2. When daily work is completed, wash work spaces with Vesphene.
3. Keep hands and fingers away from face and mouth.
DO NOT chew pencils, put pens in your
mouth, rub eyes or nose, etc.
4. Absolutely NO smoking, drinking or eating in the laboratory.
Cups, bottles, dishes and food
should be placed in backpacks or otherwise
protected before entering the laboratory.
5. Wash hands before leaving the lab, every time you leave.
This includes brief absences for getting
a drink of water or using a rest room.
Be sure to use the foot pedals to operate the water flow in
the sink. Using the hand controls may
result in contamination of the hand controls.
6. At all times while in the laboratory, wear a knee length,
long sleeve lab coat. The lab coat must be
kept buttoned, snapped or tied shut.
Remove this lab coat even when leaving the laboratory for
brief periods of time. Store the lab
coat in the drawer above your microscope.
7. Keep hair tied back so it will not catch on fire in a
bunsen burner and so it will not fall into petri
dishes or reagents.
8. Do not wear loose or dangle-like jewelry, scarves or
ties, as they may fall into a bunsen burner,
onto a culture plate or into specimens or
reagents.
9. Papers are to be placed on the pull-out shelf.
Papers should never be placed on the bench top
where they may be contaminated by cultures.
10. When working with specimens under the biological safety hood,
it is necessary to wear gloves in
addition to the lab coat. The gloves
are considered contaminated after such use, so immediately
after working under the hood, remove and discard
the gloves in the lidded trash cans lined with
biohazard bags.
While working with the biological safety hood
do not touch anything outside of the hood with
your gloves as the gloves will contaminate
whatever you touch. This includes paper work, bench
tops, pens, pencils, etc.
11. ABSOLUTELY NO mouth pipetting is allowed, NO EXCUSES.
12. Report all accidents to the Instructor or Teaching Assistant, no matter how minor the injury.
13. If there is a spill:
a) If the spill poses a potential
electrical hazard, act immediately to stop the source of the
spill.
Unplug any electrical equipment that may be at risk. If electrical
equipment
becomes wet,
do not touch equipment or liquid near it.
b) If no electricity is involved,
squirt or pour disinfectant (clorox or vesphene) over the spill.
Lay paper towels
on top of the spill. Wait a few minutes. Wearing gloves, use
fresh
paper towels
to pick up the soiled ones. Discard towels and gloves in a large,
round
stainless steel
pot lined with biohazard bags.
14. Inoculating Loops and Needles should be flame sterilized before
and after use. To flame sterile,
follow these directions:
Adjust the bunsen burner flame so there is
a double cone. This should consist of an outer cone
which is light in color and an inner blue
cone. Place the wire of the loop or needle in the bunsen
burner flame just above the point of the blue
cone. Begin flaming at the end of the wire near the
hub of the handle first. As the wire
turns red hot, slowly draw the loop or the tip of the needle
toward the flame. Make sure any material
on the loop chars before you draw the loop or the tip of
the needle into the flame.
Do not insert a loop or needle tip that is
heavily laden with organism or clinical material directly
into a flame. If you do this, the material
will spatter, and you will contaminate yourself, your
neighbor and the bench top.
When done with the bunsen burner, turn it off. An unattended flame is dangerous!
15. Discarding contaminated materials:
Tri-pour Containers on bench tops are for
small, non-glass, disposable items such as: swabs,
tongue depressors.
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Discard Racks on bench top in back of the lab
are for glass tubes larger than 10 x 75 mm, either
screw cap or slip-top.
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Sharp's Containers on bench tops are for glass
slides, flamed hematocrit tubes, small, glass test
tubes and any other type of very small, disposable
glass.
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Lidded Trash Cans lined with biohazard bags
in the back of the lab or near the biological
safety hoods are for large, non-glass,
disposable items such as: plastic petri dishes, contaminated
paper towels or Kimwipes, API strips, serological
pipettes, etc.
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