University of Vermont

UVM College of Medicine Mass Spectrometry Facility

Mass specs at UVM

UVM Mass Spectrometry Facility

Waters Xevo G2-XS QTOF LCMS

  • Mass separation by a quadrupole (Q) mass selection of m/z = 20-4000 & 20-16,000 (non-resolving) and time of flight (TOF) m/z = 20-100,000 mass ranges
  • XS collision cell for collision-induced dissociation (CID)
  • TOF high resolution of 40,000 FWHM
  • CID by MS/MS and MSE
  • ESI and nanospray-ESI interfaces for liquid sample introduction
  • Waters Nanoacquity UPLC system
  • Controlled by the Waters MassLynx data system on a PC
  • The system processes peptide data using Waters ProteinLynx Global Server (PLGS) and Progenesis and metabolomics data by Progenesis
Waters Xevo G2-XS

ABI Sciex 4000QTrap Pro LCMS

  • High performance hybrid triple-quadrupole/linear ion trap mass spectrometer with collision induced dissociation (CID) capability for MS/MS and for MSn using the ion trap. The ABI 4000QTrap has both the scanning functions of a triple quadrupole with the scanning functions of a linear ion trap that can perform simultaneously in both modes within a single injection/analysis automatically through the software.
  • Operation in both negative and positive ion mode
  • mass range of m/z = 5 to 2,800 in Q1 and Q3 in RF/DC mode and m/z = 70 to 2,800 in linear ion trap mode
  • maximum scan speed of 2,400 amu/sec in RF/DC mode and user-settable scan speeds of 250 amu/sec, 1000 amu/sec and 4,000 amu/sec in linear ion trap mode
  • A number of operational modes are available, including product ion scanning for structural elucidation and precursor ion and neutral loss scanning
  • Three modes of LC spray ionization: (i) atmospheric pressure chemical ionization (APCI), (ii) "Turbo IonSpray", i.e. ESI, and (iii) Nanospray ESI
  • The LC interfaced to the 4000QTrap a Shimadzu with nano-LC capability
  • A full suite of instrument control and data processing software are available
ABI 4000 Qtrap LCMS

Hewlett Packard HP-5973 Gas Chromatograph - Mass Spectrometer (GCMS)

  • HP6890 GC with an HP7683 autosampler
  • Quadrupole mass analyzer; m/z = 0-800 mass range
  • Electron impact (EI) ionization
  • Negative (NCI) and positive chemical ionization (PCI)
  • Used for measuring stable isotopic enrichments in metabolites of biological samples in the range of 0.5 -10 mole % excess (mpe) tracer by selected ion monitoring (SIM).

Varian Saturn 2100T GCMS

  • Varian 3900 GC and CP-8400 autosampler
  • Ion trap with a m/z = 10-650 mass range
  • Operates in electron impact ionization (EI) and postive chemical ionization (PCI) modes
  • Especially useful in PCI mode is the collisionally induced dissociation (CID) option to acquire MS/MS spectra
Varian Saturn 2100T ion trap

Last modified August 04 2017 10:55 AM