Regulation of non-muscle myosins during cytokinesis and vesicle transport.
I received my BS in Biology from UMass Amherst. Shortly after graduation, I moved up to Wilder, VT where I worked as a research technician in Mako Saito’s Lab in the Genetics Department of Dartmouth Medical School. During the 3 years that I spent studying cell-cycle regulation with Dr. Saito, I completed my MS in Clinical Research at The Dartmouth Institute.
I decided to join the CMB PhD program at UVM after living in Burlington for a few years working in a variety of clinical healthcare and research positions. The vibrant academic community in Burlington is an exciting place to pursue my research interests, while the beautiful Green Mountains provides enough wilderness to fuel my outdoor passions.
My research is focused on the role of tropomyosins in regulating actomyosin interactions. Tropomyosins are a class of coiled-coil protein that bind along actin filaments. Eukaryotic organisms depend upon specific actomyosin interactions for diverse cellular functions, including: maintaining cortical integrity, providing intracellular transport, and generating the contractile force necessary for endocytosis and cytokinesis. Tropomyosin has been extensively studied in the context of muscle cells, where it regulates sarcomere contraction. However, over 40 isoforms of non-muscle tropomyosins have been identified in mammals. Although each class of myosin relies on the same actin substrate to generate force, specific actomyosin interactions occur at different actin structures within the cell. Recent studies have suggested that tropomyosins are important in regulating these interactions. Additionally, several tropomyosin isoforms have been implicated in the transformation of cancer cells. My work is currently aimed at comparing the ability of mammalian and fungal tropomyosins to regulate class II and class V myosins.
Roy SH, Clayton JE, Holmen J, Beltz E, & Saito RM (2011) Control of Cdc14 activity coordinates cell cycle and development in Caenorhabditis elegans. Mechanisms of development 128(7-10):317-326.
Sammons, M.R., James, M.L., Clayton, JE, Sladewski, T.E., Sirotkin, V., and Lord, M. (2011). A calmodulin-related light chain from fission yeast that functions with myosin-I and PI 4-kinase. Journal of cell science 124, 2466-2477.
Clayton JE, Sammons MR, Stark BC, Hodges AR, & Lord M (2010) Differential regulation of unconventional fission yeast myosins via the actin track. Current biology : CB 20(16):1423-1431.
Clayton JE, van den Heuvel SJ, & Saito RM (2008) Transcriptional control of cell-cycle quiescence during C. elegans development. Developmental biology 313(2):603-613.
Clayton JE, Pollard LW, Sckolnick M, Bookwalter CS, Hodges AR, Trybus KM, Lord M (2013) Fission yeast tropomyosin specifies directed transport of myosin-V along actin cables. Mol Biol Cell in press.
Sammons MR, James ML, Clayton JE, Sladewski TE, Sirotkin V, Lord M (2011) A calmodulin-related light chain from fission yeast that functions with myosin-I and PI 4-kinase. J Cell Sci 124(Pt 14): 2466-77.
Clayton JE, Sammons MR, Stark BC, Hodges AR, Lord M (2010) Differential regulation of unconventional fission yeast myosins via the actin track. Curr Biol 20(16): 1423-31.
Office: C141C Given
Lab: 140 HSRF
- 12/10/2013 11:30 AM – 12:30 PM
Dr. Kelly Fimlaid
- 12/17/2013 11:30 AM – 12:30 PM
- 1/28/2014 11:30 AM – 12:30 PM
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