University of Vermont

The University of Vermont Cancer Center

Bio for Girja Shukla, Ph.D.
Girja Shukla, Ph.D.

Girja Shukla, Ph.D.

Associate Professor
Department of Surgery


Contact Information
E-mail: Girja.Shukla@uvm.edu
Office Location:
Given Bldg E303A, Burlington, VT 05405, USA

Education

M.S., Biochemistry, Lucknow University (1974)
Ph.D., Biochemistry, Lucknow University (1981)

Research Interests

Major interest is to develop targeted therapy against breast cancer. It involves in vitro (fresh tumors, cancer cell lines, purified cancer targets) and in vivo (animals and cancer patients) selection of tumor targeting agents using phage-displayed peptide, single chain antibody and ß-lactamase loop libraries. In addition, I am also interested in generating cancer-specific full-length human antibodies, using EBV immortalization and hybridoma techniques, from B cells obtained from sentinel nodes, bone marrow aspirates and tumor tissues of cancer patients

Academic Appointments

Associate Professor, Department of Surgery, University of Vermont College of Medicine, Burlington, VT  2002-Present  
Principal Scientist-EII, Indian Institute of Toxicology Research, Lucknow, UP  1998-2002   
Visiting Scientist (Sabbatical), Biochemistry Department, University of Vermont College of Medicine, Burlington, VT  1997-1999   
Principal Scientist-EI, Indian Institute of Toxicology Research, Lucknow, UP  1993-1998  
Visiting Scientist (Sabbatical), Mental Health Research Institute, University of Michigan, Ann Arbor, MI  1988-1990  
Senior Scientist-C, Indian Institute of Toxicology Research, Lucknow, UP  1988-1993  
Visiting Scientist (Sabbatical), Pharmacology Department, Ottawa University, Ottawa, ON  1983-1984  
Scientist-B, Indian Institute of Toxicology Research, Lucknow, UP  1983-1988  
Visiting Scientist (Sabbatical), Drug & Material Toxicology Department, University of Tennessee, Memphis, TN  1982-1983
Scientist-A, Indian Institute of Toxicology Research (a National Institute of CSIR), Lucknow, UP, India  1981-1983 

Awards and Honors

Top Reviewer Award (2010) by Elsevier press, Environmental Toxicology & Pharmacology publisher
Top Reviewer Award (2008) by Elsevier press, Environmental Toxicology & Pharmacology publisher
National Young Scientist Award,  (1989) Council of Scientific and Industrial Research, Government of India.

Publications

Shukla GS, Krag DN, Peletskaya EN, Pero SC, Sun YJ, Carman CL, McCahill LE, Roland TA.Intravenous infusion of phage-displayed antibody library in human cancer patients: enrichment and cancer-specificity of tumor-homing phage-antibodies. Cancer Immunol Immunother. 2013;62(8):1397-410.

Sun Y, Shukla G, Pero SC, Currier E, Sholler G, Krag D. Single tumor imaging with multiple antibodies targeting different antigens. Biotechniques. 2012;0(0):1-3.

Ambaye ND, Pero SC, Gunzburg MJ, Yap M, Clayton DJ, Del Borgo MP, Perlmutter P, Aguilar MI, Shukla GS, Peletskaya E, Cookson MM, Krag DN, Wilce MC, Wilce JA. Structural basis of binding by cyclic nonphosphorylated peptide antagonists of Grb7 implicated in breast cancer progression. J Mol Biol. 2011;412(3):397-411.

Shukla GS and Krag DN. Cancer cell-specific internalizing ligands from phage displayed ß-lactamase-peptide fusion libraries. Protein Engineering, Protein Eng Des Sel. 2010;23(6):431-40.

Shukla GS and Krag DN. Novel ß-lactamase-random peptide fusion libraries for phage display selection of cancer cell-targeting agents suitable for enzyme prodrug therapy. J Drug Target. 2010;18(2):115-24.

Shukla GS and Krag DN. Phage-displayed combinatorial peptide libraries in fusion to ß-lactamase as reporter for an accelerated clone screening: Potential uses of selected enzyme-linked affinity reagents in downstream applications. Comb Chem High Throughput Screen. 2010;13(1):75-87.

Shukla GS and Krag DN  Developing bifunctional ß-lactamase molecules with built-in target-recognizing module for prodrug therapy: Identification of Enterobacter cloacae P99 cephalosporinase loops suitable for randomization and phage-display selection. J Mol Recognit. 2009;22(6):425-36.

Shukla GS. Phage Display. In:  Encyclopedia of Cancer, second edition, 2009 (ISBN 978-3-540-47648-1), Edited by Manfred Schwab, Springer Press, Heidelberg, Germany.

Shukla GS and Krag DN. Selective delivery of therapeutic agents in diagnosis and treatment of cancer. Expert Opin Biol Ther. 2006;6(1):39-54.