UNIVERSITY OF VERMONT
SCHOOL OF ENGINEERING
Mechanical Engineering Seminar Series
Dr. Darcy E. Wagner
College of Medicine -- Pulmonary
Vermont Lung Center
DEVELOPMENT OF THREE-DIMENSIONAL SCAFFOLDS OF ACELLULAR HUMAN AND PORCINE LUNGS FOR HIGH THROUGHPUT STUDIE
Acellular scaffolds from complex whole organs such as lung are being increasingly studied for /ex vivo/ organ generation and for /in vitro/ studies of cell-extracellular matrix interactions. We have established effective methods for manufacturing acellular scaffolds from large animal and human lungs. Further, we have developed techniques which allow multiple small segments (~1-3cm^3 ) to be excised that retain 3-dimensional lung structure. Coupled with the use of a novel synthetic pleural coating, cells can be selectively physiologically inoculated via preserved vascular and airway conduits. Inoculated segments can then be further sliced for high throughput studies. Additionally, we have used thermography as a powerful noninvasive technique for monitoring perfusion decellularization and for evaluating preservation of vascular and airway networks following human and porcine lung decellularization. Collectively, these techniques are a significant step forward as they allow high throughput /in vitro /studies from a single lung or lobe in a more biologically relevant, three-dimensional acellular scaffold.
Dr. Wagner received her undergraduate degree in mechanical engineering from Gonzaga University and she received her doctorate in Biomedical Engineering from the University of Toledo in 2011. Her doctoral work focused on novel uses of microwave synthesis methods for calcium phosphate nanoparticles and their potential uses in biomedical applications, with a focus on scaffold design for orthopedic tissue engineering and gene delivery. Dr. Wagner is currently a National Institute of Health (NIH) Postdoctoral Fellow and works at the UVM in the Vermont Lung Center's Weiss Laboratory. Her current researchfocuses on manufacturing acellular scaffolds for lung tissue engineering and the biological mechanisms by which cells interact with their physical environment.
September 20, 2013
Last modified September 18 2013 01:33 PM