilling

SD BRIN Bioinformatics Core Facility University of South Dakota 414 E Clark St Vermillion, SD 57069 (605) 677-5388 csci@usd.edu View Website Primary Contact: Carol Lushbough (605) 677-5388 carol.lushbough@usd.edu Last Updated: 11/12/2010	Services offered: Computational - Bioinformatics Computational - Biostatistics Associations: INBRE
We are working on a variety of projects in conjunction with the USD Sanford School of Medicine. This collaboration is intended to get computer science students involved in bioinformatics research at both the graduate and undergraduate levels. Students in our department are involved in developing the infrastructure necessary to carry on research in bioinformatics by creating software solutions to fit the needs of faculty involved in this field of research. These projects are funded through external grant sources. ---- Large computing cluster, BioExtract Server, BioStream, Table Maker

shRNA Library Core UMass Medical School 55 Lake Ave North Worcester, MA 01655 (508) 856-4252 shrnaclone@umassmed.edu View Website Last Updated: 11/12/2010	Services offered: Provides Clones Associations: NERTRN
UMMS has purchased the complete collections of human and mouse retroviral and lentiviral short hairpin RNA (shRNAmir) libraries from Open Biosystems, and has negotiated with them to distribute individual clones to UMASS researchers (ONLY) at a reduced price.

Flow Cytometry Resource Center (FCRC) Rockefeller University 1230 York Avenue, Box 344 New York, NY 10065 (1-2) 327-7657 mazels@rockefeller.edu View Website Primary Contact: Svetlana Mazel (1-2) 327-7656 mazels@rockefeller.edu Last Updated: 11/12/2010	Services offered: Cell Imaging Cell Sorting Flow Cytometric Analysis
State-of-the-art Flow Cytometry Resource Center. Cell sorting and ImageStream-X analysis are performed for extended hours by staff. Analysis offered as self service with 24/7/365 access. ---- BD FACSAria I-to-III (4 lasers/13 colors) BD FACSAria II (5 lasers/16 colors) BD FACSAria SORP (6 lasers/18 colors) ImageStream-X (4 lasers/9 colors) BD LSRII-1 (5 lasers/16 colors, FACSFlow, HTS) BD LSRII-2 (4 lasers/14 colors, FACSFlow, HTS) FACSCalibur-1 (1 laser/3 colors) FACSCalibur-2 (2 lasers/4 colors)

Biophysical Core University of Connecticut Health Center L3004/MC3305, MMSB, U Conn Health Center 263 Farmington Avenue Farmington, CT 06030-3305 (860) 679-2885 liluo@uchc.edu View Website Primary Contact: Dr. Jeffrey Hoch (860) 679-3566 hoch@uchc.edu Last Updated: 11/11/2010	Services offered: Mass Spectrometry Support Services - Shared Instrumentation Oversight & Maintenance Associations: NERTRN
The Biophysical Core maintains the following instruments: (1) A Jasco J-715 spectropolarimeter for both near- and far-UV circular dichroism studies. The instrument contains a Peltier thermostated sample holder for thermal melts. (2) MicroCal VP Capillary Differential Scanning Calorimeter. DSC measures the enthalpy (∆H) of unfolding due to heat denaturation. A biomolecule in solution is in equilibrium between the native (folded) conformation and its denatured (unfolded) state. The higher the thermal transition midpoint (Tm), when 50% of the biomolecules are unfolded, the more stable the molecule. DSC measures the change in heat capacity (ΔCp) of denaturation. (3) Microcal Isothermal Titration Calorimeter to assist in calculating stochiometry, binding constant, enthalpy and entropy. The instrument includes an internal Peltier mechanism. (4) Wyatt MiniDawn Triple-Angle Light Scattering Detector (MALS) for measuring the molecular weights, sizes, and conformations of macromolecules in solution. (5) Voyager DE-pro MALDI-TOF Mass Spectrometer for accurate determination molecular weights at subpicomolar range. The system is used for routine non-expert operation. It incorporates Delayed Extraction™ technology for excellent sensitivity, mass accuracy, and resolution. (6) Hitachi F-2500 Spectrofluorometer which offers high sensitivity, variable spectral bandwidth and scanning speeds of up to 12,000nm/min. Its horizontal beam optical design provides a high concentrated light beam along with low sample volume requirements. It offers Excitation/Emission Scan, Time Based Measurements, Pre-Scan, and Data Export to Excel.

CHOP Protein and Proteomics Core Children's Hospital of Philadelphia Children's Hospital of Philadelphia Research Institute 3615 Civic Center Boulevard, ARC/806 Philadelphia, PA 19104 (267) 426-5551 seeholzer@email.chop.edu View Website Primary Contact: Steven Seeholzer Last Updated: 11/11/2010	Services offered: Mass Spectrometry Protein Extraction/Purification Proteomics SDS-PAGE Spectroscopy Phosphoproteomics, SILAC quantitative protein profiling, Protein Expression, Protein Purification, Circular Dichroism, Analytical Ultracentrifugation, Surface Plasmon Resonance
The Children’s Hospital of Philadelphia (CHOP) Protein Core Facility provides a variety of complementary techniques that enable CHOP researchers to produce and characterize proteins, investigate protein-protein interactions, and characterize whole proteomes. The core facility is located in the Joseph Stokes Jr. Research Institute (806 Abramson Research Center) and features protein expression and purification, protein characterization, protein-protein interactions, and proteomics approaches. Some services are provided on a user-operated, sign-up basis, while others are performed as full-service by the dedicated facility personnel. Protein expression services include recombinant protein production in both bacteria and insect cells. HPLC and FPLC equipment, columns and resins are available for a variety of purification needs. Endogenous fluorescence, circular dichroism, analytical ultracentrifugation, and surface plasmon resonance can be used to characterize the folding status of proteins and their interactions with small molecules and other proteins. Biomarker discovery in various biofluids can be undertaken with the SELDI mass spectrometer along with our other research grade mass spectrometry equipment. A wide range of proteomics experiments are possible. These include but are not limited to: intact mass determination, post-translational modification analysis, protein identification, targeted (e.g. co-immunoprecipitation) or comprehensive (e.g. protein expression profiling) proteome analysis. Proteome analysis may employ a number of different isotope-labeling strategies. Workflows for these experiments are unique to each project and can involve a range of multi-dimensional separation techniques coupled to the appropriate MALDI or ESI mass spectrometers in the lab. Additional information can be found on our web page: http://stokes.chop.edu/cores/protcore/. ---- Protein expression/purification ---- Children’s Hospital of Philadelphia Research Institute Protein and Proteomics Core Facility: The CHOP Protein and Proteomics core is located in a 2000 sq. ft. laboratory on the 8th floor of the Abramson Research Center. The lab contains all of the resources of the CHOP Protein Core Facility. Physical space comprises benches, 2 chemical hoods, a refrigerated Gem box, tissue culture room and three equipment rooms, all adjoining the lab. The usual range of equipment needed for protein production and biochemical and cell biological experiments is available. The lab is also very well equipped with the more specialized instrumentation and computational capabilities necessary for proteomics experiments. Major pieces of equipment in the lab include: • Mass Spectrometers – Thermo LTQ, Thermo LTQ-Orbitrap XL • Ion Sources – 2ea. New Objective PicoView nano-ESI sources, ADVION NanoMate 96 well ESI source, Thermo micro-ion sources, Michrom Bioresources CaptiveSpray nanoESI source. • Heraus HERAsafe laminar flow hood, Erlab CaptairBio biosafety hood • 2x New Brunswick InnOva4230 refrigerated incubator/shakers, New Brunswick C24KC refrigerated incubator/shaker • BiaCore 3000 Surface Plasmon Resonance • Beckmann-Coulter Optima XL analytical ultracentrifuge • HPLC equipment – Beckman-Coulter Proteome Lab PF2D, 2ea. Eksigent nano LC2D HPLC systems with autosampler (dedicated to mass spectrometers), Shimadzu HPLC system (5 to 400 uL/min), Beckman-Coulter Gold HPLC system (0.2 to 2 mL/min, currently dedicated to hydrophyllic interaction chromatography large scale phosphoproteome projects), GE Amersham Biosciences AKTA FPLC system with wide range of chromatographic media • Agilent 8453 UV-Vis spectrophotometer, Nanodrop spectrophotometer Computing Infrastructure & Software: In addition to the computers dedicated to instrument control and data acquisition the lab is equipped with an additional 8 Windows XP workstations and servers. Four of these have fully licensed Thermo Xcalibur with Bioworks, Proteome Discoverer, and SEQUEST database search software installed. Another 64bit quad-core dual CPU Windows XP server is dedicated to database searching with Matrix Science MASCOT software. APEX Quantitative Proteomics Tool (J. Craig Venter Institute) is implemented for label-free quantification experiments. The SageN Sorcerer2 5 CPU LINUX cluster is dedicated to SEQUEST database searches and calculation of probability of phosphorylation site localization using the ASCORE algorithm. Another dual-core dual cpu Windows XP workstation is used primarily for MaxQuant and MSQuant processing of SILAC, iTRAQ and iCAT quantification experiments as well as phosphoproteome and other large scale post-translational modification experiments. The Institute for Systems Biology Trans-Proteomic Pipeline software is fully implemented on most of our WinXP workstations and the LINUX cluster. All laboratory computers are Ethernet connected and we have all the space we require (presently 6 Terabytes) on our institutional Storage Area Network for data archiving and secure redundant backup. In addition to the core’s private archive area, the CHOP Research Institute provides us with a Content Management System (Confluence) allowing us to collaboratively share files and information among groups, assisting with various aspects of project management.

Advanced Light Microscopy & Image Analysis Core New York State Dept. of Health PO Box 509 Empire State Plaza Albany, NY 12201 (518) 474-7048 rcole@wadsworth.org View Website Primary Contact: Richard Cole rcole@wadsworth.org Last Updated: 11/11/2010	Services offered: Assays and Measurements Cell Culture Cell Imaging Confocal Microscopy Immunohistochemistry Optical Imaging Tissue Culture Associations: ABRF
Adherent cells or tissue fragments can be imaged in a variety of methods depending on the question being asked. If drug delivery is required, a perfusion style chamber is used; if gas exchange for the growth media is needed glass bottom Petri dishes can be used; if long term (> 3 days) sterility is needed, there are sealed Rose chambers for imaging. Regardless of what the experimental design, the specimen can be maintained from RT to 50 ºC. Non-destructive image modes i.e. phase contrast, differential interference contrast or polarized light coupled with shutter and filtered illumination provide as close to homeostatic conditions as possible. Once the images are collected there is an extensive array of image processing & analysis software to assist in data analysis/interpretation. These “tools” range from the simple, contrast manipulation to complex tracing and counting algorithms. There are also different forms of deconvolution available for "removing" the blur from fluorescent specimens, or transmitted light images. If multiple molecules are labeled, co-localization can be determined for either a static image or an image sequence. There are both automatic and semi-automatic 2 and 3-D tracking/montaging algorithms available. ---- Fluorescence imaging: Multiple labeled biomolecules such as GFP etc. or fluorophore can be imaged in four-dimensions. This can be done over time (5th dimension) for live specimens as well as statically for fixed specimens. Specimens can be as thin as a single cell or as thick as a whole organism (i.e. drosphilia) or anything in between. Conventional two-dimensional imaging can be done at high sensitivity, i.e. low bleaching rates. Three-dimensional imaging can be done in wide-field for relatively thin specimens or by confocal microscopy for thick specimens.

Flow Cytometry UMass Medical School 55 Lake Ave North S5-322 Worcester, MA 01655 (508) 856-3276 x 5088561598 Richard.Konz@umassmed.edu View Website Primary Contact: Richard Konz (508) 856-1598 Richard.Konz@umassmed.edu Last Updated: 11/11/2010	Services offered: Cell Imaging Cell Sorting Confocal Microscopy Data Analysis Flow Cytometric Analysis Microscopy Optical Imaging Associations: NERTRN
The Flow Cytometry Facility provides a wide variety of cytometry services for internal and external investigators with state-of-the-art cell sorting and analyzing capabilities. We currently have three FACSCailburs, one 4 laser and one 5 laser LSRIIs, a 5 laser FACSAria cell sorter, a 3 laser Digital Dako MoFlo XDP hi-speed cell sorter, an all digital 3 laser 11 color FACSVantage SE DiVa cell sorter and a 14 color, 4 laser FACSAria dedicated to BSL3 cell sorting. One LSR II and one Calibur are dedicated for customer use, after training, which we can provide.

Microscopy and Imaging Cornell University B46 Weill Hall Ithaca, NY 14853 (607) 254-4860 mifbiotech@cornell.edu View Website Primary Contact: Carol Bayles (607) 254-4860 mifbiotech@cornell.edu Last Updated: 11/11/2010	Services offered: Cell Imaging Confocal Microscopy Flow Cytometric Analysis Microscopy Molecular Imaging Optical Imaging Ultrasonic Imaging Fluorometry, Image analysis Associations: ABRF Cancer Center
Facility provides instrumentation and training in the following areas: Laser Scanning Confocal Microscopy Wide-field fluorescence microscopy Live Animal Imaging Ultrasound Fluorimetry Flow Cytometry ---- Zeiss 710 confocal microscope with incubation Visual Sonics Vevo 770 high-resolution ultrasound for small animal imaging Volocity 3D imaging software

Cell Imaging Facility Northwestern University Feinberg School of Medicine 300 E. Superior Ave. Chicago, IL 60611 (312) 503-3429 t-chew@northwestern.edu View Website Primary Contact: Pam Reid (312) 503-3429 Last Updated: 11/11/2010	Services offered: Cell Imaging Confocal Microscopy Laser Capture Microdissection Microscopy Optical Imaging Support Services - Biological photography/Photomicrography Support Services - Shared Instrumentation Oversight & Maintenance Associations: MWACD ABRF
The Cell Imaging Facility is located on the second floors of Morton and Searle Buildings at Northwestern University Feinberg School of Medicine, in downtown Chicago. Covering a sixteen-room suite of ~2,300 sq. ft, the Cell Imaging Facility provides state-of-the-art light and electron microscopic instruments and consultation to researchers at the Medical School, the Evanston main campus, as well as to users at Children's Memorial Research Center (CMRC). ---- Atom Probe Tomograph (APT), Confocal Microscopy, Cryo/Low-Temp Preparation, Electron Microscopy, Film Scanning, Fluorescence/Photonic Microscopy, Image Analysis, Imaging, Imaging Software, In vivo Animal Imaging , Input/Output, Light Microscopy, Microarray Data Analysis, Microinjection, Spectroscopy, Training ---- AutoQuant X for deconvolution, Baltec Rotary Shadower, Correlative light and electron microscopy, CLEM, Critical point drying, Denton Vacuum Evaporator, Digital and conventional EM image acquisition, Electron microscopy of tissue and cultured cell specimens, Eppendorf FemtoJet injector on Zeiss Axiovert fluorescence microscope, FRAP, FRAP-FLIP Calc, FRET, Fluorescence-based minimally invasive small animal intravital imaging, Fluorescence-based non-invasive small animal imaging, FujiFilm FLA-5100 Imager, Full service sample preparation and sectioning, HistoQuest, ImageJ, Immunogold EM, Individual training on each instrument, JEOL 100CX transmission electron micr, JEOL 1220 transmission electron microscope, Leica UC6 Ultramicrotome , Leica Ultracut E Ultramicrotome, Leica Ultracut UCT Ultramicrotome, MetaMorph, Microinjection, Narishige Needle Puller, Needle puller, Nikon C1Si spectral laser scanning confocal, Olympus IV-100 Intravital imaging system , Olympus OV-100 Whole-mouse imager , Phosphoimager and gel scanning, Photoshop, Platinum-replica electron microscopy, Sputter-coating, TissueGnostics Tissue/Cell High Throughput Imaging and Analysis System, TissueQuest, Volocity, Zeiss Axioskop w/ CRi Nuance spectral camera, Zeiss LSM 510 META laser scanning confocals (two systems), Zeiss LSM software, Zeiss Zen software

Office of Animal Care Management University of Vermont 116 Hills Building 105 Carrigan Drive Burlington, VT 05405 (802) 656-0459 keirsten.rapoza@uvm.edu View Website Primary Contact: Keirsten Rapoza (802) 656-0459 drruth@uvm.edu Last Updated: 11/11/2010	Services offered: Animal Husbandry Veterinary Services Associations: NERTRN
The Office of Animal Care Management oversees the operation of research animal facilities at the University of Vermont. The University's research animal facilities currently house approximately 10,000 animals of which over 98% are mice and rats. The majority of the research rodents are high-health sta

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