1. I need to treat my cell culture plates with volatile chemicals. Should I use a Biological Safety Cabinet (BSC) or a Fume Hood (FH) to do my work? - A Fume Hood will not protect your cells from contamination, and therefore, should not be used if you require aseptic conditions for your work (e.g., if you need to pass cells or continue an experiment in which cells must be placed back in the incubator). You could use the Fume Hood for a terminal procedure, such as RNA isolation with Trizol.