At the Microscopy and Microanalysis 2013 Annual Meeting, Mr. Pollard gave a talk and won a Student Paper travel award for a paper titled “Tracking the Importance of Tropomyosin in Myosin-II- and Myosin-V-Dependent Processes in Fission Yeast.”
Mr. Pollard also presented a poster at the ASCB 2012 on “Fission yeast tropomyosin promotes directed transport in cells by converting myosin-V into a processive motor,” a paper by Joe Clayton, Luther Pollard, C. Bookwalter, A. Hodges, Dr. Kathy Trybus, and Dr. Matthew Lord. The abstract for this publication is as follows:
“Most class-V myosins studied to date are dimeric motors that walk along actin filaments as single molecules. This processivity relies on a high-duty ratio in which motors spend the majority of their ATPase cycle time in the strong actin-bound ADP state. However, recent studies have shown that a sub-population of myosin-Vs are low duty ratio, nonprocessive motors. Here we employed a low duty ratio fission yeast myosin-V (Myo52p) to investigate the role of tropomyosin on motor function and transport. Decoration of actin filaments with fission yeast tropomyosin (Cdc8p) converts Myo52p into a processive motor. Similarly, Cdc8p-decoration activated the movement of beads coated with small numbers of Myo52p molecules (that mimic in vivo distributions). This regulation was not unique to Cdc8p because budding yeast tropomyosins and two out of three mammalian tropomyosins tested also activated Myo52p processivity. Our findings reveal a novel mechanism of regulation that facilitates sorting of myosin transport to specific actin tracks within the cell.”
Congratulations on your recent endeavors, Luther!
Mr. Pollard is a Cellular, Molecular, and Biomedical Science PhD candidate in Dr. Matthew Lord’s Lab (Molecular Physiology & Biophysics)