DeltaVision Restoration
Microscopy System:
Description and Capabilities

Fixed HEK293 cell with red labelled potassium channel, blue labelled actin filaments and green labelled cortactin in a projection near the coverslip. Courtesy of Tony Morielli.

The DeltaVision Restoration Microscopy System consists of a set of precision motors and a specially designed stage attached to an inverted Olympus IX70 microscope.  The provided deconvolution software allows the resolution of deconvolved images to exceed the Raleigh limit.  Instrument and software are designed to provide time-lapse capabilities in live cells or tissues (<100 micron thick).

Cameras

• For speed: Photometrics Coolsnap HQ camera (using interline Sony ICX285 CCD)
• For highest resolution: CH350E camera (using Kodak CCD KAF1401E)

Optical Components

• Objectives: 20x air with DIC optics, 40x oil, 60x oil, 100x oil, 60x water
• 1.5x auxiliary magnification
• 100 W Mercury arc lamp
• Excitation Filters: 380-380 nm (DAPI), 431-441 nm (CFP), 480-500 nm (FITC), 490-510 nm (YFP), 541-569 nm (rhodamine/texas red/Cy3), 630-650 nm (Cy5)
• Emission Filters: 432-482 nm (DAPI), 455-495 nm (CFP), 509-547 nm (FITC/fura-2), 520-550 nm (YFP), 580-654 nm (rhodamine/texas red/Cy3), 665-705 nm (Cy5)
• Ratiometric calcium imaging with fura-2 (excitation filters at 340 and 370 nm with 509-547 nm emission)

Accessories

• For use with the DeltaVision system
• Encloses the entire stage of the microscope including the objectives
• Allows temperature regulation between 32 and 42 degrees Celsius
• CO₂ enrichment attachment allows for experiments performed in carbonate buffers

• 8-channel perfusion allows for rapid application of chemicals in a localized region of interest
• Rapid cycling reduces desensitization of cells to various chemicals
• Experiments can be synchronized with pClamp software