Confocal Laser Microscopy
The Zeiss LSM 510 META confocal scanning laser microscope is a state-of-the-art multi-spectral imaging system, consisting of a confocal scanning system attached to an Axiovert 200M inverted light microscope. A combination of diode and argon lasers provide excitation lines of 405 nm, 458 nm, 477 nm, 488 nm, 514 nm, 543 nm and 633 nm. The system incorporates two conventional detectors with the META spectral detector, allowing for the visualiztion of up to eight fluorophores per specimen. An environmental chamber attached to the microscope stage provides for the control of temperature, gas and humidity for long duration live-cell experiments. Software packages for physiology experiments and 3-D reconstruction of image sets are also part of the system.
Papers published from the MIC:
MCJ/DnaJC15, an endogenous mitochondrial repressor of the respiratory chain that controls metabolic alterations. Hatle KM, Gummadidala P, Navasa N, Bernardo E, Dodge J, Silverstrim B, Fortner K, Burg E, Suratt BT, Hammer J, Radermacher M, Taatjes DJ, Thornton T, Anguita J, Rincon M. Mol Cell Biol. 2013 Jun;33(11):2302-14. doi: 10.1128/MCB.00189-13. Epub 2013 Mar 25. PMID: 23530063
Cell adhesion molecule 1 (CADM1) is ubiquitously present in the endothelium and smooth muscle cells of the human macro- and micro-vasculature. Tatsumi K, Taatjes DJ, Wadsworth MP, Bouchard BA, Bovill EG. Histochem Cell Biol. 2012 Nov;138(5):815-20. doi: 10.1007/s00418-012-1024-2. Epub 2012 Sep 1. PMID: 22940845
Last modified November 13 2013 02:17 PM