home
Nikon TIRF

The Nikon TIRF – Total Internal Reflection Fluorescence Microscope

This system is designed to observe fluorescent signals in a thin region of samples adherent to a glass coverslip. An evanescent wave generated by TIR excites ~200nm above the glass/water interface, allowing observation of cell-surface phenomena.  

nikonMicroscope

Nikon TE2000U:

  • LUDL motorized filters
  • Perfect focus module
  • Conventional metal halide epifluorescence

Lenses:

  • Plan Apo TIRF 60X/1.45NA Oil lens with correction collar
  • Nikon Plan Apo 20X/0.77

Laser

Coherent 50 mW 488 nm OPSL Sapphire laser

Camera

Photometrics Cascade 512B EMCCD

Computer

HP Compaq 8100 Elite 64-bit Workstation

Physiology Equipment

Axopatch 200B Integrating Patch Clamp

Last modified December 02 2011 10:48 AM

Contact UVM © 2014 The University of Vermont - Burlington, VT 05405 - (802) 656-3131