Role of myosin motors in the cell.



Profile

BSc Microbiology, University of Sheffield, UK (1991-94); DPhil Biochemistry, University of Oxford, UK (1994-98); HF Long-term Fellow, Harvard Univ, USA (1998-2001); Post-doctoral Fellow, Yale University, USA (2001-05); Assistant Professor, Univ. Vermont (2006-2013);

Associate Professor, University of Vermont (since 2013)

Research Description

The long-term goal of our research lies in understanding how the actin-based myosin motors operate in the cell. Our lab focuses on the roles of myosin-I (in endocytosis), myosin-II (in cytokinesis), and myosin-V (in organelle and mRNA transport).

We employ both the fission and budding yeasts as models, taking advantage of the versatile molecular genetics and cell biology approaches on offer in these systems. Current interests lie in regulation of myosins by i) modification of the actin track, and ii) UCS domain proteins.

We utilize a variety of techniques including molecular biology, yeast genetics, and live cell imaging to track actomyosin dynamics (at contractile rings, endocytic patches, and during intracellular transport). We have optimized the purification of myosin-I, -II, and -V from yeast, allowing us to complement our in vivo approaches with biochemical assays to dissect mechanisms of regulation. Knowing how myosin motors are controlled in cells represents an important step in understanding the actomyosin-dependent processes governing cell proliferation and motility in cancer cells.

Highlighted Publications

Clayton JE, Pollard LW, Murray GG, Lord M. Myosin motor isoforms direct specification of actomyosin function by tropomyosins. Cytoskeleton (Hoboken). 2015 Feb 25. doi: 10.1002/cm.21213 PMID: 25712463

Pollard LW, Lord M. Getting myosin-V on the right track: tropomyosin sorts transport in yeast. Bioarchitecture. 2014 Jan-Feb;4(1):35-8

Clayton JE, Pollard LW, Sckolnick M, Bookwalter CS, Hodges AR, Trybus KM, Lord M. Fission yeast tropomyosin specifies directed transport of myosin-V along actin cables. Mol Biol Cell. 2014; 25(1):66-75; PMID: 24196839

Stark BC, James ML, Pollard LW, Sirotkin V, Lord M. UCS protein Rng3p is essential for myosin-II motor activity during cytokinesis in fission yeast. PLoS One 2013 8(11):e79593 PMID: 24244528

Pollard, L. W., Onishi, M., Pringle, J. R., and Lord, M. Fission yeast Cyk3p is a transglutaminase-like protein that participates in cytokinesis and cell morphogenesis. Mol. Biol. Cell, 2012, 23, 2433-2444.

Sammons, M. R., James, M. L., Clayton, J. E., Sladewski, T. E., Sirotkin, V., and Lord, M. A calmodulin-related light chain from fission yeast that functions with myosin-I and PI 4-kinase. J. Cell Sci., 2011, 124, 2466-2477

Kovar, D. R., Sirotkin, V., and Lord, M. ThreeÂ’s company: the fission yeast actin cytoskeleton. Trends Cell Biol., 2011, 21, 177-187.

View all Lord publications here.

* indicates equal contribution

Recent Publications

Clayton JE, Pollard LW, Murray GG, Lord M (2015) Myosin motor isoforms direct specification of actomyosin function by tropomyosins. Cytoskeleton (Hoboken) 72(3): 131-45.

Pollard LW, Lord M (2014) Getting myosin-V on the right track: tropomyosin sorts transport in yeast. Bioarchitecture 4(1): 35-8.

Stark BC, James ML, Pollard LW, Sirotkin V, Lord M (2013) UCS protein Rng3p is essential for myosin-II motor activity during cytokinesis in fission yeast. PLoS One 8(11): e79593.

Clayton JE, Pollard LW, Sckolnick M, Bookwalter CS, Hodges AR, Trybus KM, Lord M (2014) Fission yeast tropomyosin specifies directed transport of myosin-V along actin cables. Mol Biol Cell 25(1): 66-75.

Pollard LW, Onishi M, Pringle JR, Lord M (2012) Fission yeast Cyk3p is a transglutaminase-like protein that participates in cytokinesis and cell morphogenesis. Mol Biol Cell 23(13): 2433-44.

Stark BC, Wen KK, Allingham JS, Rubenstein PA, Lord M (2011) Functional adaptation between yeast actin and its cognate myosin motors. J Biol Chem 286(35): 30384-92.

Sammons MR, James ML, Clayton JE, Sladewski TE, Sirotkin V, Lord M (2011) A calmodulin-related light chain from fission yeast that functions with myosin-I and PI 4-kinase. J Cell Sci 124(Pt 14): 2466-77.

View all Lord publications here.


Matthew Lord, Ph.D.

Matthew
Lord, Ph.D.

Associate Professor
Department of Molecular Physiology & Biophysics

 

802-656-9898
Office: HSRF 108
Lab: HSRF 140

Lab Homepage

Lab Members

   Joe Clayton, CMB Student
   George Murray, Research Technician
   Luther Pollard, CMB Student

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  • 6/16/2015 11:30 AM – 12:30 PM
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    Graham Willsey

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