University of Vermont

College of Medicine

Department of Biochemistry

Bio for Thomas Orfeo
Thomas Orfeo

Thomas Orfeo

Contact Information
Office Location:
Biochemistry, Colch Res Fac 248


Dr. Orfeo received his PhD in 1987 from the Cell and Molecular Biology Program at the University of Vermont. His graduate work involved investigating potential physiologic roles for classes of esterases present in liver and muscle. He then worked on projects at the University of Vermont involving endothelial cell regulation of coagulation and fibrinolysis in response to anoxia/reperfusion injury or traumatic force and the regulation of transcription in Acanthamoeba. He joined the laboratory of Dr. Kenneth G. Mann in 1999 and has since worked on a number of projects studying the enzymatic mechanisms and regulatory pathways of blood coagulation.


Orfeo T, Doherty JM, Adey G, Penar PL, Shatos MA. Sublethal percussion trauma in vitro causes a persisting derangement in the nonthrombogenic properties of brain endothelial cells. J. Trauma 1994; 37: 347-357.

Orfeo T, Brufatto N, Nesheim ME, Xu H, Butenas S, Mann KG. The factor V activation   paradox. J.Biol.Chem. 2004; 279: 19580-19591.

Butenas S, Orfeo T, Gissel  MT, Brummel Ziedins KE,  Mann KG. The significance of circulating IXa in blood.  J. Biol.Chem. 2004; 279:22875-22882.

Orfeo T, Butenas S,  Brummel-Ziedins KE, Mann KG. The tissue factor requirement in blood coagulation. J. Biol. Chem 2005; 280:42887-42896.

Orfeo T, Brummel-Ziedins KE, Gissel M, Butenas S, Mann KG. The nature of the stable blood clot procoagulant activities. J. Biol. Chem 2008; 283:9776-9786.

Danforth CM, Orfeo T, Mann KG, Brummel-Ziedins KE, Everse SJ. The impact of uncertainty in a blood coagulation model. Math. Med.Biol. 2009 (epub)

Foley JH, Orfeo T, Undas A, McLean KC, Bernstein IM, Rivard GE, Mann KG, Everse SJ, Brummel-Ziedins KE (2013) From principle to practice: bridging the gap in patient profiling. PLoS One 8(1): e54728.

Haynes LM, Orfeo T, Mann KG (2012) Rivaroxaban delivery and reversal at a venous flow rate. Arterioscler Thromb Vasc Biol 32(12): 2877-83.

Brummel-Ziedins KE, Orfeo T, Callas PW, Gissel M, Mann KG, Bovill EG (2012) The prothrombotic phenotypes in familial protein C deficiency are differentiated by computational modeling of thrombin generation. PLoS One 7(9): e44378.

Whelihan MF, Zachary V, Orfeo T, Mann KG (2012) Prothrombin activation in blood coagulation: the erythrocyte contribution to thrombin generation. Blood 120(18): 3837-45.

Gissel M, Orfeo T, Foley JH, Butenas S (2012) Effect of BAX499 aptamer on tissue factor pathway inhibitor function and thrombin generation in models of hemophilia. Thromb Res 130(6): 948-55.

Bravo MC, Orfeo T, Mann KG, Everse SJ (2012) Modeling of human factor Va inactivation by activated protein C. BMC Syst Biol 6: 45.

Danforth CM, Orfeo T, Everse SJ, Mann KG, Brummel-Ziedins KE (2012) Defining the boundaries of normal thrombin generation: investigations into hemostasis. PLoS One 7(2): e30385.