1987 - PhD, Biochemistry, Oklahoma State University, Stillwater, OK1981 - BA, Math/Chemistry, State University of New York at Potsdam
Our overall research is geared towards better understanding the role of circulating biomarkers for human disease. All too often we look for one answer to very broad disease states. Terms such as cardiovascular disease, cancer, and aging are very broad terms for what has turned out to be very complicated diseases involving genetics, environment and viral or bacterial challenges. Each individual has a different contribution from each area, thus having a unique biomarker profile. If we can understand an individual profile for disease, we can provide better targeted medical interventions.
Endothelial Progenitor Cells and circulating endothelial cells in COPD: We are currently measuring endothelial progenitor cells and circulating endothelial cells in a case control study of chronic obstructive pulmonary disease. Circulating endothelial progenitor cells are thought to be a measure of vascularization, while circulating endothelial cells are thought to be a measure of endothelial breakdown. We are looking for differences in each of these measures and the two taken together as an indication of progression of chronic obstructive pulmonary disease.
The role of innate and adaptive immune cells in atherosclerosis: We have determined cellular levels of a variety of immune cells (T cells, T helper cells, Th1, Th2, T cells, natural killer cells, memory T helper cells, naïve T helper cells, endothelial progenitor cells and resting and stimulated monocyte tissue factor levels) from ~1000 people of various ethnic background. These participants have been followed for 10 years with various information collected, including cardiovascular, anthropometric, nutrition and exercise, as well as blood, urine and cellular components. We have demonstrated that circulating Th1 levels show a positive correlation to the coronary artery calcium as well as intima media thickness. The Th1 level appears to be driven, to a large part, by the titer of CMV in the participant. The exact mechanism for this remains to be elucidated. The role of these immune cells in other disease states in currently under investigation. We are also attempting to find plasma markers for these cells that will allow estimation of cell types from stored plasma samples.
The nature of soluble cellular receptors circulating in blood: As soluble receptors found circulating in the blood appear to play a role in disease, we set forth to determine the exact nature of these soluble receptors. Of current interest are the soluble Il6 receptor, the soluble CD14 receptor and the soluble IL2 receptor alpha. These receptor may exist in three forms: a full length form that remains associated with lipid as a microparticle circulating through blood, an ectodomain form that has been cleaved from the cell by a “sheddase”, or an alternatively spliced soluble form that is released from cellular granules. We are using gel filtration column chromatography, SDS gel electrophoresis and western blooting techniques to determine which type or types exist in plasma, if different types exist in different people, and if different types exist between disease states.
2004-present Research Associate, Department of Pathology, University of Vermont
2002-2004 Senior Researcher, University of Vermont, Burlington, Vermont
1992-1994 Senior Scientist, Haemtalogic Technologies Inc., Essex Junction, Vermont
1987-1990 Postdoctoral Associate, Department of Biochemistry, University of Vermont
1987-1990 Recipient, National Heart, Lung and Blood Institute Training Grant, University of Vermont
2013 Olson NC, Sallam R, Doyle MF, Tracy RP, Huber SA.T helper cell polarization in healthy people: implications for cardiovascular disease. J Cardiovasc Transl Res. 2013 Oct;6(5):772-86. PMID: 23921946 [PubMed - in process] 2013 Olson NC, Doyle MF, Jenny NS, Huber SA, Psaty BM, Kronmal RA, Tracy RP. Decreased naive and increased memory CD4(+) T cells are associated with subclinical atherosclerosis: the multi-ethnic study of atherosclerosis. PLoS One. 2013 Aug 23;8(8):e71498.PMID: 24009662 [PubMed - in process]
2013 Tracy RP, Doyle MF, Olson NC, Huber SA, Jenny NS, Sallam R, Psaty BM, Kronmal RA. T-helper type 1 bias in healthy people is associated with cytomegalovirus serology and atherosclerosis: the multi-ethnic study of atherosclerosis. J Am Heart Assoc. 2013 May 20;2(3):e000117. PMID: 23688675 [PubMed - in process]
2013 Thomashow MA, Shimbo D, Parikh MA, Hoffman EA, Vogel-Claussen J, Hueper K, Fu J, Liu CY, Bluemke DA, Ventetuolo CE, Doyle MF, Barr RG. Endothelial microparticles in mild chronic obstructive pulmonary disease and emphysema. The Multi-Ethnic Study of Atherosclerosis Chronic Obstructive Pulmonary Disease study. Am J Respir Crit Care Med. 2013 Jul 1;188(1):60-8. PMID: 23600492 [PubMed - indexed for MEDLINE] For a complete list of Margaret Doyle's publications, please visit PubMed.