University of Vermont

College of Medicine

Department of Pharmacology

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Totman Medical Research Trust

Vision

A premier research center on brain blood vessels, the Totman Medical Research Trust supports an interdepartmental, highly interactive research group focused on key aspects of brain artery function from molecules to clinical applications.

Participants

Trustees

Ms. Arlene Howard - Malone, NY

Mr. Donald Stone Jr. - Vienna, VA

Mr. Jeffrey Stone - Waterford, NY

 


Advisory Board

W. Jonathan Lederer, M.D., Ph.D. Professor and Director, Medical Biotechnology Center, University of Maryland; Visiting Professor, Department of Pharmacology, University of Vermont

Robert W. Hamill, M.D.
Professor, Department of Neurological Sciences

Bruce I. Tranmer, M.D.
Professor, Division of Neurosurgery, Department of Surgery; Program Director of Neurosurgery Residency Institutional Leadership

Fredrick C. Morin III, M.D.
Dean, College of Medicine

Russell Tracy, Ph.D.
Interim Senior Associate Dean for Research and Academic Affairs, College of Medicine; Professor, Department of Pathology

Brian Cote
Associate Dean for Finance and Administration, College of Medicine

 


 

Faculty

Mark T. Nelson, Ph.D. Professor and Chair, Department of Pharmacology; Professor, Department of Molecular Physiology & Biophysics

George C. Wellman, Ph.D. Associate Professor, Departments of Pharmacologyand Surgery (Division of Neurosurgery)

Joseph E. Brayden, Ph.D. Professor, Department of Pharmacology

Marilyn J. Cipolla, Ph.D. Professor, Departments of Neurology, Obstetrics & Gynecology, and Pharmacology

Wolfgang R. Dostmann, Ph.D. Professor, Departments of Pharmacology and Molecular Physiology & Biophysics

Karen M. Lounsbury, Ph. D. Professor, Department of Pharmacology

Adrian D. Bonev, Ph.D. Research Assistant Professor, Department of Pharmacology

Thomas Heppner, Ph.D. Research Assistant Professor, Department of Pharmacology

David Hill-Eubanks, Ph.D. Research Assistant Professor, Department of Pharmacology

Fabrice Dabertrand, Ph.D. Research Assistant Professor, Department of Pharmacology

Masayo Koide, Ph.D. Research Associate, Department of Pharmacology

Swapnil Sonkusare, Ph.D. Research Associate, Department of Pharmacology

 


 

Research Technical Support

Jessica Pearson
Senior Research Technician, Nelson Laboratory

Sheila Russell
Researcher/Analyst, Department of Surgery

Julie Sweet
Research Technician, Cipolla Laboratory

Matthew Tavares
Research Technician, Brayden Laboratory

Terry Wellman
Senior Research Technician, Lounsbury and Erdos Laboratories

 


Post-Doctoral Trainees

Thomas Dalsgaard, Ph.D.D.
Postdoctoral Associate, Nelson Laboratory

Katie Dunn, Ph.D.
Postdoctoral Associate, Nelson Laboratory

Albert Gonzales, Ph.D.
Postdoctoral Fellow, Nelson Laboratory

Thomas Longden, Ph.D.
Postdoctoral Fellow, Nelson Laboratory

Thomas Moon, Ph.D.
Postdoctoral Associate, Dostmann Laboratory

Nuria Villalba Isabel, Ph.D.
Postdoctoral Associate, Wellman/Freeman Laboratories

 


 

2013 Totman Lecturers

Luis Fernando Santana, Ph.D.
Professor, Department of Physiology and Biophysics, University of Washington, Seattle, WA

Kurt Beam, Ph.D.
Professor of Physiology and Biophysics, University of Colorado, Denver, Anschutz Medical Campus, Aurora, CO

PUBLICATIONS

Mark T. Nelson, Ph.D.

1. *Nausch B, Rode F, Jorgensen S, Nardi A, Korsgaard MPG, Hougaard C, Bonev AD, Brown WD, Dyhring T, Strobeak D, Olesen SP, Christohersen P, Grunnet M, Nelson MT, Ronn LCB. NS19504 A Novel BK channel activator with relaxing effect on bladder smooth muscle spontaneous phasic contractions. J Pharmacology Exp Ther. 2014 June 20. doi 10.1124/jpet.113.212662 PMID: 24951278

2. *Sonkusare SK, Dalsgaard T, Bonev AD, Hill-Eubanks DC, Kotlikoff MI, Scott JD, Santana LF, Nelson MT. AKAP150-dependent cooperative TRPV4 channel gating is central to endothelium-dependent vascodilation and is disrupted in hypertension. Scienc Signaling - in press.

3. *Hill-Eubanks DC, Gonzales AL, Sonkusare SK, Nelson MT. Vascular TRP Channels: Performing Under Pressure and Going with the Flow.  Physiology Review - in press.

4. *Gonzales AL, Yang Y, Sullivan MN, Sanders L, Dabertrand F, Hill-Eubanks DC, Nelson MT, Earley S.  A PLCy1-Dependent, Force-Sensitive Signaling Network in the Myogenic Constriction of Cerebral Arteries.   Science Signaling. 2014 May 27.  doi: 10.1126/scisignal.2004732.  PMID 24866019

5. *Longden TA, Dabertrand F, Hill-Eubanks DC, Hammack SE, Nelson MT.  Stress-induced glucocorticoid signaling remodels neurovascular coupling through impairment of cerebrovascular inwardly rectifying K+ channel function. Proc Natl Acad Sci USA. 2014 May 7. doi: 10.1073/pnas.1401811111.  PMID:  24808139, PubMed Central PMCID: PMC4034203

6. *Mercado J, Baylie R, Navedo MF, Yuan C, Scott JD, Nelson MT, Brayden JE, Santana LF.  Local control of TRPV4 channels by AKAP150-targeted PKC in arterial smooth muscle.   J Gen Physiol. 2014 May. 143(5): 559-575. doi: 10.1085/jgp.201311050. PMID: 24778429, PMCID: PMC4003184

7. *Krishnamoorthy G, Sokusare SK, Heppner TJ, Nelson MT.  Opposing roles of smooth muscle BK channels and ryanodine receptors in the regulation of nerve-evokeed constriction of mesenteric resistance arteries. Am J Physiol Heart Circ Physiol. 2014 Feb 7 (Epub ahead of print).  PMID: 24508642, PMCID: PMC3962538

8. *Dunn KM, Nelson MT.  Neurovascular signaling in the brain and the pathological consequences of hypertension. Am J Physiol Heart Circ Physiol. 2013 Oct 25. PMID: 24163077, PMCID: PMC3920149

 


George C. Wellman, Ph.D.

1. *Koide M, Syed AU, Braas KM, May V, Wellman GC. Pituitary adenylate cyclase activating polypeptide (PACAP) dilates cerebellar arteries through activation of large-conductance Ca2+ - activated (BK) and ATP-sensitive (KATP) K+ channels. J Mol Neurosci. (Epub ahead of print).

2. *Neill LA, Saundry CM, Hyman HN, Wellman GC. Evidence against a systemic arterial defect in patients with inflammatory bowel disease. J Surgical Research. 2014 April 16. pii: S0022-4804(14)000387-4. PMID: 24819742

3. *Koide M, Wellman GC. SAH-induced MMP activation and Kv current suppression is mediated via both ROS-dependent and ROS-independent mechanisms. Acta Neurchir Suppl - in press.

4. *Koide M, Wellman GC.  Activation of TRPV4 channels does not mediate inversion of neurovascular coupling after SAH.   Acta Neurochir Suppl - in press.

5. *Koide M, Sukhotinsky I, Ayata C, Wellman GC. Subarachnoid hemorrhage, spreading depolarizations and impaired neurovascular coupling. Stroke Treatment and Research. 2013: 819340. PMID: 23577279

6. *Wellman GC, Koide M.  Impact of Subarachnoid Hemorrhage on Parenchymal Arteriolar Function. Acta Neurochir Suppl. 2013; 115: 173-177.  PMID 22890666

7. *Koide M, Wellman GC.  SAH-induced suppression of voltage-gated K+ (Kv) channel currents in parenchymal arteriolar myocytes involves activation of the HB-EGF/EGFR pathway. Acta Neurchir Suppl. 2013; 115: 179-184. PMID: 22890665

8. *Koide M, Bonev AD, Nelson MT, Wellman GC.  Subarachnoid blood converts neurally evoked vasodilation to vasoconstriction in rat brain cortex. Acta Neurchir Suppl. 2013: 115: 167-171. PMID: 22890664

 


Joseph E. Brayden, Ph.D.

1. *Li Y, Baylie R, Brayden J. Involvement of TRPM4 channels in purinergic receptor-mediated myogenic tone development in cerebral parenchymal arterioles. J Cereb Blood Flow Metab. - under review.

2. *Li Y, Baylie R, Tavares M, Brayden J. Contribution Rho kinase to TRPM4-mediated myogenic tone in cerebral parenchymal arteriols. FASEB J. (abstract)

 


Marilyn J. Cipolla, Ph.D.

1. *Chan SL, Sweet JG, Cipolla MJ. Treatment for small vessel disease: Impact of relaxin on the function and structure of cerebral parenchymal arterioles. FASEB J. 2013 Oct; 27(10): 3917-3927. doi: 10.1096/fj.13-230797. PMID: 23783073, PMCID: PMC4046185

2. *Cipolla MJ, Sweet JG, Gokina N, White S, Nelson MT. Mechanisms of enhanced basal tone of brain parenchymal arterioles during early postischemic reprofusion: Role of ET-1 induced peroxynitrate generation. J Cerebral Blood Flow Metab. 2013 Oct; 33(10): 1486-1492. doi: 10.1038/jcbfm.2013.99. PMID: 23778163, PMCID: PMC3790940

3. *Cipolla MJ, Sweet JG, Chan SL, Tavares MJ, Gokina N and Brayden JE. Increased pressure-induced tone in brain parenchymal arterioles vs. middle cerebral arteries in rats: Role of ion channels and calcium sensitivity. J Appl Physiol. 2014 Jul 1; 117(1): 53-59. doi: 10.1152/japlphysiol.00253.2014. PMID: 24790017, PMCID: PMC4101583

4. *Wallace K, Tremble SM, Owens MY, Morris R, Cipolla MJ.  Plasma from HELLP syndrome patients increases blood-brain barrier permeability. Repro Sci. 2014 (Under revision.)

5. *Cipolla MJ, Chan SL, Sweet, JG, Tavares MJ, Gokina N, Brayden JE.  Postischemic reprofusion causes smooth muscle calcium sensitation and vasoconstriction of parenchymal arterioles. Stroke. 2014 (Submitted.)

Book Chapters:

1. *Cipolla MJ, Zeeman GG and Cunningham G. Cerebrovascular Pathophysiology in Preeclampsia and Eclampsia. In: Hypertensive Disorders in Pregnancy, 4th Ed., Cunningham, Roberts and Taylor, Eds. 2014

Invited Reviews:

1. *Morales Palomares S, Cipolla MJ. Invited Review: Myogenic tone as a thereapeutic target for ischemic stroke. Curr Vasc Pharm. 2014 - in press.

2. *Cipolla MJ. The adaptation of the cerebral circulation to pregnancy: Mechanisms and conseqences. J Cerebr Blood Flow Metab. 2013. 33(4): 465-478. PMID: 23321787, PMCID: PMC3618397

 


Wolfgang R. Dostmann, Ph.D.

1. *Moon TM, Osborne BW, Dostmann WR.  The switch helix: A putative combinatorial relay for interprotomer communication in cGMP-dependent protein kinase. Biochim Biophys Acta. 2013 1834: 1346-1351. PMID: 23416533, PMCID: PMC3683381

4. *Held KF, Dostmann WR. Real-time monitoring the spatiotemporal dynamics of intracellular cGMP in vascular smooth muscle cells. Methods Mol Bio. 2013, 1020: 131-145. PMID: 23709030

 


Karen M. Lounsbury, Ph.D.

1. *Williams TF, Mirando AT, WIlkinson B, Francklyn CS, Lounsbury KM. Secreted Threonyl-tRNA synthetase stimulates endothelial cell migration and angiogenesis. Nature Sci Repts. 2013 3:1317. PMID: 23425968, PMCID: PMC3578223

2. *Wellman TF, Williams TF, Wong C, Francklyn CS, Lounsbury KM.  Threonyl-tRNA Synthetase Overexpression Correlates with Angiogenesis and Progression of Human Ovarian Cancer. 2014 (in press)

 


 

Kalev Freeman, M.D.

1. *Watts R, Thomas A, Filipi CG, Nickerson JP, Freeman K. Potholes and molehills: Bias in diagnostic performance of diffusion tensor imaging in concussion. Radiology. (2014, Mar epub ahead of print).  PMID:  24635677

2. *Thomas A, Watts R, Filippi CG, Nickerson JP, Andrews T, Lieberman G, Naylor MR, Eppstein MJ, Freeman K. Longitudinal magnetic resonance diffusion tensor imaging improves diagnostic performance for mild traumatic brain injury. Radiology. (2014, submitted).

3: *Villalba N, Sonkusare SK, Longden TA, Tran TL, Nelson MT, Wellman GC, Freeman K. Traumatic brain injury disrupts cerebrovascular tone through endothielial iNOS expression and nitric oxide gain-of-function. Circulation Research. (2014, submitted). 

*Totman Medical Research Fund Acknowledged

Last modified August 21 2014 09:47 AM