Work with biohazardous materials involving the use of volatile chemicals and/or radioactivity should exclusively be conducted in a biological safety cabinet that is vented outside the building.
Ducted Class II Type A2, Class II Type B1 and B2 (always hard-ducted), and Class III BSCs may be used with different amounts of chemicals/radioactive materials. Please review the following table for more specific details:
| BSC CLASS / TYPE | VOLATILE CHEMICALS | RADIONUCLIDES |
| II / A2 | If ducted outside building: minute amounts | If ducted outside building: trace amounts |
| II / B1 | Low levels | Trace amounts |
| II / B2 | Yes | Yes |
| III | Yes | Yes |