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The Center for X-Ray Crystallography is the hub for high-resolution
structural biology at the University of Vermont. X-ray crystallography
allows biological and biomedical researchers to visualize proteins, RNA,
DNA and their complexes at atomic resolution. The molecular details of
specimens as small as DNA binding domains and as large as the ribosome have
been elucidated via this powerful method. The CXX provides resources for
all stages of macromolecular structure determination:
- screening of samples for 'crystallizability' via dynamic light
scattering
- automated high-throughput (robotic) screening for crystallization
conditions
- crystal incubation at various temperatures from 4C to 30C
- preparation of heavy-atom and anomalous derivatives (including
high-pressure noble gas infusion)
- collection and reduction of x-ray diffraction data (cryogenic or
room temperature)
-
solution and analysis of structures with state-of-the-art clustered
computing hardware
Unlike the crystallographic laboratories at most other universities, the
CXX is run as a facility available to researchers across the UVM campus who
wish to add high-resolution macromolecular structural characterization to
their research program. The CXX is located in rooms E312-E316 of the Given
Building. For further information, contact:
- Mark Rould (CXX Director) 6-9532, mrould@uvm.edu
- Sylvie Doublie (CXX Supervisor) 6-9531, sdoublie@uvm.edu
- Stephen Everse (CXX Supervisor) 6-8271, severse@uvm.edu
- Kelvin Chu (CXX Supervisor) 6-0064, kchu@uvm.edu
The CXX operates within guidelines set by the UVM Radiation Safety
Office and the UVM Environmental Safety Office.
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