Laser Capture Microdissection
The Arcturus (Life Technologies) XT-Ti Laser Capture Microdissector system is used for specifically removing desired cells from a tissue section for subsequent molecular analysis. The Laser Capture Microdissector includes both gentle infrared cell capture and rapid ultraviolet laser cutting. Cells are captured automatically using an interactive pen-display monitor and trackball-actuated stage. The microdissector is integrated onto a Nikon Eclipse-Ti inverted microscope, equipped with 2X, 10X, and 40X objective lenses. Fluorescence illumination is accomplished with a Lumen Dynamics X-Cite lamp module with liquid guide. Fluorescence cubes for uv excitation (325-375nm Ex/420 Em), blue excitation (450-490nm Ex/515nm Em), green excitation (510-560 nm Ex/590nm Em), and red excitation (590-650nm Ex/663-738nm Em) provide the ability to microdissect and capture fluorescently labeled cells within tissues. A second ccd camera has been added to a side port on the microscope for high resolution acquisition of brightfield and fluorescence images. Tissue sections can be microdissected from plain glass slides, PEN membrane coated slides, and framed, coated slides.
Papers published from the MIC:
Manning CB, Sabo-Attwood T, Robledo RF, MacPherson MB, Rincon M, Vacek P, Hemenway D, Taatjes DJ, Lee PJ, Mossman BT. (2008) Targeting the MEK1 cascade in lung epithelium inhibits proliferation and fibrogenesis by asbestos. Am. J. Respir. Cell Mol. Biol. 38, 618-626.
Manning CB, Mossman BT, Taatjes DJ. (2006) Analysis of asbestos-induced gene expression changes in bronchiolar epithelial cells using laser capture microdissection and quantitative reverse-transcriptase polymerase chain reaction (QRT-PCR). Cell Imaging Techniques (DJ Taatjes and BT Mossman, eds) Methods in Molecular Biology, Vol. 319, Humana Press, Totowa, NJ, pp. 231-236.
Manning C, Cummins A, Jung M, Berlanger I, Timblin C, Palmer C, Taatjes DJ, Hemenway D, Vacek P, Mossman B. (2002) A mutant epidermal growth factor receptor targeted to lung epithelium inhibits asbestos-induced proliferation and aroto-oncogene expression. Cancer Research 62, 4169-4175.
Taatjes DJ, Palmer CJ, Pantano C, Buder-Hoffmann S,Cummins A, Mossman BT. (2001) Laser-based microscopic approaches: Application to cell signaling in environmental lung disease. BioTechniques 31:880-894.
Last modified November 13 2013 02:18 PM