Role of myosin motors in the cell.
1991-94: BSc Microbiology, University of Sheffield, UK
1994-98: DPhil Biochemistry, University of Oxford, UK
1998-2001: HF Long-term Fellow, Harvard Univ, USA
2001-05: Post-doctoral Fellow, Yale University, USA
2006-present: Assistant Professor, UVM
The long-term goal of our research lies in understanding how the actin-based myosin motors operate in the cell. Our lab focuses on the roles of myosin-I (in endocytosis), myosin-II (in cytokinesis), and myosin-V (in organelle and mRNA transport).
We employ both the fission and budding yeasts as models, taking advantage of the versatile molecular genetics and cell biology approaches on offer in these systems. Current interests lie in regulation of myosins by i) modification of the actin track, and ii) UCS domain proteins.
We utilize a variety of techniques including molecular biology, yeast genetics, and live cell imaging to track actomyosin dynamics (at contractile rings, endocytic patches, and during intracellular transport). We have optimized the purification of myosin-I, -II, and -V from yeast, allowing us to complement our in vivo approaches with biochemical assays to dissect mechanisms of regulation. Knowing how myosin motors are controlled in cells represents an important step in understanding the actomyosin-dependent processes governing cell proliferation and motility in cancer cells.
Contractile ring assembly and constriction visualized by time-lapse fluorescence microscopy of GFP-myosin-II.
Schematic showing the highly conserved collection of proteins involved in actomyosin ring assembly. Myosin-II is highlighted.
Pollard, L. W., Onishi, M., Pringle, J. R., and Lord, M. Fission yeast Cyk3p is a transglutaminase-like protein that participates in cytokinesis and cell morphogenesis. Mol. Biol. Cell, 2012, 23, 2433-2444.
Kovar, D. R., Sirotkin, V., and Lord, M. ThreeÂ’s company: the fission yeast actin cytoskeleton. Trends Cell Biol., 2011, 21, 177-187.
Stark, B. C., Wen, K.-K., Allingham, J. S., Rubenstein, P. A., and Lord, M. Functional adaptation between yeast actin and its cognate myosin motors. J. Biol. Chem., 2011, 286, 30384-30392.
Sammons, M. R., James, M. L., Clayton, J. E., Sladewski, T. E., Sirotkin, V., and Lord, M. A calmodulin-related light chain from fission yeast that functions with myosin-I and PI 4-kinase. J. Cell Sci., 2011, 124, 2466-2477
Clayton, J. E., Sammons, M. R., Stark, B. C., Hodges, A. R., and Lord, M. Differential regulation of unconventional fission yeast myosins via the actin track. Curr. Biol., 2010, 20, 1423-1431.
Stark, B. C., Sladewski, T. E., Pollard, L. W. and Lord, M. Tropomyosin and myosin-II cellular levels promote actomyosin interactions and contractile ring assembly in fission yeast. Mol. Biol. Cell, 2010, 21, 989-1000.
Clayton JE, Sammons MR, Stark BC, Hodges AR, and Lord M. Differential regulation of unconventional fission yeast myosins via the actin track. Curr. Biol. 2010; 20: 1423-1431.
* indicates equal contribution
Pollard LW, Lord M (2014) Getting myosin-V on the right track: tropomyosin sorts transport in yeast. Bioarchitecture 4(1): 35-8.
Stark BC, James ML, Pollard LW, Sirotkin V, Lord M (2013) UCS protein Rng3p is essential for myosin-II motor activity during cytokinesis in fission yeast. PLoS One 8(11): e79593.
Clayton JE, Pollard LW, Sckolnick M, Bookwalter CS, Hodges AR, Trybus KM, Lord M (2014) Fission yeast tropomyosin specifies directed transport of myosin-V along actin cables. Mol Biol Cell 25(1): 66-75.
Pollard LW, Onishi M, Pringle JR, Lord M (2012) Fission yeast Cyk3p is a transglutaminase-like protein that participates in cytokinesis and cell morphogenesis. Mol Biol Cell 23(13): 2433-44.
Stark BC, Wen KK, Allingham JS, Rubenstein PA, Lord M (2011) Functional adaptation between yeast actin and its cognate myosin motors. J Biol Chem 286(35): 30384-92.
Sammons MR, James ML, Clayton JE, Sladewski TE, Sirotkin V, Lord M (2011) A calmodulin-related light chain from fission yeast that functions with myosin-I and PI 4-kinase. J Cell Sci 124(Pt 14): 2466-77.
Lord M (2011) Cytoskeletal regulation: sorting out stress fibers with tropomyosin. Curr Biol 21(7): R255-7.
Department of Molecular Physiology & Biophysics
Office: HSRF 108
Lab: HSRF 140
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