Research in Dr. Lounsbury's laboratory is focused on understanding mechanisms whereby signals generated at the plasma membrane are communicated to the nucleus to mediate vascular remodeling and tumor angiogenesis. Signaling pathways of current interest include protein kinase cascades, hypoxia signaling and mechanisms of calcium-regulated gene transcription in arterial smooth muscle cellsand ovarian tumor cells. The laboratory uses a multidisciplined approach to study these pathways using techniques from biochemistry, molecular biology, and cell biology.
The model systems are vascular smooth muscle cell and ovarian cancer cell cultures as well as intact arteries, in situ tumors and human samples. The techniques used in these projects include cell culture, RNA isolation, quantitative RT-PCR, Western blotting, cell imaging, arterial dissection and tumor analysis. The results of these studies will give a better understanding of how vascular and tumor cells respond to their environment and whether transcriptional changes are subject to therapeutic intervention.
Ongoing Research Projects:
Regulation of angiogenesis by hypoxia in cancer cells and vascular smooth muscle cells
Calcium-regulated gene transcription in vascular smooth muscle cells
Last modified March 19 2014 08:38 AM