University of Vermont

College of Medicine

Microscopy Imaging Center

University of Vermont College of Medicine Microscopy Imaging Center Transmission Electron Microscope

Transmission Electron Microscope

Transmission Electron Microscope

The JEOL 1400 Transmission Electron Microscope is a high-contrast, high-resolution instrument utilizing a LaB6 filament, and capable of operating at accelerating voltages up to 120 kV. It incorporates a newly designed electron optics system which is optimized for integrated use with a digital ccd camera imaging device. The instrument is controlled via the “TEM Center” graphical user interface operating environment, with redundant manual operation afforded by two conveniently located control panels. The image orientation system allows image rotation at multiple angles, and specimen stage movement is achieved with a precision trackball device. Software modules (JEOL Electron Microscope Navigation Interactive Engine: “JENIE”) provide step-by-step video tutorials covering instrument operation and alignment, especially useful for novice operators. Image acquisition provided by an AMT XR611 high resolution 11 megapixel mid-mount ccd camera. This camera is positioned near the normal film plane of the TEM affording a wide field of view for image capture. Moreover, the mid-mount configuration displays virtually no TEM distortion.

TEM slide
TEM slide
Papers published from the MIC:

Hou X, Lewis KT, Wu Q, Wang S, Chen X, Flack A, Mao G, Taatjes DJ, Sun F, Jena BP. (2014) Proteome of the porosome complex in human airway epithelia: interaction with the cystic fibrosis transmembrane conductance regulator (CFTR). J Proteomics. Jan 16;96:82-91.

Kovari LC, Brunzelle JS, Lewis KT, Cho WJ, Lee JS, Taatjes DJ, Jena BP. (2014) X-ray solution structure of the native neuronal porosome-synaptic vesicle complex: Implication in neurotransmitter release. Micron. Jan;56:37-43.

Hatle KM, Gummadidala P, Navasa N, Bernardo E, Dodge J, Silverstrim B, Fortner K, Burg E, Suratt BT, Hammer J, Radermacher M, Taatjes DJ, Thornton T, Anguita J, Rincon M. (2013) MCJ/DnaJC15, an endogenous mitochondrial repressor of the respiratory chain that controls metabolic alterations. Mol Cell Biol. Jun;33(11):2302-14.

Cunniff B, Benson K, Stumpff J, Newick K, Held P, Taatjes D, Joseph J, Kalyanaraman B, Heintz NH. (2013) Mitochondrial-targeted nitroxides disrupt mitochondrial architecture and inhibit expression of peroxiredoxin 3 and FOXM1 in malignant mesothelioma cells. J Cell Physiol. Apr;228(4):835-45.

Lee JS, Hou X, Bishop N, Wang S, Flack A, Cho WJ, Chen X, Mao G, Taatjes DJ, Sun F, Zhang K, Jena BP. (2013) Aquaporin-assisted and ER-mediated mitochondrial fission: a hypothesis.  Micron. Apr;47:50-8.  

Wang S, Lee JS, Bishop N, Jeremic A, Cho WJ, Chen X, Mao G, Taatjes DJ, Jena BP. (2012) 3D organization and function of the cell: Golgi budding and vesicle biogenesis to docking at the porosome complex.  Histochem Cell Biol. Jun;137(6):703-18.  

Hillegass JM, Blumen SR, Cheng K, MacPherson MB, Alexeeva V, Lathrop SA, Beuschel SL, Steinbacher JL, Butnor KJ, Ramos-Nino ME, Shukla A, James TA, Weiss DJ, Taatjes DJ, Pass HI, Carbone M, Landry CC, Mossman BT. (2011) Increased efficacy of doxorubicin delivered in multifunctional microparticles for mesothlioma therapy. Int. J. Cancer. Jul;129(1):233-44.

Additional papers.

Last modified April 15 2014 01:01 PM