Molecular Mechanisms of Signal Transduction


After receiving a B.S. in Microbiology and a M.S. in Biochemistry I received a Ph.D. in Cell and Developmental Biology from Harvard University in 2001 where I studied core signaling pathways regulating cell survival in the laboratory of John Blenis. I then moved to Seattle where I studied signaling pathways regulating brain development for two years in the laboratory of Jonathan Cooper at the Fred Hutchinson Cancer Research Center. I then moved back to Boston where I completed my postdoctoral training at Harvard Medical School in the laboratory of Steven Gygi where I studied mass spectrometry and proteomics for three years. In 2006 I joined the Department of Biology at the University of Vermont.

Research Description

Research in the Ballif lab is primarily focused on elucidating molecular mechanisms of signal transduction with two major emphases: (A) genetically-defined signaling pathways regulating mammalian brain development and (B) core signaling pathways regulating cell proliferation, growth and survival. In addition to biochemical and cell biological approaches, we employ mass spectrometry-based proteomics as a primary tool to simultaneously monitor hundreds to thousands of proteins, their modifications and their interactions with other proteins following acute signal administration or across developmental stages.

A second focus of our work involves developing and applying proteomic methodology to advance diverse lines of biological inquiry, some of which have had little to no interface with proteomics. This makes these projects both exciting and challenging. These collaborative projects range from the identification of novel human blood group antigens; to the identification of host proteins that interact with arenavirus proteins; to targeted proteomic characterizations of wasps, pitcher plant ecosystems, ants, fruit flies, unicellular ciliates and parasitic protozoa.

Recent Publications

Cheerathodi M, Vincent JJ, Ballif BA (2015) Quantitative comparison of CrkL-SH3 binding proteins from embryonic murine brain and liver: Implications for developmental signaling and the quantification of protein species variants in bottom-up proteomics. J Proteomics 125: 104-11.

Xie Y, Jin Y, Merenick BL, Ding M, Fetalvero KM, Wagner RJ, Mai A, Gleim S, Tucker DF, Birnbaum MJ, Ballif BA, Luciano AK, Sessa WC, Rzucidlo EM, Powell RJ, Hou L, Zhao H, Hwa J, Yu J, Martin KA (2015) Phosphorylation of GATA-6 is required for vascular smooth muscle cell differentiation after mTORC1 inhibition. Sci Signal 8(376): ra44.

Daniels G, Ballif BA, Helias V, Saison C, Grimsley S, Mannessier L, Hustinx H, Lee E, Cartron JP, Peyrard T, Arnaud L (2015) Lack of the nucleoside transporter ENT1 results in the Augustine-null blood type and ectopic mineralization. Blood 125(23): 3651-4.

Perlini LE, Szczurkowska J, Ballif BA, Piccini A, Sacchetti S, Giovedì S, Benfenati F, Cancedda L (2015) Synapsin III acts downstream of semaphorin 3A/CDK5 signaling to regulate radial migration and orientation of pyramidal neurons in vivo. Cell Rep 11(2): 234-48.

Alayev A, Doubleday PF, Berger SM, Ballif BA, Holz MK (2014) Phosphoproteomics reveals resveratrol-dependent inhibition of Akt/mTORC1/S6K1 signaling. J Proteome Res 13(12): 5734-42.

Doubleday PF, Ballif BA (2014) Developmentally-Dynamic Murine Brain Proteomes and Phosphoproteomes Revealed by Quantitative Proteomics. Proteomes 2(2): 197-207.

Galan JA, Geraghty KM, Lavoie G, Kanshin E, Tcherkezian J, Calabrese V, Jeschke GR, Turk BE, Ballif BA, Blenis J, Thibault P, Roux PP (2014) Phosphoproteomic analysis identifies the tumor suppressor PDCD4 as a RSK substrate negatively regulated by 14-3-3. Proc Natl Acad Sci U S A 111(29): E2918-27.

View all Ballif publications here.

Bryan Ballif, Ph.D.

Ballif, Ph.D.

Associate Professor
Department of Biology


Office: 311 Marsh
Lab: 304 Marsh

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Upcoming Events

  • 7/7/2015 11:30 AM – 12:00 PM
    Davis Auditorium
    Laura Solomon
  • 7/7/2015 12:00 PM – 12:30 PM
    Davis Auditorium
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