DNA Analysis Facility
Senior Research Technician:
Mary Lou Shane
Lab Research Technician:
Placing an Order
- Please see the AGTC homepage for information on accessing services in the DNA Analysis Facility
- Bio-Rad Imagers (PDF)
- Fragment Analysis (PDF)
- Human Cell Line Authentication (PDF)
- New Bulk Sequence Service (PDF)
- Nucleic Acid Prep (PDF)
- PCR Array (PDF)
- Phred (PDF)
- Realtime qPCR (PDF)
- Sample, Submission, Storage & Retrieval Policy (PDF)
- Sequence (PDF)
- Sequence Email Message Explanations (PDF)
Health Science Research Facility
149 Beaumont Avenue
Burlington, VT 05405
Phone: (802) 656-2557
Fax: (802) 656-2140
The DNA Analysis Facility at the Vermont Cancer Center provides a wide array of efficient, user friendly, and affordable DNA analysis services to members of the Vermont Cancer Center, the University of Vermont research community, and outside institutions. Services provided for a fee include DNA Sequencing, RTqPCR, DNA fragment Analysis, nucleic acid extraction, image analysis, SNP detection, human cell line authentication and projects on demand.
The facility provides a variety of important benefits free of charge to our users including experimental design consultation, data analysis and trouble shooting. Our annual Open House is a popular day long event highlighting our services and new technologies. The facility hosts a monthly User Education Seminar series, presents software and instrumentation tutorials with the goal to educate and support our users, and showcase new services. A computer workstation area is adjacent to the main lab and has freeware and purchased programs available for user driven data analysis with full support from nearby facility staff.
- Automated DNA Sequencing
Cycle sequence reactions
- Fragment Analysis.
Genotyping, haplotyping, microsatellite markers
- Human Cell Line Authentication
Includes DNA extraction and microsatellite analysis
- Realtime qPCR
Quantitative PCR (absolute or relative)
Allelic Discrimination Assays
SNP Detection (low or high-throughput)
- RNA and DNA extractions
- Nucleic Acid and Protein Quantification
- Molecular Imaging
- Consultation and Troubleshooting
- Computer workstation with DNA analysis software
- ABI Prism 3100-Avant Genetic Analyzer (4 capillary)
- ABI Prism 3130xl Genetic Analyzer (16 capillary)
- ABI Prism 7900HT Sequence Detection System (Real-time qPCR)
- AB 7500 Fast Real-time PCR System
- BioRad Personal Molecular Imager FX
- BioRad PharosFX Molecular Imager FX (with external Laser)
- BioRad Versa Doc 4000 MP
- BioTek Synergy H4 Hybrid Reader
- DNA Engine 96-well gradient Thermal Cycler (2)
- NanoDrop (2)
The software accessible to users includes: GeneMapper, Autoassembler, Sequence Navigator, DNASTAR, Sequencher, Chromas, Edit View, BioEdit, Quantity One, Primer Express, Primer Select, and Dissociation Curve software.
Cancer Qualified Research:
- All Research Supported by the NCI with the following prefixes: R01, R03, R18, R25, R29, R35, R37, R55, P01, P50, U01, U10 and N01 research contracts and peer-reviewed, funded subcontracts of Center Members participating in collaborative research.
- Cancer-related Research Supported by Other NIH Institutes (same prefixes as above) and Funding Organizations (i.e. ACS (Nat'l), NSF, EPA, VA (Central Office), AICR, NIOSH, FDA, AHCPR, AFAR, DOD Breast Cancer Program, Susan G. Koman Breast Cancer Fd., Cancer Research Fd. Of America, & Howard Hughes Medical Institute). Grants and research contracts from other funding organizations may be eligible; contact Tim Hunter at 6-2559 regarding qualification procedure.
- Unfunded Clinical Research Studies approved by the NCI's Cancer Therapeutics Evaluation Program (CTEP) or the VCC Protocol Review Committee.
- VCC/CCSG or VCC/LCCRO approved Pilot Project Awards (e.g. LCCRO Award)
Citing the Facility
Please remember to cite the VCC DNA Analysis Facility in your abstracts and publications. These citations demonstrate to the NCI and other funding organizations (as well as the UVM administration) the importance of this facility to UVM researchers and thereby encourage their continued support. We try to keep a current list of all publications that cite the facility, so please let us know as when your papers are published.
For Cancer Qualifying projects that were completed prior to Nov. 30, 2008:
- "The automated DNA sequencing (or other molecular analyses, i.e. rtQPCR, MSI, etc.) was performed in the VT Cancer Center DNA Analysis Facility and was supported in part by grant P30CA22435 from the NCI. The views expressed are those of the author and do not represent the views of the NCI."
For Cancer Qualifying projects that were completed after Nov. 30, 2008:
- "The automated DNA sequencing (or other molecular analyses, i.e. rtQPCR, MSI, etc.) was performed in the VT Cancer Center DNA Analysis Facility and was supported by Vermont Cancer Center, Lake Champlain Cancer Research Organization, and the UVM College of Medicine."
For other users, state that DNA sequencing (or other molecular analysis) was performed in the VT Cancer Center DNA Analysis Facility.
Links of Interest
- Association of Biomolecular Research Facilities
- DNA Sequencing Research Group and Studies
- Fragment Analysis Research Group and Studies
- Microarray Research Group and Studies
- DNA Sequencing Research Group
- NCBI Homepage
- Journal of Biomolecular Techniques
- Beginners Guide to Molecular Biology
- Molecular Biology Freeware
Last modified May 30 2013 09:16 AM