Bio for Stephen Everse Stephen Everse Contact Information E-mail: Stephen.Everse@uvm.eduOffice Location: Biochemistry, Given B418A EducationPh.D. 1995 University of California, San Diego, La Jolla, California (Chemistry) Thesis title: Insights into the structure of human fibrinogen. Advisor: Dr. Russell F. Doolittle B.S. 1988 Beloit College, Beloit, Wisconsin (Chemistry)ExpertiseDr. Everse received his Ph.D. in Chemistry from the University of California, San Diego in 1995. His postdoctoral work at UCSD focused on obtaining a structural understanding of the fibrinogen molecule. His work resulted in the X-ray crystallographic structures of the fibrinogen fragment D and the fibrin fragment double-D. He joined the Biochemistry Department in the Fall of 1998 as part of the HHMI Structural Biology Initiative at UVM. Academic Appointments1998- present Assistant Professor, Dept. of Biochemistry, College of Medicine, University of Vermont, Burlington, Vermont1997- 1998 Postdoctoral Fellow in the lab of Dr. R.F. Doolittle, Deptartment of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California 1990- 1992 Teaching Assistant, Department of Chemistry, University of California, San Diego, La Jolla, California 1995- 1997 American Heart Association, California Affiliate Postdoctoral Fellow in the lab of Dr. R.F. Doolittle, Deptartment of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California1988- 1989 Student Researcher in the lab of Dr. J. Visser, Department of Genetics, Landbouwuniversiteit, Wageningen, The NetherlandsAwards and HonorsHonors Group of the Westinghouse National Science Talent Search (1984) Project: Why snakes molt? The isolation of snake skin peroxidase. Beloit College Presidential Scholarship (1984-1988)Walter S. Haven Fellowship (1987, 1988) Project: Restriction mapping and BENZER Edward Stevens Research Award (1988) Project: A physical chemistry laboratory using singlet oxygen. Edward C. Fuller Chemical Education Award (1988) Outstanding teaching assistant, organic chemistry laboratory. Carl Welty Honor Award in Biology (1988) Greatest service to the Department of Biology. Martha Peterson Senior Class Award (1988) Most outstanding senior class scholar and campus wide activist. Outstanding Teaching Award, Dept. of Chemistry UCSD (1990)Honorable Mention, Outstanding Teaching Award, Dept. of Chemistry UCSD (1992)American Heart Association, California Affiliate Postdoctoral Fellowship (1995 - 1997)Co-Editor of The BioQUEST (Biology Quality Undergraduate Educational Simulations and Tools) Library (1996 - current)Co-Leader, BioInformatics Workshop, Bangkok, Thailand (6-10 January 1997, 17-21 January 2000 & 6-10 January 2003) American Society of Hematology/Wyeth-Ayerst Scholar (2000-2002)PublicationsG. Spraggon, D. Applegate, S.J. Everse, J-Z. Zhang, L. Veerapandian, C. Redman, R. F. Doolittle, and G. Grieninger. Crystal structure of a recombinant aEC domain from human fibrinogen-420. Proceedings of the National Academy of Science 95: 9099-9104, 1998.J. Zanocco, I. Caldicott, and S.J. Everse. Strobomicroscopy: A quantitative, noninvasive biophysics lab. Midwest Bioscene, 14(1): 24-30, 1988.L.O. Lutherer, S.J. Everse, and J.L. Williams. Neurons of the rostral fastigial nucleus are responsive to cardiovascular and respiratory challenges. Journal of the Autonomic Nervous System 27(2): 101-11, 1989.J.L. Williams, S.J. Everse, and L.O. Lutherer. Stimulating fastigial nucleus alters central mechanisms regulating phrenic activity. Respiratory Physiology 76: 215-228, 1989.S.S. Twining, S.J. Everse, P.M. Wilson, B.Y.J.T. Yue, and S.K. Chan. Localization of the a-1-proteinase inhibitor in the human cornea. Current Eye Research 8(4): 389-95, 1989.S. J. Everse, H. Pelletier, and R. F. Doolittle. Crystallization of fragment D from human fibrinogen. Protein Science 4: 1013-1016, 1995.M.O. Skidmore, M.R. Sawaya, S. Parkin, B. Rupp, H. Hope, S.J. Everse, and G.W. Walter. Crystallization of the Aa subunit of the protein phosphatase 2A. Protein Science 5: 1198-1200, 1996.R.F. Doolittle, S.J. Everse, and G. Spraggon. Human fibrinogen: anticipating a 3-dimensional structure. FASEB Journal 10: 1464-1470, 1996.R.F. Doolittle, G. Spraggon, and S.J. Everse. Evolution of vertebrate fibrin formation and the process of its dissolution. 1997 Plasminogen-related Growth Factors (CIBA Foundation Symposium 212). Wiley: Chichester, p. 4-23.G. Spraggon*, S.J. Everse*, and R.F. Doolittle. Crystal structures of fragment D from human fibrinogen and its crosslinked counterpart from human fibrin. Nature 389: 455-462, 1997. (*= these authors contributed equally)S.J. Everse, G. Spraggon, and R.F. Doolittle. A three-dimensional consideration of variant human fibrinogens. Thrombosis and Haemostatsis 80:1-9, 1998.S.J. Everse*, G. Spraggon*, L. Veerpandian, M. Riley, and R.F. Doolittle. Crystal structure of fragment double-D from human fibrin with two different bound ligands. Biochemistry 37: 8637-8642, 1998. (*= these authors contributed equally)J.R. Jungck, V. Streif, I. Caceraj, and S.J. Everse. BENZER: An interval graph tool for deletion mapping, restriction mapping and food webs. BioQUEST Library Volume IV, 1998, Academic Press, Inc.: San Diego.R.F. Doolittle, G. Spraggon, and S.J. Everse. Three-dimensional structural studies on fragments of fibrinogen and fibrin. Current Opinion in Structural Biology 8(6): 792-8, 1998.J.R. Jungck, V. Vaughan, J.N. Calley, S.J. Everse, P. Soderberg, E. Stanley, and J. Stewart. BioQUEST Library Volume V, 1999, Academic Press, Inc.: San Diego.S.J. Everse, G. Spraggon, L. Veerpandian, and R.F. Doolittle. Conformational changes in fragments D and double-D from human fibrin(ogen) upon binding the peptide ligand Gly-His-Arg-Pro-amide. Biochemistry, 38(10): 2941-2946, 1999.R.F. Doolittle, G. Spraggon, and S.J. Everse. Crystal structures of fragment D and double-D from fibrinogen and fibrin. Thrombosis and Haemostasis 82(2): 271-6, 1999.M.F. Hockin, K.C. Jones, S.J. Everse, and K.G. Mann. A model for the stoichiometric regulation of blood coagulation. Journal of Biological Chemistry. 277(21): 18322-33, 2002.19. A.B. Mason, Q-Y. He, P.J. Halbrooks, S.J. Everse, D.R. Gumerov, I.A. Kaltashov, V.C. Smith, J. Hewitt, and R.T. MacGillivray. Differential effect of a His tag at the N- and C-termini: functional studies with recombinant human serum transferrin. Biochemistry 41(30): 9448-54, 2002.Z. Yang, G. Spraggon, L. Pandi, S.J. Everse, M. Riley, and R.F. Doolittle RF. Crystal structure of fragment D from lamprey fibrinogen complexed with the peptide Gly-His-Arg-Pro-amide. Biochemistry 41(32): 10218-24, 2002.S.J. Everse. New insights into fibrin(ogen) structure and function, Vox Sanguinis 83 (Suppl. 1): 375-82, 2002.T.E. Adams, A.B. Mason, Q-Y. He, P.J. Halbrooks, S.K. Briggs, V.C. Smith, R.T.A. MacGillivray, and S.J. Everse. The position of arginine 124 controls the rate of iron release from the N-lobe of human serum transferrin: A structural study. Journal of Biological Chemistry, In press.T.E. Adams, S.J. Everse, and K.G. Mann. Predicting the Pharmacology of Thrombin Inhibitors. Journal of Thrombosis and Haemostasis, In press.P.J. Halbrooks, Q-Y. He, S.K. Briggs, S.J. Everse, V.C. Smith, R.T.A. MacGillivray, and A.B. Mason. Investigation of the mechanism of iron release from the C- lobe of human serum transferrin: Mutational analysis of the role of a pH sensitive triad, In press.All Everse Publications Everse SJ, Doublie S. (2006). Abstract Crystallographic software: a sustainable resource for the community. Methods Mol Biol., 364:273-8. Adams TE, Hockin MF, Mann KG, and Everse SJ. (2004). The crystal structure of activated protein C-inactivated bovine factor Va: Implications for cofactor function. Proc Natl Acad Sci USA,101(24):8918-23. Epub 2004 Jun 07 View the structure at PDB Halbrooks PJ, Mason AB, Adams TE, Briggs SK, Everse SJ. (2004). The oxalate effect on release of iron from human serum transferrin explained. J Mol Biol., 339(1):217-26. View the structure at PDB Adams TE, Everse SJ, Mann KG. (2003). Predicting the pharmacology of thrombin inhibitors. J Thromb Haemost.,1(5):1024-7. Halbrooks PJ, He QY, Briggs SK, Everse SJ, Smith VC, MacGillivray RT, and Mason AB. (2003). Investigation of the Mechanism of Iron Release from the C-Lobe of Human Serum Transferrin: Mutational Analysis of the Role of a pH Sensitive Triad. Biochemistry, 42(13):3701-7 Adams TE, Mason AB, He QY, Halbrooks PJ, Briggs SK, Smith VC, MacGillivray RT, and Everse SJ. (2003). The Position of Arginine 124 Controls the Rate of Iron Release from the N-lobe of Human Serum Transferrin. A STRUCTURAL STUDY. J Biol Chem., 278(8):6027-33. View the structure at PDB Yang Z, Spraggon G, Pandi L, Everse SJ, Riley M, Doolittle RF. (2002). Crystal structure of fragment D from lamprey fibrinogen complexed with the peptide Gly-His-Arg-Pro-amide. Biochemistry, 41(32):10218-24. View the structure at PDB Hockin MF, Jones KC, Everse SJ, Mann KG. (2002). A model for the stoichiometric regulation of blood coagulation. J Biol Chem., 277(21):18322-33. Everse SJ. (2002). New insights into fibrin (ogen) structure and function. Vox Sang., 83 Suppl 1:375-82.