Ph.D.(2001) Department of Cell Biology, Harvard Medical School; Postdoctoral Fellow (2001-2003) Division of Basic Sciences, Fred Hutchinson Cancer Research Center; Postdoctoral Fellow (2003-2006) Department of Cell Biology, Harvard Medical School; Assistant Professor (2006-) Department of Biology, University of Vermont; Co-Director Vermont Genetics Network Proteomics Facility (2008-)

Office Hours: Please email for an appointment.

Visit the Ballif Lab Website Click Here.

For proteomics and mass spectrometry consultations please email me directly or sign-up online at the VGN proteomics website.

Research in the Ballif lab is focused on elucidating molecular mechanisms of signal transduction with an emphasis on genetically-defined signaling pathways regulating mammalian brain development. In addition to biochemical and cell biological approaches, we employ mass spectrometry-based proteomics as a primary tool to simultaneously monitor hundreds to thousands of proteins and their modifications following acute signal administration or across developmental stages of the murine brain.

Publications:

M.S. Miller, P. Lekkas, J.M. Braddock, G.P. Farman, B.A. Ballif, T.C. Irving, D.W. Maughan and J.O. Vigoreaux. Age Related Changes in Drosophila melanogaster Flight Muscle Structure and Mechanics. Biophysical Journal. In Press.

S. Yoon, S. Shin, Y. Liu, B.A. Ballif, M.S. Woo, S.P. Gygi and J. Blenis. Ran-binding protein 3 phosphorylation links the Ras and P13-kinase pathways to nucleocytoplasmic transport. Molecular Cell. 2008, 29:362-75.

E.C. Connors, B.A. Ballif, A.D. Morielli. Homeostatic regulation of Kv1.2 potassium channel trafficking by cyclic AMP. Journal of Biological Chemistry. 2008,283:3445-53.

B.A. Ballif, G.R. Carey, S. Sunyaev, S.P. Gygi, Large-Scale Identification and Evolution Indexing of Tyrosine Phosphorylation Sites from Murine Brain. Journal of Proteome Research, 2008, 7:311-8.

M.D. Zappaterra, S.N. Lisgo, S.Lindsay, S.P. Gygi, C.A. Walsh and B.A. Ballif. A Comparative Proteomic Analysis of Human and Rat Embryonic Cerebrospinal Fluid. Journal of Proteome Research, 2007, 6:3537-48. Abstract

S. Matsuoka, B.A. Ballif, A. Smogorzewska, E.R. McDonald 3rd, K.E. Hurov, J. Luo, C.E. Bakalarski, Z. Zhao, N. Solimini, Y. Lerenthal, Y. Shiloh, S.P. Gygi, S.J. Elledge. ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage. Science, 2007, 316:1160-6. Abstract

B. Wang, S. Matsuoka, B.A. Ballif, D. Zhang, A. Smogorzewska, S.P. Gygi, and S.J. Elledge. Abraxas and Rap80 form a novel BRCA1 protein complex required for the DNA damage response. Science, 2007, 316:1194-8. Abstract

A. Smogorzewska, S. Matsuoka, P. Vinciguerra, E.R. McDonald 3rd, K.E. Hurov, J. Luo J, B.A. Ballif, S.P. Gygi, K. Hofmann, A.D. D'Andrea, S.J. Elledge. Identification of the FANCI protein, a monoubiquitinated FANCD2 paralog required for DNA repair. Cell, 2007, 129:289-301. Abstract

W.M. Brieher, H.Y. Kueh,B.A. Ballif, and T.J. Mitchison. Rapid, actin monomer insensitive depolymerization of Listeria actin comet tails by cofilin, coronin, and Aip1. Journal of Cell Biology, 2006, 175:315-24. Abstract

B.A. Ballif, Z. Cao, D. Schwartz, K. Carraway III and S.P. Gygi. Identification of 14-3-3epsilon Substrates from Embryonic Murine Brain. Journal of Proteome Research, 2006, 5:2372-79. Abstract

M.K. Holz, B.A. Ballif, S.P. Gygi, and J. Blenis. mTOR and S6K1 mediate assembly of the translation pre-initiation complex through dynamic protein:protein interchange and ordered protein phosphorylation events. Cell, 2005, 123:569-80. Abstract

J. Li, B.A. Ballif, A.M. Powelka, J. Dai, S.P. Gygi and V. Hsu. Phosphorylation of ACAP1 by Akt regulates the stimulation-dependent recycling of integrin ß1 to control cell migration. Developmental Cell, 2005, 9:1-11. Abstract

R. Anjum, P.P. Roux, B.A. Ballif, S.P. Gygi and J. Blenis. The tumor suppressor DAP kinase is a target of RSK-mediated survival signaling. Current Biology, 2005, 15:1762-7. Abstract

B.A. Ballif*, P.P. Roux*, S.A. Gerber, J.P. MacKeigan, J. Blenis and S.P. Gygi. Quantitative phosphorylation profiling of the ERK/p90 ribosomal S6 kinase-signaling cassette and its targets, the tuberous sclerosis tumor suppressors. Proceedings of the National Academy of Sciences USA, 2005,102:667-72. *Equal Contribution Abstract

B.A. Ballif, J. Villén, S.A. Beausoleil, D. Schwartz and S.P. Gygi. Phosphoproteomic analysis of the developing mouse brain. Molecular and Cellular Proteomics, 2004, 3:1093-101. Abstract

P.P. Roux, B.A. Ballif, R. Anjum, S.P. Gygi and J. Blenis. Tumor promoting phorbol esters and activated Ras inactivate the Tuberous Sclerosis Tumor Suppressor Complex via RSK. Proceedings of the National Academy of Sciences USA, 2004, 101:13489-94. Abstract

C.J. Richardson, M. Bröenstrup, D.C. Fingar, K. Jülich, B.A. Ballif, S.P. Gygi and J. Blenis. SKAR is a specific target of S6 kinase 1 in cell growth control. Current Biology, 2004, 14:1540-9. Abstract

B.A. Ballif, L. Arnaud, W.T. Arthur, D. Guris, A. Imamoto and J.A. Cooper. Activation of a Dab1/CrkL/C3G/Rap1 pathway in Reelin-stimulated neurons. Current Biology, 2004,14:606-10. Abstract

L. Arnaud, B.A. Ballif and J.A. Cooper. Regulation of protein tyrosine kinase signaling by substrate degradation during brain development. Molecular and Cellular Biology, 2003, 23:9293-302. Abstract

B.A. Ballif, L. Arnaud and J.A. Cooper. Tyrosine phosphorylation of Disabled-1 is essential for Reelin-stimulated activation of Akt and Src family kinases. Brain Research Molecular Brain Research, 2003, 117:152-9. Abstract

Arnaud L, Ballif BA, Forster E, Cooper JA. Fyn tyrosine kinase is a critical regulator of disabled-1 during brain development. Current Biology, 2003, 13:9-17. Abstract

B.A. Ballif and J.Blenis. Molecular mechanisms mediating mammalian MEK-MAP Kinase cell survival signals. Cell Growth and Differentiation, 2001, 12:397-408. Abstract

B.A. Ballif, A. Shimamura, E. Pae and J. Blenis. Disruption of 3-Phosphoinositide Dependent Kinase 1 (PDK1) signaling by the anti-tumorigenic and anti-proliferative agent N-alpha-tosyl-L-phenylalanyl Chloromethyl Ketone. Journal of Biological Chemistry, 2001, 276:12466-12475. Abstract

A. Shimamura*, B.A. Ballif*, S.A. Richards and J. Blenis. RSK1 mediates a MEK-MAPK cell survival signal. Current Biology, 2000, 10:127-135. *Equal Contribution Abstract

B.A. Ballif, N.V. Mincek, J.T. Barret, M.L. Wilson and D.L. Simmons. Interaction of Cyclooxygenases-1 and -2 with an apoptosis and autoimmunity-associated protein. Proceedings of the National Academy of Sciences, USA, 1996, 93:5544-5549. Abstract