home
DeltaVision Description
DeltaVision image Morielli

DeltaVision Restoration
Microscopy System:
Description and Capabilities

 
Fixed HEK293 cell with red labelled potassium channel, blue labelled actin filaments and green labelled cortactin in a projection near the coverslip. Courtesy of Tony Morielli.

The DeltaVision Restoration Microscopy System consists of a set of precision motors and a specially designed stage attached to an inverted Olympus IX70 microscope.† The provided deconvolution software allows the resolution of deconvolved images to exceed the Raleigh limit.† Instrument and software are designed to provide time-lapse capabilities in live cells or tissues (<100 micron thick).

Cameras

Optical Components

  • Objectives: 20x air with DIC optics, 40x oil, 60x oil, 100x oil, 60x water
  • 1.5x auxiliary magnification
  • 100 W Mercury arc lamp
  • Excitation Filters: 380-380 nm (DAPI), 431-441 nm (CFP), 480-500 nm (FITC), 490-510 nm (YFP), 541-569 nm (rhodamine/texas red/Cy3), 630-650 nm (Cy5)
  • Emission Filters: 432-482 nm (DAPI), 455-495 nm (CFP), 509-547 nm (FITC/fura-2), 520-550 nm (YFP), 580-654 nm (rhodamine/texas red/Cy3), 665-705 nm (Cy5)
  • Ratiometric calcium imaging with fura-2 (excitation filters at 340 and 370 nm with 509-547 nm emission)

Accessories

  • Environmental Chamber
    • For use with the DeltaVision system
    • Encloses the entire stage of the microscope including the objectives
    • Allows temperature regulation between 32 and 42 degrees Celsius
    • CO2 enrichment attachment allows for experiments performed in carbonate buffers

  • Microperfusion Bath Perfusion
    • 8-channel perfusion allows for rapid application of chemicals in a localized region of interest
    • Rapid cycling reduces desensitization of cells to various chemicals
    • Experiments can be synchronized with pClamp software

    Last modified February 11 2008 12:25 PM

    Contact UVM © 2014 The University of Vermont - Burlington, VT 05405 - (802) 656-3131