Dr. Morrical received his Ph.D. in Biochemistry from the University of Wisconsin-Madison in 1987. His postdoctoral research at the University of California-San Francisco focused on mechanisms of recombination-dependent DNA synthesis and repair. He joined the UVM Biochemistry faculty in 1992. Dr. Morrical is also an adjunct member of the MMG department and a member of the Vermont Cancer Center. His research is funded by grants from the NIH and National Cancer Institute.
Ma, Y., Wang, T., Villemain, J.L., Giedroc, D.P., Morrical, S.W. (2004). Dual functions of single-stranded DNA-binding protein in helicase loading at the bacteriophage T4 DNA replication fork. J Biol Chem., 279(18):19035-19045.
All Morrical Publications
Bleuit, J.S., Ma, Y., Munro, J., Morrical, S.W. (2004). Mutations in a conserved motif inhibit single-stranded DNA binding and recombination mediator activities of bacteriophage T4 UvsY protein. J Biol Chem., 279(7):6077-6086.
Bleuit, J.S., Xu, H., Ma, Y., Wang ,T., Liu, J., Morrical, S.W. (2001). Mediator proteins orchestrate enzyme-ssDNA assembly during T4 recombination-dependent DNA replication and repair. Proc Natl Acad Sci USA., 98(15):8298-305.
Villemain, J.L., Ma, Y., Giedroc, D.P., Morrical, S.W. (2000). Mutations in the N-terminal cooperativity domain of gene 32 protein alter properties of the T4 DNA replication and recombination systems. J Biol Chem., 275(40):31496-504.
Xu, H., Wang, Y., Bleuit, J.S., Morrical, S.W. (2001). Helicase assembly protein Gp59 of bacteriophage T4: fluorescence anisotropy and sedimentation studies of complexes formed with derivatives of Gp32, the phage ssDNA binding protein. Biochemistry, 40(25):7651-6.
Beernink, H.T.H. & Morrical, S.W. (1999). RMPs: Recombination/replication mediator proteins. Trends Biochem. Sci., 24: 385-389.
Ando, R.A. & Morrical, S.W. (1999). Relationship between hexamerization and ssDNA binding affinity in the uvsY recombinationprotein of bacteriophage T4. Biochemistry, 38:16589-16598. Farb JN, Morrical SW. Functional complementation of UvsX and UvsY mutations in the mediation of T4 homologous recombination. Nucleic Acids Res. 2009 Apr;37(7):2336-45. Epub 2009 Feb 25.
Farb JN, Morrical SW. Role of allosteric switch residue histidine 195 in maintaining active-site asymmetry in presynaptic filaments of bacteriophage T4 UvsX recombinase. J Mol Biol. 2009 Jan 16;385(2):393-404. Epub 2008 Nov 12.
Pant K, Shokri L, Karpel RL, Morrical SW, Williams MC. Modulation of T4 gene 32 protein DNA binding activity by the recombination mediator protein UvsY. J Mol Biol. 2008 Jul 25;380(5):799-811. Epub 2008 May 24.
Liu J, Qian N, Morrical SW. Dynamics of bacteriophage T4 presynaptic filament assembly from extrinsic fluorescence measurements of Gp32-single-stranded DNA interactions. J Biol Chem. 2006 Sep 8;281(36):26308-19. Epub 2006 Jul 7.
Liu J, Bond JP, Morrical SW. Mechanism of presynaptic filament stabilization by the bacteriophage T4 UvsY recombination mediator protein. Biochemistry. 2006 May 2;45(17):5493-502.
Maher RL, Morrical SW (2013) Coordinated Binding of Single-Stranded and Double-Stranded DNA by UvsX Recombinase. PLoS One 8(6): e66654.
Branagan AM, Maher RL, Morrical SW (2012) Assembly and dynamics of Gp59-Gp32-single-stranded DNA (ssDNA), a DNA helicase loading complex required for recombination-dependent replication in bacteriophage T4. J Biol Chem 287(23): 19070-81.
Maher RL, Branagan AM, Morrical SW (2011) Coordination of DNA replication and recombination activities in the maintenance of genome stability. J Cell Biochem 112(10): 2672-82.
Liu J, Ehmsen KT, Heyer WD, Morrical SW (2011) Presynaptic filament dynamics in homologous recombination and DNA repair. Crit Rev Biochem Mol Biol 46(3): 240-70.
Liu J, Morrical SW (2010) Assembly and dynamics of the bacteriophage T4 homologous recombination machinery. Virol J 7: 357.
Chen J, Villanueva N, Rould MA, Morrical SW (2010) Insights into the mechanism of Rad51 recombinase from the structure and properties of a filament interface mutant. Nucleic Acids Res 38(14): 4889-906.
Xu H, Beernink HT, Morrical SW (2010) DNA-binding properties of T4 UvsY recombination mediator protein: polynucleotide wrapping promotes high-affinity binding to single-stranded DNA. Nucleic Acids Res 38(14): 4821-33.
Branagan, A., Maher, R., and Morrical, S. Assembly and Dynamics of Gp59-Gp32-Single-stranded DNA (ssDNA), a DNA Helicase Loading Complex Required for Recombination-dependent Replication in Bacteriophage T4. J Biol Chem. 2012 Jun 1;287(23):19070-81. Epub 2012 Apr 12.
Maher, R., Branagan, A., Morrical, S. Coordination of DNA replication and recombination activities in the maintenance of genome stability. J Cell Biochem. 2011 Jun 6. doi: 10.1002/jcb.23211. [Epub ahead of print]