University of Vermont

College of Medicine

Department of Pharmacology

nelsonlab

Nelson Lab

Mark T. Nelson, Ph.D

University Distinguished Professor and Chair
Department of Pharmacology
Goggle Scholar Citations Profile

Contact Information

Department of Pharmacology
The University of Vermont, College of Medicine
Given Building, Room B-333A
89 Beaumont Avenue
Burlington, VT 05405-0068

Email: mark.nelson@uvm.edu
Phone: (802) 656-2500
Fax: (802) 656-4523

Research Interests

The overall goal of the research in Dr. Nelson's laboratory is to understand the control of smooth muscle and endothelial cell function by ion channels and calcium signaling.

There are three major research areas in the lab:

  1. To understand the mechanisms by which computationally active neurons in the brain control local cerebral blood flow (CBF) ("neurovascular coupling"), using optical techniques to measure calcium signaling and arteriolar diameter in the neurovascular unit (neurons, astrocytes, arteriolar smooth muscle and endothelium) in brain slices as well as CBF in vivo, electrophysiological techniques to measure membrane currents and membrane potential of astrocytes, smooth muscle and endothelial cells from parenchymal arterioles. Arteriolar diameter is also measured in isolated pressurized parenchymal arterioles.
  2. To understand how sympathetic nerves, smooth muscle cells and endothelial cells communicate ("vascular crosstalk") to control the function of resistance-sized peripheral arteries.
  3. To understand the roles of ion channels and calcium signaling in the control of urinary bladder function in health and disease.

Approaches cover the spectrum from molecular, cellular, intact tissue, whole organ and in vivo (local CBF, blood pressure, urodynamics). A number of genetic mouse models are used to unravel control mechanisms. Relevant ion channels in smooth muscle, endothelium and astrocytes are being explored, including voltage-dependent calcium channels, inward rectifier potassium channels, calcium-sensitive BK, IK, SK channels, voltage-dependent potassium channels, ATP-sensitive potassium channels, TRPV4 channels, ryanodine receptor channels, IP3R channels, and P2X1 receptor channels. The ultimate objections are to understand the basic mechanisms for ion channel control of local cerebral blood flow, peripheral resistance and urinary bladder function, and using this information to understand pathologies and possible new therapeutic interventions.

People in the Lab

Faculty and Research Scientists

Faculty and Research Scientists - Dr. Adrian Bonev, Dr. Fabrice Dabertrand, Dr. Thomas Heppner, Dr. David Hill-Eubanks, Dr. Nathan Tykocki

 

Postdoctoral Associates and Fellows

Majid Ahmed, Ph.D., Daniel Collier, Ph.D., Albert Gonzales, Ph.D., Osama Harraz, Ph.D., and Johanna Schleifenbaum, Ph.D.

 

Laboratory Research Technicians

Technicians Samantha O'Dwyer and Jessica Pearson

Recent Publications

For a complete list of Mark T. Nelson's publications, please visit PubMed.

  1. Mann KG, Freeman K, Esmond CT, Wisnewski S, Tracy RP, Kindzelski AL, Pusateri A, Banerjee A, Brass LF, Brummel-Ziedins KE, Butenas S, Cohen MJ, Diamond SL, Moore EE, Morrissey JL, Nelson MT, Park MS, Ruf W, Shupp JW, Spearry JL, Spiess BD, Stalker TJ, Zuckerbraun BS. TACTIC: Trans-Agency Consortium for Trauma-Induced Coagulopathy. J Thromb Haemost. 2015 Jun; 13 Suppl 1: S63-S71. doi: 10.1111/jth.12981. PMID: 26149052, PMCID: PMC4498273
  2. Balbi M, Ghosh M, Longden TA, Jativa Vega M, Gesierich B, Hellal F, Lourbopoulos A, Nelson MT, Plesnila N. Dysfunction of mouse cerebral arteries during early aging. J Cereb Blood Flow Metab. 2015 Jun 10. doi: 10.1038/jcbfm.2015.107. PMID: 26048694
  3. Collier DM, Hill-Eubanks DC, Nelson MT. Orchestrating Ca2+ influx through CaV1.2 and CaV3.x channels in human cerebral arteries. J Gen Physiol. 2015 Jun; 145(6): 481-483. doi: 10.1085/jgp.2015.11411. PMID: 2609543, PMCID: PMC 4442783
  4. Joutel A, Haddad I, Ratelade J, Nelson MT. Perturbations of the cerebrovascular matrisome: a convergent mechanism in small vessel disease of the brain? J Cereb Blood Flow Metab. 2015 Apr 8. doi: 10.1038/jcbfm.2015.62. PMID: 25853907
  5. Dabertrand F, Kroigaard C, Bonev AD, Cognat E, Dalsgaard T, Domenga-Denier V, Hill-Eubanks DC, Brayden JE, Joutel A, Nelson MT. Potassium channelopathy-like defect underlies early-stage cerebrovascular dysfunction in a genetic model of small vessel disease. Proc Natl Acad Sci USA. 2015 Feb 17: 112(7): E796-E805. doi: 10.1073/pnas.1420765112. PMID: 25646445, PMCID: PMC4343103
  6. Longden TA, Nelson MT. Vascular Inward Rectifier K+ Channels as External K+ Sensors in the Control of Cerebral Blood Flow. Microcirculation. 2015 Jan 31; doi: 10.1111/micc.12190. PMID: 25641345, PMCID: PMC4404517
  7. Villalba N, Sonkusare SK, Longden TA, Tran TL, Sackheim AM, Nelson MT, Wellman GC, Freeman K. Traumatic brain injury disrupts cerebrovascular tone through endothelial inducible nitric oxide synthase expression and nitric oxide gain of function. J Am Heart Assoc, 2014 Dec 19; 3(6), pii: e001474. doi: 10.1161/JAHA.114.001474. PMID: 25527626, PMCID: PMC4338739
  8. Longden TA, Hammack SE, Nelson MT. Channeling Stress: inwardly-rectifying K+ channels in stress and disease, Channels (Austin). 2014: 8(4): 296-297. PMID: 25478622
  9. Hill-Eubanks DC, Gonzales AL, Sonkusare SK, Nelson MT. Vascular TRP Channels: Performing Under Pressure and Going with the Flow. Physiology Review. 2014 Sept; 29(5): 343-360. doi: 10.1152/physiol.00009.2014. PMID: 25180264, PMCID: PMC4214829
  10. Mingin GC, Peterson A, Erickson CS, Nelson MT, Vizzard MA. Social stress induces changes in urinary bladder function, bladder NGF content and generalized bladder inflammation in mice. Am J Physiol Regul Integr Comp Physiol. 2014 Aug 6. pii: ajpregu.00500.2013. E pub ahead of print. PMID: 25100077, PMCID: PMC4187184
  11. Sonkusare SK, Dalsgaard T, Bonev AD, Hill-Eubanks DC, Kotlikoff MI, Scott JD, Santana LF, Nelson MT. AKAP150-dependent cooperative TRPV4 channel gating is central to endothelium-dependent vasodilation and is dirupted in hypertension. Science Signaling. 2014 Jul 8. doi: 10.1126/scisignal.2005052. Journal cover. PMID: 25005230, PMCID: 4403000, with Perspective: Pooneh B, Garland CJ. Scaffolding Builds to Reduce Blood Pressure, Science Signaling. 2014 July 8; 7(333) pe16. doi: 10.1126/scisignal.2005527
  12. Nausch B, Rode F, Jorgensen S, Nardi A, Korsgaard MP, Hougaard C, Brown WD, Bonev AD, Dyhring T, Strobeak D, Olesen SP, Christophersen P, Grunnet M, Nelson MT, ROnn LC. NS19504: a novel BK channel activator with relaxing effect on bladder smooth muscle spontaneous phasi contractions. J Pharmacol Exp Ther. 2014 Jun 20. doi: 10.1124/jpet.113.212662. PMID: 24951278, PMCID: PMC4152884
  13. Gonzales AL, Yang Y, Sullivan MN, Sanders MN, Sanders L, Dabertrand F, Hill-Eubanks DC, Nelson MT, Earley S. A PLCy1-Dependent, Force-Sensitive Signaling Network in the Myogenic Constriction of Cerebral Arteries. Science Signaling. 2014 May 27. doi: 10.1126/scisignal.2004732. PMID: 24866019, PMCID: PMC4170587
  14. Longden TA, Dabertrand F, Hill-Eubanks DC, Hammack SE, Nelson MT. Stress-induced glucocorticoid signaling remodels neurovascular coupling through impairment of cerebrovascular inwardly rectivying K+ channel function; Proc Natl Acad Sci USA. 2014 May 7. doi: 10.1073/pnas.1401811111. PMID: 24808139, PMCID: PMC4034203
  15. Mercardo J, Baylie R, Navedo MF, Yuan C, Scott JD, Nelson MT, Brayden JE, Santana LF. Local control of TRPV4 channels by AKAP150-targeted PKC in arterial smooth muscle. J Gen Physiol. 2014 May; 143(5): 559-575. doi: 10.1085/jgp.201311050. PMID: 24778429, PMCID: PMC4003184
  16. Krishnamoorthy G, Sonkusare SK, Heppner TJ, Nelson MT.  Opposing roles of smooth muscle BK channels and ryanodine receptors in the regulation of nerve-evoked constriction of mesenteric resistance arteries. Am J Physiol Heart Circ Physiol.  2014 Feb 7.  doi: 10.1152/ajpheart.00866.2013. PMID:  24508642, PMCID: PMC3962638
  17. Dunn KM, Nelson MT.  Neurovascular signaling in the brain and the pathological consequences of hypertension. Am J Physiol Heart Circ Physiol.  2014 Jan1. doi: 10.1152/ajpheart.00364.2013.  PMID:  24163077, PMCID: PMC3920149
  18. Cipolla MJ, Sweet JG, Gokina N, White S, Nelson MT. (2013): Mechanisms of enhanced basal tone of brain parenchymal arterioles during early postischemic reperfusion: Role of ET-1 induced peroxynitrite generation. J Cerebr Blood Flow Metab.  2013 Jun 19.  doi:  10.1038/jcbfm.2013.99.   PMID:  23778163, PMCID: PMC3790940
  19. Dunn KM, Hill-Eubanks DC, Liedtke WB, Nelson MT.  TRPV4 channels stimulate Ca2+ induced Ca2+ release in astrocytic endfeet and amplify neurovascular coupling responses.   Proc Natl Acad Sci USA.  2013 Apr 9; 110(15):  6157-6162.  doi:  10.1073/pnas.1216514110.  PMID:  23530219, PMCID: PMC3625327 
  20. Dabertrand F, Hannah RM, Pearson JM, Hill-Eubanks DC, Brayden JE, Nelson MT. Prostaglandin E(2), a postulated astrocyte-derived neurovascular coupling agent, constricts rather than dilates parenchymal arterioles.  J Cereb Blood Flow Metab.  2013 Feb 6.  doi: 10.1038/jcbfm.2013.9.  PMID:  23385200, PMCID: PMC3618402

Honors and Awards

2015 Thomas C. Vary Distinguished Lecture, University of Pennsylvania, Hershey, PA
2015 Keynote Speaker, Society of General Physiologists Symposium on Macromolecular Local Signaling Complexes, Marine Biological Laboratory, Woods Hole
2015 American Physiological Society, Annual Reviews Award for Scientific Reviewing
2015 Kaley Lecture, Experimental Biology 2015, Boston MA, sponsored by APS Cardiovascular Section and the Microcirculation Society
2013 Keynote Speaker, 6th International Conference on cGMP, Erfurt, Germany
2012 Society of General Physiologists Traveling Scholar Award, Harvard University
2012 15th Annual James W. Fisher Distinguished Lectureship in Pharmacology, Tulane University, New Orleans, LA
2012 Keynote Speaker, FASEB Smooth Muscle Conference, Snowmass Village, CO
2011 Astor Lecturer, University of Oxford, Department of Pharmacology
2009 Swift Memorial Lecture, Columbia University, Department of Physiology and Cellular Biophysics
2009 Lamport Lecture, University of Washington, Department of Physiology and Biophysics
2009- University Distinguished Professor, University of Vermont
2009- Fellow, The Biophysical Society
2009 5th David F. Bohr Lecture on Vascular Smooth Muscle, 10th International Symposium on Mechanisms of Vasodilation
2008 Graduate Student's Choice Speaker of the Year, Department of Pharmacology & Toxicology, University of Arkansas for Medical Sciences, Little Rock, AR
2008 Graduate Student's Choice Speaker of the Year, Student Research Day, University of New Mexico School of Medicine Biomedical Sciences Graduate Program, Albuquerque, NM
2006 Distinguished Lecturer, North West Universities of England; The Universities of Liverpool and Manchester
1998- Member, Vermont Academy of Sciences and Engineering
1996 University Scholar, University of Vermont
1985-1990 Established Investigator, American Heart Association

Funding

National Institutes of Health
7/1/14- 6/30/19

1R01HL121706-01A1, Regulation of myoendothelial function by signaling microdomains in hypertension, PI M.T. Nelson
National Institutes of Health
8/1/2010 - 7/31/2015
P01 HL09548801, Calcium signaling in the cerebrovascular unit in health and disease, PI M.T. Nelson, Project 1 and Administrative Core. 
National Institutes of Health
8/1/1998-5/31/2018
R37 DK53832, Ca2+ sparks and urinary bladder smooth muscle excitability, PI: M.T. Nelson.

National Institutes of Health
9/30/13-6/30/2018

UM1HL120877, Analysis and Characterization of Trauma-Induced Coagulopathy, PI K.G. Mann, M.T. Nelson Lead Project 12 and Co-I Project 1
Totman Medical Research Trust
8/22/1989-Present
PI: M.T. Nelson.
Fondation Leducq
10/1/2012-9/30/2017
Pathogenesis of Small Vessel Disease of the Brain. North American Coordinator, M.T. Nelson; European Coordinator, A. Joutel (Paris).

Last modified August 03 2015 04:09 PM