Transmission Electron Microscope
The JEOL 1400 Transmission Electron Microscope is a high-contrast, high-resolution instrument utilizing a LaB6 filament, and capable of operating at accelerating voltages up to 120 kV. It incorporates a newly designed electron optics system which is optimized for integrated use with a digital ccd camera imaging device. The instrument is controlled via the “TEM Center” graphical user interface operating environment, with redundant manual operation afforded by two conveniently located control panels. The image orientation system allows image rotation at multiple angles, and specimen stage movement is achieved with a precision trackball device. Software modules (JEOL Electron Microscope Navigation Interactive Engine: “JENIE”) provide step-by-step video tutorials covering instrument operation and alignment, especially useful for novice operators. Image acquisition provided by an AMT XR611 high resolution 11 megapixel mid-mount ccd camera. This camera is positioned near the normal film plane of the TEM affording a wide field of view for image capture. Moreover, the mid-mount configuration displays virtually no TEM distortion.
Papers published from the MIC:
MCJ/DnaJC15, an endogenous mitochondrial repressor of the respiratory chain that controls metabolic alterations. Hatle KM, Gummadidala P, Navasa N, Bernardo E, Dodge J, Silverstrim B, Fortner K, Burg E, Suratt BT, Hammer J, Radermacher M, Taatjes DJ, Thornton T, Anguita J, Rincon M. Mol Cell Biol. 2013 Jun;33(11):2302-14. doi: 10.1128/MCB.00189-13. Epub 2013 Mar 25. PMID: 23530063
Mitochondrial-targeted nitroxides disrupt mitochondrial architecture and inhibit expression of peroxiredoxin 3 and FOXM1 in malignant mesothelioma cells. Cunniff B, Benson K, Stumpff J, Newick K, Held P, Taatjes D, Joseph J, Kalyanaraman B, Heintz NH. J Cell Physiol. 2013 Apr;228(4):835-45. doi: 10.1002/jcp.24232. PMID: 23018647
Aquaporin-assisted and ER-mediated mitochondrial fission: a hypothesis. Lee JS, Hou X, Bishop N, Wang S, Flack A, Cho WJ, Chen X, Mao G, Taatjes DJ, Sun F, Zhang K, Jena BP. Micron. 2013 Apr;47:50-8. doi: 10.1016/j.micron.2013.01.005. Epub 2013 Jan 1. PMID: 23416165
3D organization and function of the cell: Golgi budding and vesicle biogenesis to docking at the porosome complex. Wang S, Lee JS, Bishop N, Jeremic A, Cho WJ, Chen X, Mao G, Taatjes DJ, Jena BP. Histochem Cell Biol. 2012 Jun;137(6):703-18. doi: 10.1007/s00418-012-0948-x. Epub 2012 Apr 13. PMID: 22527693
Hillegass JM, Blumen SR, Cheng K, MacPherson MB, Alexeeva V, Lathrop SA, Beuschel SL, Steinbacher JL, Butnor KJ, Ramos-Nino ME, Shukla A, James TA, Weiss DJ, Taatjes DJ, Pass HI, Carbone M, Landry CC, Mossman BT. (2010) Increase efficacy of doxorubicin delivered in multifunctional microparticles for mesothlioma therapy. Int. J. Cancer.
Last modified November 13 2013 02:16 PM