Arabidopsis DNA micro-preps for PCR

From Klimyuk et al (1993), Plant Journal 3, 493.

Reagents:

Procedure:

Clip a small piece of leaf tissue (2mm x 2mm) into an eppendorf tube containing 50 ul of 0.25 M NaOH and macerate in NaOH.

DNA can be stored short-term @ 4C, but should be boiled 2 min before using again.

 

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