Confocal Laser Microscopy
The Zeiss LSM 510 META confocal scanning laser microscope is a state-of-the-art multi-spectral imaging system, consisting of a confocal scanning system attached to an Axiovert 200M inverted light microscope. A combination of diode and argon lasers provide excitation lines of 405 nm, 458 nm, 477 nm, 488 nm, 514 nm, 543 nm and 633 nm. The system incorporates two conventional detectors with the META spectral detector, allowing for the visualiztion of up to eight fluorophores per specimen. An environmental chamber attached to the microscope stage provides for the control of temperature, gas and humidity for long duration live-cell experiments. Software packages for physiology experiments and 3-D reconstruction of image sets are also part of the system.
Papers published from the MIC:
Klaus J, Eisenhauer P, Russo J, Mason A, Do D, King B, Taatjes D, Cornillez-Ty C, Boyson JE, Zheng C, Liao L, Yates JR III, Zhang B, Ballif BA, Botten J. (2013) The intracellular cargo receptor ERGIC-53 is required for the production of infectious arenavirus, coronavirus, and filovirus particles. Cell Host & Microbe. Nov;14: 522-534.
Hatle KM, Gummadidala P, Navasa N, Bernardo E, Dodge J, Silverstrim B, Fortner K, Burg E, Suratt BT, Hammer J, Radermacher M, Taatjes DJ, Thornton T, Anguita J, Rincon M. (2013) MCJ/DnaJC15, an endogenous mitochondrial repressor of the respiratory chain that controls metabolic alterations. Mol Cell Biol. Jun;33(11):2302-14.
Tatsumi K, Taatjes DJ, Wadsworth MP, Bouchard BA, Bovill EG. (2012) Cell adhesion molecule 1 (CADM1) is ubiquitously present in the endothelium and smooth muscle cells of the human macro- and micro-vasculature. Histochem Cell Biol. Nov;138(5):815-20.
Last modified April 07 2014 12:28 PM