University of Vermont

College of Medicine

Department of Medicine

Cardiovascular Medicine

Bio for Robert Kelm, Ph.D.
Robert Kelm, Ph.D.

Robert Kelm, Ph.D.

Associate Professor of Medicine
Associate Professor of Biochemistry


Contact Information
E-mail: Robert.Kelm@uvm.edu
Office Location:
Colchester Research Facility, Room 165

Expertise

My research program seeks to uncover the molecular mechanisms that promote the phenotypic reprogramming of disease- or injury-activated cell types of the heart, blood, and vasculature. Using a combination of biochemical, biophysical, and cell-based approaches, my research group has identified several structurally and functionally unique single-stranded nucleic acid-binding proteins, which repress the expression of genes required for the differentiation of muscle, stromal, and leukocyte cell types. We are currently investigating the molecular biology of the purine-rich element binding proteins A and B (Pura and Purb) and Y-box binding protein 1 (YB-1). The goal of our studies is to understand how these ssDNA/RNA-binding factors modulate the dysfunctional phenotypes adopted by vascular and myeloid cells in the context of arteriosclerosis and hematopoietic malignancy.

Publications

Representative Publications from a total of 42:

Rumora AE, Wang SX, Ferris LA, Everse SJ, Kelm RJ Jr (2013) Structural basis of multisite single-stranded DNA recognition and ACTA2 repression by purine-rich element binding protein B (Purß). Biochemistry 52(26):4439-50.

David JJ, Subramanian SV, Zhang A, Willis WL, Kelm RJ Jr, Leier CV, Strauch AR (2012) Y-box binding protein-1 implicated in translational control of fetal myocardial gene expression after cardiac transplant. Exp Biol Med (Maywood) 237(5):593-607.

Gurha P, Abreu-Goodger C, Wang T, Ramirez MO, Drumond AL, van Dongen S, Chen Y, Bartonicek N, Enright AJ, Lee B, Kelm RJ Jr, Reddy AK, Taffet GE, Bradley A, Wehrens XH, Entman ML, Rodriguez A (2012) Targeted deletion of microRNA-22 promotes stress-induced cardiac dilation and contractile dysfunction. Circulation 125(22):2751-61.

Rumora AE, Steere AN, Ramsey JE, Knapp AM, Ballif BA, Kelm RJ Jr (2010) Isolation and characterization of the core single-stranded DNA-binding domain of purine-rich element binding protein B (Purß). Biochem Biophys Res Commun 400(3):340-5.

French CJ, Zaman AK, Kelm RJ Jr, Spees JL, Sobel BE (2010) Vascular rhexis: loss of integrity of coronary vasculature in mice subjected to myocardial infarction. Exp Biol Med (Maywood) 235(8):966-73.

Kollander R, Solovey A, Milbauer LC, Abdulla F, Kelm RJ Jr, Hebbel RP (2010) Nuclear factor-kappa B (NFkappaB) component p50 in blood mononuclear cells regulates endothelial tissue factor expression in sickle transgenic mice: implications for the coagulopathy of sickle cell disease. Transl Res 155(4):170-7.

Hebbel RP, Vercellotti GM, Pace BS, Solovey AN, Kollander R, Abanonu CF, Nguyen J, Vineyard JV, Belcher JD, Abdulla F, Osifuye S, Eaton JW, Kelm RJ Jr, Slungaard A (2010) The HDAC inhibitors trichostatin A and suberoylanilide hydroxamic acid exhibit multiple modalities of benefit for the vascular pathobiology of sickle transgenic mice. Blood 115(12):2483-90.

van Rooij E, Quiat D, Johnson BA, Sutherland LB, Qi X, Richardson JA, Kelm RJ Jr, Olson EN (2009) A family of microRNAs encoded by myosin genes governs myosin expression and muscle performance. Dev Cell 17(5):662-73.

Ramsey JE, Kelm RJ Jr (2009) Mechanism of strand-specific smooth muscle a-actin enhancer interaction by purine-rich element binding protein B (Purß). Biochemistry 48(27):6348-60.

Zhang A, David JJ, Subramanian SV, Liu X, Fuerst MD, Zhao X, Leier CV, Orosz CG, Kelm RJ Jr, Strauch AR (2008) Serum response factor neutralizes Pur alpha- and Pur beta-mediated repression of the fetal vascular smooth muscle alpha-actin gene in stressed adult cardiomyocytes. Am J Physiol Cell Physiol 294(3):C702-14.

Knapp AM, Ramsey JE, Wang SX., Strauch AR, Kelm RJ Jr (2007) Structure-function analysis of mouse Pur beta II: Conformation altering mutations disrupt single-stranded DNA and protein interactions crucial to smooth muscle alpha-actin gene repression. J Biol Chem 282(49):35899-909.

Ramsey JE, Daugherty MA, Kelm RJ Jr (2007) Hydronamic studies on the quarternary structure of recombinant mouse Pur beta. J Biol Chem 282(3):1552-60.

Wang SX, Elder PK, Zheng Y, Strauch AR, and Kelm RJ Jr (2005) Cell cycle-mediated regulation of smooth muscle a-actin gene transcription in fibroblasts and vascular smooth muscle cells involves multiple adenovirus E1A-interacting cofactors. J Biol Chem 280:6204-14.

For a complete list of publications click here to go to Google Scholar.