Advanced Genome Technologies Core Facilities
The Advanced Genome Technologies Core is an overarching umbrella encompassing three distinct shared resource facility arms:
- DNA Analysis: The University of Vermont Cancer Center DNA Analysis Facility provides an array of fast, affordable, user-friendly DNA/RNA analysis services to the members of the University of Vermont Cancer Center, the University of Vermont research community and outside Institutions. Primary services offered for a fee include DNA Sanger sequencing, real-time quantitative PCR, DNA fragment analysis, SNP detection, nucleic acid extraction, image analysis, analytical flow cytometry, and support for absorbance, chemiluminescence, and fluorescence assays.
- Microarray: The Vermont Genetics Network Microarray Facility utilizes the Affymetrix GeneChip system to support global gene expression profiling using the standard 3’ arrays, miRNA, gene and exon arrays. The facility also provides supports for DNA idenfitification, quantification, and profiling for DNA studies using the DNA mapping (SNP), Promoter, Phylochip, and custom arrays.
- Massively Parallel Sequencing Facilities: The University of Vermont Cancer Center/College of Medicine Massively Parallel Sequencing Facility provides genome-scale next generation DNA sequencing. This facility employs the use of the massively paralleled sequencing (MPS) technology on the Illumina HiSeq 1000 system which is a sequencing by synthesis approach (SBS). Next generation sequencing (also known as deep sequencing or high throughput sequencing) enables researchers to sequence as much as an entire human or mouse genome with greater than 50 fold coverage. A single run on the Illumina HiSeq 1000 can generate over 350 billion bases of DNA/RNA.
The Advanced Genome Technologies Core is now using iLab Solutions for all service requests and distribution of data.
To get started, you must register for an account:
- Navigate to the core page.
- In the upper-right-hand corner of the screen, select sign up.
- Complete the registration form on the sign-up page.
- Receive a “welcome” email from iLab (typically within one business day) with login credentials
and basic instructions.
We have developed a number of “How to” Manuals to help with some basic functions:
- Internal UVM- How to register and set up your iLab account
- External to UVM- How to register and set up your iLab account
- How to Reserve Time on an Instrument
- How to Request a Service
- How to Generate a Project Request-Quote
- How to log use when using the BioRad Imagers
- Reporting Function in iLab
The Common Equipment Room is located in HSRF 307 and allows 24/7 access to a variety of user driven equipment for trained investigators. Equipment includes:
- Imaging equipment including: Bio-Rad Pharos FX PLUS and Versa Doc 4000 MP
- Beckman Coulter Analytical Flow Cytometer
- BioTek Synergy H4 Multi-plate reader
- NanoDrop Spectrophotometer
Design teams for both Microarray and Massively Parallel Sequencing have been assembled that include staff from the respective facilities plus bioinformaticians from the Molecular Bioinformatics Shared Resource and the Vermont Genetics Network Bioinformatics Core. All design team members maintain dedicated time weekly to provide this support.
Last modified August 26 2015 11:05 AM