Primers used to amplify and sequence Polystichum sequences in the Barrington Lab, University of Vermont

All of the cpDNA sequences listed here routinely yield good data and are variable and useful. We found Leafy to have insufficient variability, and ITS to be too fussy to get sequences from routinely so far. PGI looks very promising at this writing (September 2005).

Sequence	Direction	Location	Sequence (5' to 3')	Designer (request refs)
psbA-trnH(GUG) IGS	Forward	1	gtt atg cat gaa cgt aat gct c	Sang et al.
psbA-trnH(GUG) IGS	Reverse	550	cgc gca tgg tgg att cac aat cc	Tate and Simpson
rbcL	Forward	1	atg tca cca caa aca gaa act aaa gca agt	Zurawski
rbcL	Forward	424	ctg ctt att cta aga ctt tc	Hauk
rbcL	Forward	878	tca tcg tgc aat gca tgc	Hauk
rbcL	Reverse	1379	tca caa gca gca gct agt tca gga ctc	Zurawski
rbcL	Reverse	940	cat gcg taa tgc ttt ggc caa	Wolf
rbcL	Reverse	432	ata agc agg agg gat tcg cag atc	Hasebe
trnL-trnF IGS	Forward	1	ggt tca agt ccc tct atc cc	Taberlet
trnL-trnF IGS	Reverse	380	aat tga act ggt gac acg ag	Taberlet
trnS(GGA)-rps4 IGS + partial gene	Forward	1	tta ccg agg gtt cga atc cct c	Shaw et al.
trnS(GGA)-rps4 IGS + partial gene	Reverse	1000	atg tcs cgt tay cga gga cct	Souza-Chies et al.
ITS			We are using a variety of primers supplied by Kathleen Pryer: contact her.
Leafy intron 2	Forward	1	tga agt gca gca gat gtc aa	Driscoll
Leafy intron 2	Reverse	388	gct agc acc ttt cag ctt gg	Driscoll
PGIc 14F^1	Forward	1	gtg ctt ctg ggt ctt ttg agt g	Ishikawa et al. 2002
PGIc W14F2^2	Forward	1	tgg gtc ttt tga gtg ttt gg	Watano email
PGIc 16R	Reverse	750	gtt gtc cat tag ttc cag gtt ccc c	Ishikawa et al. 2002
PGIc	Forward	~400	1820-18A^3	Ishikawa pers.comm.
PGIc	Reverse	~400	1820-20R	Ishikawa pers.comm

¹14F amplification yielded one band in spring 2005 for across and lonch; amplification was trouble, so set aside.

²7bp downstream of 14F

³We have amplified this short sequence but we are not pursuing it at the moment.