University of Vermont

The University of Vermont Cancer Center

agtc.html

Advanced Genome Technologies Core Facilities

The University of Vermont Cancer Center

The Advanced Genome Technologies Core is an overarching umbrella encompassing three distinct shared resource facility arms: The University of Vermont Cancer Center DNA Analysis, the Vermont Genetic Network Microarray, and the University of Vermont Cancer Center-College of Medicine Massively Parallel Sequencing Facilities. The mission of the core is to comprehensively support research for investigators both by providing services to individual research laboratories as well as by playing a central role in bringing emerging technologies or approaches that are important for research investigators. The facilities offer a wide breadth of services to facilitate research involving identification, quantification, and profiling of nucleic acids.

Added Value Benefits to Core Users at No Additional Costs

The facility provides a variety of other important benefits free of charge to our users. These include access to two NanoDrop Spectrophotometers, Qubit Fluorometer, Bead Beater homogneizer and two MJ Research gradient thermal cyclers. The facility sponsors seminars on new technologies, holds tutorials on software and instrumentation use, and obtains discounted pricing on common reagents. Data analysis, troubleshooting and consultation in experimental design are provided free of charge to all users of the facility.  Our annual Open House is a popular day-long event highlighting all of our services.  The facility presents a monthly AGTC User Educational Core Seminar Series with the goal to educate and promote services currently being provided in the facility.  A computer workstation area is adjacent to the main lab and has freeware and purchased programs available for data analysis and review with full support from nearby facility staff.

How to Access Services

The Advanced Genome Technologies Core is now using iLab Solutions for all service requests and distribution of data.

To get started, you must register for an account:

  1. Navigate to the core page: https://my.ilabsolutions.com/service_center/show_external/3129.
  2. In the upper-right-hand corner of the screen, select sign up.
  3. Complete the registration form on the sign-up page: https://my.ilabsolutions.com/account/signup/322?sc_id=3129.
  4. Receive a “welcome” email from iLab (typically within one business day) with login credentials
    and basic instructions.

We have developed a number of “How to” Manuals to help with some basic functions:

Common Equipment Room:  307 HSRF

The common Equipment room was configured to allow access 24/7 to a variety of user driven equipment for trained investigators. Equipment that can be accessed here include:

  • Imaging equipment including:  Bio-Rad Pharos FX PLUS and Versa Doc 4000 MP
  • Beckman Coulter Analytical Flow Cytometer
  • 7500 FAST Real Time qPCR
  • BioTek Synergy H4 Multi-plate reader
  • NanoDrop Spectrophotometer

Experimental Design Consultations are Mandatory for all Microarray and Massively Parallel Sequencing Projects

Design teams for both Microarray and Massively Parallel Sequencing have been assembled that include staff from the respective facilities plus bioinformaticians from the Molecular Bioinformatics Shared Resource and the Vermont Genetics Network Bioinformatics Core.  All design team members maintain dedicated time weekly to provide this support.

Staff

AGTC Staff

Tim Hunter, Director, AGTC, timothy.hunter@uvm.edu, 656-2559
Scott Tighe, MPS, scott.tighe@uvm.edu, 656-AGTC
Mary Lou Shane, DNA, mary.shane@uvm.edu, 656-2557
Jessica Hoffman, DNA, Jessica.hoffman@uvm.edu, 656-2557
Meghann Palermo, Microarray, meghann.palermo@uvm.edu, 656-3936

Last modified December 05 2014 11:18 AM